Name: Methanothermobacter thermautotrophicus Delta H
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6882

Type strain:

Species: Methanothermobacter thermautotrophicus

Strain Designation: Delta H, DeltaH

Culture col. no.: DSM 1053, ATCC 29096, JCM 10044, NBRC 100330, OCM 143, VKM B-1908

Strain history: DSM 1053 <-- J. G. Zeikus ΔH.

NCBI tax ID(s): 145262 (species)

SI-ID 47306

DSM 1053, ATCC 29096, JCM 10044, VKM B-1908, NBRC 100330

Other strains from Methanothermobacter thermautotrophicus

M. thermautotrophicus JW501, DSM 2036
M. thermautotrophicus YTB, DSM 1850
M. thermautotrophicus JW510, DSM 1910
M. thermautotrophicus FTF, DSM 3012
M. thermautotrophicus Z-245, DSM 3720
M. thermautotrophicus Hveragerdi, DSM 3590
M. thermautotrophicus DSM 3848
M. thermautotrophicus SF-4, DSM 6457
M. thermautotrophicus HMT-1, DSM 7077, MCM B 601
M. thermautotrophicus ZH3, DSM 9446

Section

Methanothermobacter thermautotrophicus Delta H is an anaerobe, thermophilic archaeon that was isolated from sewage sludge.

anaerobe
thermophilic
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Methanobacteriota*
	Class	*Methanobacteria*
	Order	*Methanobacteriales*
	Family	*Methanobacteriaceae*
	Genus	*Methanothermobacter*
	Species	**Methanothermobacter thermautotrophicus**
	Full Scientific Name (LPSN)	Methanothermobacter thermautotrophicus corrig. (Zeikus and Wolfe 1972) Wasserfallen et al. 2000

	Synonym	Methanobacterium thermautotrophicum
	Synonym	Methanobacterium thermalcaliphilum
	Synonym	Methanobacterium thermoautotrophicus
	Synonym	Methanobacterium thermoalcaliphilum
	Synonym	Methanobacterium thermoformicicum
	Synonym	Methanobacterium thermoautotrophicum

Information on culture and growth conditions Culture and growth conditions

[Ref.: #387]

Culture medium

MODIFIED METHANOBACTERIUM MEDIUM (DSMZ Medium 1523)

[Ref.: #387]

Culture medium growth

[Ref.: #387]

Culture medium link

Medium recipe at MediaDive

[Ref.: #387]

Culture medium composition

expand / minimize

[Ref.: #387]

Culture medium

METHANOBACTERIUM MEDIUM (DSMZ Medium 119)

[Ref.: #387]

Culture medium growth

[Ref.: #387]

Culture medium link

Medium recipe at MediaDive

[Ref.: #387]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #387]			growth	65 °C
[Ref.: #67770]			growth	65 °C

[Ref.: #387]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #387]

Oxygen tolerance

anaerobe

[Ref.: #387]	Name of produced compound	7,8 didemethyl-8-hydroxy-5-deazariboflavin
[Ref.: #387]	Name of produced compound	coenzyme F₄₂₀

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #387]	Sample type/isolated from	sewage sludge
[Ref.: #387]	Geographic location (country and/or sea, region)	Illinois, Urbana
[Ref.: #387]	Country	USA
[Ref.: #387]	Country ISO 3 Code	USA
[Ref.: #387]	Continent	North America

[Ref.: #67770]	Sample type/isolated from	Anaerobic sewage-sludge
* marker position based on {}

Isolation sources categories

#Engineered

#Waste

#Sewage sludge

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #387]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	M.thermoautotrophicum 5S ribosomal RNA	X62857	123	ENA	145262 tax ID
[Ref.: #67770]	M.thermoautotrophicum (DELTA H) gene for 16S rRNA	X68720	1334	ENA	145262 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Methanothermobacter thermautotrophicus str. Delta H	GCA_000008645	complete	GenBank	187420 tax ID	*
[Ref.: #66792]	Methanothermobacter thermautotrophicus Delta H	638154510	complete	JGI IMG/M	187420 tax ID	*
[Ref.: #66792]	Methanothermobacter thermautotrophicus str. Delta H	187420.15	complete	PATRIC	187420 tax ID	*
[Ref.: #66792]	Methanothermobacter thermautotrophicus Delta H	GCA_027554905	complete	GenBank	145262 tax ID	*

