Name: Methanobacterium petrolearium Mic5c12
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 6952

Type strain:

Species: Methanobacterium petrolearium

Strain Designation: Mic5c12

Culture col. no.: DSM 22353, NBRC 105198

Strain history: <- K. Mori, NITE-NBRC; NBRC 105198 <- K. Mori; Mic5c12

NCBI tax ID(s): 710190 (species)

SI-ID 402151

DSM 22353

Special Collections

Section

Methanobacterium petrolearium Mic5c12 is an anaerobe, mesophilic, Gram-variable archaeon that was isolated from oil sludge.

Gram-variable
rod-shaped
anaerobe
mesophilic
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Archaea*
	Phylum	*Methanobacteriota*
	Class	*Methanobacteria*
	Order	*Methanobacteriales*
	Family	*Methanobacteriaceae*
	Genus	*Methanobacterium*
	Species	**Methanobacterium petrolearium**
	Full Scientific Name (LPSN)	Methanobacterium petrolearium Mori and Harayama 2011

Information on morphological and physiological properties Morphology

[Ref.: #29854]	Gram stain	variable

[Ref.: #29854]	Cell length	3.55 µm

[Ref.: #29854]	Cell width	0.3 µm

[Ref.: #29854]	Cell shape	rod-shaped

[Ref.: #29854]	Motility	no

[Ref.: #16309]

Incubation period

3-7 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16309]

Culture medium

METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141)

[Ref.: #16309]

Culture medium growth

[Ref.: #16309]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16309]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #16309]	growth	35 °C
[Ref.: #29854]	growth	20-40 °C
[Ref.: #29854]	optimum	35 °C

	pH	Kind of pH		pH
[Ref.: #29854]			optimum	6.5
[Ref.: #29854]			growth	5.5-9.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #16309]	Oxygen tolerance	anaerobe
[Ref.: #29854]	Oxygen tolerance	anaerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #29854]		NaCl		growth	0-6.96	%
[Ref.: #29854]		NaCl		optimum	1.972	%

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16309]	Sample type/isolated from	oil sludge
[Ref.: #16309]	Geographic location (country and/or sea, region)	Kagoshima
[Ref.: #16309]	Country	Japan
[Ref.: #16309]	Country ISO 3 Code	JPN
[Ref.: #16309]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Industrial	#Oil reservoir
#Environmental	#Terrestrial	#Mud (Sludge)

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16309]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16309]	Methanobacterium petrolearium gene for 16S ribosomal RNA, partial sequence	AB542742	1392	GenBank ENA	710190 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Methanobacterium petrolearium DSM 22353	GCA_017873625	contig	GenBank ENA	710190 tax ID	*
[Ref.: #66792]	Methanobacterium petrolearium DSM 22353	2913397459	draft	JGI IMG/M	710190 tax ID	*

[Ref.: #16309]	GC-content	38.3 mol%	high performance liquid chromatography (HPLC)
[Ref.: #29854]	GC-content	38.3 mol%

Availability in culture collections External links

[Ref.: #16309]

Culture collection no.

DSM 22353, NBRC 105198

[Ref.: #76414]

SI-ID 402151

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Methanobacterium petrolearium sp. nov. and Methanobacterium ferruginis sp. nov., mesophilic methanogens isolated from salty environments.	Mori K, Harayama S	Int J Syst Evol Microbiol	10.1099/ijs.0.022723-0	2010	*

References

#16309

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22353

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#29854

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26228 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#76414

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID402151.1 )

#26228

IJSEM 138 2011 ( DOI 10.1099/ijs.0.022723-0 , PubMed 20173004 )

* These data were automatically processed and therefore are not curated

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