Name: Methanosarcina barkeri MS
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 7087

Type strain:

Species: Methanosarcina barkeri

Strain Designation: MS

Culture col. no.: DSM 800, ATCC 43569, JCM 10043, OCM 38, NBRC 100474, VKM B-1635

Strain history: DSM 800 <-- M. P. Bryant MS.

NCBI tax ID(s): 1298606 (strain), 2208 (species)

SI-ID 354032

DSM 800, JCM 10043, ATCC 43569, VKM B-1635, NBRC 100474, BCRC 16175, CCRC 16175

Other strains from Methanosarcina barkeri

M. barkeri DSM 1311, OCM 27
M. barkeri 227, DSM 1538, ATCC 43241, OCM ...
M. barkeri DSM 2948, OCM 86
M. barkeri Wiesmoor, DSM 8687, OCM 89

Section

Methanosarcina barkeri MS is an anaerobe, mesophilic archaeon that was isolated from anaerobic sewage digester.

anaerobe
mesophilic
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Methanobacteriota*
	Class	"Methanomicrobia"
	Order	*Methanosarcinales*
	Family	*Methanosarcinaceae*
	Genus	*Methanosarcina*
	Species	**Methanosarcina barkeri**
	Full Scientific Name (LPSN)	Methanosarcina barkeri Schnellen 1947 (Approved Lists 1980)

Information on culture and growth conditions Culture and growth conditions

[Ref.: #456]

Culture medium

METHANOSARCINA MEDIUM (DSMZ Medium 120)

[Ref.: #456]

Culture medium growth

[Ref.: #456]

Culture medium link

Medium recipe at MediaDive

[Ref.: #456]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #456]			growth	35 °C
[Ref.: #67770]			growth	37 °C

[Ref.: #456]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #456]

Oxygen tolerance

anaerobe

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #456]	Sample type/isolated from	anaerobic sewage digester
[Ref.: #456]	Geographic location (country and/or sea, region)	Illinois, Urbana
[Ref.: #456]	Country	USA
[Ref.: #456]	Country ISO 3 Code	USA
[Ref.: #456]	Continent	North America

[Ref.: #67770]	Sample type/isolated from	Anaerobic sewage-sludge digester
[Ref.: #67770]	Geographic location (country and/or sea, region)	Urbana, IL
[Ref.: #67770]	Country	USA
[Ref.: #67770]	Country ISO 3 Code	USA
[Ref.: #67770]	Continent	North America
* marker position based on {}

Isolation sources categories

#Engineered	#Biodegradation	#Anaerobic digestor
#Engineered	#Waste	#Wastewater
#Condition	#Anoxic (anaerobic)	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #456]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #456]	Methanosarcina barkeri 16S rRNA gene, strain DSM 800 (T)	AJ012094	1411	ENA	2208 tax ID
[Ref.: #20218]	Enterococcus faecalis clone OCM038 16S ribosomal RNA gene, partial sequence	GU417226	456	ENA	1351 tax ID	*
[Ref.: #20218]	Methanosarcina barkeri strain MS 16S ribosomal RNA gene, partial sequence	JQ346756	1268	ENA	2208 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Methanosarcina barkeri MS	GCA_000970025	complete	GenBank	1434108 tax ID	*
[Ref.: #66792]	Methanosarcina barkeri JCM 10043	GCA_001316305	contig	GenBank	1298606 tax ID	*
[Ref.: #66792]	Methanosarcina barkeri MS	2630968729	complete	JGI IMG/M	1434108 tax ID	*
[Ref.: #66792]	Methanosarcina barkeri JCM 10043	2684622504	draft	JGI IMG/M	1298606 tax ID	*
[Ref.: #66792]	Methanosarcina barkeri MS	1434108.6	plasmid	PATRIC	1434108 tax ID	*
[Ref.: #66792]	Methanosarcina barkeri MS	1434108.4	complete	PATRIC	1434108 tax ID	*

[Ref.: #456]	GC-content	38.8 mol%
[Ref.: #67770]	GC-content	38.8 mol%	Buoyant density centrifugation (BD)
[Ref.: #67770]	GC-content	39 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Methanosarcina barkeri MS MS NCBI: GCA_000970025.1
[Ref.: #69480]	motile	no	91.43	no
[Ref.: #69480]	flagellated	no	96.459	no
[Ref.: #69480]	gram-positive	no	94.54	no
[Ref.: #69480]	anaerobic	yes	97.783	yes
[Ref.: #69480]	aerobic	no	94.068	no
[Ref.: #69480]	halophile	no	87.398	no
[Ref.: #69480]	spore-forming	no	94.499	no
[Ref.: #69480]	thermophile	no	93.661	yes
[Ref.: #69480]	glucose-util	yes	76.54	no
[Ref.: #69480]	glucose-ferment	no	62.806	no

Availability in culture collections External links

[Ref.: #456]

Culture collection no.

DSM 800, ATCC 43569, JCM 10043, OCM 38, NBRC 100474, VKM B-1635

[Ref.: #76543]

SI-ID 354032

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Transcriptome	Different substrate regimes determine transcriptional profiles and gene co-expression in Methanosarcina barkeri (DSM 800).	Lin Q, Fang X, Ho A, Li J, Yan X, Tu B, Li C, Li J, Yao M, Li X	Appl Microbiol Biotechnol	10.1007/s00253-017-8457-4	2017	*
Phylogeny	Methanosarcina spelaei sp. nov., a methanogenic archaeon isolated from a floating biofilm of a subsurface sulphurous lake.	Ganzert L, Schirmack J, Alawi M, Mangelsdorf K, Sand W, Hillebrand-Voiculescu A, Wagner D	Int J Syst Evol Microbiol	10.1099/ijs.0.064956-0	2014	*
Genetics	F0F1-ATPase genes from an archaebacterium, Methanosarcina barkeri.	Sumi M, Yohda M, Koga Y, Yoshida M	Biochem Biophys Res Commun	10.1006/bbrc.1997.7809	1997	*
Metabolism	In vivo 31P- and 13C-NMR studies of ATP synthesis and methane formation by Methanosarcina barkeri.	Santos H, Fareleira P, Toci R, LeGall J, Peck HD Jr, Xavier AV	Eur J Biochem	10.1111/j.1432-1033.1989.tb14663.x	1989	*
Enzymology	Low-spin sulfite reductases: a new homologous group of non-heme iron-siroheme proteins in anaerobic bacteria.	Moura I, Lino AR, Moura JJ, Xavier AV, Fauque G, Peck HD Jr, LeGall J	Biochem Biophys Res Commun	10.1016/s0006-291x(86)80148-6	1986	*
Enzymology	Purification, characterization and redox properties of hydrogenase from Methanosarcina barkeri (DSM 800).	Fauque G, Teixeira M, Moura I, Lespinat PA, Xavier AV, Der Vartanian DV, Peck HD Jr, Le Gall J, Moura JG	Eur J Biochem	10.1111/j.1432-1033.1984.tb08245.x	1984	*
Phylogeny	Amino acid sequence of a 3Fe:3S ferredoxin from the "archaebacterium" Methanosarcina barkeri (DSM 800).	Hausinger RP, Moura I, Moura JJ, Xavier AV, Santos MH, LeGall J, Howard JB	J Biol Chem	S0021-9258(19)45364-7	1982	*

References

#456	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 800

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#76543

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID354032.1 )

* These data were automatically processed and therefore are not curated

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