Brochothrix campestris (DSM 4712, ATCC 43754, CIP 102920)

Name: Brochothrix campestris (DSM 4712, ATCC 43754, CIP 102920)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6866

Type strain

Strain Designation S3, S₃

Culture col. no. DSM 4712 ATCC 43754 CIP 102920 S3 S₃

NCBI tax ID(s) 2757

Special Collections DSMZ CIP

History DSMZ <- CIP <- Talon et al., S₃ CIP <- 1987, R. Talon: strain S3 CIP <- 1987, R. Talon, INRA, Ceyrat, France: strain S3

Links

Brochothrix campestris S3 is a facultative anaerobe, Gram-positive, rod-shaped bacterium that was isolated from soil.

Gram-positive rod-shaped facultative anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 92132

ATCC 43754,CIP 102920,DSM 4712,NCFB2833,NCIMB 12903,CCM 4218,IFO 15547,KCTC 3520,NBRC 15547

@ref 20215

Last LPSN update 2026-05-29 08:26:30

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Listeriaceae

Genus Brochothrix

Species Brochothrix campestris

Full scientific name Brochothrix campestris Talon et al. 1988

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
117114	positive	rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
1727	TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)	Medium recipe at MediaDive	Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
41201	MEDIUM 3 - Columbia agar		Columbia agar (39.000 g);distilled water (1000.000 ml)
117114	CIP Medium 3	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
1727	positive	growth	26
41201	positive	growth	22
117114	positive	growth	15-37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
117114	facultative anaerobe
125439	facultative anaerobe	93.733

Halophily

@ref	Salt	Growth	Tested relation	Concentration
117114	NaCl	positive	growth	0-4 %
117114	NaCl		growth	6 %
117114	NaCl		growth	8 %
117114	NaCl		growth	10 %

Murein

@ref	Murein short key	Type
1727	A31	A1gamma m-Dpm-direct

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	+	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	+	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	+	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	+	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	+	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	+	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	+	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
117114	606565 ChEBI	hippurate	+	hydrolysis
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	+	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
117114	17632 ChEBI	nitrate	-	reduction
117114	17632 ChEBI	nitrate	+	respiration
117114	16301 ChEBI	nitrite	-	reduction
68371	0 ChEBI	Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371	0 ChEBI	Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	+	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	+	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	+	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite	Production
117114	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
117114	15688 ChEBI	acetoin	+
117114	17234 ChEBI	glucose		+

Enzymes

@ref	Value	Activity	Ec
117114	alcohol dehydrogenase	-	1.1.1.1
117114	catalase	+	1.11.1.6
117114	lysine decarboxylase	-	4.1.1.18
117114	ornithine decarboxylase	-	4.1.1.17
117114	oxidase	-
117114	urease	-	3.5.1.5

