Methanimicrococcus blatticola (DSM 13328, ATCC BAA-276, JCM 31471)

Name: Methanimicrococcus blatticola (DSM 13328, ATCC BAA-276, JCM 31471)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7057

Type strain

Strain Designation PA

Culture col. no. DSM 13328 ATCC BAA-276 JCM 31471 PA

NCBI tax ID(s) 91560

Special Collections DSMZ JCM

History <- J. T. Keltjens, University of Nijmegen, Netherlands; PA <- W. W. Sprenger {1999} DSM 13328 <-- J. T. Keltjens; Univ. of Nijmegen, Netherlands; PA.

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Methanimicrococcus blatticola PA is an anaerobe, mesophilic prokaryote that was isolated from hindgut of the American cockroach Periplaneta americana.

anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 49457

JCM 31471,DSM 13328,ATCC BAA-276

@ref 20215

Last LPSN update 2026-02-09 11:19:22

Domain Archaea

Phylum Methanobacteriota

Class Methanosarcinia

Order Methanosarcinales

Family Methanosarcinaceae

Genus Methanimicrococcus

Species Methanimicrococcus blatticola

Full scientific name Methanimicrococcus blatticola corrig. Sprenger et al. 2000

Synonyms (1)

Methanomicrococcus blatticola

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	negative	97

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
4997	METHANOMICROCOCCUS MEDIUM (DSMZ Medium 120b)		Medium recipe at MediaDive	Name: METHANOMICROCOCCUS MEDIUM (DSMZ Medium 120b) Composition: Methanol 15.4688 g/l NaHCO3 2.44141 g/l Na-acetate 2.44141 g/l NaCl 2.19727 g/l Yeast extract 1.95312 g/l Casitone 1.95312 g/l NH4Cl 0.488281 g/l MgSO4 x 7 H2O 0.488281 g/l Na2S x 9 H2O 0.351562 g/l L-Cysteine HCl x H2O 0.351562 g/l K2HPO4 0.341797 g/l CaCl2 x 2 H2O 0.244141 g/l KH2PO4 0.224609 g/l 2-Mercaptoethanesulfonate 0.136719 g/l HCl 0.00244141 g/l FeSO4 x 7 H2O 0.00195312 g/l FeCl2 x 4 H2O 0.00146484 g/l Sodium resazurin 0.000488281 g/l CoCl2 x 6 H2O 0.000185547 g/l Pyridoxine hydrochloride 9.76563e-05 g/l MnCl2 x 4 H2O 9.76563e-05 g/l ZnCl2 6.83594e-05 g/l p-Aminobenzoic acid 4.88281e-05 g/l (DL)-alpha-Lipoic acid 4.88281e-05 g/l Calcium D-(+)-pantothenate 4.88281e-05 g/l Nicotinic acid 4.88281e-05 g/l Riboflavin 4.88281e-05 g/l Thiamine HCl 4.88281e-05 g/l Na2MoO4 x 2 H2O 3.51562e-05 g/l NiCl2 x 6 H2O 2.34375e-05 g/l Biotin 1.95312e-05 g/l Folic acid 1.95312e-05 g/l H3BO3 5.85938e-06 g/l CuCl2 x 2 H2O 1.95313e-06 g/l Vitamin B12 9.76563e-07 g/l H2SO4 Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
4997	positive	growth	35	mesophilic
67770	positive	growth	37	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
4997	anaerobe
125439	aerobe	94.9

Spore formation

@ref	Spore formation	Confidence
125438		91.863
125439		98.9

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Arthropoda	#Insecta
#Host Body-Site	#Gastrointestinal tract	-

Isolation

@ref	Sample type	Host species	Country	Country ISO 3 Code	Continent
4997	hindgut of the American cockroach Periplaneta americana	Periplaneta americana	Netherlands	NLD	Europe
67770	Hindgut of the cockroach Periplaneta americana	Periplaneta americana

Taxonmaps

69479

Global distribution of 16S sequence AJ238002 (>99% sequence identity) for Methanimicrococcus from Microbeatlas

Aquatic Samples	8
Soil Samples	2
Animal Samples	26
Plant Samples	11
Total Samples	47

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
4997	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM2068526v1 assembly for Methanimicrococcus blatticola PA	scaffold	GCA_020685265	91560.14	2698536704	91560	54.99
67770	ASM436321v1 assembly for Methanimicrococcus blatticola DSM 13328	scaffold	GCA_004363215	91560.10	2756170388	91560	54.26
66792	ASM2001155v1 assembly for Methanimicrococcus blatticola DSM 13328	contig	GCA_020011555	91560.13		91560	51.6

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
4997	Methanomicrococcus blatticolus 16S rRNA gene	AJ238002	1453		91560

GC content

4997

GC-content (mol%)55

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Methanimicrococcus blatticola PA
NCBI: GCA_020685265

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	98.90	no
125439	motility	BacteriaNetⓘ	no	74.80	no
125439	gram_stain	BacteriaNetⓘ	negative	97.00	no
125439	oxygen_tolerance	BacteriaNetⓘ	aerobe	94.90	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Methanimicrococcus blatticola PA
NCBI: GCA_020685265.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	62.87	no
125438	anaerobic	anaerobicⓘ	yes	86.01	yes
125438	aerobic	aerobicⓘ	no	90.86	no
125438	spore-forming	spore-formingⓘ	no	91.86	no
125438	thermophilic	thermophileⓘ	no	84.18	yes
125438	flagellated	motile2+ⓘ	no	86.60	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Genome reduction in novel, obligately methyl-reducing Methanosarcinales isolated from arthropod guts (Methanolapillus gen. nov. and Methanimicrococcus).	Protasov E, Reeh H, Liu P, Poehlein A, Platt K, Heimerl T, Herve V, Daniel R, Brune A.	FEMS Microbiol Ecol	10.1093/femsec/fiae111	2024
	Comparative genomic analysis of Methanimicrococcus blatticola provides insights into host adaptation in archaea and the evolution of methanogenesis.	Thomas CM, Taib N, Gribaldo S, Borrel G.	ISME Commun	10.1038/s43705-021-00050-y	2021
Enzymology	Structural Insights into the Methane-Generating Enzyme from a Methoxydotrophic Methanogen Reveal a Restrained Gallery of Post-Translational Modifications.	Kurth JM, Muller MC, Welte CU, Wagner T.	Microorganisms	10.3390/microorganisms9040837	2021
Phylogeny	Methanomicrococcus blatticola gen. nov., sp. nov., a methanol- and methylamine-reducing methanogen from the hindgut of the cockroach Periplaneta americana.	Sprenger WW, van Belzen MC, Rosenberg J, Hackstein JH, Keltjens JT	Int J Syst Evol Microbiol	10.1099/00207713-50-6-1989	2000

References

#4997	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 13328
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methanimicrococcus blatticola (DSM 13328, ATCC BAA-276, JCM 31471)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_020685265

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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