[Ref.: #387]	GC-content	49.7 mol%
[Ref.: #387]	GC-content	48.6 mol%
[Ref.: #67770]	GC-content	52 mol%	Buoyant density centrifugation (BD)
[Ref.: #67770]	GC-content	49.7 mol%	Buoyant density centrifugation (BD)
[Ref.: #67770]	GC-content	50.7 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Methanothermobacter thermautotrophicus str. Delta H Delta H NCBI: GCA_000008645.1
[Ref.: #69480]	motile	no	84.854	no
[Ref.: #69480]	flagellated	no	96.206	no
[Ref.: #69480]	gram-positive	no	97.348	no
[Ref.: #69480]	anaerobic	yes	98.682	yes
[Ref.: #69480]	halophile	no	84.913	no
[Ref.: #69480]	spore-forming	no	96.645	no
[Ref.: #69480]	glucose-util	yes	73.478	no
[Ref.: #69480]	aerobic	no	96.508	no
[Ref.: #69480]	thermophile	yes	90.251	yes
[Ref.: #69480]	glucose-ferment	no	63.601	no

Availability in culture collections External links

[Ref.: #387]

Culture collection no.

DSM 1053, ATCC 29096, JCM 10044, NBRC 100330, OCM 143, VKM B-1908

[Ref.: #76346]

