Leuconostoc oeni (DSM 20252, ATCC 23279, NCDO 1674)

Name: Leuconostoc oeni (DSM 20252, ATCC 23279, NCDO 1674)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6810

Type strain

Strain Designation Baudry 1

Culture col. no. DSM 20252 ATCC 23279 NCDO 1674 Baudry 1 JCM 6125 15 more

NCBI tax ID(s) 1122618 1247

Special Collections DSMZ CCUG JCM KCTC CIP JMRC

History DSMZ <- NCDO <- E. Peynaud, Baudry 1 JCM <-- ATCC 23279 <-- NCDO 1674 <-- E. Peynaud (Baudry 1). CIP <- 1999, F. Gasser, Inst. Pasteur, Paris, France <- ATCC <- NCDO <- E. Peynaud

Links

Leuconostoc oeni Baudry 1 is a Gram-positive, oval-shaped bacterium that was isolated from wine.

Gram-positive oval-shaped genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 3504

LMG 9851,ATCC 23279,CCUG 30067,CCUG 30199,CCUG 32250,NCFB 1674,NCIMB 11648,NRRL B-3472,NCDO 1674,CIP 106144,JCM 6125,CECT 217,KCTC 3072,CECT 4100,CCRC 14062,KCTC 3612,NBRC 100497,BCRC 14062,NCIM 2219,VTT E-97832,DSM 20252

@ref 20215

Last LPSN update 2026-05-29 08:26:30

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Lactobacillaceae

Genus Leuconostoc

Species Leuconostoc oeni

Full scientific name Leuconostoc oeni corrig. Garvie 1967 (Approved Lists 1980)

Synonyms (2)

Leuconostoc oenos
Oenococcus oeni

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
121401	positive	oval-shaped
125438			91.5
125439	negative		99.109

Multimedia

@ref:	66793
multimedia content:	EM_DSM_20252_1.jpg
multimedia.multimedia content:	EM_DSM_20252_1.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_20252_2.jpg
multimedia.multimedia content:	EM_DSM_20252_2.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_20252_3.jpg
multimedia.multimedia content:	EM_DSM_20252_3.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_20252_4.jpg
multimedia.multimedia content:	EM_DSM_20252_4.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_20252_5.jpg
multimedia.multimedia content:	EM_DSM_20252_5.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
8662	LEUCONOSTOC OENOS MEDIUM (DSMZ Medium 59)	Medium recipe at MediaDive	Name: LEUCONOSTOC OENOS MEDIUM (DSMZ Medium 59) Composition: Casein peptone 9.99001 g/l Glucose 9.99001 g/l Fructose 4.99501 g/l Yeast extract 4.99501 g/l (NH4) citrate 3.4965 g/l L-Cysteine HCl x H2O 0.499501 g/l MgSO4 x 7 H2O 0.1998 g/l MnSO4 x H2O 0.04995 g/l Tomato juice Tween 80 Distilled water
40293	MEDIUM 292 - for Oenococcus oeni		Distilled water make up to (1000.000 ml);ManganeseII chloride tetrahydrate(0.050 g);Magnesium sulphate heptahydrate (0.200 g);Agar (15.000 g);Glucose (10.000 g);Yeast extract (5.000 g);Peptone (10.000 g);Filtered tomato Juice (250.000 ml)
121401	CIP Medium 292	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
8662	positive	growth	30
40293	positive	growth	22
67770	positive	growth	30
121401	positive	growth	22-30
121401	negative	growth	10
121401	negative	growth	15
121401	negative	growth	37
121401	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	99.528

Spore formation

@ref	Spore formation	Confidence
125438		92.511
125439		99.871

Murein

@ref	Murein short key	Type
8662	A11.18	A3alpha L-Lys-L-Ser-L-Ala

Observation

67770

ObservationAssay of Tomato juice factor

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
121401	17632 ChEBI	nitrate	-	builds gas from
121401	17632 ChEBI	nitrate	-	reduction
121401	16301 ChEBI	nitrite	-	builds gas from
121401	16301 ChEBI	nitrite	-	reduction

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
121401	15688 ChEBI	acetoin	-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
121401	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
121401	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	-		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
121401	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
121401	ornithine decarboxylase	-	4.1.1.17
121401	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
121401	-	-	-	-	-	+	+	-	-	-	+	+	-	-	-	+	+	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Food production	#Beverage
#Engineered	#Food production	#Fermented
#Host	#Plants	#Shrub (Scrub)
#Host Body-Site	#Plant	#Fruit (Seed)

Isolation

@ref	Sample type
8662	wine
67770	Wine
121401	Food, Wine

Taxonmaps

69479

Global distribution of 16S sequence LC071842 (>99% sequence identity) for Oenococcus oeni subclade from Microbeatlas

Aquatic Samples	173
Soil Samples	380
Animal Samples	1270
Plant Samples	170
Total Samples	1993

