Methanococcoides alaskense (DSM 17273, NBRC 107632, OCM 775)

Name: Methanococcoides alaskense (DSM 17273, NBRC 107632, OCM 775)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7061

Type strain

Strain Designation AK-5

Culture col. no. DSM 17273 NBRC 107632 OCM 775 AK-5

NCBI tax ID(s) 325778

Special Collections DSMZ

History DSMZ <- M. Kendall, Portland State Univ., USA; AK-5 <- Y. Liu; {1999}

Links

Methanococcoides alaskense AK-5 is an anaerobe archaeon that was isolated from marine sediment.

anaerobe genome sequence 16S sequence Archaea

Name and taxonomic classification

SI-ID 281457

NBRC 107632,DSM 17273

@ref 20215

Last LPSN update 2026-05-29 08:26:33

Domain Archaea

Phylum Methanobacteriota

Class Methanosarcinia

Order Methanosarcinales

Family Methanosarcinaceae

Genus Methanococcoides

Species Methanococcoides alaskense

Full scientific name Methanococcoides alaskense Singh et al. 2005

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	negative	96.898

Colony morphology

6865

Incubation period3-7 days

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
6865	METHANOCOCCOIDES MEDIUM (DSMZ Medium 141c)		Medium recipe at MediaDive	Name: METHANOCOCCOIDES MEDIUM (DSMZ Medium 141c) Composition: NaHCO3 4.89237 g/l Trimethylammonium chloride 4.89237 g/l MgCl2 x 6 H2O 3.91389 g/l Yeast extract 1.95695 g/l Trypticase peptone 1.95695 g/l Na-acetate 0.978474 g/l Na2S x 9 H2O 0.489237 g/l L-Cysteine HCl x H2O 0.489237 g/l KCl 0.332681 g/l NH4Cl 0.244618 g/l K2HPO4 0.136986 g/l MgSO4 x 7 H2O 0.0293542 g/l Nitrilotriacetic acid 0.0146771 g/l NaCl 0.00978474 g/l MnSO4 x H2O 0.00489237 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195695 g/l CoSO4 x 7 H2O 0.00176125 g/l ZnSO4 x 7 H2O 0.00176125 g/l CaCl2 x 2 H2O 0.000978474 g/l FeSO4 x 7 H2O 0.000978474 g/l Sodium resazurin 0.000489237 g/l NiCl2 x 6 H2O 0.000293542 g/l AlK(SO4)2 x 12 H2O 0.000195695 g/l CuSO4 x 5 H2O 9.78474e-05 g/l Pyridoxine hydrochloride 9.78474e-05 g/l H3BO3 9.78474e-05 g/l Na2MoO4 x 2 H2O 9.78474e-05 g/l (DL)-alpha-Lipoic acid 4.89237e-05 g/l Thiamine HCl 4.89237e-05 g/l Riboflavin 4.89237e-05 g/l Nicotinic acid 4.89237e-05 g/l Calcium D-(+)-pantothenate 4.89237e-05 g/l p-Aminobenzoic acid 4.89237e-05 g/l Biotin 1.95695e-05 g/l Folic acid 1.95695e-05 g/l Na2WO4 x 2 H2O 3.91389e-06 g/l Na2SeO3 x 5 H2O 2.93542e-06 g/l Vitamin B12 9.78474e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
6865	positive	growth	20

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
6865	anaerobe
125439	obligate anaerobe	98.981

Spore formation

@ref	Spore formation	Confidence
125439		99.759

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Sediment

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
6865	marine sediment	Alaska, Skan Bay	USA	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence AY941801 (>99% sequence identity) for Methanococcoides from Microbeatlas

Aquatic Samples	2
Soil Samples	0
Animal Samples	0
Plant Samples	0
Total Samples	2

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6865	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	IMG accession	NCBI tax ID	Score
124043	ASM3145418v1 assembly for Methanococcoides alaskense DSM 17273	contig	GCA_031454185	2913378003	325778	74
66792	ASM2759414v1 assembly for Methanococcoides alaskense DSM 17273	contig	GCA_027594145	8036242333	325778	53.86

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
6865	Methanococcoides alaskense strain AK-5 16S ribosomal RNA gene, partial sequence	AY941801	1369		325778

GC content

6865

GC-content (mol%)39.5

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM3145418v1 assembly for Methanococcoides alaskense DSM 17273
NCBI: GCA_031454185

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate anaerobe	98.98	no
125439	gram_stain	BacteriaNetⓘ	negative	96.90	no
125439	motility	BacteriaNetⓘ	no	85.62	no
125439	spore_formation	BacteriaNetⓘ	no	99.76	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Methanococcoides orientis sp. nov., a methylotrophic methanogen isolated from sediment of the East China Sea.	Liang L, Sun Y, Dong Y, Ahmad T, Chen Y, Wang J, Wang F	Int J Syst Evol Microbiol	10.1099/ijsem.0.005384	2022
Phylogeny	Methanococcoides vulcani sp. nov., a marine methylotrophic methanogen that uses betaine, choline and N,N-dimethylethanolamine for methanogenesis, isolated from a mud volcano, and emended description of the genus Methanococcoides.	L'Haridon S, Chalopin M, Colombo D, Toffin L	Int J Syst Evol Microbiol	10.1099/ijs.0.058289-0	2014
Phylogeny	Isolation and characterization of methylotrophic methanogens from anoxic marine sediments in Skan Bay, Alaska: description of Methanococcoides alaskense sp. nov., and emended description of Methanosarcina baltica.	Singh N, Kendall MM, Liu Y, Boone DR	Int J Syst Evol Microbiol	10.1099/ijs.0.63886-0	2005

References

#6865	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17273
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methanococcoides alaskense (DSM 17273, NBRC 107632, OCM 775)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_031454185

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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