Methanobrevibacter smithii B181 is an anaerobe archaeon that was isolated from human faeces.
anaerobe genome sequence 16S sequence Archaea| @ref 20215 |
Last LPSN update
2026-02-09 11:19:21 |
| Domain Archaea |
| Phylum Methanobacteriota |
| Class Methanobacteria |
| Order Methanobacteriales |
| Family Methanobacteriaceae |
| Genus Methanobrevibacter |
| Species Methanobrevibacter smithii |
| Full scientific name Methanobrevibacter smithii Balch and Wolfe 1981 |
| Synonyms (1) |
| BacDive ID | Other strains from Methanobrevibacter smithii (3) | Type strain |
|---|---|---|
| 6963 | M. smithii PS, DSM 861, ATCC 35061, JCM 30028, OCM 144 (type strain) | |
| 6964 | M. smithii F1, DSM 2374 | |
| 6965 | M. smithii ALI, DSM 2375 |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 4564 | MODIFIED METHANOBACTERIUM MEDIUM (DSMZ Medium 1523) | Medium recipe at MediaDive  | Name: MODIFIED METHANOBACTERIUM MEDIUM (DSMZ Medium 1523) Composition: Brain heart infusion 5.98802 g/l Proteose peptone 5.98802 g/l NaHCO3 3.99202 g/l Yeast extract 1.99601 g/l Na-formate 1.99601 g/l Na-acetate 0.998004 g/l Na2S x 9 H2O 0.499002 g/l KH2PO4 0.499002 g/l L-Cysteine HCl x H2O 0.499002 g/l MgSO4 x 7 H2O 0.399202 g/l NH4Cl 0.399202 g/l NaCl 0.399202 g/l CaCl2 x 2 H2O 0.0499002 g/l HCl 0.00249501 g/l FeCl2 x 4 H2O 0.00149701 g/l Sodium resazurin 0.000499002 g/l Pyridoxine hydrochloride 0.000299401 g/l Thiamine-HCl x 2 H2O 0.000199601 g/l Nicotinic acid 0.000199601 g/l CoCl2 x 6 H2O 0.000189621 g/l Calcium pantothenate 9.98004e-05 g/l Vitamin B12 9.98004e-05 g/l MnCl2 x 4 H2O 9.98004e-05 g/l p-Aminobenzoic acid 7.98403e-05 g/l ZnCl2 6.98603e-05 g/l Na2MoO4 x 2 H2O 3.59281e-05 g/l NiCl2 x 6 H2O 2.39521e-05 g/l D-(+)-biotin 1.99601e-05 g/l H3BO3 5.98802e-06 g/l CuCl2 x 2 H2O 1.99601e-06 g/l Distilled water | ||
| 4564 | METHANOBACTERIUM MEDIUM (DSMZ Medium 119) | Medium recipe at MediaDive | Name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119) Composition: NaHCO3 3.98804 g/l Na-formate 1.99402 g/l Na-acetate 0.997009 g/l Na2S x 9 H2O 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l KH2PO4 0.498504 g/l NH4Cl 0.398804 g/l NaCl 0.398804 g/l MgSO4 x 7 H2O 0.398804 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Isobutyric acid DL-2-Methylbutyric acid Valeric acid Isovaleric acid H2SO4 Sludge Distilled water |
| @ref | Growth | Type | Temperature (°C) | |
|---|---|---|---|---|
| 4564 | positive | growth | 37 |
| @ref | Description | Assembly level | INSDC accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|
| 124043 | ASM3738951v1 assembly for Methanobrevibacter smithii DSM 11975 B181 | contig | GCA_037389515   | 521001 | 69.5 |  |
| @ref | Description | Accession | Length | Database | NCBI tax ID | |
|---|---|---|---|---|---|---|
| 4564 | Methanobrevibacter smithii B181 16S ribosomal RNA gene, complete sequence | U55235 | 1357 |  | 521001 |
| @ref | GC-content (mol%) | Method | |
|---|---|---|---|
| 4564 | 28.8 | thermal denaturation, midpoint method (Tm) |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Draft genome sequence of Methanobrevibacter smithii strain B181, isolated from a fecal sample. | Plugge CM, Berkhout MD, Galani A, Wang T, Belzer C. | Microbiol Resour Announc | 10.1128/mra.00320-24 | 2024 | | |