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
8662	1	Risk group (German classification) According to TRBA 466
121401	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM37248v1 assembly for Oenococcus oeni DSM 20252 = AWRIB129	contig	GCA_000372485	1122618.5	2515154139	1122618	72.84
66792	AWRIB129_v1 assembly for Oenococcus oeni DSM 20252 = AWRIB129	contig	GCA_000309445	1122618.3	2537562032	1122618	70.2

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Oenococcus oeni gene for 16S rRNA, strain:JCM 6125	AB022924	1471	1247
20218	Oenococcus oeni gene for 16S rRNA, partial sequence, strain: JCM 6125	AB596939	1486	1247
20218	Oenococcus oeni gene for 16S rRNA, partial sequence, strain: NBRC 100497	AB681195	1489	1247
20218	Leuconostoc oenos gene for 16S ribosomal RNA, partial sequence	D31673	208	1247
20218	O.oeni 16S ribosomal RNA	X95980	1335	1247
20218	L.oenos DNA for 16S-23S intergenic spacer region	X90455	495	1247
67770	Oenococcus oeni gene for 16S ribosomal RNA, partial sequence, strain: JCM 6125	LC071842	1506	1247
67770	L.oenos 16S small subunit ribosomal RNA	M35820	1510	1247

GC content

@ref	GC-content (mol%)	Method
8662	38.9
67770	38.9	thermal denaturation, midpoint method (Tm)
67770	42	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM37248v1 assembly for Oenococcus oeni DSM 20252 = AWRIB129
NCBI: GCA_000372485

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.53	no
125439	gram_stain	BacteriaNetⓘ	negative	99.11	no
125439	motility	BacteriaNetⓘ	yes	58.74	no
125439	spore_formation	BacteriaNetⓘ	no	99.87	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Oenococcus oeni DSM 20252 = AWRIB129
PATRIC: 1122618.5

