Name: Weissella hellenica LV346
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6852

Type strain:

Species: Weissella hellenica

Strain Designation: LV346

Culture col. no.: DSM 7378, ATCC 51523, NCFB 2973, CCUG 33494, LMG 15125, JCM 10103, CECT 7033, CIP 109879, IFO 15553, KCTC 3668, NBRC 15553, NCIMB 702973

Strain history: CIP <- 2008, JCM <- M.D. Collins, Reading lab., UK <- NCFB 2973 <- J. Samelis LV346: strain 10103

NCBI tax ID(s): 46256 (species)

SI-ID 8831

LMG 15125, ATCC 51523, CCUG 33494, DSM 7378, IFO 15553, NCFB 2973, JCM 10103, NCIMB 702973, IFO1553, KCTC 3668, NBRC 15553, CECT 7033, CIP 109879

Other strains from Weissella hellenica

W. hellenica SS 80W, CIP 106375, NCDO 885
W. hellenica SS98, CIP 106376, NCDO 886
W. hellenica

Section

Weissella hellenica LV346 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from sausage; naturally-fermented.

Gram-positive
oval-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Weissella*
	Species	**Weissella hellenica**
	Full Scientific Name (LPSN)	Weissella hellenica Collins et al. 1994

Information on morphological and physiological properties Morphology

[Ref.: #119862]	Gram stain	positive

[Ref.: #119862]	Cell shape	oval-shaped

[Ref.: #119862]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #3134]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #3134]

Culture medium growth

[Ref.: #3134]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3134]

Culture medium composition

expand / minimize

[Ref.: #40564]

Culture medium

MEDIUM 40- for Lactobacillus and Leuconostoc

[Ref.: #40564]

Culture medium growth

[Ref.: #40564]

Culture medium composition

expand / minimize

[Ref.: #119862]

Culture medium

CIP Medium 40

[Ref.: #119862]

Culture medium growth

[Ref.: #119862]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #3134]		growth	30 °C
[Ref.: #40564]		growth	25 °C
[Ref.: #51552]		growth	20-25 °C
[Ref.: #67770]		growth	30 °C
[Ref.: #119862]		growth	15-30 °C
[Ref.: #119862]	no	growth	37 °C
[Ref.: #119862]	no	growth	45 °C

[Ref.: #3134]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #119862]	Temperature range	mesophilic
[Ref.: #119862]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #51552]	Oxygen tolerance	aerobe
[Ref.: #119862]	Oxygen tolerance	facultative anaerobe

[Ref.: #3134]	Murein short key	A11.09
[Ref.: #3134]	Murein types	A3alpha L-Lys-L-Ala-L-Ala(L-Ser)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #119862]	nitrate ChEBI	-	reduction
[Ref.: #119862]	nitrate ChEBI	+	respiration
[Ref.: #119862]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #119862]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #119862]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #119862]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #119862]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #119862]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #119862]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #51552]

Fatty acid	Percentage	ECL
C18:1 ω7c /12t/9t	43.9	17.824
C16:0	25.9	16
C18:2 ω6,9c/C18:0 ANTE	8.3	17.724
C18:1 ω9c	7.1	17.769
C18:0	4.3	18
C16:1 ω7c/C15:0 ISO 2OH	4.1	15.85
C16:1 ω7c	4	15.819
C14:0	2.5	14

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #3134]	14257	-	-	-	-	+	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	+/-	-	-	-	-	-	+	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	+	-	+	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #119862]	12186	-	+	-	-	-	-	-	-	-	-	+	+	-	+	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #3134]	Sample type/isolated from	sausage; naturally-fermented
[Ref.: #3134]	Country	Greece
[Ref.: #3134]	Country ISO 3 Code	GRC
[Ref.: #3134]	Continent	Europe

