Methanothermobacter crinale (DSM 24598, ACCC 00699, JCM 17393)

Colony morphology

Multimedia

• @ref:	17704
  multimedia content:	DSM_24598.jpg
  multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_24598.jpg
  caption:	Medium 119, 65°C
  intellectual property rights:	© Leibniz-Institut DSMZ
  manual_annotation:	1

Culture medium

@ref	Name	Growth	Medium link	Composition
17704	METHANOBACTERIUM MEDIUM (DSMZ Medium 119)		Medium recipe at MediaDive	Name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119) Composition: NaHCO3 3.98804 g/l Na-formate 1.99402 g/l Na-acetate 0.997009 g/l Na2S x 9 H2O 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l KH2PO4 0.498504 g/l NH4Cl 0.398804 g/l NaCl 0.398804 g/l MgSO4 x 7 H2O 0.398804 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Isobutyric acid DL-2-Methylbutyric acid Valeric acid Isovaleric acid H2SO4 Sludge Distilled water

Culture temp

Oxygen tolerance

Isolation source categories

Isolation

Taxonmaps

69479

Global distribution of 16S sequence HQ283273 (>99% sequence identity) for Methanothermobacter crinale subclade from Microbeatlas

Aquatic Samples	112
Soil Samples	18
Animal Samples	2
Plant Samples	1
Total Samples	133

Risk assessment

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
17704	Methanothermobacter crinale strain Tm2 16S ribosomal RNA gene, partial sequence	HQ283273	1451		1045764

GC content

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Isolation and characterization of Methanothermobacter crinale sp. nov., a novel hydrogenotrophic methanogen from the Shengli oil field.	Cheng L, Dai L, Li X, Zhang H, Lu Y	Appl Environ Microbiol	10.1128/AEM.00210-11	2011