SI-ID 47306

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Metabolism	A Shuttle-Vector System Allows Heterologous Gene Expression in the Thermophilic Methanogen Methanothermobacter thermautotrophicus DeltaH.	Fink C, Beblawy S, Enkerlin AM, Muhling L, Angenent LT, Molitor B	mBio	10.1128/mBio.02766-21	2021	*
Phylogeny	Comparative genomic analysis reveals starvation survival systems in Methanothermobacter thermautotrophicus DeltaH.	Prathiviraj R, Chellapandi P	Anaerobe	10.1016/j.anaerobe.2020.102216	2020	*
Phylogeny	Zhaonella formicivorans gen. nov., sp. nov., an anaerobic formate-utilizing bacterium isolated from Shengli oilfield, and proposal of four novel families and Moorellales ord. nov. in the phylum Firmicutes.	Lv XM, Yang M, Dai LR, Tu B, Chang C, Huang Y, Deng Y, Lawson PA, Zhang H, Cheng L, Tang YQ	Int J Syst Evol Microbiol	10.1099/ijsem.0.004178	2020	*
Metabolism	Functional annotation of operome from Methanothermobacter thermautotrophicus DeltaH: An insight to metabolic gap filling.	Prathiviraj R, Chellapandi P	Int J Biol Macromol	10.1016/j.ijbiomac.2018.11.100	2018	*
Genetics	Intergenomic evolution and metabolic cross-talk between rumen and thermophilic autotrophic methanogenic archaea.	Bharathi M, Chellapandi P	Mol Phylogenet Evol	10.1016/j.ympev.2016.11.008	2016	*
Metabolism	Physiological and genetic basis for self-aggregation of a thermophilic hydrogenotrophic methanogen, Methanothermobacter strain CaT2.	Kosaka T, Toh H, Fujiyama A, Sakaki Y, Watanabe K, Meng XY, Hanada S, Toyoda A	Environ Microbiol Rep	10.1111/1758-2229.12128	2013	*
Stress	Without salt, the 'thermophilic' protein Mth10b is just mesophilic.	Zhang N, Pan XM, Ge M	PLoS One	10.1371/journal.pone.0053125	2012	*
Metabolism	Acceleration of cellulose degradation and shift of product via methanogenic co-culture of a cellulolytic bacterium with a hydrogenotrophic methanogen.	Sasaki D, Morita M, Sasaki K, Watanabe A, Ohmura N	J Biosci Bioeng	10.1016/j.jbiosc.2012.05.002	2012	*
Phylogeny	Methanothermobacter tenebrarum sp. nov., a hydrogenotrophic, thermophilic methanogen isolated from gas-associated formation water of a natural gas field.	Nakamura K, Takahashi A, Mori C, Tamaki H, Mochimaru H, Nakamura K, Takamizawa K, Kamagata Y	Int J Syst Evol Microbiol	10.1099/ijs.0.041681-0	2012	*
Metabolism	Molecular mechanism underlying the interaction of typical Sac10b family proteins with DNA.	Liu YF, Zhang N, Liu X, Wang X, Wang ZX, Chen Y, Yao HW, Ge M, Pan XM	PLoS One	10.1371/journal.pone.0034986	2012	*
Metabolism	Comparative proteomic analysis of Methanothermobacter themautotrophicus DeltaH in pure culture and in co-culture with a butyrate-oxidizing bacterium.	Enoki M, Shinzato N, Sato H, Nakamura K, Kamagata Y	PLoS One	10.1371/journal.pone.0024309	2011	*
Phylogeny	Isolation and characterization of Methanothermobacter crinale sp. nov., a novel hydrogenotrophic methanogen from the Shengli oil field.	Cheng L, Dai L, Li X, Zhang H, Lu Y	Appl Environ Microbiol	10.1128/AEM.00210-11	2011	*
Metabolism	Mth10b, a unique member of the Sac10b family, does not bind nucleic acid.	Liu YF, Zhang N, Yao HW, Pan XM, Ge M	PLoS One	10.1371/journal.pone.0019977	2011	*
Phylogeny	Thermosyntropha tengcongensis sp. nov., a thermophilic bacterium that degrades long-chain fatty acids syntrophically.	Zhang F, Liu X, Dong X	Int J Syst Evol Microbiol	10.1099/ijs.0.033456-0	2011	*
Metabolism	Crystal structure analysis of DNA uridine endonuclease Mth212 bound to DNA.	Lakomek K, Dickmanns A, Ciirdaeva E, Schomacher L, Ficner R	J Mol Biol	10.1016/j.jmb.2010.04.044	2010	*
Metabolism	Archaeal DNA uracil repair via direct strand incision: A minimal system reconstituted from purified components.	Schomacher L, Schurer KA, Ciirdaeva E, McDermott P, Chong JP, Kramer W, Fritz HJ	DNA Repair (Amst)	10.1016/j.dnarep.2010.01.004	2010	*
Metabolism	An examination of the relationship between active site loop size and thermodynamic activation parameters for orotidine 5'-monophosphate decarboxylase from mesophilic and thermophilic organisms.	Toth K, Amyes TL, Wood BM, Chan KK, Gerlt JA, Richard JP	Biochemistry	10.1021/bi901064k	2009	*
Metabolism	DNA uracil repair initiated by the archaeal ExoIII homologue Mth212 via direct strand incision.	Schomacher L, Chong JP, McDermott P, Kramer W, Fritz HJ	Nucleic Acids Res	10.1093/nar/gkp102	2009	*
Metabolism	Functional characterization of the DnaK chaperone system from the archaeon Methanothermobacter thermautotrophicus DeltaH.	Popp SL, Reinstein J	FEBS Lett	10.1016/j.febslet.2008.12.062	2009	*