[Ref.: #51552]	Sample type/isolated from	Dry naturally fermented Greek sausage

[Ref.: #67770]	Sample type/isolated from	Fermented sausage

[Ref.: #119862]	Sample type/isolated from	Fermented sausage
[Ref.: #119862]	Geographic location (country and/or sea, region)	Reading
[Ref.: #119862]	Country	United Kingdom
[Ref.: #119862]	Country ISO 3 Code	GBR
[Ref.: #119862]	Continent	Europe
[Ref.: #119862]	Isolation date	1993
* marker position based on {}

Isolation sources categories

#Engineered

#Food production

#Meat

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence S67831 (>99% sequence identity) for Weissella hellenica from Microbeatlas

Aquatic Samples	1465
Soil Samples	1440
Animal Samples	13049
Plant Samples	1330
Total Samples	17284

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #3134]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #119862]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Weissella hellenica gene for 16S ribosomal RNA, partial sequence, strain: JCM 10103	LC096226	1512	ENA	46256 tax ID
[Ref.: #20218]	Weissella hellenica gene for 16S rRNA, partial sequence, strain: NBRC 15553	AB680902	1496	ENA	46256 tax ID	*
[Ref.: #20218]	Weissella hellenica strain NCFB 2973 16S ribosomal RNA gene, partial sequence	S67831	1527	ENA	46256 tax ID	*
[Ref.: #20218]	W.hellenica 16S rRNA gene	X95981	1334	ENA	46256 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Weissella hellenica NBRC 15553	GCA_005405545	scaffold	GenBank	46256 tax ID
[Ref.: #67770]	Weissella hellenica NBRC 15553	GCA_006540065	contig	GenBank	46256 tax ID
[Ref.: #67770]	Weissella hellenica CCUG 33494	GCA_012396435	contig	GenBank	46256 tax ID
[Ref.: #66792]	Weissella hellenica strain CCUG 33494	46256.17	wgs	PATRIC	46256 tax ID	*
[Ref.: #66792]	Weissella hellenica strain NBRC 15553	46256.14	wgs	PATRIC	46256 tax ID	*
[Ref.: #66792]	Weissella hellenica strain NBRC 15553	46256.12	wgs	PATRIC	46256 tax ID	*

[Ref.: #3134]

GC-content

39.4 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Weissella hellenica strain CCUG 33494 PATRIC: 46256.17
[Ref.: #69480]	gram-positive	yes	97.376	no
[Ref.: #69480]	anaerobic	no	94.499	no
[Ref.: #69480]	halophile	yes	96.813	no
[Ref.: #69480]	spore-forming	no	96.787	no
[Ref.: #69480]	glucose-util	yes	90.495	no
[Ref.: #69480]	aerobic	no	90.634	yes
[Ref.: #69480]	flagellated	no	98.556	no
[Ref.: #69480]	motile	no	96.808	no
[Ref.: #69480]	thermophile	no	99.827	yes
[Ref.: #69480]	glucose-ferment	yes	90.298	no

Availability in culture collections External links

[Ref.: #3134]

Culture collection no.

DSM 7378, ATCC 51523, NCFB 2973, CCUG 33494, LMG 15125, JCM 10103, CECT 7033, CIP 109879, IFO 15553, KCTC 3668, NBRC 15553, NCIMB 702973

[Ref.: #76317]

SI-ID 8831

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Novel lactic acid bacteria isolated from the bumble bee gut: Convivina intestini gen. nov., sp. nov., Lactobacillus bombicola sp. nov., and Weissella bombi sp. nov.	Praet J, Meeus I, Cnockaert M, Houf K, Smagghe G, Vandamme P	Antonie Van Leeuwenhoek	10.1007/s10482-015-0429-z	2015	*
	Specific probiotic characterization of Weissella hellenica DS-12 isolated from flounder intestine.	Cai Y, Benno Y, Nakase T, Oh TK	J Gen Appl Microbiol	10.2323/jgam.44.311	1998	*

References

#3134

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 7378

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#40564 ; Curators of the CIP;
#51552 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 33494

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#76317

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID8831.1 )

#119862 Collection of Institut Pasteur ; Curators of the CIP; CIP 109879
* These data were automatically processed and therefore are not curated

Change proposal

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Weissella hellenica strain CCUG 33494

Change proposal

Change proposal