| Probiotic Engraftment of Akkermansia muciniphila in an In Vitro Synthetic Microbial Community. | Berkhout MD, de Ram C, Boeren S, Plugge CM, Belzer C. | Microb Ecol | 10.1007/s00248-025-02605-1 | 2025 | | |
| Mucin-driven ecological interactions in an in vitro synthetic community of human gut microbes. | Berkhout MD, Ioannou A, de Ram C, Boeren S, Plugge CM, Belzer C. | Glycobiology | 10.1093/glycob/cwae085 | 2024 | | |
| Methanobrevibacter smithii cell variants in human physiology and pathology: A review. | Malat I, Drancourt M, Grine G. | Heliyon | 10.1016/j.heliyon.2024.e36742 | 2024 | | |
| Lactic Acid Production from Sugarcane Bagasse Hydrolysates by Lactiplantibacillus Strains. | Xavier MCA, Dias GS, Hernalsteens S, Franco TT. | ACS Omega | 10.1021/acsomega.5c08221 | 2025 | | |
| Safety evaluation of the food enzyme triacylglycerol lipase from the genetically modified Komagataella phaffii strain DSM 34125. | EFSA Panel on Food Enzymes (FEZ), Zorn H, Barat Baviera JM, Bolognesi C, Catania F, Gadermaier G, Greiner R, Mayo B, Mortensen A, Roos YH, Solano MLM, Van Loveren H, Vernis L, Aguilera J, Andryszkiewicz M, Cavanna D, Liu Y. | EFSA J | 10.2903/j.efsa.2025.9546 | 2025 | | |
| Phylogeny | Comparative Genomic Analysis of Members of the Genera Methanosphaera and Methanobrevibacter Reveals Distinct Clades with Specific Potential Metabolic Functions. | Poehlein A, Schneider D, Soh M, Daniel R, Seedorf H. | Archaea | 10.1155/2018/7609847 | 2018 | |
| Biological activity of new bioactive steroids deriving from biotransformation of cortisone. | Costa S, Tedeschi P, Ferraro L, Beggiato S, Grandini A, Manfredini S, Buzzi R, Sacchetti G, Valacchi G. | Microb Cell Fact | 10.1186/s12934-022-01967-2 | 2022 | | |
| Dietary selection of metabolically distinct microorganisms drives hydrogen metabolism in ruminants. | Li QS, Wang R, Ma ZY, Zhang XM, Jiao JZ, Zhang ZG, Ungerfeld EM, Yi KL, Zhang BZ, Long L, Long Y, Tao Y, Huang T, Greening C, Tan ZL, Wang M. | ISME J | 10.1038/s41396-022-01294-9 | 2022 | | |
| Enzymology | A novel SfaNI-like restriction-modification system in Caldicellulosiruptor extents the genetic engineering toolbox for this genus. | Swinnen S, Zurek C, Kramer M, Heger RM, Domeyer JE, Ziegler J, Svetlitchnyi VA, Laufer A. | PLoS One | 10.1371/journal.pone.0279562 | 2022 | |
| Genetics | Genomic Characterization of a Mercury Resistant Arthrobacter sp. H-02-3 Reveals the Presence of Heavy Metal and Antibiotic Resistance Determinants | Pathak A, Jaswal R, Chauhan A. | Front Microbiol | 10.3389/fmicb.2019.03039 | 2019 | |
| Genome-resolved metagenomics and metatranscriptomics reveal niche differentiation in functionally redundant microbial communities at deep-sea hydrothermal vents. | Galambos D, Anderson RE, Reveillaud J, Huber JA. | Environ Microbiol | 10.1111/1462-2920.14806 | 2019 | | |
| Enzymology | Anaerobic Biohydrogenation of Isoprene by Acetobacterium wieringae Strain Y. | Jin H, Li X, Wang H, Capiro NL, Li X, Loffler FE, Yan J, Yang Y. | mBio | 10.1128/mbio.02086-22 | 2022 | |
