Name: Methanobrevibacter smithii PS
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6963

Type strain:

Species: Methanobrevibacter smithii

Strain Designation: PS

Culture col. no.: DSM 861, ATCC 35061, JCM 30028, OCM 144

Strain history: DSM 861 <-- M. P. Bryant <-- P. H. Smith strain PS.

NCBI tax ID(s): 420247 (strain), 2173 (species)

SI-ID 40847

ATCC 35061, DSM 861

Other strains from Methanobrevibacter smithii

M. smithii F1, DSM 2374
M. smithii ALI, DSM 2375
M. smithii B181, DSM 11975

Section

Methanobrevibacter smithii PS is an anaerobe, mesophilic, Gram-negative archaeon that was isolated from primary sewage digester.

Gram-negative
anaerobe
mesophilic
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Methanobacteriota*
	Class	*Methanobacteria*
	Order	*Methanobacteriales*
	Family	*Methanobacteriaceae*
	Genus	*Methanobrevibacter*
	Species	**Methanobrevibacter smithii**
	Full Scientific Name (LPSN)	Methanobrevibacter smithii Balch and Wolfe 1981

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.89

Information on culture and growth conditions Culture and growth conditions

[Ref.: #464]

Culture medium

MODIFIED METHANOBACTERIUM MEDIUM (DSMZ Medium 1523)

[Ref.: #464]

Culture medium growth

[Ref.: #464]

Culture medium link

Medium recipe at MediaDive

[Ref.: #464]

Culture medium composition

expand / minimize

[Ref.: #464]

Culture medium

METHANOBACTERIUM MEDIUM (DSMZ Medium 119)

[Ref.: #464]

Culture medium growth

[Ref.: #464]

Culture medium link

Medium recipe at MediaDive

[Ref.: #464]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #464]			growth	37 °C
[Ref.: #67770]			growth	37 °C

[Ref.: #464]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #464]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %99.047

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.891

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #464]	Sample type/isolated from	primary sewage digester
[Ref.: #464]	Geographic location (country and/or sea, region)	Florida, Gainesville
[Ref.: #464]	Country	USA
[Ref.: #464]	Country ISO 3 Code	USA
[Ref.: #464]	Continent	North America

[Ref.: #67770]	Sample type/isolated from	Primary sewage digester
[Ref.: #67770]	Geographic location (country and/or sea, region)	Gainesville, FL
[Ref.: #67770]	Country	USA
[Ref.: #67770]	Country ISO 3 Code	USA
[Ref.: #67770]	Continent	North America
* marker position based on {}

Isolation sources categories

#Engineered

#Waste

#Wastewater

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #464]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Methanobrevibacter smithii ATCC 35061 strain PS 16S ribosomal RNA gene, partial sequence	JQ346750	1268	ENA	420247 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Methanobrevibacter smithii ATCC 35061 ATCC 35061; PS; DSMZ 861	GCA_000016525	complete	GenBank	420247 tax ID	*
[Ref.: #66792]	Methanobrevibacter smithii PS, ATCC 35061	640427121	complete	JGI IMG/M	420247 tax ID	*
[Ref.: #66792]	Methanobrevibacter smithii ATCC 35061	420247.28	complete	PATRIC	420247 tax ID	*
[Ref.: #66792]	Methanobrevibacter smithii ATCC 35061 DSM 861	GCA_028743435	complete	GenBank	420247 tax ID	*

[Ref.: #464]

GC-content

31.0 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Methanobrevibacter smithii ATCC 35061 NCBI: GCA_000016525.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Methanobrevibacter smithii ATCC 35061 NCBI: GCA_000016525.1
[Ref.: #69480]	motile	no	91.61	no
[Ref.: #69480]	flagellated	no	96.13	no
[Ref.: #69480]	gram-positive	no	94.364	no
[Ref.: #69480]	anaerobic	yes	98.975	no
[Ref.: #69480]	halophile	no	76.251	no
[Ref.: #69480]	spore-forming	no	95.973	no
[Ref.: #69480]	thermophile	no	94.216	yes
[Ref.: #69480]	glucose-util	yes	65.152	no
[Ref.: #69480]	aerobic	no	95.902	yes
[Ref.: #69480]	glucose-ferment	no	58.11	no

Availability in culture collections External links

[Ref.: #464]

Culture collection no.

DSM 861, ATCC 35061, JCM 30028, OCM 144

[Ref.: #76425]

SI-ID 40847

Literature:

Topic	Title	Authors	Journal	DOI	Year
Cultivation	Mutual Exclusion of Methanobrevibacter Species in the Human Gut Microbiota Facilitates Directed Cultivation of a Candidatus Methanobrevibacter Intestini Representative.	Low A, Lee JKY, Gounot JS, Ravikrishnan A, Ding Y, Saw WY, Tan LWL, Moong DKN, Teo YY, Nagarajan N, Seedorf H	Microbiol Spectr	10.1128/spectrum.00849-22	2022	*
Genetics	Complete Genome Sequence of Methanobrevibacter smithii Strain KB11, Isolated from a Korean Fecal Sample.	Kim BC, Jeong H	Genome Announc	10.1128/genomeA.00038-18	2018	*
Enzymology	Relationship between rumen methanogens and methane production in dairy cows fed diets supplemented with a feed enzyme additive.	Zhou M, Chung YH, Beauchemin KA, Holtshausen L, Oba M, McAllister TA, Guan LL	J Appl Microbiol	10.1111/j.1365-2672.2011.05126.x	2011	*
Metabolism	High prevalence of Methanobrevibacter smithii and Methanosphaera stadtmanae detected in the human gut using an improved DNA detection protocol.	Dridi B, Henry M, El Khechine A, Raoult D, Drancourt M	PLoS One	10.1371/journal.pone.0007063	2009	*
Pathogenicity	Isolation and antimicrobial susceptibility testing of fecal strains of the archaeon Methanobrevibacter smithii.	Dermoumi HL, Ansorg RA	Chemotherapy	10.1159/000063219	2001	*

References

#464	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 861

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#76425

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID40847.1 )

* These data were automatically processed and therefore are not curated

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Methanobrevibacter smithii ATCC 35061

Methanobrevibacter smithii ATCC 35061

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