Name: Methanobacterium subterraneum A8p
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6943

Type strain:

Species: Methanobacterium subterraneum

Strain Designation: A8p

Culture col. no.: DSM 11074, ATCC 700657, NBRC 105231

Strain history: <- S. Kotelnikova, Russian Academy of Sciences, Institute of Biochemistry and Physiology of Microorganisms, Moscow, Russia; A8p <- S. Kotelnikova {1995}

NCBI tax ID(s): 59277 (species)

SI-ID 46247

ATCC 700657, DSM 11074

Other strains from Methanobacterium subterraneum

M. subterraneum C2BIS, DSM 11075

Section

Methanobacterium subterraneum A8p is an anaerobe, mesophilic, Gram-negative archaeon that was isolated from groundwater of rock tunnel at 70 m depth..

Gram-negative
anaerobe
mesophilic
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Methanobacteriota*
	Class	*Methanobacteria*
	Order	*Methanobacteriales*
	Family	*Methanobacteriaceae*
	Genus	*Methanobacterium*
	Species	**Methanobacterium subterraneum**
	Full Scientific Name (LPSN)	Methanobacterium subterraneum Kotelnikova et al. 1998

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.914

Information on culture and growth conditions Culture and growth conditions

[Ref.: #4189]

Culture medium

METHANOBACTERIUM SUBTERRANEUM MEDIUM (DSMZ Medium 814)

[Ref.: #4189]

Culture medium growth

[Ref.: #4189]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4189]

Culture medium composition

expand / minimize

[Ref.: #4189]

Culture medium

METHANOBACTERIUM MEDIUM (DSMZ Medium 119)

[Ref.: #4189]

Culture medium growth

[Ref.: #4189]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4189]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #4189]			growth	37 °C

[Ref.: #4189]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #4189]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %99.312

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.246

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #4189]	Sample type/isolated from	groundwater of rock tunnel at 70 m depth.
[Ref.: #4189]	Geographic location (country and/or sea, region)	Äspö Hard Rock Tunnel Laboratory
[Ref.: #4189]	Country	Sweden
[Ref.: #4189]	Country ISO 3 Code	SWE
[Ref.: #4189]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Groundwater

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence X99044 (>99% sequence identity) for Methanobacterium subterraneum subclade from Microbeatlas

Aquatic Samples	222
Soil Samples	32
Animal Samples	13
Plant Samples	20
Total Samples	287

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #4189]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #4189]	Methanobacterium subterraneum 16S rRNA gene, strain A8p	X99044	1436	ENA	59277 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Methanobacterium subterraneum A8p	GCA_002813695	complete	GenBank	59277 tax ID	*
[Ref.: #66792]	Methanobacterium subterraneum A8p	2775506920	complete	JGI IMG/M	59277 tax ID	*
[Ref.: #66792]	Methanobacterium subterraneum strain A8p	59277.3	complete	PATRIC	59277 tax ID	*

[Ref.: #4189]

GC-content

54.5 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Methanobacterium subterraneum A8p NCBI: GCA_002813695.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Methanobacterium subterraneum A8p NCBI: GCA_002813695.1
[Ref.: #69480]	flagellated	no	96.647	no
[Ref.: #69480]	gram-positive	no	96.84	no
[Ref.: #69480]	anaerobic	yes	98.27	yes
[Ref.: #69480]	halophile	no	78.45	no
[Ref.: #69480]	spore-forming	no	94.638	no
[Ref.: #69480]	thermophile	no	93.886	yes
[Ref.: #69480]	glucose-util	yes	70.229	no
[Ref.: #69480]	aerobic	no	95.122	yes
[Ref.: #69480]	motile	no	88.806	no
[Ref.: #69480]	glucose-ferment	no	67.794	no

Availability in culture collections External links

[Ref.: #4189]

Culture collection no.

DSM 11074, ATCC 700657, NBRC 105231

[Ref.: #76405]

SI-ID 46247

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Methanobacterium kanagiense sp. nov., a hydrogenotrophic methanogen, isolated from rice-field soil.	Kitamura K, Fujita T, Akada S, Tonouchi A	Int J Syst Evol Microbiol	10.1099/ijs.0.026013-0	2010	*
Phylogeny	Methanobacterium subterraneum sp. nov., a new alkaliphilic, eurythermic and halotolerant methanogen isolated from deep granitic groundwater.	Kotelnikova S, Macario AJ, Pedersen K	Int J Syst Bacteriol	10.1099/00207713-48-2-357	1998	*

References

#4189

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 11074

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#76405

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID46247.1 )

* These data were automatically processed and therefore are not curated

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