Name: Methanothermobacter crinale Tm2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6899

Type strain:

Species: Methanothermobacter crinale

Strain Designation: Tm2

Culture col. no.: DSM 24598, ACCC 00699, JCM 17393

Strain history: Q. Li Tm2.

NCBI tax ID(s): 1045764 (species)

SI-ID 405658

DSM 24598

Section

Methanothermobacter crinale Tm2 is an anaerobe, thermophilic archaeon that was isolated from crude oil contaminated soil.

anaerobe
thermophilic
16S sequence
Archaea

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Methanobacteriota*
	Class	*Methanobacteria*
	Order	*Methanobacteriales*
	Family	*Methanobacteriaceae*
	Genus	*Methanothermobacter*
	Species	**Methanothermobacter crinale**
	Full Scientific Name (LPSN)	Methanothermobacter crinale Cheng et al. 2012

Information on morphological and physiological properties Morphology

[Ref.: #17704]

Incubation period

3-7 days

Multimedia content

[Ref.: #17704]

Caption:

Medium 119, 65°C

[Ref.: #17704]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #17704]

Culture medium

METHANOBACTERIUM MEDIUM (DSMZ Medium 119)

[Ref.: #17704]

Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #17704]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #17704]			growth	65 °C
[Ref.: #67770]			growth	65 °C

[Ref.: #17704]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #17704]

Oxygen tolerance

anaerobe

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #17704]	Sample type/isolated from	crude oil contaminated soil
[Ref.: #17704]	Geographic location (country and/or sea, region)	Shengli oil fields
[Ref.: #17704]	Country	China
[Ref.: #17704]	Country ISO 3 Code	CHN
[Ref.: #17704]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Oil sand in the Shengli oil field
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Contamination	#Oil (Fuel)
#Engineered	#Industrial	#Oil reservoir
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence HQ283273 (>99% sequence identity) for Methanothermobacter crinale subclade from Microbeatlas

Aquatic Samples	112
Soil Samples	18
Animal Samples	2
Plant Samples	1
Total Samples	133

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #17704]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #17704]	Methanothermobacter crinale strain Tm2 16S ribosomal RNA gene, partial sequence	HQ283273	1451	ENA	1045764 tax ID

[Ref.: #17704]	GC-content	41.1 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67770]	GC-content	40.1 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67770]	GC-content	41.1 mol%	high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #17704]

Culture collection no.

DSM 24598, ACCC 00699, JCM 17393

[Ref.: #76363]

SI-ID 405658

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Isolation and characterization of Methanothermobacter crinale sp. nov., a novel hydrogenotrophic methanogen from the Shengli oil field.	Cheng L, Dai L, Li X, Zhang H, Lu Y	Appl Environ Microbiol	10.1128/AEM.00210-11	2011	*

References

#17704

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 24598

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#76363

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID405658.1 )

* These data were automatically processed and therefore are not curated

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