Legionella massiliensis (DSM 24804, CSUR P146, LegA)

Name: Legionella massiliensis (DSM 24804, CSUR P146, LegA)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6788

Type strain

Strain Designation LegA

Culture col. no. DSM 24804 CSUR P146 LegA

NCBI tax ID(s) 1034943

Special Collections DSMZ Literature strains

History DSMZ <- A. Campocasso, URMITE, Univ. Méditerranée, Marseille, France; LegA

Links

Legionella massiliensis LegA is a Gram-negative, rod-shaped bacterium that was isolated from water from a cooling tower.

Gram-negative rod-shaped genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 396864

DSM 24804

@ref 20215

Last LPSN update 2026-05-29 08:26:29

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Legionellales

Family Legionellaceae

Genus Legionella

Species Legionella massiliensis

Full scientific name Legionella massiliensis Campocasso et al. 2012

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Confidence
30516	negative	2 µm	0.66 µm	rod-shaped
125438	negative				98.5
125439	negative				95.298

Colony morphology

17777

Incubation period2-3 days

Pigmentation

30516

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
17777	BCYE-AGAR (DSMZ Medium 585)		Medium recipe at MediaDive	Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base

Culture temp

@ref	Growth	Type	Temperature (°C)
17777	positive	growth	30
30516	positive	growth	28-32