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	89.08	no
125438	anaerobic	anaerobicⓘ	no	74.85	no
125438	spore-forming	spore-formingⓘ	no	92.51	no
125438	aerobic	aerobicⓘ	no	94.67	no
125438	thermophilic	thermophileⓘ	no	91.83	yes
125438	flagellated	motile2+ⓘ	no	91.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Unveiling the Potential of Lentilactobacillus hilgardii in Malolactic Fermentation: Comparative Genomics and Fermentation Dynamics.	Mantegazza G, Mangieri N, Yazdi EV, Russo P, Mora D, Gargari G.	Microb Biotechnol	10.1111/1751-7915.70259	2025
Genetics	Complete genome sequence of Oenococcus oeni strain K19-3 isolated from grape must.	Mardanov AV, Beletsky AV, Vasyagin EA, Tanashchuk TN, Shalamitskiy MY, Ravin NV.	Microbiol Resour Announc	10.1128/mra.00988-23	2024
	Statistical Analysis and Optimization of the Brilliant Red HE-3B Dye Biosorption onto a Biosorbent Based on Residual Biomass.	Suditu GD, Blaga AC, Tataru-Farmus RE, Zaharia C, Suteu D.	Materials (Basel)	10.3390/ma15207180	2022
	Statistical modeling-approach for optimization of Cu²⁺ biosorption by Azotobacter nigricans NEWG-1; characterization and application of immobilized cells for metal removal.	Ghoniem AA, El-Naggar NE, Saber WIA, El-Hersh MS, El-Khateeb AY.	Sci Rep	10.1038/s41598-020-66101-x	2020
Enzymology	Malolactic enzyme from Oenococcus oeni: heterologous expression in Escherichia coli and biochemical characterization.	Schumann C, Michlmayr H, Del Hierro AM, Kulbe KD, Jiranek V, Eder R, Nguyen TH.	Bioengineered	10.4161/bioe.22988	2013
	Heterologous expression of Oenococcus oeni malolactic enzyme in Lactobacillus plantarum for improved malolactic fermentation.	Schumann C, Michlmayr H, Eder R, Del Hierro AM, Kulbe KD, Mathiesen G, Nguyen TH.	AMB Express	10.1186/2191-0855-2-19	2012
	Comparative analysis of the Oenococcus oeni pan genome reveals genetic diversity in industrially-relevant pathways.	Borneman AR, McCarthy JM, Chambers PJ, Bartowsky EJ.	BMC Genomics	10.1186/1471-2164-13-373	2012
Genetics	GroopM: an automated tool for the recovery of population genomes from related metagenomes.	Imelfort M, Parks D, Woodcroft BJ, Dennis P, Hugenholtz P, Tyson GW.	PeerJ	10.7717/peerj.603	2014
Genetics	Exopolysaccharide (EPS) synthesis by Oenococcus oeni: from genes to phenotypes.	Dimopoulou M, Vuillemin M, Campbell-Sills H, Lucas PM, Ballestra P, Miot-Sertier C, Favier M, Coulon J, Moine V, Doco T, Roques M, Williams P, Petrel M, Gontier E, Moulis C, Remaud-Simeon M, Dols-Lafargue M.	PLoS One	10.1371/journal.pone.0098898	2014
Metabolism	Riboflavin production in Lactococcus lactis: potential for in situ production of vitamin-enriched foods.	Burgess C, O'connell-Motherway M, Sybesma W, Hugenholtz J, van Sinderen D.	Appl Environ Microbiol	10.1128/aem.70.10.5769-5777.2004	2004
Genetics	The proteolytic system of lactic acid bacteria revisited: a genomic comparison.	Liu M, Bayjanov JR, Renckens B, Nauta A, Siezen RJ.	BMC Genomics	10.1186/1471-2164-11-36	2010
	Freon 11 extraction of volatile metabolites formed by certain lactic Acid bacteria.	Tracey RP, Britz TJ.	Appl Environ Microbiol	10.1128/aem.55.6.1617-1623.1989	1989
Genetics	Oenococcus oeni genome plasticity is associated with fitness.	Bon E, Delaherche A, Bilhere E, De Daruvar A, Lonvaud-Funel A, Le Marrec C.	Appl Environ Microbiol	10.1128/aem.02194-08	2009
Phylogeny	Allelic diversity and population structure in Oenococcus oeni as determined from sequence analysis of housekeeping genes.	de Las Rivas B, Marcobal A, Munoz R.	Appl Environ Microbiol	10.1128/aem.70.12.7210-7219.2004	2004
Enzymology	Real-time quantitative PCR (QPCR) and reverse transcription-QPCR for detection and enumeration of total yeasts in wine.	Hierro N, Esteve-Zarzoso B, Gonzalez A, Mas A, Guillamon JM.	Appl Environ Microbiol	10.1128/aem.00388-06	2006
	Production of d-Branched-Chain Amino Acids by Lactic Acid Bacteria Carrying Homologs to Isoleucine 2-Epimerase of Lactobacillus buchneri.	Mutaguchi Y, Kasuga K, Kojima I.	Front Microbiol	10.3389/fmicb.2018.01540	2018
	The rapid identification of lactic acid bacteria present in Chilean winemaking processes using culture-independent analysis.	Ilabaca C, Jara C, Romero J.	Ann Microbiol	10.1007/s13213-014-0810-6	2014
Genetics	Shotgun Metagenomics of a Water Kefir Fermentation Ecosystem Reveals a Novel Oenococcus Species.	Verce M, De Vuyst L, Weckx S.	Front Microbiol	10.3389/fmicb.2019.00479	2019
Phylogeny	Bifidobacterium lactis DSM 10140: identification of the atp (atpBEFHAGDC) operon and analysis of its genetic structure, characteristics, and phylogeny.	Ventura M, Canchaya C, van Sinderen D, Fitzgerald GF, Zink R.	Appl Environ Microbiol	10.1128/aem.70.5.3110-3121.2004	2004
	Development and Validation of a New Method for Detecting Acetic Bacteria in Wine.	Parra A, Ovejas A, Gonzalez-Arenzana L, Gutierrez AR, Lopez-Alfaro I.	Foods	10.3390/foods12203734	2023
	Evaluation of Malolactic Bacteria Associated with Wines from Albariño Variety as Potential Starters: Screening for Quality and Safety.	Lopez-Seijas J, Garcia-Fraga B, da Silva AF, Zas-Garcia X, Lois LC, Gago-Martinez A, Leao-Martins JM, Sieiro C.	Foods	10.3390/foods9010099	2020
Genetics	Genome-Scale Reconstruction of the Metabolic Network in Oenococcus oeni to Assess Wine Malolactic Fermentation.	Mendoza SN, Canon PM, Contreras A, Ribbeck M, Agosin E.	Front Microbiol	10.3389/fmicb.2017.00534	2017
Metabolism	Oenococcus oeni cells immobilized on delignified cellulosic material for malolactic fermentation of wine.	Agouridis N, Kopsahelis N, Plessas S, Koutinas AA, Kanellaki M	Bioresour Technol	10.1016/j.biortech.2008.04.026	2008
Metabolism	Studies on growth and metabolism of Oenococcus oeni on sugars and sugar mixtures.	Zhang DS, Lovitt RW	J Appl Microbiol	10.1111/j.1365-2672.2005.02628.x	2005
Metabolism	Performance assessment of malolactic fermenting bacteria Oenococcus oeni and Lactobacillus brevis in continuous culture.	Zhang D, Lovitt RW	Appl Microbiol Biotechnol	10.1007/s00253-005-0021-y	2005
Phylogeny	Proposal to reclassify Leuconostoc oenos as Oenococcus oeni [corrig.] gen. nov., comb. nov..	Dicks LM, Dellaglio F, Collins MD	Int J Syst Bacteriol	10.1099/00207713-45-2-395	1995

References

#8662	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20252
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20216	Curators of the JMRC: Jena Microbial Resource Collection (JMRC):
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#40293	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66793	Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#121401	Collection of Institut Pasteur ; Curators of the CIP; CIP 106144
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Leuconostoc oeni (DSM 20252, ATCC 23279, NCDO 1674)

Name and taxonomic classification

Morphology

Cell morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Murein

Observation

Metabolite utilization

Metabolite tests

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000372485

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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