Name: Fructobacillus pseudoficulneus LC-51
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6863

Type strain:

Species: Fructobacillus pseudoficulneus

Strain Designation: LC-51, LC51

Culture col. no.: DSM 15468, CECT 5759, JCM 17832, BCRC 81234, CIP 109309, LMG 23899, NCIMB 14364, NRIC 0698

Strain history: CIP <- 2006, CECT <- 2002, L. Chambel, Lisboa Univ., Lisboa, Portugal: strain LC51

NCBI tax ID(s): 1121907 (strain), 220714 (species)

SI-ID 194264

CECT 5759, DSM 15468, LMG 23899, CIP 109309, JCM 17832

Section

Fructobacillus pseudoficulneus LC-51 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from fruit, ripe fig.

Gram-positive
ovoid-shaped
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Fructobacillus*
	Species	**Fructobacillus pseudoficulneus**
	Full Scientific Name (LPSN)	Fructobacillus pseudoficulneus (Chambel et al. 2006) Endo and Okada 2008

Synonym

Leuconostoc pseudoficulneum

Information on morphological and physiological properties Morphology

[Ref.: #31686]	Gram stain	positive
[Ref.: #121986]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #31686]	Cell length	2.2-3 µm

[Ref.: #31686]	Cell width	0.7-1.1 µm

[Ref.: #31686]	Cell shape	ovoid-shaped
[Ref.: #121986]	Cell shape	rod-shaped

[Ref.: #31686]	Motility	no
[Ref.: #121986]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %96.284

[Ref.: #31686]

Pigment production

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5941]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #5941]

Culture medium growth

[Ref.: #5941]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5941]

Culture medium composition

expand / minimize

[Ref.: #37507]

Culture medium

MEDIUM 40- for Lactobacillus and Leuconostoc

[Ref.: #37507]

Culture medium growth

[Ref.: #37507]

Culture medium composition

expand / minimize

[Ref.: #121986]

Culture medium

CIP Medium 40

[Ref.: #121986]

Culture medium growth

[Ref.: #121986]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #5941]		growth	30 °C
[Ref.: #31686]		growth	30-37 °C
[Ref.: #31686]		optimum	30 °C
[Ref.: #37507]		growth	30 °C
[Ref.: #67770]		growth	30 °C
[Ref.: #121986]		growth	15-37 °C
[Ref.: #121986]	no	growth	45 °C

[Ref.: #5941]	Temperature range	mesophilic
[Ref.: #31686]	Temperature range	mesophilic
[Ref.: #37507]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #121986]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #31686]			optimum	6.5-7
[Ref.: #31686]			growth	4.8-8.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #5941]	Oxygen tolerance	microaerophile
[Ref.: #31686]	Oxygen tolerance	facultative anaerobe
[Ref.: #121986]	Oxygen tolerance	obligate aerobe

[Ref.: #31686]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.999

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31686]		NaCl		growth	03-05	%
[Ref.: #31686]		NaCl		optimum	03-05	%

[Ref.: #31686]

Observation

aggregates in chains

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31686]	mannitol ChEBI	+	carbon source
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #121986]	nitrate ChEBI	-	reduction
[Ref.: #121986]	nitrate ChEBI	+	respiration
[Ref.: #121986]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #121986]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #121986]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #31686]	acid phosphatase	+	3.1.3.2
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #121986]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #31686]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #121986]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #121986]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #121986]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #121986]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #121986]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #121986]	11519	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	+/-	+	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+/-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121986]	11780	-	+	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5941]	Sample type/isolated from	fruit, ripe fig
[Ref.: #5941]	Country	Portugal
[Ref.: #5941]	Country ISO 3 Code	PRT
[Ref.: #5941]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Ripe fig
[Ref.: #67770]	Geographic location (country and/or sea, region)	Alentejo region
[Ref.: #67770]	Country	Portugal
[Ref.: #67770]	Country ISO 3 Code	PRT
[Ref.: #67770]	Continent	Europe

[Ref.: #121986]	Sample type/isolated from	Ripe fig
[Ref.: #121986]	Geographic location (country and/or sea, region)	Alentejo
[Ref.: #121986]	Country	Portugal
[Ref.: #121986]	Country ISO 3 Code	PRT
[Ref.: #121986]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host Body-Site

