Name: Leuconostoc inhae IH003
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 6837

Type strain:

Species: Leuconostoc inhae

Strain Designation: IH003

Culture col. no.: DSM 15101, KCTC 3774, JCM 17833, CECT 7026, CIP 108081, LMG 22919, NRIC 0562

Strain history: CIP <- 2003, DSMZ <- H. Han <- B. Kim: strain IH003

NCBI tax ID(s): 927694 (strain), 178001 (species)

SI-ID 99904

DSM 15101, KCTC 3774, CECT 7026, LMG 22919, CIP 108081, JCM 17833

Special Collections

Section

Leuconostoc inhae IH003 is an anaerobe, sphere-shaped bacterium that was isolated from kimchi .

sphere-shaped
anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Leuconostoc*
	Species	**Leuconostoc inhae**
	Full Scientific Name (LPSN)	Leuconostoc inhae Kim et al. 2003

Information on morphological and physiological properties Morphology

[Ref.: #67771]	Gram stain	positive
[Ref.: #120116]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %95.622

[Ref.: #120116]	Cell shape	coccus-shaped
[Ref.: #67771]	Cell shape	sphere-shaped

[Ref.: #120116]	Motility	no
[Ref.: #67771]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %93.5

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5712]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #5712]

Culture medium growth

[Ref.: #5712]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5712]

Culture medium composition

expand / minimize

[Ref.: #39978]

Culture medium

MEDIUM 40- for Lactobacillus and Leuconostoc

[Ref.: #39978]

Culture medium growth

[Ref.: #39978]

Culture medium composition

expand / minimize

[Ref.: #120116]

Culture medium

CIP Medium 40

[Ref.: #120116]

Culture medium growth

[Ref.: #120116]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #5712]		growth	28 °C
[Ref.: #39978]		growth	25 °C
[Ref.: #67770]		growth	30 °C
[Ref.: #67771]		growth	25 °C
[Ref.: #120116]		growth	10-25 °C
[Ref.: #120116]	no	growth	37 °C
[Ref.: #120116]	no	growth	45 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #67771]	Oxygen tolerance	anaerobe
[Ref.: #120116]	Oxygen tolerance	facultative anaerobe

[Ref.: #67771]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #120116]	nitrate ChEBI	-	reduction
[Ref.: #120116]	nitrate ChEBI	+	respiration
[Ref.: #120116]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #120116]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	yes	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #120116]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #120116]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #120116]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #120116]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #120116]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #120116]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #5712]	14320	-	-	-	-	+	+	-	-	-	-	-	+	+	+	-	-	-	-	+	-	-	+	+	-	-	+	-	+	+	-	-	+	+	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	+/-	-
[Ref.: #120116]	11190	not determinedn.d.	-	-	-	+	+	-	-	-	-	-	+	+	+	-	-	-	-	+/-	-	-	+	+	-	-	+	-	+	+	-	-	+	+	-	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	+/-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #120116]	10869	-	-	+	-	-	+	-	-	-	-	-	-	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5712]	Sample type/isolated from	kimchi (fermented vegetable food in Korea)
[Ref.: #5712]	Country	Republic of Korea
[Ref.: #5712]	Country ISO 3 Code	KOR
[Ref.: #5712]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Kimchi
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From Kimchi(fermented vegetable food in Korea)
[Ref.: #67771]	Country	Republic of Korea
[Ref.: #67771]	Country ISO 3 Code	KOR
[Ref.: #67771]	Continent	Asia

[Ref.: #120116]	Sample type/isolated from	Food, Kimchi, fermented vegetale food in Korea
[Ref.: #120116]	Country	Republic of Korea
[Ref.: #120116]	Country ISO 3 Code	KOR
[Ref.: #120116]	Continent	Asia
[Ref.: #120116]	Isolation date	1999
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Vegetable (incl. Grains)
#Engineered	#Food production	#Fermented

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC519999 (>99% sequence identity) for Leuconostoc from Microbeatlas

Aquatic Samples	66
Soil Samples	148
Animal Samples	575
Plant Samples	42
Total Samples	831

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5712]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120116]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Leuconostoc inhae JCM 17833 gene for 16S rRNA, partial sequence	LC519999	1486	GenBank ENA	178001 tax ID
[Ref.: #5712]	Leuconostoc inhae 16S ribosomal RNA gene, partial sequence	AF439560	1474	GenBank ENA	927694 tax ID
[Ref.: #124043]	Leuconostoc inhae DNA, 16S-23S rRNA intergenic spacer region, strain: NRIC 0562.	AB290440	380	GenBank ENA	178001 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Leuconostoc inhae KCTC 3774	GCA_000166735	contig	GenBank ENA	927694 tax ID
[Ref.: #66792]	Leuconostoc inhae DSM 15101	GCA_019656015	contig	GenBank ENA	178001 tax ID	*
[Ref.: #66792]	Leuconostoc inhae KCTC 3774	927694.4	wgs	PATRIC	927694 tax ID	*
[Ref.: #66792]	Leuconostoc inhae strain DSM 15101	178001.5	wgs	PATRIC	178001 tax ID	*
[Ref.: #66792]	Leuconostoc inhae Kim IH003, KCTC 3774	651324069	draft	JGI IMG/M	927694 tax ID	*

[Ref.: #5712]	GC-content	39.9±0.5 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67770]	GC-content	39.9 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67771]	GC-content	39.9 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Leuconostoc inhae KCTC 3774 PATRIC: 927694.4
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Leuconostoc inhae KCTC 3774 PATRIC: 927694.4
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	95.622	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	87.92	no
[Ref.: #69480]	aerobic	aerobicⓘ	no	94.732	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	86.199	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	96.6	no
[Ref.: #69480]	flagellated	motile2+ⓘ	no	93.5	yes

Availability in culture collections External links

[Ref.: #5712]

Culture collection no.

DSM 15101, KCTC 3774, JCM 17833, CECT 7026, CIP 108081, LMG 22919, NRIC 0562

[Ref.: #76302]

SI-ID 99904

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Leuconostoc miyukkimchii sp. nov., isolated from brown algae (Undaria pinnatifida) kimchi.	Lee SH, Park MS, Jung JY, Jeon CO	Int J Syst Evol Microbiol	10.1099/ijs.0.032367-0	2011	*
Genetics	Genome sequence of Leuconostoc inhae KCTC 3774, isolated from Kimchi.	Kim DS, Choi SH, Kim DW, Kim RN, Nam SH, Kang A, Kim A, Park HS	J Bacteriol	10.1128/JB.01458-10	2010	*
Phylogeny	Leuconostoc inhae sp. nov., a lactic acid bacterium isolated from kimchi.	Kim B, Lee J, Jang J, Kim J, Han H	Int J Syst Evol Microbiol	10.1099/ijs.0.02463-0	2003	*

References

#5712

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15101

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#39978 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#76302

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID99904.1 )

#120116 Collection of Institut Pasteur ; Curators of the CIP; CIP 108081

#124043

Dr. Isabel Schober, Dr. Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .

* These data were automatically processed and therefore are not curated

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Leuconostoc inhae KCTC 3774

Leuconostoc inhae KCTC 3774

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