Phylogeny	Thermodesulfovibrio aggregans sp. nov. and Thermodesulfovibrio thiophilus sp. nov., anaerobic, thermophilic, sulfate-reducing bacteria isolated from thermophilic methanogenic sludge, and emended description of the genus Thermodesulfovibrio.	Sekiguchi Y, Muramatsu M, Imachi H, Narihiro T, Ohashi A, Harada H, Hanada S, Kamagata Y	Int J Syst Evol Microbiol	10.1099/ijs.0.2008/000893-0	2008	*
Enzymology	Specific DNA binding of a potential transcriptional regulator, inosine 5'-monophosphate dehydrogenase-related protein VII, to the promoter region of a methyl coenzyme m reductase I-encoding operon retrieved from Methanothermobacter thermautotrophicus strain DeltaH.	Shinzato N, Enoki M, Sato H, Nakamura K, Matsui T, Kamagata Y	Appl Environ Microbiol	10.1128/AEM.02155-07	2008	*
Metabolism	Comparative transcriptome analysis of responses of Methanothermobacter thermautotrophicus to different environmental stimuli.	Kato S, Kosaka T, Watanabe K	Environ Microbiol	10.1111/j.1462-2920.2007.01508.x	2007	*
Cultivation	Methanogenesis is Ca2+ dependent in Methanothermobacter thermautotrophicus strain DeltaH.	Vancek M, Vidova M, Majernik AI, Smigan P	FEMS Microbiol Lett	10.1111/j.1574-6968.2006.00232.x	2006	*
Metabolism	Coaggregation facilitates interspecies hydrogen transfer between Pelotomaculum thermopropionicum and Methanothermobacter thermautotrophicus.	Ishii S, Kosaka T, Hori K, Hotta Y, Watanabe K	Appl Environ Microbiol	10.1128/AEM.71.12.7838-7845.2005	2005	*
Phylogeny	Improved strategy for presumptive identification of methanogens using 16S riboprinting.	Wright AD, Pimm C	J Microbiol Methods	10.1016/s0167-7012(03)00169-6	2003	*
Metabolism	The cobY gene of the archaeon Halobacterium sp. strain NRC-1 is required for de novo cobamide synthesis.	Woodson JD, Peck RF, Krebs MP, Escalante-Semerena JC	J Bacteriol	10.1128/JB.185.1.311-316.2003	2003	*
Metabolism	Differential expression of methanogenesis genes of Methanothermobacter thermoautotrophicus (formerly Methanobacterium thermoautotrophicum) in pure culture and in cocultures with fatty acid-oxidizing syntrophs.	Luo HW, Zhang H, Suzuki T, Hattori S, Kamagata Y	Appl Environ Microbiol	10.1128/AEM.68.3.1173-1179.2002	2002	*
Metabolism	The zinc finger domain of the archaeal minichromosome maintenance protein is required for helicase activity.	Poplawski A, Grabowski B, Long SE, Kelman Z	J Biol Chem	10.1074/jbc.M108519200	2001	*
Metabolism	Convenient fluorescence-based methods to measure membrane potential and intracellular pH in the Archaeon Methanobacterium thermoautotrophicum.	de Poorter LM, Keltjens JT	J Microbiol Methods	10.1016/s0167-7012(01)00312-8	2001	*
Metabolism	Crystal structure of the MJ0490 gene product of the hyperthermophilic archaebacterium Methanococcus jannaschii, a novel member of the lactate/malate family of dehydrogenases.	Lee BI, Chang C, Cho SJ, Eom SH, Kim KK, Yu YG, Suh SW	J Mol Biol	10.1006/jmbi.2001.4532	2001	*
Metabolism	A DNA ligase from a hyperthermophilic archaeon with unique cofactor specificity.	Nakatani M, Ezaki S, Atomi H, Imanaka T	J Bacteriol	10.1128/JB.182.22.6424-6433.2000	2000	*
Enzymology	Identification of an alternative nucleoside triphosphate: 5'-deoxyadenosylcobinamide phosphate nucleotidyltransferase in Methanobacterium thermoautotrophicum delta H.	Thomas MG, Escalante-Semerena JC	J Bacteriol	10.1128/JB.182.15.4227-4233.2000	2000	*
Genetics	A unique organization of the protein subunits of the DNA polymerase clamp loader in the archaeon Methanobacterium thermoautotrophicum deltaH.	Kelman Z, Hurwitz J	J Biol Chem	10.1074/jbc.275.10.7327	2000	*
Metabolism	The single minichromosome maintenance protein of Methanobacterium thermoautotrophicum DeltaH contains DNA helicase activity.	Kelman Z, Lee JK, Hurwitz J	Proc Natl Acad Sci U S A	10.1073/pnas.96.26.14783	1999	*
Enzymology	A plant-type (beta-class) carbonic anhydrase in the thermophilic methanoarchaeon Methanobacterium thermoautotrophicum.	Smith KS, Ferry JG	J Bacteriol	10.1128/JB.181.20.6247-6253.1999	1999	*
Enzymology	Isolation and characterization of a split B-type DNA polymerase from the archaeon Methanobacterium thermoautotrophicum deltaH.	Kelman Z, Pietrokovski S, Hurwitz J	J Biol Chem	10.1074/jbc.274.40.28751	1999	*
Metabolism	Methanobacterium thermoautotrophicum RNA polymerase and transcription in vitro.	Darcy TJ, Hausner W, Awery DE, Edwards AM, Thomm M, Reeve JN	J Bacteriol	10.1128/JB.181.14.4424-4429.1999	1999	*
