Name: Methanobrevibacter smithii PS
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 6963

Type strain:

Species: Methanobrevibacter smithii

Strain Designation: PS

Culture col. no.: DSM 861, ATCC 35061, JCM 30028, OCM 144

Strain history: DSM 861 <-- M. P. Bryant <-- P. H. Smith strain PS.

NCBI tax ID(s): 420247 (strain), 2173 (species)

SI-ID 40847

ATCC 35061, DSM 861

Special Collections

DSMZ
JCM

Other strains from Methanobrevibacter smithii

Section

Methanobrevibacter smithii PS is an anaerobe, Gram-negative archaeon that was isolated from primary sewage digester.

Gram-negative
anaerobe
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Methanobacteriota*
	Class	*Methanobacteria*
	Order	*Methanobacteriales*
	Family	*Methanobacteriaceae*
	Genus	*Methanobrevibacter*
	Species	**Methanobrevibacter smithii**
	Full Scientific Name (LPSN)	Methanobrevibacter smithii Balch and Wolfe 1981

Information on culture and growth conditions Culture and growth conditions

[Ref.: #464]

Culture medium

MODIFIED METHANOBACTERIUM MEDIUM (DSMZ Medium 1523)

[Ref.: #464]

Culture medium growth

[Ref.: #464]

Culture medium link

Medium recipe at MediaDive

[Ref.: #464]

Culture medium composition

expand / minimize

[Ref.: #464]

Culture medium

METHANOBACTERIUM MEDIUM (DSMZ Medium 119)

[Ref.: #464]

Culture medium growth

[Ref.: #464]

Culture medium link

Medium recipe at MediaDive

[Ref.: #464]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #464]			growth	37 °C
[Ref.: #67770]			growth	37 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #464]

Oxygen tolerance

anaerobe

[Ref.: #69480]	Ability of spore formation	no Confidence in %92.647
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #464]	Sample type/isolated from	primary sewage digester
[Ref.: #464]	Geographic location (country and/or sea, region)	Florida, Gainesville
[Ref.: #464]	Country	USA
[Ref.: #464]	Country ISO 3 Code	USA
[Ref.: #464]	Continent	North America

[Ref.: #67770]	Sample type/isolated from	Primary sewage digester
[Ref.: #67770]	Geographic location (country and/or sea, region)	Gainesville, FL
[Ref.: #67770]	Country	USA
[Ref.: #67770]	Country ISO 3 Code	USA
[Ref.: #67770]	Continent	North America
* marker position based on {}

Isolation sources categories

#Engineered

#Waste

#Wastewater

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #464]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Methanobrevibacter smithii ATCC 35061 culture DSM:861 16S ribosomal RNA gene, partial sequence	JQ346750	1268	GenBank ENA	420247 tax ID	*
[Ref.: #124043]	Methanobrevibacter smithii ATCC 35061 16S ribosomal RNA gene, complete sequence.	U55233	1343	GenBank ENA	420247 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Methanobrevibacter smithii ATCC 35061 ATCC 35061; PS; DSMZ 861	GCA_000016525	complete	GenBank ENA	420247 tax ID	*
[Ref.: #66792]	Methanobrevibacter smithii PS, ATCC 35061	640427121	complete	JGI IMG/M	420247 tax ID	*
[Ref.: #66792]	Methanobrevibacter smithii ATCC 35061	420247.28	complete	PATRIC	420247 tax ID	*
[Ref.: #66792]	Methanobrevibacter smithii ATCC 35061 DSM 861	GCA_028743435	complete	GenBank ENA	420247 tax ID	*

[Ref.: #464]

GC-content

31.0 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Methanobrevibacter smithii ATCC 35061 NCBI: GCA_000016525.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Methanobrevibacter smithii ATCC 35061 NCBI: GCA_000016525.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	56.631	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	yes	89.365	yes
[Ref.: #69480]	aerobic	aerobicⓘ	no	88.442	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	92.647	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	82.254	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	84.208	no

Availability in culture collections External links

[Ref.: #464]

Culture collection no.

DSM 861, ATCC 35061, JCM 30028, OCM 144

[Ref.: #76425]

SI-ID 40847

Literature:

Topic	Title	Authors	Journal	DOI	Year
Cultivation	Mutual Exclusion of Methanobrevibacter Species in the Human Gut Microbiota Facilitates Directed Cultivation of a Candidatus Methanobrevibacter Intestini Representative.	Low A, Lee JKY, Gounot JS, Ravikrishnan A, Ding Y, Saw WY, Tan LWL, Moong DKN, Teo YY, Nagarajan N, Seedorf H	Microbiol Spectr	10.1128/spectrum.00849-22	2022	*
Genetics	Complete Genome Sequence of Methanobrevibacter smithii Strain KB11, Isolated from a Korean Fecal Sample.	Kim BC, Jeong H	Genome Announc	10.1128/genomeA.00038-18	2018	*
Enzymology	Relationship between rumen methanogens and methane production in dairy cows fed diets supplemented with a feed enzyme additive.	Zhou M, Chung YH, Beauchemin KA, Holtshausen L, Oba M, McAllister TA, Guan LL	J Appl Microbiol	10.1111/j.1365-2672.2011.05126.x	2011	*
Metabolism	High prevalence of Methanobrevibacter smithii and Methanosphaera stadtmanae detected in the human gut using an improved DNA detection protocol.	Dridi B, Henry M, El Khechine A, Raoult D, Drancourt M	PLoS One	10.1371/journal.pone.0007063	2009	*
Pathogenicity	Isolation and antimicrobial susceptibility testing of fecal strains of the archaeon Methanobrevibacter smithii.	Dermoumi HL, Ansorg RA	Chemotherapy	10.1159/000063219	2001	*

References

#464	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 861

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#76425

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID40847.1 )

#124043

Dr. Isabel Schober, Dr. Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .

* These data were automatically processed and therefore are not curated

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Methanobrevibacter smithii ATCC 35061

Methanobrevibacter smithii ATCC 35061

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