Methanothermococcus thermolithotrophicus SN-1 is an anaerobe, thermophilic prokaryote that was isolated from geothermally heated sea floor.
anaerobe thermophilic genome sequence 16S sequence| @ref 20215 |
Last LPSN update
2025-12-16 09:48:28 |
| Domain Methanobacteriati |
| Phylum Methanobacteriota |
| Class Methanococci |
| Order Methanococcales |
| Family Methanococcaceae |
| Genus Methanothermococcus |
| Species Methanothermococcus thermolithotrophicus |
| Full scientific name Methanothermococcus thermolithotrophicus (Huber et al. 1984) Whitman 2002 |
| Synonyms (1) |
| BacDive ID | Other strains from Methanothermococcus thermolithotrophicus (1) | Type strain |
|---|---|---|
| 6992 | M. thermolithotrophicus ST22, DSM 8766 |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 719 | METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141) | Medium recipe at MediaDive  | Name: METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141) Composition: NaHCO3 4.89237 g/l MgCl2 x 6 H2O 3.91389 g/l Yeast extract 1.95695 g/l Trypticase peptone 1.95695 g/l Na-acetate 0.978474 g/l Na2S x 9 H2O 0.489237 g/l L-Cysteine HCl x H2O 0.489237 g/l KCl 0.332681 g/l NH4Cl 0.244618 g/l K2HPO4 0.136986 g/l MgSO4 x 7 H2O 0.0293542 g/l Nitrilotriacetic acid 0.0146771 g/l NaCl 0.00978474 g/l MnSO4 x H2O 0.00489237 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195695 g/l CoSO4 x 7 H2O 0.00176125 g/l ZnSO4 x 7 H2O 0.00176125 g/l FeSO4 x 7 H2O 0.000978474 g/l CaCl2 x 2 H2O 0.000978474 g/l Sodium resazurin 0.000489237 g/l NiCl2 x 6 H2O 0.000293542 g/l AlK(SO4)2 x 12 H2O 0.000195695 g/l CuSO4 x 5 H2O 9.78474e-05 g/l Pyridoxine hydrochloride 9.78474e-05 g/l Na2MoO4 x 2 H2O 9.78474e-05 g/l H3BO3 9.78474e-05 g/l Nicotinic acid 4.89237e-05 g/l Riboflavin 4.89237e-05 g/l (DL)-alpha-Lipoic acid 4.89237e-05 g/l Thiamine HCl 4.89237e-05 g/l Calcium D-(+)-pantothenate 4.89237e-05 g/l p-Aminobenzoic acid 4.89237e-05 g/l Folic acid 1.95695e-05 g/l Biotin 1.95695e-05 g/l Na2WO4 x 2 H2O 3.91389e-06 g/l Na2SeO3 x 5 H2O 2.93542e-06 g/l Vitamin B12 9.78474e-07 g/l Distilled water |
| @ref | Description | Assembly level | INSDC accession | BV-BRC accession | IMG accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|---|---|
| 66792 | Mth-DSM-2095-CCS-WG assembly for Methanothermococcus thermolithotrophicus DSM 2095 | complete | GCA_946463545   | 523845.19  | 523845 | 73.75 |  | |
| 67770 | ASM37696v1 assembly for Methanothermococcus thermolithotrophicus DSM 2095 | contig | GCA_000376965 | 523845.5 | 2519103186  | 523845 | 67.68 |
| @ref | Description | Accession | Length | Database | NCBI tax ID | |
|---|---|---|---|---|---|---|
| 719 | Methanothermococcus thermolithotrophicus DSM 2095 16S ribosomal RNA gene, partial sequence | M59128 | 1452 |  | 523845 |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Enzymology | Structural and Spectroscopic Insights into Catalytic Intermediates of a [NiFe]-hydrogenase from Group 3. | Jespersen M, Lorent C, Lemaire ON, Zebger I, Wagner T. | Chembiochem | 10.1002/cbic.202500692 | 2025 | |
| Electron flow in hydrogenotrophic methanogens under nickel limitation. | Nomura S, San Segundo-Acosta P, Protasov E, Kaneko M, Kahnt J, Murphy BJ, Shima S. | Nature | 10.1038/s41586-025-09229-y | 2025 | | |
| Non-thermodynamic factors affect competition between thermophilic chemolithoautotrophs from deep-sea hydrothermal vents. | Kubik BC, Holden JF. | Appl Environ Microbiol | 10.1128/aem.00292-24 | 2024 | | |
| Differences in regulation mechanisms of glutamine synthetases from methanogenic archaea unveiled by structural investigations. | Muller MC, Lemaire ON, Kurth JM, Welte CU, Wagner T. | Commun Biol | 10.1038/s42003-023-05726-w | 2024 | | |