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	cellulose degradation	100	5 of 5
66794	coenzyme A metabolism	100	4 of 4
66794	aspartate and asparagine metabolism	100	9 of 9
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	acetate fermentation	100	4 of 4
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	teichoic acid biosynthesis	100	1 of 1
66794	folate polyglutamylation	100	1 of 1
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	ppGpp biosynthesis	100	4 of 4
66794	palmitate biosynthesis	95.45	21 of 22
66794	pentose phosphate pathway	90.91	10 of 11
66794	chorismate metabolism	88.89	8 of 9
66794	valine metabolism	88.89	8 of 9
66794	vitamin B1 metabolism	84.62	11 of 13
66794	peptidoglycan biosynthesis	80	12 of 15
66794	Entner Doudoroff pathway	80	8 of 10
66794	threonine metabolism	80	8 of 10
66794	vitamin K metabolism	80	4 of 5
66794	photosynthesis	78.57	11 of 14
66794	sulfopterin metabolism	75	3 of 4
66794	degradation of sugar alcohols	75	12 of 16
66794	C4 and CAM-carbon fixation	75	6 of 8
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	glycolysis	70.59	12 of 17
66794	starch degradation	70	7 of 10
66794	phenylalanine metabolism	69.23	9 of 13
66794	purine metabolism	68.09	64 of 94
66794	pyrimidine metabolism	66.67	30 of 45
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	acetoin degradation	66.67	2 of 3
66794	cyanate degradation	66.67	2 of 3
66794	serine metabolism	66.67	6 of 9
66794	formaldehyde oxidation	66.67	2 of 3
66794	NAD metabolism	66.67	12 of 18
66794	glycolate and glyoxylate degradation	66.67	4 of 6
66794	oxidative phosphorylation	64.84	59 of 91
66794	isoleucine metabolism	62.5	5 of 8
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
66794	ketogluconate metabolism	62.5	5 of 8
66794	leucine metabolism	61.54	8 of 13
66794	3-chlorocatechol degradation	60	3 of 5
66794	factor 420 biosynthesis	60	3 of 5
66794	myo-inositol biosynthesis	60	6 of 10
66794	methylglyoxal degradation	60	3 of 5
66794	metabolism of amino sugars and derivatives	60	3 of 5
66794	glycogen metabolism	60	3 of 5
66794	heme metabolism	57.14	8 of 14
66794	reductive acetyl coenzyme A pathway	57.14	4 of 7
66794	propanol degradation	57.14	4 of 7
66794	d-mannose degradation	55.56	5 of 9
66794	non-pathway related	55.26	21 of 38
66794	d-xylose degradation	54.55	6 of 11
66794	glutamate and glutamine metabolism	53.57	15 of 28
66794	histidine metabolism	51.72	15 of 29
66794	alanine metabolism	51.72	15 of 29
66794	glycogen biosynthesis	50	2 of 4
66794	selenocysteine biosynthesis	50	3 of 6
66794	ethanol fermentation	50	1 of 2
66794	degradation of hexoses	50	9 of 18
66794	CMP-KDO biosynthesis	50	2 of 4
66794	ribulose monophosphate pathway	50	1 of 2
66794	suberin monomers biosynthesis	50	1 of 2
66794	butanoate fermentation	50	2 of 4
66794	mannosylglycerate biosynthesis	50	1 of 2
66794	gluconeogenesis	50	4 of 8
66794	cis-vaccenate biosynthesis	50	1 of 2
66794	tryptophan metabolism	47.37	18 of 38
66794	degradation of pentoses	46.43	13 of 28
66794	methionine metabolism	46.15	12 of 26
66794	arginine metabolism	45.83	11 of 24
66794	proline metabolism	45.45	5 of 11
66794	vitamin B6 metabolism	45.45	5 of 11
66794	lipid metabolism	45.16	14 of 31
66794	CO2 fixation in Crenarchaeota	44.44	4 of 9
66794	tetrahydrofolate metabolism	42.86	6 of 14
66794	citric acid cycle	42.86	6 of 14
66794	ubiquinone biosynthesis	42.86	3 of 7
66794	mevalonate metabolism	42.86	3 of 7
66794	glutathione metabolism	42.86	6 of 14
66794	glycine metabolism	40	4 of 10
66794	lipoate biosynthesis	40	2 of 5
66794	cysteine metabolism	38.89	7 of 18
66794	isoprenoid biosynthesis	38.46	10 of 26
66794	metabolism of disaccharids	36.36	4 of 11
66794	lysine metabolism	35.71	15 of 42
66794	IAA biosynthesis	33.33	1 of 3
66794	degradation of aromatic, nitrogen containing compounds	33.33	4 of 12
66794	flavin biosynthesis	33.33	5 of 15
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	octane oxidation	33.33	1 of 3
66794	enterobactin biosynthesis	33.33	1 of 3
66794	urea cycle	30.77	4 of 13
66794	phenylpropanoid biosynthesis	30.77	4 of 13
66794	propionate fermentation	30	3 of 10
66794	coenzyme M biosynthesis	30	3 of 10
66794	phenol degradation	30	6 of 20
66794	tyrosine metabolism	28.57	4 of 14
66794	ascorbate metabolism	27.27	6 of 22
66794	cyclohexanol degradation	25	1 of 4
66794	lactate fermentation	25	1 of 4
66794	biotin biosynthesis	25	1 of 4
66794	dTDPLrhamnose biosynthesis	25	2 of 8
66794	degradation of sugar acids	24	6 of 25
66794	sulfate reduction	23.08	3 of 13
66794	arachidonic acid metabolism	22.22	4 of 18
66794	nitrate assimilation	22.22	2 of 9
66794	lipid A biosynthesis	22.22	2 of 9

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
117114	not determinedn.d.	+	-	-	-	+	-	-	-	-	-	+	+	+	-	+	-	-	+	-	-	-	+	+	+	+	+	+	+	-	-	-	+	-	-	-	-	-	-	+	+	-	-	-	-	-	-	+/-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
1727	soil
117114	Environment, Soil	France	FRA	Europe

Taxonmaps

69479

Global distribution of 16S sequence AY543038 (>99% sequence identity) for Brochothrix from Microbeatlas

Aquatic Samples	5597
Soil Samples	3704
Animal Samples	29797
Plant Samples	1458
Total Samples	40556

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
1727	1	Risk group (German classification) According to TRBA 466
117114	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	NCBI tax ID	Score
124043	ASM4605894v1 assembly for Brochothrix campestris DSM 4712	complete	GCA_046058945	2757	93.58

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	B.campestris gene for 16S ribosomal RNA	X56156	1480	2757
20218	Brochothrix campestris isolate DSMZ 4712 16S ribosomal RNA gene, partial sequence	AY543038	1420	2757
20218	Brochothrix campestris gene for 16S rRNA, partial sequence, strain: NBRC 15547	AB680897	1473	2757

GC content

1727

GC-content (mol%)38.0

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM4605894v1 assembly for Brochothrix campestris DSM 4712
NCBI: GCA_046058945

