Name: Listeria monocytogenes 1224.72
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 6873

Type strain:

Species: Listeria monocytogenes

Strain Designation: 1224.72

Culture col. no.: DSM 15675, NCTC 11994, WDCM 00019, CIP 110274, CECT 4032, LMG 13305

Strain history: CIP <- 2011, DSMZ <- NCTC <- P.N. Pini, CPHL, Colindale, UK

NCBI tax ID(s): 1639 (species)

SI-ID 14378

LMG 13305, NCTC 11994, CECT 4032, DSM 15675, BCRC 15408, CCRC 15408, KACC 10550, ACM 4986, CCMM B397, CIP 110274

Special Collections

DSMZ
CIP

Other strains from Listeria monocytogenes

Section

Listeria monocytogenes 1224.72 is a facultative anaerobe, Gram-positive bacterium that was isolated from soft cheese.

Gram-positive
facultative anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Listeriaceae*
	Genus	*Listeria*
	Species	**Listeria monocytogenes**
	Full Scientific Name (LPSN)	Listeria monocytogenes (Murray et al. 1926) Pirie 1940 (Approved Lists 1980)

Synonym

"Bacterium monocytogenes"

Information on morphological and physiological properties Morphology

[Ref.: #121008]	Gram stain	positive

[Ref.: #121008]	Cell shape	rod-shaped

[Ref.: #121008]	Motility	no

[Ref.: #6028]	Type of hemolysis	gamma
[Ref.: #6028]	Incubation period	1-2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6028]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #6028]

Culture medium growth

[Ref.: #6028]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6028]

Culture medium composition

expand / minimize

[Ref.: #6028]

Culture medium

BHI MEDIUM (DSMZ Medium 215)

[Ref.: #6028]

Culture medium growth

[Ref.: #6028]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6028]

Culture medium composition

expand / minimize

[Ref.: #41844]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #41844]

Culture medium growth

[Ref.: #41844]

Culture medium composition

expand / minimize

[Ref.: #121008]

Culture medium

CIP Medium 338

[Ref.: #121008]

Culture medium growth

[Ref.: #121008]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #121008]

Culture medium

CIP Medium 72

[Ref.: #121008]

Culture medium growth

[Ref.: #121008]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #6028]			growth	37 °C
[Ref.: #41844]			growth	37 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #121008]

Oxygen tolerance

facultative anaerobe

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68376]	D-arabitol ChEBI	+	builds acid from	* from API LIST
[Ref.: #68376]	D-ribose ChEBI	-	builds acid from	* from API LIST
[Ref.: #68376]	D-tagatose ChEBI	-	builds acid from	* from API LIST
[Ref.: #68376]	esculin ChEBI	+	hydrolysis	* from API LIST
[Ref.: #68376]	glucose 1-phosphate ChEBI	-	builds acid from	* from API LIST
[Ref.: #68376]	L-rhamnose ChEBI	+	builds acid from	* from API LIST
[Ref.: #68376]	methyl alpha-D-glucopyranoside ChEBI	+	builds acid from	* from API LIST
[Ref.: #121008]	nitrate ChEBI	-	reduction
[Ref.: #121008]	nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #121008]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	+	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym, API LIST
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #6028]	catalase	+	1.11.1.6
[Ref.: #121008]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #6028]	cytochrome-c oxidase	-	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #121008]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #121008]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® LIST:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Differentiation L. innocura(+)/ L.monoytogenes(-)DIM	Esculin hydrolysisESC	alpha-Mannosidasealpha MAN	Acid from D-arabitolDARL	Acid from D-xyloseXYL	Acid from L-rhamnoseRHA	Acid from methyl-alpha-D-glucopyranosideMDG	Acid from D-riboseRIB	Acid from alpha-D-glucose 1-phosphateG1P	Acid from D-tagatoseTAG	beta hemolysis presentbeta HEM
[Ref.: #6028]	1129	+	+	+	+	-	+	+	-	-	-	+
[Ref.: #6028]	1118	-	+	+	+	-	+	+	-	-	-	+
[Ref.: #6028]	1105	-	+	+	+	+/-	+	+	-	-	-	not determinedn.d.
[Ref.: #6028]	1048	-	+	-	+	-	+	+	-	-	-	not determinedn.d.
[Ref.: #6028]	1047	+/-	+	+	+	+/-	+	+	-	-	-	-
[Ref.: #6028]	1046	+/-	+	+	+	+/-	+	+	-	-	-	-
[Ref.: #6028]	1045	+/-	+	+	+	-	+	+	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121008]	12480	-	-	+	-	-	-	-	-	-	-	+	+	-	-	-	-	+	+	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6028]	Sample type/isolated from	soft cheese

[Ref.: #121008]	Sample type/isolated from	Food, Soft cheese
* marker position based on {}

Isolation sources categories

#Engineered

#Food production

#Dairy product

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6028]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #121008]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #124043]	Listeria monocytogenes partial 16S rRNA gene, strain CECT 4032	AJ508749	1068	GenBank ENA	1639 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Listeria monocytogenes NCTC11994	GCA_900452915	contig	GenBank ENA	1639 tax ID	*
[Ref.: #66792]	Listeria monocytogenes serotype 4b str. NCTC 11994	GCA_002156185	contig	GenBank ENA	1344576 tax ID	*
[Ref.: #66792]	Listeria monocytogenes serotype 4b str. NCTC 11994 strain NCTC 11994	1344576.3	wgs	PATRIC	1344576 tax ID	*
[Ref.: #66792]	Listeria monocytogenes strain NCTC11994	1639.4971	wgs	PATRIC	1639 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Listeria monocytogenes strain NCTC11994 PATRIC: 1639.4971
[Ref.: #69481]	spore-forming	spore-formingⓘ	yes	59	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Listeria monocytogenes serotype 4b str. NCTC 11994 strain NCTC 11994 PATRIC: 1344576.3
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	82.305	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	91.058	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	50.318	no
[Ref.: #69480]	aerobic	aerobicⓘ	no	87.235	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	95.902	no
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	65.516	no

Availability in culture collections External links

[Ref.: #6028]

Culture collection no.

