Methanobrevibacter olleyae (DSM 16632, OCM 841, 1H5-1P)

Name: Methanobrevibacter olleyae (DSM 16632, OCM 841, 1H5-1P)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6977

Type strain

Strain Designation 1H5-1P

Culture col. no. DSM 16632 OCM 841 1H5-1P JCM 39458

NCBI tax ID(s) 294671

Special Collections DSMZ

History <- S. Rea, CSIRO Minerals, Bentley, Australia; 1H5-1P <- K. Munyard

Links

Methanobrevibacter olleyae 1H5-1P is an anaerobe archaeon that was isolated from ovine rumen fluid.

anaerobe genome sequence 16S sequence Archaea

Name and taxonomic classification

SI-ID 402392

JCM 39458,DSM 16632

@ref 20215

Last LPSN update 2026-02-09 11:19:21

Domain Archaea

Phylum Methanobacteriota

Class Methanobacteria

Order Methanobacteriales

Family Methanobacteriaceae

Genus Methanobrevibacter

Species Methanobrevibacter olleyae

Full scientific name Methanobrevibacter olleyae Rea et al. 2007

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	negative	99.9

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
6536	METHANOBACTERIUM MEDIUM (DSMZ Medium 119)		Medium recipe at MediaDive	Name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119; with strain-specific modifications) Composition: NaHCO3 3.98804 g/l Trypticase peptone 1.99402 g/l Na-formate 1.99402 g/l Na-acetate 0.997009 g/l KH2PO4 0.498504 g/l Na2S x 9 H2O 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l NaCl 0.398804 g/l MgSO4 x 7 H2O 0.398804 g/l NH4Cl 0.398804 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Isobutyric acid H2SO4 DL-2-Methylbutyric acid Isovaleric acid Valeric acid Sludge Distilled water
6536	MODIFIED METHANOBACTERIUM MEDIUM (DSMZ Medium 1523)		Medium recipe at MediaDive	Name: MODIFIED METHANOBACTERIUM MEDIUM (DSMZ Medium 1523) Composition: Brain heart infusion 5.98802 g/l Proteose peptone 5.98802 g/l NaHCO3 3.99202 g/l Yeast extract 1.99601 g/l Na-formate 1.99601 g/l Na-acetate 0.998004 g/l Na2S x 9 H2O 0.499002 g/l KH2PO4 0.499002 g/l L-Cysteine HCl x H2O 0.499002 g/l MgSO4 x 7 H2O 0.399202 g/l NH4Cl 0.399202 g/l NaCl 0.399202 g/l CaCl2 x 2 H2O 0.0499002 g/l HCl 0.00249501 g/l FeCl2 x 4 H2O 0.00149701 g/l Sodium resazurin 0.000499002 g/l Pyridoxine hydrochloride 0.000299401 g/l Thiamine-HCl x 2 H2O 0.000199601 g/l Nicotinic acid 0.000199601 g/l CoCl2 x 6 H2O 0.000189621 g/l Calcium pantothenate 9.98004e-05 g/l Vitamin B12 9.98004e-05 g/l MnCl2 x 4 H2O 9.98004e-05 g/l p-Aminobenzoic acid 7.98403e-05 g/l ZnCl2 6.98603e-05 g/l Na2MoO4 x 2 H2O 3.59281e-05 g/l NiCl2 x 6 H2O 2.39521e-05 g/l D-(+)-biotin 1.99601e-05 g/l H3BO3 5.98802e-06 g/l CuCl2 x 2 H2O 1.99601e-06 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
6536	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
6536	anaerobe
125439	facultative anaerobe	96.3

Spore formation

@ref	Spore formation	Confidence
125438		90.92
125439		99.5

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Mammals	#Caprinae (Sheep/Goat)
#Host Body Product	#Fluids	#Rumen fluid

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
6536	ovine rumen fluid	Perth	Australia	AUS	Australia and Oceania

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6536	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	IMG-taxon 2593339150 annotated assembly for Methanobrevibacter olleyae DSM 16632	scaffold	GCA_900114585	294671.4	2593339150	294671	66.42

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
6536	Methanobrevibacter olleyae strain KM1H5-1P 16S ribosomal RNA gene, partial sequence	AY615201	1266		294671

GC content

@ref	GC-content (mol%)	Method
6536	27	sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Methanobrevibacter olleyae DSM 16632
NCBI: GCA_900114585

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	99.50	no
125439	motility	BacteriaNetⓘ	no	54.90	no
125439	gram_stain	BacteriaNetⓘ	negative	99.90	no
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	96.30	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Methanobrevibacter olleyae DSM 16632
NCBI: GCA_900114585.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	58.20	no
125438	anaerobic	anaerobicⓘ	yes	89.85	no
125438	aerobic	aerobicⓘ	no	91.75	no
125438	spore-forming	spore-formingⓘ	no	90.92	no
125438	thermophilic	thermophileⓘ	no	77.85	yes
125438	flagellated	motile2+ⓘ	no	84.71	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Rumen Lachnospiraceae isolate NK3A20 exhibits metabolic flexibility in response to substrate and coculture with a methanogen.	Kaminsky RA, Reid PM, Altermann E, Kenters N, Kelly WJ, Noel SJ, Attwood GT, Janssen PH.	Appl Environ Microbiol	10.1128/aem.00634-23	2023
	Effect of Bile Salt Hydrolase Inhibitors on a Bile Salt Hydrolase from Lactobacillus acidophilus.	Lin J, Negga R, Zeng X, Smith K.	Pathogens	10.3390/pathogens3040947	2014
	Evolving understanding of rumen methanogen ecophysiology.	Khairunisa BH, Heryakusuma C, Ike K, Mukhopadhyay B, Susanti D.	Front Microbiol	10.3389/fmicb.2023.1296008	2023
	Methane Production in Dairy Cows Correlates with Rumen Methanogenic and Bacterial Community Structure.	Danielsson R, Dicksved J, Sun L, Gonda H, Muller B, Schnurer A, Bertilsson J.	Front Microbiol	10.3389/fmicb.2017.00226	2017
Phylogeny	Methanobrevibacter millerae sp. nov. and Methanobrevibacter olleyae sp. nov., methanogens from the ovine and bovine rumen that can utilize formate for growth.	Rea S, Bowman JP, Popovski S, Pimm C, Wright AG	Int J Syst Evol Microbiol	10.1099/ijs.0.63984-0	2007

References

#6536	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16632
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methanobrevibacter olleyae (DSM 16632, OCM 841, 1H5-1P)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_900114585

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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