| Assimilatory sulfate reduction in the marine methanogen Methanothermococcus thermolithotrophicus. | Jespersen M, Wagner T. | Nat Microbiol | 10.1038/s41564-023-01398-8 | 2023 | | |
| High-Pressure Microfluidics for Ultra-Fast Microbial Phenotyping. | Cario A, Larzilliere M, Nguyen O, Alain K, Marre S. | Front Microbiol | 10.3389/fmicb.2022.866681 | 2022 | | |
| Biotechnology | Quantitative analysis of amino acid excretion by Methanothermobacter marburgensis under N2-fixing conditions. | Reischl B, Schupp B, Palabikyan H, Steger-Mahnert B, Fink C, Rittmann SKR. | Sci Rep | 10.1038/s41598-025-87686-1 | 2025 | |
| Genetics | Asgard archaeal selenoproteome reveals a roadmap for the archaea-to-eukaryote transition of selenocysteine incorporation machinery. | Huang B, Xiao Y, Zhang Y. | ISME J | 10.1093/ismejo/wrae111 | 2024 | |
| Enzymology | Structural Rearrangements of a Dodecameric Ketol-Acid Reductoisomerase Isolated from a Marine Thermophilic Methanogen. | Lemaire ON, Muller MC, Kahnt J, Wagner T. | Biomolecules | 10.3390/biom11111679 | 2021 | |
| The Molecular Determinants of Thermoadaptation: Methanococcales as a Case Study. | Lecocq M, Groussin M, Gouy M, Brochier-Armanet C. | Mol Biol Evol | 10.1093/molbev/msaa312 | 2021 | | |
| Structural and biochemical elucidation of class I hybrid cluster protein natively extracted from a marine methanogenic archaeon. | Lemaire ON, Belhamri M, Wagner T. | Front Microbiol | 10.3389/fmicb.2023.1179204 | 2023 | | |
| Metabolism | The Oxoglutarate Binding Site and Regulatory Mechanism Are Conserved in Ammonium Transporter Inhibitors GlnKs from Methanococcales. | Muller MC, Wagner T. | Int J Mol Sci | 10.3390/ijms22168631 | 2021 | |
| Enzymology | Structural Insights into the Methane-Generating Enzyme from a Methoxydotrophic Methanogen Reveal a Restrained Gallery of Post-Translational Modifications. | Kurth JM, Muller MC, Welte CU, Wagner T. | Microorganisms | 10.3390/microorganisms9040837 | 2021 | |
| Identification of a novel N-linked glycan on the archaellins and S-layer protein of the thermophilic methanogen, Methanothermococcus thermolithotrophicus. | Kelly JF, Vinogradov E, Stupak J, Robotham AC, Logan SM, Berezuk A, Khursigara CM, Jarrell KF. | J Biol Chem | 10.1074/jbc.ra120.012790 | 2020 | | |
| Genetics | Genomes and secondary metabolomes of Streptomyces spp. isolated from Leontopodium nivale ssp. alpinum. | Malfent F, Zehl M, Kirkegaard RH, Oberhofer M, Zotchev SB. | Front Microbiol | 10.3389/fmicb.2024.1408479 | 2024 | |
| Metabolism | Fluid geochemistry, local hydrology, and metabolic activity define methanogen community size and composition in deep-sea hydrothermal vents. | Stewart LC, Algar CK, Fortunato CS, Larson BI, Vallino JJ, Huber JA, Butterfield DA, Holden JF. | ISME J | 10.1038/s41396-019-0382-3 | 2019 | |
| Genetics | Genomic Variation Influences Methanothermococcus Fitness in Marine Hydrothermal Systems. | Hoffert M, Anderson RE, Reveillaud J, Murphy LG, Stepanauskas R, Huber JA. | Front Microbiol | 10.3389/fmicb.2021.714920 | 2021 | |
| Characterization of TPP-binding proteins in Methanococci archaeal species. | Harris LK. | Bioinformation | 10.6026/97320630012359 | 2016 | | |
| A moderately thermophilic origin of a novel family of marine group II euryarchaeota from deep ocean. | Liu H, Liu WW, Haro-Moreno JM, Xu B, Zheng Y, Liu J, Tian J, Zhang XH, Zhou NY, Qin L, Zhu Y, Rodriguez-Valera F, Zhang C. | iScience | 10.1016/j.isci.2023.107664 | 2023 | | |
| Metabolism | Archaeal acetoacetyl-CoA thiolase/HMG-CoA synthase complex channels the intermediate via a fused CoA-binding site. | Vogeli B, Engilberge S, Girard E, Riobe F, Maury O, Erb TJ, Shima S, Wagner T. | Proc Natl Acad Sci U S A | 10.1073/pnas.1718649115 | 2018 | |
