Methanobacterium flexile (DSM 25939, AS 1.5092, JCM 15416)

Name: Methanobacterium flexile (DSM 25939, AS 1.5092, JCM 15416)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6954

Type strain

Strain Designation GH, AS.1.5092

Culture col. no. DSM 25939 AS 1.5092 JCM 15416 GH CGMCC 1.5092 1 more

NCBI tax ID(s) 490098

Special Collections DSMZ JCM

History <- T. Itoh, JCM; JCM 15416 <- X. Dong and J. Zhu, Chinese Acad. Sci. (CAS), Inst. Microbiol., Beijing, China; GH X. Dong and J. Zhu strain GH.

Links

Methanobacterium flexile GH is an anaerobe archaeon that was isolated from sediments.

anaerobe 16S sequence Archaea

Name and taxonomic classification

SI-ID 407279

JCM 15416,DSM 25939

@ref 20215

Last LPSN update 2026-02-09 11:19:21

Domain Archaea

Phylum Methanobacteriota

Class Methanobacteria

Order Methanobacteriales

Family Methanobacteriaceae

Genus Methanobacterium

Species Methanobacterium flexile

Full scientific name Methanobacterium flexile Zhu et al. 2011

Morphology

Colony morphology

18131

Incubation period2-3 days

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
18131	METHANOBACTERIUM MEDIUM (DSMZ Medium 119)		Medium recipe at MediaDive	Name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119) Composition: NaHCO3 3.98804 g/l Na-formate 1.99402 g/l Na-acetate 0.997009 g/l Na2S x 9 H2O 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l KH2PO4 0.498504 g/l NH4Cl 0.398804 g/l NaCl 0.398804 g/l MgSO4 x 7 H2O 0.398804 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Isobutyric acid DL-2-Methylbutyric acid Valeric acid Isovaleric acid H2SO4 Sludge Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
18131	positive	growth	37
67770	positive	growth	37

Physiology and metabolism

Oxygen tolerance

18131

Oxygen toleranceanaerobe

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Sediment

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
18131	sediments	Qinghai, Gahai lake in the Qaidam basin	China	CHN	Asia
67770	Sediments of Gahai lake in the Qaidam basin	Qinghai	China	CHN	Asia

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
18131	1	Risk group (German classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
18131	Methanobacterium flexile strain GH 16S ribosomal RNA gene, partial sequence	EU333914	1444		490098

GC content

@ref	GC-content (mol%)	Method
18131	36.4	thermal denaturation, midpoint method (Tm)
67770	37.3	high performance liquid chromatography (HPLC)

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Methanobacterium movens sp. nov. and Methanobacterium flexile sp. nov., isolated from lake sediment.	Zhu J, Liu X, Dong X	Int J Syst Evol Microbiol	10.1099/ijs.0.027540-0	2011

References

#18131	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 25939
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methanobacterium flexile (DSM 25939, AS 1.5092, JCM 15416)

Name and taxonomic classification

Morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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