Name: Methanothermobacter defluvii ADZ
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6896

Type strain:

Species: Methanothermobacter defluvii

Strain Designation: ADZ

Culture col. no.: DSM 7466, ATCC 51443, VKM B-1962

Strain history: <- S. V. Kotelnikova and A. Y. Obraztsova; ADZ

NCBI tax ID(s): 49339 (species)

SI-ID 42986

ATCC 51443, DSM 7466, VKM B-1962

Section

Methanothermobacter defluvii ADZ is an anaerobe, thermophilic, Gram-negative archaeon that was isolated from sludge, metacrilic wastes digester.

Gram-negative
anaerobe
thermophilic
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Methanobacteriota*
	Class	*Methanobacteria*
	Order	*Methanobacteriales*
	Family	*Methanobacteriaceae*
	Genus	*Methanothermobacter*
	Species	**Methanothermobacter defluvii**
	Full Scientific Name (LPSN)	Methanothermobacter defluvii (Kotelnikova et al. 1994) Boone 2002

Synonym

Methanobacterium defluvii

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.972

Information on culture and growth conditions Culture and growth conditions

[Ref.: #3171]

Culture medium

METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141)

[Ref.: #3171]

Culture medium growth

[Ref.: #3171]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3171]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #3171]			growth	60 °C

[Ref.: #3171]

Temperature range

thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #3171]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %99.999

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.327

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #3171]	Sample type/isolated from	sludge, metacrilic wastes digester
[Ref.: #3171]	Country	Russia
[Ref.: #3171]	Country ISO 3 Code	RUS
* marker position based on {}

Isolation sources categories

#Engineered	#Biodegradation	-
#Engineered	#Waste	-
#Environmental	#Terrestrial	#Mud (Sludge)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence X99046 (>99% sequence identity) for Methanothermobacter from Microbeatlas

Aquatic Samples	47
Soil Samples	3
Animal Samples	8
Plant Samples	5
Total Samples	63

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #3171]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #3171]	M.defluvii 16S rRNA gene	X99046	1445	ENA	49339 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Methanothermobacter defluvii DSM 7466	GCA_003385755	scaffold	GenBank	49339 tax ID	*
[Ref.: #66792]	Methanothermobacter defluvii DSM 7466	2757320366	draft	JGI IMG/M	49339 tax ID	*
[Ref.: #66792]	Methanothermobacter defluvii strain DSM 7466	49339.3	wgs	PATRIC	49339 tax ID	*

[Ref.: #3171]

GC-content

62.2 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Methanothermobacter defluvii DSM 7466 NCBI: GCA_003385755.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Methanothermobacter defluvii DSM 7466 NCBI: GCA_003385755.1
[Ref.: #69480]	flagellated	no	96.492	no
[Ref.: #69480]	gram-positive	no	97.003	no
[Ref.: #69480]	anaerobic	yes	98.574	no
[Ref.: #69480]	aerobic	no	95.606	yes
[Ref.: #69480]	halophile	no	83.672	no
[Ref.: #69480]	spore-forming	no	96.755	no
[Ref.: #69480]	glucose-util	yes	72.271	no
[Ref.: #69480]	thermophile	yes	88.389	no
[Ref.: #69480]	motile	no	85.52	no
[Ref.: #69480]	glucose-ferment	no	63.681	no

Availability in culture collections External links

[Ref.: #3171]

Culture collection no.

DSM 7466, ATCC 51443, VKM B-1962

[Ref.: #76360]

SI-ID 42986

References

#3171

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 7466

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#76360

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID42986.1 )

* These data were automatically processed and therefore are not curated

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Methanothermobacter defluvii DSM 7466

Methanothermobacter defluvii DSM 7466

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