Enzymology	Two malate dehydrogenases in Methanobacterium thermoautotrophicum.	Thompson H, Tersteegen A, Thauer RK, Hedderich R	Arch Microbiol	10.1007/s002030050612	1998	*
Phylogeny	Isolation and characterization of Methanobacterium thermoautotrophicum DeltaH mutants unable to grow under hydrogen-deprived conditions.	Pennings JL, Keltjens JT, Vogels GD	J Bacteriol	10.1128/JB.180.10.2676-2681.1998	1998	*
Metabolism	Methanopterin biosynthesis: methylation of the biosynthetic intermediates.	White RH	Biochim Biophys Acta	10.1016/s0304-4165(97)00148-7	1998	*
Enzymology	Purification, regulation, and molecular and biochemical characterization of pyruvate carboxylase from Methanobacterium thermoautotrophicum strain deltaH.	Mukhopadhyay B, Stoddard SF, Wolfe RS	J Biol Chem	10.1074/jbc.273.9.5155	1998	*
Phylogeny	DNA relatedness among some thermophilic members of the genus Methanobacterium: emendation of the species Methanobacterium thermoautotrophicum and rejection of Methanobacterium thermoformicicum as a synonym of Methanobacterium thermoautotrophicum.	Touzel JP, Conway de Macario E, Nolling J, De Vos WM, Zhilina T, Lysenko AM	Int J Syst Bacteriol	10.1099/00207713-42-3-408	1992	*
Metabolism	Diversity and population dynamics of methanogenic bacteria in a granular consortium.	Visser FA, van Lier JB, Macario AJ, Conway de Macario E	Appl Environ Microbiol	10.1128/aem.57.6.1728-1734.1991	1991	*
Biotechnology	Production of ethane, ethylene, and acetylene from halogenated hydrocarbons by methanogenic bacteria.	Belay N, Daniels L	Appl Environ Microbiol	10.1128/aem.53.7.1604-1610.1987	1987	*
Enzymology	A plasmid in the archaebacterium Methanobacterium thermoautotrophicum.	Meile L, Kiener A, Leisinger T	Mol Gen Genet	10.1007/BF00425766	1983	*
Enzymology	Paramagnetic centers in the nickel-containing, deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum.	Kojima N, Fox JA, Hausinger RP, Daniels L, Orme-Johnson WH, Walsh C	Proc Natl Acad Sci U S A	10.1073/pnas.80.2.378	1983	*
Enzymology	Microbiology of methanogenesis in thermal, volcanic environments.	Zeikus JG, Ben-Bassat A, Hegge PW	J Bacteriol	10.1128/jb.143.1.432-440.1980	1980	*
Metabolism	Functional Characterization and Screening of Promiscuous Kinases and Isopentenyl Phosphate Kinases for the Synthesis of DMAPP via a One-Pot Enzymatic Cascade.	Qiu C, Liu Y, Wu Y, Zhao L, Pei J	Int J Mol Sci	10.3390/ijms232112904	2022	*
Pathogenicity	An Interdomain Conjugation Protocol for Plasmid-DNA Transfer into Methanothermobacter thermautotrophicus DeltaH.	Fink C, Angenent LT, Molitor B	Methods Mol Biol	10.1007/978-1-0716-2445-6_7	2022	*
	Methanothermobacter thermautotrophicus modulates its membrane lipids in response to hydrogen and nutrient availability.	Yoshinaga MY, Gagen EJ, Wormer L, Broda NK, Meador TB, Wendt J, Thomm M, Hinrichs KU	Front Microbiol	10.3389/fmicb.2015.00005	2015	*
	Biochemical analysis of neomycin-resistance in the methanoarchaeon Methanothermobacter thermautotrophicus and some implications for energetic processes in this strain.	Majernik A, Cubonova L, Polak P, Smigan P, Greksak M	Anaerobe	10.1016/S1075-9964(03)00042-8	2003	*
	Identification, Biosynthesis, and Function of 1,3,4,6-Hexanetetracarboxylic Acid in Methanobacterium thermoautotrophicum DeltaH.	Gorkovenko A, Roberts MF, White RH	Appl Environ Microbiol	10.1128/aem.60.4.1249-1253.1994	1994	*
	Enrichment of Thermophilic Propionate-Oxidizing Bacteria in Syntrophy with Methanobacterium thermoautotrophicum or Methanobacterium thermoformicicum.	Stams AJ, Grolle KC, Frijters CT, Van Lier JB	Appl Environ Microbiol	10.1128/aem.58.1.346-352.1992	1992	*
	Hydrogen partial pressures in a thermophilic acetate-oxidizing methanogenic coculture.	Lee MJ, Zinder SH	Appl Environ Microbiol	10.1128/aem.54.6.1457-1461.1988	1988	*
	Comparison of assimilatory organic nitrogen, sulfur, and carbon sources for growth of methanobacterium species.	Bhatnagar L, Jain MK, Aubert JP, Zeikus JG	Appl Environ Microbiol	10.1128/aem.48.4.785-790.1984	1984	*

References

#387	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 1053

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#76346

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID47306.1 )

* These data were automatically processed and therefore are not curated

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Methanothermobacter thermautotrophicus str. Delta H Delta H

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