Brochothrix thermosphacta (DSM 20599, G 14)

Name: Brochothrix thermosphacta (DSM 20599, G 14)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6868

Type strain

Strain Designation G 14

Culture col. no. DSM 20599 G 14

NCBI tax ID(s) 2756

Special Collections DSMZ

Links

Brochothrix thermosphacta G 14 is a mesophilic prokaryote that was isolated from bacon.

mesophilic 16S sequence

Name and taxonomic classification

SI-ID 49654

DSM 20599

@ref 20215

Last LPSN update 2025-12-16 09:48:26

Domain Bacillati

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Listeriaceae

Genus Brochothrix

Species Brochothrix thermosphacta

Full scientific name Brochothrix thermosphacta (McLean and Sulzbacher 1953) Sneath and Jones 1976 (Approved Lists 1980)

Synonyms (1)

"Microbacterium thermosphactum"

BacDive ID	Other strains from **Brochothrix thermosphacta** (12)	Type strain
6867	B. thermosphacta SW 26, DSM 20171, ATCC 11509, NCDO 1676, ... (type strain)
134809	B. thermosphacta B5, CIP 69.6
134810	B. thermosphacta 12, CIP 69.7
138502	B. thermosphacta SW4, CIP 69.8
138503	B. thermosphacta 34, CIP 69.9
138504	B. thermosphacta SW5, CIP 69.10
138624	B. thermosphacta 844-84, CIP 101246, ATCC 51924
138935	B. thermosphacta 19, CIP 69.11
148228	B. thermosphacta CCUG 35074
152051	B. thermosphacta CCUG 45641
156210	B. thermosphacta CCUG 60737
174183	B. thermosphacta 160x8, CIP 110942

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
8947	CORYNEBACTERIUM AGAR (DSMZ Medium 53)		Medium recipe at MediaDive	Name: CORYNEBACTERIUM AGAR (DSMZ Medium 53) Composition: Agar 15.0 g/l Casein peptone 10.0 g/l NaCl 5.0 g/l Glucose 5.0 g/l Yeast extract 5.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
8947	positive	growth	30	mesophilic