#Plant

#Fruit (Seed)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC519998 (>99% sequence identity) for Fructobacillus from Microbeatlas

Aquatic Samples	157
Soil Samples	61
Animal Samples	1617
Plant Samples	148
Total Samples	1983

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5941]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #121986]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Fructobacillus pseudoficulneus JCM 17832 gene for 16S rRNA, partial sequence	LC519998	1481	ENA	220714 tax ID
[Ref.: #5941]		AY16997		ENA
[Ref.: #31686]	Fructobacillus pseudoficulneus strain LC51 16S ribosomal RNA gene, partial sequence	AY169967	1494	GenBank	220714 tax ID
[Ref.: #20218]	Fructobacillus pseudoficulneus DNA, 16S-23S rRNA intergenic spacer region, strain: DSM 15468	AB290447	363	ENA	220714 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Fructobacillus pseudoficulneus DSM 15468	GCA_001047115	scaffold	GenBank	220714 tax ID
[Ref.: #67770]	Fructobacillus pseudoficulneus DSM 15468	GCA_900108455	scaffold	GenBank	220714 tax ID
[Ref.: #66792]	Fructobacillus pseudoficulneus strain DSM 15468	220714.4	wgs	PATRIC	220714 tax ID	*
[Ref.: #66792]	Fructobacillus pseudoficulneus strain DSM 15468	220714.3	wgs	PATRIC	220714 tax ID	*
[Ref.: #66792]	Fructobacillus pseudoficulneus DSM 15468	2814123065	draft	JGI IMG/M	220714 tax ID	*
[Ref.: #66792]	Fructobacillus pseudoficulneus DSM 15468	2600254955	draft	JGI IMG/M	220714 tax ID	*

[Ref.: #5941]	GC-content	44.5 mol%
[Ref.: #67770]	GC-content	44.5 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Fructobacillus pseudoficulneus DSM 15468 NCBI: GCA_900108455.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Fructobacillus pseudoficulneus strain DSM 15468 PATRIC: 220714.4
[Ref.: #69480]	motile	no	96.904	yes
[Ref.: #69480]	gram-positive	yes	94.064	no
[Ref.: #69480]	anaerobic	no	84.321	no
[Ref.: #69480]	aerobic	no	97.315	yes
[Ref.: #69480]	halophile	yes	89.952	no
[Ref.: #69480]	spore-forming	no	96.719	no
[Ref.: #69480]	glucose-util	yes	84.555	no
[Ref.: #69480]	flagellated	no	98.656	no
[Ref.: #69480]	thermophile	no	98.707	no
[Ref.: #69480]	glucose-ferment	yes	86.621	no

Availability in culture collections External links

[Ref.: #5941]

Culture collection no.

DSM 15468, CECT 5759, JCM 17832, BCRC 81234, CIP 109309, LMG 23899, NCIMB 14364, NRIC 0698

[Ref.: #76328]

SI-ID 194264

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Reclassification of the genus Leuconostoc and proposals of Fructobacillus fructosus gen. nov., comb. nov., Fructobacillus durionis comb. nov., Fructobacillus ficulneus comb. nov. and Fructobacillus pseudoficulneus comb. nov.	Endo A, Okada S	Int J Syst Evol Microbiol	10.1099/ijs.0.65609-0	2008	*
Phylogeny	Leuconostoc pseudoficulneum sp. nov., isolated from a ripe fig.	Chambel L, Chelo IM, Ze-Ze L, Pedro LG, Santos MA, Tenreiro R	Int J Syst Evol Microbiol	10.1099/ijs.0.64054-0	2006	*

References

#5941

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15468

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#31686

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27963 (see below)

#37507 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#76328

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID194264.1 )

#121986 Collection of Institut Pasteur ; Curators of the CIP; CIP 109309

#27963

IJSEM 1375 2006 ( DOI 10.1099/ijs.0.64054-0 , PubMed 16738117 )

* These data were automatically processed and therefore are not curated

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Fructobacillus pseudoficulneus DSM 15468

Fructobacillus pseudoficulneus strain DSM 15468

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Change proposal