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	93.73	no
125439	gram_stain	BacteriaNetⓘ	positive	58.38	no
125439	motility	BacteriaNetⓘ	yes	43.48	no
125439	spore_formation	BacteriaNetⓘ	no	77.17	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Genomic Relationship Between Brochothrix campestris and Its Phages: A Cross-Replicon and Interspecies Perspective.	Attere SA, Piche LC, Vincent AT.	Genes (Basel)	10.3390/genes16101218	2025
Biotechnology	Insight into the Genome of Brochothrix thermosphacta, a Problematic Meat Spoilage Bacterium.	Stanborough T, Fegan N, Powell SM, Tamplin M, Chandry PS.	Appl Environ Microbiol	10.1128/aem.02786-16	2017
Enzymology	Development of a rapid real-time PCR method as a tool to quantify viable Photobacterium phosphoreum bacteria in salmon (Salmo salar) steaks.	Mace S, Mamlouk K, Chipchakova S, Prevost H, Joffraud JJ, Dalgaard P, Pilet MF, Dousset X.	Appl Environ Microbiol	10.1128/aem.03677-12	2013
Pathogenicity	Gene cluster responsible for secretion of and immunity to multiple bacteriocins, the NKR-5-3 enterocins.	Ishibashi N, Himeno K, Masuda Y, Perez RH, Iwatani S, Zendo T, Wilaipun P, Leelawatcharamas V, Nakayama J, Sonomoto K.	Appl Environ Microbiol	10.1128/aem.02312-14	2014
	Detection of bacteriocins by matrix-assisted laser Desorption/Ionization time-of-flight mass spectrometry	Rose NL, Sporns P, McMullen LM.	Appl Environ Microbiol	10.1128/aem.65.5.2238-2242.1999	1999
	Molecular and genetic characterization of a novel bacteriocin locus in Enterococcus avium isolates from infants.	Birri DJ, Brede DA, Forberg T, Holo H, Nes IF.	Appl Environ Microbiol	10.1128/aem.01597-09	2010
Metabolism	Isolation and characterization of carnocyclin a, a novel circular bacteriocin produced by Carnobacterium maltaromaticum UAL307.	Martin-Visscher LA, van Belkum MJ, Garneau-Tsodikova S, Whittal RM, Zheng J, McMullen LM, Vederas JC.	Appl Environ Microbiol	10.1128/aem.00817-08	2008
Metabolism	The inhibitory spectrum of thermophilin 9 from Streptococcus thermophilus LMD-9 depends on the production of multiple peptides and the activity of BlpG(St), a thiol-disulfide oxidase.	Fontaine L, Hols P.	Appl Environ Microbiol	10.1128/aem.02030-07	2008
Enzymology	Purification and characterization of multiple bacteriocins and an inducing peptide produced by Enterococcus faecium NKR-5-3 from Thai fermented fish.	Ishibashi N, Himeno K, Fujita K, Masuda Y, Perez RH, Zendo T, Wilaipun P, Leelawatcharamas V, Nakayama J, Sonomoto K	Biosci Biotechnol Biochem	10.1271/bbb.110972	2012
Biotechnology	Control of meatborne Listeria monocytogenes and Brochothrix thermosphacta by a bacteriocinogenic Brochothrix campestris ATCC 43754.	Greer GG, Dilts BD	Food Microbiol	10.1016/j.fm.2006.02.009	2006
Metabolism	Purification and characterization of brochocin A and brochocin B(10-43), a functional fragment generated by heterologous expression in Carnobacterium piscicola.	Garneau S, Ference CA, van Belkum MJ, Stiles ME, Vederas JC	Appl Environ Microbiol	10.1128/AEM.69.3.1352-1358.2003	2003
Pathogenicity	The outer membrane of gram-negative bacteria inhibits antibacterial activity of brochocin-C.	Gao Y, van Belkum MJ, Stiles ME	Appl Environ Microbiol	10.1128/AEM.65.10.4329-4333.1999	1999
Pathogenicity	Genetic characterization and heterologous expression of brochocin-C, an antibotulinal, two-peptide bacteriocin produced by Brochothrix campestris ATCC 43754.	McCormick JK, Poon A, Sailer M, Gao Y, Roy KL, McMullen LM, Vederas JC, Stiles ME, Van Belkum MJ	Appl Environ Microbiol	10.1128/AEM.64.12.4757-4766.1998	1998
Biotechnology	Brochocin-C, a new bacteriocin produced by Brochothrix campestris.	Siragusa GR, Cutter CN	Appl Environ Microbiol	10.1128/aem.59.7.2326-2328.1993	1993

References

#1727	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 4712
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#41201	; Curators of the CIP;
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#68371	Automatically annotated from API 50CH acid .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#117114	Collection of Institut Pasteur ; Curators of the CIP; CIP 102920
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

About us

Brochothrix campestris (DSM 4712, ATCC 43754, CIP 102920)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Halophily

Murein

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

Pathway annotation

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_046058945

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media