Physiology and metabolism

Murein

@ref	Murein short key	Type
8947	A31	A1gamma m-Dpm-direct

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	teichoic acid biosynthesis	100	1 of 1
66794	aspartate and asparagine metabolism	100	9 of 9
66794	ethanol fermentation	100	2 of 2
66794	acetate fermentation	100	4 of 4
66794	coenzyme A metabolism	100	4 of 4
66794	vitamin K metabolism	100	5 of 5
66794	acetoin degradation	100	3 of 3
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	folate polyglutamylation	100	1 of 1
66794	ppGpp biosynthesis	100	4 of 4
66794	palmitate biosynthesis	95.45	21 of 22
66794	threonine metabolism	90	9 of 10
66794	chorismate metabolism	88.89	8 of 9
66794	valine metabolism	88.89	8 of 9
66794	vitamin B1 metabolism	84.62	11 of 13
66794	pentose phosphate pathway	81.82	9 of 11
66794	metabolism of amino sugars and derivatives	80	4 of 5
66794	peptidoglycan biosynthesis	80	12 of 15
66794	photosynthesis	78.57	11 of 14
66794	phenylalanine metabolism	76.92	10 of 13
66794	isoleucine metabolism	75	6 of 8
66794	C4 and CAM-carbon fixation	75	6 of 8
66794	flavin biosynthesis	73.33	11 of 15
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	pyrimidine metabolism	71.11	32 of 45
66794	glycolysis	70.59	12 of 17
66794	purine metabolism	70.21	66 of 94
66794	myo-inositol biosynthesis	70	7 of 10
66794	starch degradation	70	7 of 10
66794	Entner Doudoroff pathway	70	7 of 10
66794	degradation of sugar alcohols	68.75	11 of 16
66794	serine metabolism	66.67	6 of 9
66794	enterobactin biosynthesis	66.67	2 of 3
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	glycolate and glyoxylate degradation	66.67	4 of 6
66794	d-mannose degradation	66.67	6 of 9
66794	formaldehyde oxidation	66.67	2 of 3
66794	NAD metabolism	66.67	12 of 18
66794	alanine metabolism	65.52	19 of 29
66794	methionine metabolism	65.38	17 of 26
66794	non-pathway related	63.16	24 of 38
66794	ketogluconate metabolism	62.5	5 of 8
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
66794	glutamate and glutamine metabolism	60.71	17 of 28
66794	oxidative phosphorylation	60.44	55 of 91
66794	glycogen metabolism	60	3 of 5
66794	3-chlorocatechol degradation	60	3 of 5
66794	methylglyoxal degradation	60	3 of 5
66794	isoprenoid biosynthesis	57.69	15 of 26
66794	tetrahydrofolate metabolism	57.14	8 of 14
66794	propanol degradation	57.14	4 of 7
66794	heme metabolism	57.14	8 of 14
66794	CO2 fixation in Crenarchaeota	55.56	5 of 9
66794	d-xylose degradation	54.55	6 of 11
66794	proline metabolism	54.55	6 of 11
66794	leucine metabolism	53.85	7 of 13
66794	histidine metabolism	51.72	15 of 29
66794	glycogen biosynthesis	50	2 of 4
66794	CMP-KDO biosynthesis	50	2 of 4
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	tryptophan metabolism	50	19 of 38
66794	suberin monomers biosynthesis	50	1 of 2
66794	dolichol and dolichyl phosphate biosynthesis	50	1 of 2
66794	sulfopterin metabolism	50	2 of 4
66794	glutathione metabolism	50	7 of 14
66794	cysteine metabolism	50	9 of 18
66794	arginine metabolism	50	12 of 24
66794	quinate degradation	50	1 of 2
66794	citric acid cycle	50	7 of 14
66794	butanoate fermentation	50	2 of 4
66794	cis-vaccenate biosynthesis	50	1 of 2
66794	gluconeogenesis	50	4 of 8
66794	selenocysteine biosynthesis	50	3 of 6
66794	urea cycle	46.15	6 of 13
66794	phenylpropanoid biosynthesis	46.15	6 of 13
66794	vitamin B6 metabolism	45.45	5 of 11
66794	lipid metabolism	45.16	14 of 31
66794	lysine metabolism	42.86	18 of 42
66794	tyrosine metabolism	42.86	6 of 14
66794	reductive acetyl coenzyme A pathway	42.86	3 of 7
66794	degradation of pentoses	42.86	12 of 28
66794	mevalonate metabolism	42.86	3 of 7
66794	ubiquinone biosynthesis	42.86	3 of 7
66794	lipoate biosynthesis	40	2 of 5
66794	propionate fermentation	40	4 of 10
66794	glycine metabolism	40	4 of 10
66794	cellulose degradation	40	2 of 5
66794	dTDPLrhamnose biosynthesis	37.5	3 of 8
66794	metabolism of disaccharids	36.36	4 of 11
66794	lipid A biosynthesis	33.33	3 of 9
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	cyanate degradation	33.33	1 of 3
66794	degradation of aromatic, nitrogen containing compounds	33.33	4 of 12
66794	IAA biosynthesis	33.33	1 of 3
66794	octane oxidation	33.33	1 of 3
66794	4-hydroxymandelate degradation	33.33	3 of 9
66794	degradation of hexoses	33.33	6 of 18
66794	sphingosine metabolism	33.33	2 of 6
66794	sulfate reduction	30.77	4 of 13
66794	coenzyme M biosynthesis	30	3 of 10
66794	ascorbate metabolism	27.27	6 of 22
66794	biotin biosynthesis	25	1 of 4
66794	carnitine metabolism	25	2 of 8
66794	lactate fermentation	25	1 of 4
66794	cyclohexanol degradation	25	1 of 4
66794	vitamin B12 metabolism	23.53	8 of 34
66794	phosphatidylethanolamine bioynthesis	23.08	3 of 13

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Food production	#Meat

Isolation

8947

Sample typebacon

Taxonmaps

69479

Global distribution of 16S sequence AY543024 (>99% sequence identity) for Brochothrix from Microbeatlas

Aquatic Samples	5597
Soil Samples	3704
Animal Samples	29797
Plant Samples	1458
Total Samples	40556

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
8947	1	Risk group (German classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Brochothrix thermosphacta isolate DSMZ 20599 16S ribosomal RNA gene, partial sequence	AY543024	1420		2756

Genome-based predictions

Literature

References

#8947	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20599
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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