Methanothermobacter marburgensis (DSM 2133, NBRC 100331, OCM 82)

Name: Methanothermobacter marburgensis (DSM 2133, NBRC 100331, OCM 82)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6898

Type strain

Strain Designation Marburg

Culture col. no. DSM 2133 NBRC 100331 OCM 82 Marburg JCM 14651

NCBI tax ID(s) 145263

Special Collections DSMZ JCM

History <- R. K. Thauer, Univ. Marburg; Marburg <- G. Fuchs NBRC 100331 <-- DSM 2133 <-- R. K. Thauer strain Marburg.

Links

Methanothermobacter marburgensis Marburg is an anaerobe archaeon that was isolated from anaerobic sewage digester.

anaerobe genome sequence 16S sequence Archaea

Name and taxonomic classification

SI-ID 47548

DSM 2133,NBRC 100331,ATCC BAA-927,JCM 14651

@ref 20215

Last LPSN update 2026-02-09 11:19:20

Domain Archaea

Phylum Methanobacteriota

Class Methanobacteria

Order Methanobacteriales

Family Methanobacteriaceae

Genus Methanothermobacter

Species Methanothermobacter marburgensis

Full scientific name Methanothermobacter marburgensis Wasserfallen et al. 2000

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
972	METHANOBACTERIUM MEDIUM (DSMZ Medium 119)		Medium recipe at MediaDive	Name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119) Composition: NaHCO3 3.98804 g/l Na-formate 1.99402 g/l Na-acetate 0.997009 g/l Na2S x 9 H2O 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l KH2PO4 0.498504 g/l NH4Cl 0.398804 g/l NaCl 0.398804 g/l MgSO4 x 7 H2O 0.398804 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Isobutyric acid DL-2-Methylbutyric acid Valeric acid Isovaleric acid H2SO4 Sludge Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
972	positive	growth	65
67770	positive	growth	65

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
972	anaerobe
125439	anaerobe	99.9

Spore formation

@ref	Spore formation	Confidence
125438		91.271

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Biodegradation	#Anaerobic digestor
#Engineered	#Waste	#Wastewater
#Condition	#Anoxic (anaerobic)	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
972	anaerobic sewage digester	Marburg	Germany	DEU	Europe
67770	Anaerobic sewage digester	Marburg	Germany	DEU	Europe

Taxonmaps

69479

Global distribution of 16S sequence X15364 (>99% sequence identity) for Methanothermobacter marburgensis from Microbeatlas

Aquatic Samples	185
Soil Samples	16
Animal Samples	22
Plant Samples	34
Total Samples	257

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
972	1	Risk group (German classification) According to TRBA 466

Phages

@ref	Name	Strain number
124042	psi M2 (Psimunavirus psiM2)	DSM 2133

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM14529v1 assembly for Methanothermobacter marburgensis str. Marburg	complete	GCA_000145295	79929.8	648028041	79929	98.27
66792	ASM2755506v1 assembly for Methanothermobacter marburgensis Marburg	complete	GCA_027555065		8066314433	145263	97.69

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Methanobacterium thermoautotrophicum str. Marburg 16S small subunit ribosomal RNA gene, partial sequence	AF095262	1432		79929
972	Methanothermobacter marburgensis rRNA operon, type strain Marburg	X15364	8709		145263

GC content

972

GC-content (mol%)47.6

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Methanothermobacter marburgensis str. Marburg
NCBI: GCA_000145295

