Methanoculleus receptaculi (DSM 18860, CGMCC 1.5087, ZC-2)

Name: Methanoculleus receptaculi (DSM 18860, CGMCC 1.5087, ZC-2)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7031

Type strain

Strain Designation ZC-2

Culture col. no. DSM 18860 CGMCC 1.5087 ZC-2

NCBI tax ID(s) 394967

Special Collections DSMZ

History DSMZ <- T.-L. Qiu and L. Cheng, Biogas Inst. of Ministry of Agriculture, Chengdu, P.R. China; ZC-2 <- L. Cheng; {2005}

Links

Methanoculleus receptaculi ZC-2 is an anaerobe archaeon that was isolated from oil-production water.

anaerobe genome sequence 16S sequence Archaea

Name and taxonomic classification

SI-ID 402078

DSM 18860

@ref 20215

Last LPSN update 2026-05-29 08:26:33

Domain Archaea

Phylum Methanobacteriota

Class "Methanomicrobia"

Order Methanomicrobiales

Family Methanomicrobiaceae

Genus Methanoculleus

Species Methanoculleus receptaculi

Full scientific name Methanoculleus receptaculi Cheng et al. 2008

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
7750	METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141)		Medium recipe at MediaDive	Name: METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141) Composition: NaHCO3 4.89237 g/l MgCl2 x 6 H2O 3.91389 g/l Yeast extract 1.95695 g/l Trypticase peptone 1.95695 g/l Na-acetate 0.978474 g/l Na2S x 9 H2O 0.489237 g/l L-Cysteine HCl x H2O 0.489237 g/l KCl 0.332681 g/l NH4Cl 0.244618 g/l K2HPO4 0.136986 g/l MgSO4 x 7 H2O 0.0293542 g/l Nitrilotriacetic acid 0.0146771 g/l NaCl 0.00978474 g/l MnSO4 x H2O 0.00489237 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195695 g/l CoSO4 x 7 H2O 0.00176125 g/l ZnSO4 x 7 H2O 0.00176125 g/l FeSO4 x 7 H2O 0.000978474 g/l CaCl2 x 2 H2O 0.000978474 g/l Sodium resazurin 0.000489237 g/l NiCl2 x 6 H2O 0.000293542 g/l AlK(SO4)2 x 12 H2O 0.000195695 g/l CuSO4 x 5 H2O 9.78474e-05 g/l Pyridoxine hydrochloride 9.78474e-05 g/l Na2MoO4 x 2 H2O 9.78474e-05 g/l H3BO3 9.78474e-05 g/l Nicotinic acid 4.89237e-05 g/l Riboflavin 4.89237e-05 g/l (DL)-alpha-Lipoic acid 4.89237e-05 g/l Thiamine HCl 4.89237e-05 g/l Calcium D-(+)-pantothenate 4.89237e-05 g/l p-Aminobenzoic acid 4.89237e-05 g/l Folic acid 1.95695e-05 g/l Biotin 1.95695e-05 g/l Na2WO4 x 2 H2O 3.91389e-06 g/l Na2SeO3 x 5 H2O 2.93542e-06 g/l Vitamin B12 9.78474e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
7750	positive	growth	50

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
7750	anaerobe
125439	facultative anaerobe	99.229

Spore formation

@ref	Spore formation	Confidence
125439		99.384

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Industrial	#Oil reservoir
#Environmental	#Aquatic	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
7750	oil-production water	Shengli oil field	China	CHN	Asia

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
7750	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	NCBI tax ID	Score
124043	ASM3347259v1 assembly for Methanoculleus receptaculi DSM 18860	complete	GCA_033472595	394967	90.35

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
7750	Methanoculleus receptaculi strain ZC-2 16S ribosomal RNA gene, partial sequence	DQ787476	1340		394967

GC content

@ref	GC-content (mol%)	Method
7750	55.2	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM3347259v1 assembly for Methanoculleus receptaculi DSM 18860
NCBI: GCA_033472595

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	99.23	no
125439	gram_stain	BacteriaNetⓘ	positive	58.42	no
125439	motility	BacteriaNetⓘ	no	70.51	no
125439	spore_formation	BacteriaNetⓘ	no	99.38	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Isolation and characterization of Methanoculleus receptaculi sp. nov. from Shengli oil field, China.	Cheng L, Qiu TL, Li X, Wang WD, Deng Y, Yin XB, Zhang H	FEMS Microbiol Lett	10.1111/j.1574-6968.2008.01212.x	2008

References

#7750	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18860
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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About us

Methanoculleus receptaculi (DSM 18860, CGMCC 1.5087, ZC-2)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_033472595

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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