Methanofollis liminatans DSM 4140 is an anaerobe, mesophilic prokaryote that was isolated from industrial wastewater treatment plant.
anaerobe mesophilic genome sequence 16S sequence| @ref 20215 |
Last LPSN update
2025-12-16 09:48:28 |
| Domain Methanobacteriati |
| Phylum Methanobacteriota |
| Class "Methanomicrobia" |
| Order Methanomicrobiales |
| Family Methanomicrobiaceae |
| Genus Methanofollis |
| Species Methanofollis liminatans |
| Full scientific name Methanofollis liminatans (Zellner et al. 1990) Zellner et al. 1999 |
| Synonyms (1) |
| BacDive ID | Other strains from Methanofollis liminatans (1) | Type strain |
|---|---|---|
| 7011 | M. liminatans BM1, DSM 10196 |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 1601 | MODIFIED METHANOBACTERIUM MEDIUM (DSMZ Medium 1523) | Medium recipe at MediaDive  | Name: MODIFIED METHANOBACTERIUM MEDIUM (DSMZ Medium 1523; with strain-specific modifications) Composition: Brain heart infusion 5.98802 g/l Proteose peptone 5.98802 g/l Na-acetate 3.99202 g/l NaHCO3 3.99202 g/l Yeast extract 1.99601 g/l Na-formate 1.99601 g/l Na2S x 9 H2O 0.499002 g/l KH2PO4 0.499002 g/l L-Cysteine HCl x H2O 0.499002 g/l MgSO4 x 7 H2O 0.399202 g/l NH4Cl 0.399202 g/l NaCl 0.399202 g/l CaCl2 x 2 H2O 0.0499002 g/l HCl 0.00249501 g/l FeCl2 x 4 H2O 0.00149701 g/l Sodium resazurin 0.000499002 g/l Pyridoxine hydrochloride 0.000299401 g/l Thiamine-HCl x 2 H2O 0.000199601 g/l Nicotinic acid 0.000199601 g/l CoCl2 x 6 H2O 0.000189621 g/l Calcium pantothenate 9.98004e-05 g/l Vitamin B12 9.98004e-05 g/l MnCl2 x 4 H2O 9.98004e-05 g/l p-Aminobenzoic acid 7.98403e-05 g/l ZnCl2 6.98603e-05 g/l Na2MoO4 x 2 H2O 3.59281e-05 g/l NiCl2 x 6 H2O 2.39521e-05 g/l D-(+)-biotin 1.99601e-05 g/l H3BO3 5.98802e-06 g/l CuCl2 x 2 H2O 1.99601e-06 g/l Distilled water |
| @ref | Growth | Type | Temperature (°C) | Range | |
|---|---|---|---|---|---|
| 1601 | positive | growth | 37 | mesophilic |
| Cat1 | Cat2 | Cat3 | |
|---|---|---|---|
| #Engineered | #Waste | #Industrial wastewater | |
| #Engineered | #Waste | #Water treatment plant |
| @ref | Sample type | Geographic location | Country | Country ISO 3 Code | Continent | |
|---|---|---|---|---|---|---|
| 1601 | industrial wastewater treatment plant | Frankfurt/Main | Germany | DEU | Europe |
Global distribution of 16S sequence Y16428 (>99% sequence identity) for Methanofollis from Microbeatlas 
 Aquatic Samples |
71 |
 Soil Samples |
8 |
 Animal Samples |
1 |
 Plant Samples |
9 |
| Total Samples | 89 |
| @ref | Description | Assembly level | INSDC accession | BV-BRC accession | IMG accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|---|---|
| 66792 | ASM27586v1 assembly for Methanofollis liminatans DSM 4140 | chromosome | GCA_000275865   | 28892.12  | 2506783068  | 28892 | 87.53 |  |
| @ref | Description | Accession | Length | Database | NCBI tax ID | |
|---|---|---|---|---|---|---|
| 1601 | Methanofollis liminatans 16S rRNA gene, strain DSM 4140 | Y16428 | 1430 |  | 28892 |
| 1601 | GC-content (mol%)60.0 |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Metabolism | Metabolic versatility of anaerobic sludge towards platform chemical production from waste glycerol. | Magalhaes CP, Alves JI, Duber A, Oleskowicz-Popiel P, Stams AJM, Cavaleiro AJ. | Appl Microbiol Biotechnol | 10.1007/s00253-024-13248-6 | 2024 | |
| The origin of methyl group in methanogen-mediated mercury methylation: From the Wolfe cycle. | Gao J, Yang J, Dong H, Tao S, Shi J, He B, Bian X, Wu JL, Yin Y, Hu L, Jiang G. | Proc Natl Acad Sci U S A | 10.1073/pnas.2416761121 | 2024 | | |
| Genetics | Machine learning classification of archaea and bacteria identifies novel predictive genomic features. | Bobbo T, Biscarini F, Yaddehige SK, Alberghini L, Rigoni D, Bianchi N, Taccioli C. | BMC Genomics | 10.1186/s12864-024-10832-y | 2024 | |
| Microbiological insight into various underground gas storages in Vienna Basin focusing on methanogenic Archaea. | Hanisakova N, Vitezova M, Vitez T, Kushkevych I, Kotrlova E, Novak D, Lochman J, Zavada R. | Front Microbiol | 10.3389/fmicb.2023.1293506 | 2023 | | |
| Metabolism | Development and Validation of Broad-Range Qualitative and Clade-Specific Quantitative Molecular Probes for Assessing Mercury Methylation in the Environment. | Christensen GA, Wymore AM, King AJ, Podar M, Hurt RA, Santillan EU, Soren A, Brandt CC, Brown SD, Palumbo AV, Wall JD, Gilmour CC, Elias DA. | Appl Environ Microbiol | 10.1128/aem.01271-16 | 2016 | |
| Phylogeny | Use of a hierarchical oligonucleotide primer extension approach for multiplexed relative abundance analysis of methanogens in anaerobic digestion systems. | Wu JH, Chuang HP, Hsu MH, Chen WY. | Appl Environ Microbiol | 10.1128/aem.02450-13 | 2013 | |
| Transcriptome | Sequencing intractable DNA to close microbial genomes. | Hurt RA, Brown SD, Podar M, Palumbo AV, Elias DA. | PLoS One | 10.1371/journal.pone.0041295 | 2012 | |
| Phylogeny | Diversity, abundance, and activity of archaeal populations in oil-contaminated groundwater accumulated at the bottom of an underground crude oil storage cavity. | Watanabe K, Kodama Y, Hamamura N, Kaku N. | Appl Environ Microbiol | 10.1128/aem.68.8.3899-3907.2002 | 2002 | |
| Phylogeny | Reclassification of Methanogenium tationis and Methanogenium liminatans as Methanofollis tationis gen. nov., comb. nov. and Methanofollis liminatans comb. nov. and description of a new strain of Methanofollis liminatans. | Zellner G, Boone DR, Keswani J, Whitman WB, Woese CR, Hagelstein A, Tindall BJ, Stackebrandt E | Int J Syst Bacteriol | 10.1099/00207713-49-1-247 | 1999 | |
How to citeIf you want to cite this particular strain cite the following doi:
https://doi.org/10.13145/bacdive7010.20251217.10
When using BacDive for research please cite the following paper
BacDive in 2025: the core database for prokaryotic strain data
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