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	72.60	no
125439	motility	BacteriaNetⓘ	no	62.50	no
125439	gram_stain	BacteriaNetⓘ	variable	71.00	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	99.90	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Methanothermobacter marburgensis str. Marburg Marburg
NCBI: GCA_000145295.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	51.79	no
125438	anaerobic	anaerobicⓘ	yes	89.15	yes
125438	aerobic	aerobicⓘ	no	89.16	no
125438	spore-forming	spore-formingⓘ	no	91.27	no
125438	thermophilic	thermophileⓘ	yes	62.12	yes
125438	flagellated	motile2+ⓘ	no	89.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Cultivation	A high-pressure bioreactor system for the cultivation of Methanothermobacter marburgensis on advanced growth media for sustainable energy applications.	Orthofer M, Hofmann W, Rittmann SKR, Paulik C.	Sci Rep	10.1038/s41598-025-19544-z	2025
Biotechnology	Markerless mutagenesis enables isoleucine biosynthesis solely from threonine in Methanothermobacter marburgensis.	Klein M, Hilts AS, Fennessy RT, Trattnig N, Stehrer-Polasek T, Rittmann SK-MR, Fink C.	Microbiol Spectr	10.1128/spectrum.03068-24	2025
Biotechnology	Quantitative analysis of amino acid excretion by Methanothermobacter marburgensis under N₂-fixing conditions.	Reischl B, Schupp B, Palabikyan H, Steger-Mahnert B, Fink C, Rittmann SKR.	Sci Rep	10.1038/s41598-025-87686-1	2025
	Effects of CO₂ and H₂ limitations on Methanococcus maripaludis.	Xue J, Deutzmann JS, Matis N, Kracke F, Spormann A, Gu W.	Microbiol Spectr	10.1128/spectrum.00359-25	2025
	Comparison of Various Reducing Agents for Methane Production by Methanothermobacter marburgensis.	Mock MP, Ochi R, Bieringer M, Bieringer T, Brotsack R, Leyer S.	Microorganisms	10.3390/microorganisms11102533	2023
	Electron flow in hydrogenotrophic methanogens under nickel limitation.	Nomura S, San Segundo-Acosta P, Protasov E, Kaneko M, Kahnt J, Murphy BJ, Shima S.	Nature	10.1038/s41586-025-09229-y	2025
Biotechnology	Method for automated high performance closed batch cultivation of gas-utilizing methanogens.	Hofmann W, Orthofer M, Salas Wallach N, Ruddyard A, Ungerank M, Paulik C, Rittmann SKR.	AMB Express	10.1186/s13568-025-01872-y	2025
Biotechnology	Lipidomics and Comparative Metabolite Excretion Analysis of Methanogenic Archaea Reveal Organism-Specific Adaptations to Varying Temperatures and Substrate Concentrations.	Taubner RS, Baumann LMF, Steiner M, Pfeifer K, Reischl B, Korynt K, Bauersachs T, Mahnert B, Clifford EL, Peckmann J, Schuster B, Birgel D, Rittmann SKR.	mSystems	10.1128/msystems.01159-22	2023
	Development of a simultaneous bioreactor system for characterization of gas production kinetics of methanogenic archaea at high pressure.	Pappenreiter PA, Zwirtmayr S, Mauerhofer LM, Rittmann SKR, Paulik C.	Eng Life Sci	10.1002/elsc.201900035	2019
Genetics	Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism.	Liesegang H, Kaster AK, Wiezer A, Goenrich M, Wollherr A, Seedorf H, Gottschalk G, Thauer RK.	J Bacteriol	10.1128/jb.00844-10	2010
	An integrated systems biology approach reveals differences in formate metabolism in the genus Methanothermobacter.	Casini I, McCubbin T, Esquivel-Elizondo S, Luque GG, Evseeva D, Fink C, Beblawy S, Youngblut ND, Aristilde L, Huson DH, Drager A, Ley RE, Marcellin E, Angenent LT, Molitor B.	iScience	10.1016/j.isci.2023.108016	2023
Enzymology	Localization of Methyl-Coenzyme M reductase as metabolic marker for diverse methanogenic Archaea.	Wrede C, Walbaum U, Ducki A, Heieren I, Hoppert M.	Archaea	10.1155/2013/920241	2013
	Proteomic Analysis of the Hydrogen and Carbon Monoxide Metabolism of Methanothermobacter marburgensis.	Diender M, Pereira R, Wessels HJ, Stams AJ, Sousa DZ.	Front Microbiol	10.3389/fmicb.2016.01049	2016
	Method for Indirect Quantification of CH4 Production via H2O Production Using Hydrogenotrophic Methanogens.	Taubner RS, Rittmann SK.	Front Microbiol	10.3389/fmicb.2016.00532	2016
	Physiology and methane productivity of Methanobacterium thermaggregans.	Mauerhofer LM, Reischl B, Schmider T, Schupp B, Nagy K, Pappenreiter P, Zwirtmayr S, Schuster B, Bernacchi S, Seifert AH, Paulik C, Rittmann SKR.	Appl Microbiol Biotechnol	10.1007/s00253-018-9183-2	2018