DSM 15675, NCTC 11994, WDCM 00019, CIP 110274, CECT 4032, LMG 13305

[Ref.: #76338]

SI-ID 14378

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	International interlaboratory study comparing single organism 16S rRNA gene sequencing data: Beyond consensus sequence comparisons.	Olson ND, Lund SP, Zook JM, Rojas-Cornejo F, Beck B, Foy C, Huggett J, Whale AS, Sui Z, Baoutina A, Dobeson M, Partis L, Morrow JB	Biomol Detect Quantif	10.1016/j.bdq.2015.01.004	2015	*
Biotechnology	Behavior of Listeria monocytogenes in Sliced Ready-to-Eat Meat Products Packaged under Vacuum or Modified Atmosphere Conditions.	Menendez RA, Rendueles E, Sanz JJ, Capita R, Garcia-Fernandez C	J Food Prot	10.4315/0362-028X.JFP-15-103	2015	*
Metabolism	Bacterial inactivation by high-voltage atmospheric cold plasma: influence of process parameters and effects on cell leakage and DNA.	Han L, Patil S, Keener KM, Cullen PJ, Bourke P	J Appl Microbiol	10.1111/jam.12426	2014	*
Pathogenicity	Combined effect of sodium nitrite with high-pressure treatments on the inactivation of Escherichia coli BW25113 and Listeria monocytogenes NCTC 11994.	De Alba M, Bravo D, Medina M, Park SF, Mackey BM	Lett Appl Microbiol	10.1111/lam.12031	2013	*
Biotechnology	Effects of vegetable type, package atmosphere and storage temperature on growth and survival of Escherichia coli O157:H7 and Listeria monocytogenes.	Francis GA, O'Beirne D	J Ind Microbiol Biotechnol	10.1038/sj.jim.7000094	2001	*
Pathogenicity	Inactivation and injury of pressure-resistant strains of Escherichia coli O157 and Listeria monocytogenes in fruit juices.	Jordan SL, Pascual C, Bracey E, Mackey BM	J Appl Microbiol	10.1046/j.1365-2672.2001.01402.x	2001	*
Biotechnology	Mechanism of action of pulsed high electric field (PHEF) on the membranes of food-poisoning bacteria is an 'all-or-nothing' effect.	Russell NJ, Colley M, Simpson RK, Trivett AJ, Evans RI	Int J Food Microbiol	10.1016/s0168-1605(00)00169-0	2000	*
Enzymology	Pulsed high electric field causes 'all or nothing' membrane damage in Listeria monocytogenes and Salmonella typhimurium, but membrane H+-ATPase is not a primary target.	Simpson RK, Whittington R, Earnshaw RG, Russell NJ	Int J Food Microbiol	10.1016/s0168-1605(99)00022-7	1999	*
Pathogenicity	Cytotoxic T-cell, delayed type hypersensitive and listeriolysin O responses in experimental bovine listeriosis.	Barbuddhe SB, Malik SV, Bhatnagar S, Gupta LK	Vet Microbiol	10.1016/s0378-1135(98)00243-0	1999	*
Enzymology	Polymorphism of Listeria monocytogenes and Listeria innocua strains isolated from short-ripened cheeses.	Margolles A, Mayo B, de los Reyes-Gavilan CG	J Appl Microbiol	10.1046/j.1365-2672.1998.00339.x	1998	*
Enzymology	The effect of high hydrostatic pressure on the activity of intracellular enzymes of Listeria monocytogenes.	Simpson RK, Gilmour A	Lett Appl Microbiol	10.1046/j.1472-765x.1997.00168.x	1997	*
	Influence of oxygen exclusion and temperature on pathogenic bacteria levels and sensory characteristics of packed ostrich steaks throughout refrigerated storage.	Gonzalez-Montalvo B, Capita R, Guevara-Franco JA, Prieto M, Alonso-Calleja C	Meat Sci	10.1016/j.meatsci.2006.10.025	2006	*
	Reduction of counts of Listeria monocytogenes in cheese by means of high hydrostatic pressure.	Lopez-Pedemonte T, Roig-Sagues A, De Lamo S, Hernandez-Herrero M, Guamis B	Food Microbiol	10.1016/j.fm.2006.03.008	2006	*
	[Effect of high hydrostatic pressure on microbial physiological characteristics].	Li ZJ, Xu JX	Wei Sheng Wu Xue Bao		2005	*
	Sensitivity of Vegetative Pathogens to High Hydrostatic Pressure Treatment in Phosphate-Buffered Saline and Foods.	Patterson MF, Quinn M, Simpson R, Gilmour A	J Food Prot	10.4315/0362-028X-58.5.524	1995	*

References

#6028

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15675

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#41844 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68376

Automatically annotated from API LIST .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#76338

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID14378.1 )

#121008 Collection of Institut Pasteur ; Curators of the CIP; CIP 110274

#124043

Dr. Isabel Schober, Dr. Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .

* These data were automatically processed and therefore are not curated

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Listeria monocytogenes strain NCTC11994

Listeria monocytogenes serotype 4b str. NCTC 11994 strain NCTC 11994

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