| Phylogenetic and Structural Comparisons of the Three Types of Methyl Coenzyme M Reductase from Methanococcales and Methanobacteriales. | Wagner T, Wegner CE, Kahnt J, Ermler U, Shima S. | J Bacteriol | 10.1128/jb.00197-17 | 2017 | | |
| Structures of the sulfite detoxifying F420-dependent enzyme from Methanococcales. | Jespersen M, Pierik AJ, Wagner T. | Nat Chem Biol | 10.1038/s41589-022-01232-y | 2023 | | |
| In Silico Characterization of Withania coagulans Bioactive Compounds as Potential Inhibitors of Hydroxymethylglutaryl (HMG-CoA) Reductase of Mus musculus. | Azmi MB, Khan F, Asif U, Khurshid B, Wadood A, Qureshi SA, Ahmed SDH, Mudassir HA, Sheikh SI, Feroz N. | ACS Omega | 10.1021/acsomega.2c07893 | 2023 | | |
| Enzymology | Complementation of an aglB Mutant of Methanococcus maripaludis with Heterologous Oligosaccharyltransferases. | Ding Y, Vrionis HA, Schneider J, Berezuk A, Khursigara CM, Jarrell KF. | PLoS One | 10.1371/journal.pone.0167611 | 2016 | |
| Five decades of metalloenzymology. | Hausinger RP. | Enzymes | 10.1016/bs.enz.2023.03.001 | 2023 | | |
| Pathogenicity | Surveillance of Antibiotic Resistance among Hospital- and Community-Acquired Toxigenic Clostridium difficile Isolates over 5-Year Period in Kuwait. | Jamal WY, Rotimi VO. | PLoS One | 10.1371/journal.pone.0161411 | 2016 | |
| Enzymology | Localization of Methyl-Coenzyme M reductase as metabolic marker for diverse methanogenic Archaea. | Wrede C, Walbaum U, Ducki A, Heieren I, Hoppert M. | Archaea | 10.1155/2013/920241 | 2013 | |
| Metabolism | The Prodigal Compound: Return of Ribosyl 1,5-Bisphosphate as an Important Player in Metabolism. | Hove-Jensen B, Brodersen DE, Manav MC. | Microbiol Mol Biol Rev | 10.1128/mmbr.00040-18 | 2019 | |
| Metabolism | SepF is the FtsZ anchor in archaea, with features of an ancestral cell division system. | Pende N, Sogues A, Megrian D, Sartori-Rupp A, England P, Palabikyan H, Rittmann SKR, Grana M, Wehenkel AM, Alzari PM, Gribaldo S. | Nat Commun | 10.1038/s41467-021-23099-8 | 2021 | |
| Metabolism | Role of N,N-Dimethylglycine and Its Catabolism to Sarcosine in Chromohalobacter salexigens DSM 3043. | Yang T, Shao YH, Guo LZ, Meng XL, Yu H, Lu WD. | Appl Environ Microbiol | 10.1128/aem.01186-20 | 2020 | |
| Pathogenicity | Eubacterium lentum ATCC 43055, a new reference strain for quality control of anaerobic susceptibility tests. | Barry AL, Zabransky RJ. | J Clin Microbiol | 10.1128/jcm.28.10.2375-2376.1990 | 1990 | |
| Phylogeny | Estimation of methanogen biomass by quantitation of coenzyme M. | Elias DA, Krumholz LR, Tanner RS, Suflita JM. | Appl Environ Microbiol | 10.1128/aem.65.12.5541-5545.1999 | 1999 | |
| Clinical presentation of leptospirosis: a retrospective study of 34 patients admitted to a single institution in metropolitan France. | Jaureguiberry S, Roussel M, Brinchault-Rabin G, Gacouin A, Le Meur A, Arvieux C, Michelet C, Tattevin P. | Clin Microbiol Infect | 10.1111/j.1469-0691.2005.01148.x | 2005 | | |
| Comparative Transcriptomics Sheds Light on Remodeling of Gene Expression during Diazotrophy in the Thermophilic Methanogen Methanothermococcus thermolithotrophicus. | Maslac N, Sidhu C, Teeling H, Wagner T | mBio | 10.1128/mbio.02443-22 | 2022 | | |
| Phylogeny | Emended description of Actinomyces naeslundii and descriptions of Actinomyces oris sp. nov. and Actinomyces johnsonii sp. nov., previously identified as Actinomyces naeslundii genospecies 1, 2 and WVA 963. | Henssge U, Do T, Radford DR, Gilbert SC, Clark D, Beighton D. | Int J Syst Evol Microbiol | 10.1099/ijs.0.000950-0 | 2009 | |
How to citeIf you want to cite this particular strain cite the following doi:
https://doi.org/10.13145/bacdive7002.20251217.10
When using BacDive for research please cite the following paper
BacDive in 2025: the core database for prokaryotic strain data
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