Enzymology	De novo modeling of the F(420)-reducing [NiFe]-hydrogenase from a methanogenic archaeon by cryo-electron microscopy.	Mills DJ, Vitt S, Strauss M, Shima S, Vonck J.	Elife	10.7554/elife.00218	2013
	Identification of a protein responsible for the synthesis of archaeal membrane-spanning GDGT lipids.	Zeng Z, Chen H, Yang H, Chen Y, Yang W, Feng X, Pei H, Welander PV.	Nat Commun	10.1038/s41467-022-29264-x	2022
	Membrane Lipid Composition and Amino Acid Excretion Patterns of Methanothermococcus okinawensis Grown in the Presence of Inhibitors Detected in the Enceladian Plume.	Taubner RS, Baumann LMF, Bauersachs T, Clifford EL, Mahnert B, Reischl B, Seifert R, Peckmann J, Rittmann SKR, Birgel D.	Life (Basel)	10.3390/life9040085	2019
Metabolism	Coupling of ferredoxin and heterodisulfide reduction via electron bifurcation in hydrogenotrophic methanogenic archaea.	Kaster AK, Moll J, Parey K, Thauer RK.	Proc Natl Acad Sci U S A	10.1073/pnas.1016761108	2011
Phylogeny	Vertebrate host phylogeny influences gut archaeal diversity.	Youngblut ND, Reischer GH, Dauser S, Maisch S, Walzer C, Stalder G, Farnleitner AH, Ley RE.	Nat Microbiol	10.1038/s41564-021-00980-2	2021
	Biological methane production under putative Enceladus-like conditions.	Taubner RS, Pappenreiter P, Zwicker J, Smrzka D, Pruckner C, Kolar P, Bernacchi S, Seifert AH, Krajete A, Bach W, Peckmann J, Paulik C, Firneis MG, Schleper C, Rittmann SKR.	Nat Commun	10.1038/s41467-018-02876-y	2018
Metabolism	Diversity and Niche of Archaea in Bioremediation.	Krzmarzick MJ, Taylor DK, Fu X, McCutchan AL.	Archaea	10.1155/2018/3194108	2018
Metabolism	Assessing the Ecophysiology of Methanogens in the Context of Recent Astrobiological and Planetological Studies.	Taubner RS, Schleper C, Firneis MG, Rittmann SK.	Life (Basel)	10.3390/life5041652	2015
Phylogeny	Comparative genomic analysis reveals starvation survival systems in Methanothermobacter thermautotrophicus DeltaH.	Prathiviraj R, Chellapandi P	Anaerobe	10.1016/j.anaerobe.2020.102216	2020
	Complete Genomic Sequence of the Thermophilic and Hydrogenotrophic Methanogen Methanothermobacter sp. Strain KEPCO-1.	Jeon BS, Kim H, Go YW, Kim YG, Joo JS, Choi O, Sang BI	Microbiol Resour Announc	10.1128/MRA.01034-19	2020
Genetics	The genome of archaeal prophage PsiM100 encodes the lytic enzyme responsible for autolysis of Methanothermobacter wolfeii.	Luo Y, Pfister P, Leisinger T, Wasserfallen A	J Bacteriol	10.1128/JB.183.19.5788-5792.2001	2001
Genetics	Comparative sequence analysis of plasmids pME2001 and pME2200 of methanothermobacter marburgensis strains Marburg and ZH3.	Luo Y, Leisinger T, Wasserfallen A	Plasmid	10.1006/plas.2000.1493	2001
Enzymology	Regulation of the synthesis of H2-forming methylenetetrahydromethanopterin dehydrogenase (Hmd) and of HmdII and HmdIII in Methanothermobacter marburgensis.	Afting C, Kremmer E, Brucker C, Hochheimer A, Thauer RK	Arch Microbiol	10.1007/s002030000197	2000
Phylogeny	Phylogenetic analysis of 18 thermophilic Methanobacterium isolates supports the proposals to create a new genus, Methanothermobacter gen. nov., and to reclassify several isolates in three species, Methanothermobacter thermautotrophicus comb. nov., Methanothermobacter wolfeii comb. nov., and Methanothermobacter marburgensis sp. nov.	Wasserfallen A, Nolling J, Pfister P, Reeve J, Conway de Macario E	Int J Syst Evol Microbiol	10.1099/00207713-50-1-43	2000

References

#972	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2133
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124042	Johannes Wittmann, Clara Rolland, Lorenz Reimer, Joaquim Sardà: PhageDive .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methanothermobacter marburgensis (DSM 2133, NBRC 100331, OCM 82)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Phages

Sequence information

Genome sequences

Genome browser: GCA_000145295

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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