Methanosarcina barkeri (DSM 8687, OCM 89, Wiesmoor)

Name: Methanosarcina barkeri (DSM 8687, OCM 89, Wiesmoor)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7094

Type strain

Strain Designation Wiesmoor

Culture col. no. DSM 8687 OCM 89 Wiesmoor

NCBI tax ID(s) 2208

Special Collections DSMZ

Links

Methanosarcina barkeri Wiesmoor is an anaerobe, mesophilic prokaryote that was isolated from drainage creek, peat bog.

anaerobe mesophilic genome sequence

Name and taxonomic classification

SI-ID 48513

DSM 8687

@ref 20215

Last LPSN update 2025-12-16 09:48:29

Domain Methanobacteriati

Phylum Methanobacteriota

Class Methanosarcinia

Order Methanosarcinales

Family Methanosarcinaceae

Genus Methanosarcina

Species Methanosarcina barkeri

Full scientific name Methanosarcina barkeri Schnellen 1947 (Approved Lists 1980)

BacDive ID	Other strains from **Methanosarcina barkeri** (4)	Type strain
7087	M. barkeri MS, DSM 800, ATCC 43569, JCM 10043, OCM 38, ... (type strain)
7090	M. barkeri DSM 1311, OCM 27
7091	M. barkeri 227, DSM 1538, ATCC 43241, OCM 35
7093	M. barkeri DSM 2948, OCM 86

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	negative	95.8

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
3349	METHANOSARCINA BARKERI MEDIUM (DSMZ Medium 120a)		Medium recipe at MediaDive	Name: METHANOSARCINA BARKERI MEDIUM (DSMZ Medium 120a) Composition: Methanol 15.4688 g/l NaCl 2.19727 g/l Yeast extract 1.95312 g/l Casitone 1.95312 g/l NaHCO3 0.830078 g/l NH4Cl 0.488281 g/l MgSO4 x 7 H2O 0.488281 g/l K2HPO4 0.341797 g/l Na2S x 9 H2O 0.292969 g/l L-Cysteine HCl x H2O 0.292969 g/l CaCl2 x 2 H2O 0.244141 g/l KH2PO4 0.224609 g/l HCl 0.00244141 g/l FeSO4 x 7 H2O 0.00195312 g/l FeCl2 x 4 H2O 0.00146484 g/l Sodium resazurin 0.000488281 g/l CoCl2 x 6 H2O 0.000185547 g/l Pyridoxine hydrochloride 9.76563e-05 g/l MnCl2 x 4 H2O 9.76563e-05 g/l ZnCl2 6.83594e-05 g/l Thiamine HCl 4.88281e-05 g/l Riboflavin 4.88281e-05 g/l Calcium D-(+)-pantothenate 4.88281e-05 g/l Nicotinic acid 4.88281e-05 g/l p-Aminobenzoic acid 4.88281e-05 g/l (DL)-alpha-Lipoic acid 4.88281e-05 g/l Na2MoO4 x 2 H2O 3.51562e-05 g/l NiCl2 x 6 H2O 2.34375e-05 g/l Folic acid 1.95312e-05 g/l Biotin 1.95312e-05 g/l H3BO3 5.85938e-06 g/l CuCl2 x 2 H2O 1.95313e-06 g/l Vitamin B12 9.76563e-07 g/l H2SO4 Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
3349	positive	growth	30	mesophilic

Physiology and metabolism

Oxygen tolerance

3349

Oxygen toleranceanaerobe

Spore formation

@ref	Spore formation	Confidence
125439		97.2

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#River (Creek)
#Environmental	#Terrestrial	#Wetland (Swamp)

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
3349	drainage creek, peat bog	Northern Germany, Wiesmoor	Germany	DEU	Europe

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
3349	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM96998v1 assembly for Methanosarcina barkeri str. Wiesmoor	complete	GCA_000969985	1434109.4	2630968669	1434109	98.8

Genome browser: GCA_000969985

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Methanosarcina barkeri Wiesmoor
IMG: 2630968669

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	97.20	no
125439	motility	BacteriaNetⓘ	no	74.90	no
125439	gram_stain	BacteriaNetⓘ	negative	95.80	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	67.70	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Methanosarcina barkeri str. Wiesmoor Wiesmoor
NCBI: GCA_000969985.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	65.92	no
125438	anaerobic	anaerobicⓘ	yes	82.21	yes
125438	aerobic	aerobicⓘ	no	88.56	yes
125438	spore-forming	spore-formingⓘ	no	85.35	no
125438	thermophilic	thermophileⓘ	no	86.12	yes
125438	flagellated	motile2+ⓘ	no	80.88	no

Literature

Title	Authors	Journal	DOI	Year
Rapid shift in greenhouse forcing of emerging arctic peatlands.	Christensen TR, Scheller J, Scheel M, Rudd DA, Jackowicz-Korczynski M, Mastepanov M, Lopez-Blanco E.	Sci Rep	10.1038/s41598-023-29859-4	2023
Methanogenic Archaea Can Produce Methane in Deliquescence-Driven Mars Analog Environments.	Maus D, Heinz J, Schirmack J, Airo A, Kounaves SP, Wagner D, Schulze-Makuch D.	Sci Rep	10.1038/s41598-019-56267-4	2020

References

#3349	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 8687
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methanosarcina barkeri (DSM 8687, OCM 89, Wiesmoor)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000969985

Genome-based predictions

Predictions

Literature

Literature

References

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