| Pathogenicity | Aberrant intestinal microbiota in individuals with prediabetes. | Allin KH, Tremaroli V, Caesar R, Jensen BAH, Damgaard MTF, Bahl MI, Licht TR, Hansen TH, Nielsen T, Dantoft TM, Linneberg A, Jorgensen T, Vestergaard H, Kristiansen K, Franks PW, IMI-DIRECT consortium, Hansen T, Backhed F, Pedersen O. | Diabetologia | 10.1007/s00125-018-4550-1 | 2018 | |
| Pathogenicity | Colonic mucosal and exfoliome transcriptomic profiling and fecal microbiome response to a flaxseed lignan extract intervention in humans. | Lampe JW, Kim E, Levy L, Davidson LA, Goldsby JS, Miles FL, Navarro SL, Randolph TW, Zhao N, Ivanov I, Kaz AM, Damman C, Hockenbery DM, Hullar MAJ, Chapkin RS. | Am J Clin Nutr | 10.1093/ajcn/nqy325 | 2019 | |
| Pathogenicity | Novel Sulfolobus Fuselloviruses with Extensive Genomic Variations. | Zhang J, Zheng X, Wang H, Jiang H, Dong H, Huang L. | J Virol | 10.1128/jvi.01624-19 | 2020 | |
| Enzymology | Microwave-Assisted Synthesis of Glycoconjugates by Transgalactosylation with Recombinant Thermostable beta-Glycosidase from Pyrococcus. | Henze M, Merker D, Elling L. | Int J Mol Sci | 10.3390/ijms17020210 | 2016 | |
| Dominant Bacterial Phyla from the Human Gut Show Widespread Ability To Transform and Conjugate Bile Acids. | Lucas LN, Barrett K, Kerby RL, Zhang Q, Cattaneo LE, Stevenson D, Rey FE, Amador-Noguez D. | mSystems | 10.1128/msystems.00805-21 | 2021 | | |
| Oligotrophic wetland sediments susceptible to shifts in microbiomes and mercury cycling with dissolved organic matter addition. | Graham EB, Gabor RS, Schooler S, McKnight DM, Nemergut DR, Knelman JE. | PeerJ | 10.7717/peerj.4575 | 2018 | | |
| Metabolism | Purinyl-cobamide is a native prosthetic group of reductive dehalogenases. | Yan J, Bi M, Bourdon AK, Farmer AT, Wang PH, Molenda O, Quaile AT, Jiang N, Yang Y, Yin Y, Simsir B, Campagna SR, Edwards EA, Loffler FE. | Nat Chem Biol | 10.1038/nchembio.2512 | 2018 | |
| Phylogeny | Characterization of nitrifying, denitrifying, and overall bacterial communities in permeable marine sediments of the northeastern Gulf of Mexico. | Mills HJ, Hunter E, Humphrys M, Kerkhof L, McGuinness L, Huettel M, Kostka JE. | Appl Environ Microbiol | 10.1128/aem.02692-07 | 2008 | |
| Microbial community diversity associated with carbon and nitrogen cycling in permeable shelf sediments. | Hunter EM, Mills HJ, Kostka JE. | Appl Environ Microbiol | 10.1128/aem.03007-05 | 2006 | | |
| Phylogeny | Caldalkalibacillus thermarum gen. nov., sp. nov., a novel alkalithermophilic bacterium from a hot spring in China. | Xue Y, Zhang X, Zhou C, Zhao Y, Cowan DA, Heaphy S, Grant WD, Jones BE, Ventosa A, Ma Y. | Int J Syst Evol Microbiol | 10.1099/ijs.0.64105-0 | 2006 | |
| Phylogeny | Bacillus alveayuensis sp. nov., a thermophilic bacterium isolated from deep-sea sediments of the Ayu Trough. | Bae SS, Lee JH, Kim SJ. | Int J Syst Evol Microbiol | 10.1099/ijs.0.63424-0 | 2005 | |
How to citeIf you want to cite this particular strain cite the following doi:
https://doi.org/10.13145/bacdive6966.20251217.10
When using BacDive for research please cite the following paper
BacDive in 2025: the core database for prokaryotic strain data
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