Name: Leptospira broomii 5399
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6806

Type strain:

Species: Leptospira broomii

Strain Designation: 5399

Culture col. no.: DSM 21534, ATCC BAA-1107, KIT 5399

Strain history: <- R. Galloway, CDC; strain 5399 {2008} <- P. N. Levett

NCBI tax ID(s): 301541 (species)

SI-ID 290984

ATCC BAA-1107

Section

Leptospira broomii 5399 is a mesophilic bacterium that was isolated from human patient with leptospirosis.

mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Spirochaetota*
	Class	*Spirochaetia*
	Order	*Leptospirales*
	Family	*Leptospiraceae*
	Genus	*Leptospira*
	Species	**Leptospira broomii**
	Full Scientific Name (LPSN)	Leptospira broomii Levett et al. 2006

Information on culture and growth conditions Culture and growth conditions

[Ref.: #15766]

Culture medium

LEPTOSPIRA-MEDIUM (DSMZ Medium 1113)

[Ref.: #15766]

Culture medium growth

[Ref.: #15766]

Culture medium link

Medium recipe at MediaDive

[Ref.: #15766]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #15766]			growth	30 °C

[Ref.: #15766]

Temperature range

mesophilic

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #15766]	Sample type/isolated from	human patient with leptospirosis
[Ref.: #15766]	Country	Denmark
[Ref.: #15766]	Country ISO 3 Code	DNK
[Ref.: #15766]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Infection	#Disease	-
#Infection	#Patient	-
#Host	#Human	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY796065 (>99% sequence identity) for Leptospira from Microbeatlas

Aquatic Samples	1106
Soil Samples	169
Animal Samples	1640
Plant Samples	92
Total Samples	3007

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #15766]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #15766]	Leptospira broomii strain 5399 16S ribosomal RNA gene, partial sequence	AY796065	1431	ENA	301541 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Leptospira broomii serovar Hurstbridge str. 5399	GCA_000243715	contig	GenBank	1049789 tax ID	*
[Ref.: #66792]	Leptospira broomii serovar Hurstbridge str. 5399	1049789.4	wgs	PATRIC	1049789 tax ID	*
[Ref.: #66792]	Leptospira broomii 5399	2541047153	draft	JGI IMG/M	1049789 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Leptospira broomii serovar Hurstbridge str. 5399 PATRIC: 1049789.4
[Ref.: #69480]	motile	yes	87.808	no
[Ref.: #69480]	flagellated	no	69.123	no
[Ref.: #69480]	gram-positive	no	97.717	no
[Ref.: #69480]	anaerobic	no	97.175	no
[Ref.: #69480]	aerobic	yes	81.238	no
[Ref.: #69480]	halophile	no	93.725	no
[Ref.: #69480]	spore-forming	no	91.392	no
[Ref.: #69480]	thermophile	no	95.195	no
[Ref.: #69480]	glucose-util	yes	60.036	no
[Ref.: #69480]	glucose-ferment	no	88.622	no

Availability in culture collections External links

[Ref.: #15766]

Culture collection no.

DSM 21534, ATCC BAA-1107, KIT 5399

[Ref.: #76274]

SI-ID 290984

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Leptospira broomii sp. nov., isolated from humans with leptospirosis.	Levett PN, Morey RE, Galloway RL, Steigerwalt AG	Int J Syst Evol Microbiol	10.1099/ijs.0.63783-0	2006	*

References

#15766

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21534

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#76274

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID290984.1 )

* These data were automatically processed and therefore are not curated

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Leptospira broomii serovar Hurstbridge str. 5399

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