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	94.565

Spore formation

@ref	Spore formation	Confidence
125438		93.446
125439		99.888

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	formaldehyde oxidation	100	3 of 3
66794	acetate fermentation	100	4 of 4
66794	coenzyme A metabolism	100	4 of 4
66794	acetoin degradation	100	3 of 3
66794	cardiolipin biosynthesis	100	7 of 7
66794	ppGpp biosynthesis	100	4 of 4
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	ubiquinone biosynthesis	100	7 of 7
66794	adipate degradation	100	2 of 2
66794	threonine metabolism	100	10 of 10
66794	suberin monomers biosynthesis	100	2 of 2
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	folate polyglutamylation	100	1 of 1
66794	biotin biosynthesis	100	4 of 4
66794	palmitate biosynthesis	95.45	21 of 22
66794	phenylalanine metabolism	92.31	12 of 13
66794	NAD metabolism	88.89	16 of 18
66794	lipid A biosynthesis	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	CO2 fixation in Crenarchaeota	88.89	8 of 9
66794	vitamin B1 metabolism	84.62	11 of 13
66794	leucine metabolism	84.62	11 of 13
66794	vitamin B6 metabolism	81.82	9 of 11
66794	proline metabolism	81.82	9 of 11
66794	peptidoglycan biosynthesis	80	12 of 15
66794	glycine betaine biosynthesis	80	4 of 5
66794	Entner Doudoroff pathway	80	8 of 10
66794	propionate fermentation	80	8 of 10
66794	ethylmalonyl-CoA pathway	80	4 of 5
66794	cellulose degradation	80	4 of 5
66794	tetrahydrofolate metabolism	78.57	11 of 14
66794	serine metabolism	77.78	7 of 9
66794	valine metabolism	77.78	7 of 9
66794	aspartate and asparagine metabolism	77.78	7 of 9
66794	alanine metabolism	75.86	22 of 29
66794	CMP-KDO biosynthesis	75	3 of 4
66794	butanoate fermentation	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	sulfopterin metabolism	75	3 of 4
66794	glycogen biosynthesis	75	3 of 4
66794	gluconeogenesis	75	6 of 8
66794	flavin biosynthesis	73.33	11 of 15
66794	purine metabolism	72.34	68 of 94
66794	photosynthesis	71.43	10 of 14
66794	citric acid cycle	71.43	10 of 14
66794	glutamate and glutamine metabolism	67.86	19 of 28
66794	lipid metabolism	67.74	21 of 31
66794	acetyl CoA biosynthesis	66.67	2 of 3
66794	cyanate degradation	66.67	2 of 3
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	lysine metabolism	66.67	28 of 42
66794	d-mannose degradation	66.67	6 of 9
66794	octane oxidation	66.67	2 of 3
66794	pyrimidine metabolism	64.44	29 of 45
66794	glutathione metabolism	64.29	9 of 14
66794	isoleucine metabolism	62.5	5 of 8
66794	C4 and CAM-carbon fixation	62.5	5 of 8
66794	ketogluconate metabolism	62.5	5 of 8
66794	histidine metabolism	62.07	18 of 29
66794	3-chlorocatechol degradation	60	3 of 5
66794	vitamin K metabolism	60	3 of 5
66794	phenylacetate degradation (aerobic)	60	3 of 5
66794	myo-inositol biosynthesis	60	6 of 10
66794	starch degradation	60	6 of 10
66794	lipoate biosynthesis	60	3 of 5
66794	glycolysis	58.82	10 of 17
66794	arginine metabolism	58.33	14 of 24
66794	tryptophan metabolism	57.89	22 of 38
66794	non-pathway related	57.89	22 of 38
66794	tyrosine metabolism	57.14	8 of 14
66794	reductive acetyl coenzyme A pathway	57.14	4 of 7
66794	propanol degradation	57.14	4 of 7
66794	polyamine pathway	56.52	13 of 23
66794	degradation of sugar alcohols	56.25	9 of 16
66794	oxidative phosphorylation	56.04	51 of 91
66794	cholesterol biosynthesis	54.55	6 of 11
66794	pentose phosphate pathway	54.55	6 of 11
66794	methionine metabolism	53.85	14 of 26
66794	pantothenate biosynthesis	50	3 of 6
66794	dTDPLrhamnose biosynthesis	50	4 of 8
66794	degradation of aromatic, nitrogen containing compounds	50	6 of 12
66794	heme metabolism	50	7 of 14
66794	phenylmercury acetate degradation	50	1 of 2
66794	selenocysteine biosynthesis	50	3 of 6
66794	ascorbate metabolism	50	11 of 22
66794	cis-vaccenate biosynthesis	50	1 of 2
66794	kanosamine biosynthesis II	50	1 of 2
66794	dolichol and dolichyl phosphate biosynthesis	50	1 of 2
66794	cysteine metabolism	50	9 of 18
66794	quinate degradation	50	1 of 2
66794	catecholamine biosynthesis	50	2 of 4
66794	vitamin E metabolism	50	2 of 4
66794	ethanol fermentation	50	1 of 2
66794	3-phenylpropionate degradation	46.67	7 of 15
66794	phosphatidylethanolamine bioynthesis	46.15	6 of 13
66794	mevalonate metabolism	42.86	3 of 7
66794	phenol degradation	40	8 of 20
66794	methylglyoxal degradation	40	2 of 5
66794	creatinine degradation	40	2 of 5
66794	glycine metabolism	40	4 of 10
66794	elloramycin biosynthesis	40	2 of 5
66794	degradation of hexoses	38.89	7 of 18
66794	isoprenoid biosynthesis	38.46	10 of 26
66794	urea cycle	38.46	5 of 13
66794	phenylpropanoid biosynthesis	38.46	5 of 13
66794	androgen and estrogen metabolism	37.5	6 of 16
66794	carnitine metabolism	37.5	3 of 8
66794	metabolism of disaccharids	36.36	4 of 11
66794	carotenoid biosynthesis	36.36	8 of 22
66794	methane metabolism	33.33	1 of 3
66794	glycolate and glyoxylate degradation	33.33	2 of 6
66794	sphingosine metabolism	33.33	2 of 6
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	arachidonic acid metabolism	33.33	6 of 18
66794	enterobactin biosynthesis	33.33	1 of 3
66794	IAA biosynthesis	33.33	1 of 3
66794	degradation of pentoses	32.14	9 of 28
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	d-xylose degradation	27.27	3 of 11
66794	lactate fermentation	25	1 of 4
66794	cyclohexanol degradation	25	1 of 4
66794	toluene degradation	25	1 of 4
66794	alginate biosynthesis	25	1 of 4
66794	bile acid biosynthesis, neutral pathway	23.53	4 of 17
66794	nitrate assimilation	22.22	2 of 9

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Industrial	#Cooling tower
#Environmental	#Aquatic	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
17777	water from a cooling tower	Bouches du Rhone	France	FRA	Europe

Taxonmaps

69479

Global distribution of 16S sequence JF779685 (>99% sequence identity) for Legionella massiliensis subclade from Microbeatlas

Aquatic Samples	279
Soil Samples	241
Animal Samples	48
Plant Samples	8
Total Samples	576

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
17777	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	PRJEB6598_assembly_1 assembly for Legionella massiliensis LegA	contig	GCA_000756815	1034943.4	2627853956	1034943	74.59

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
17777	Legionella massiliensis strain LegA 16S ribosomal RNA gene, partial sequence	JF779685	1512		1034943

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: PRJEB6598_assembly_1 assembly for Legionella massiliensis LegA
NCBI: GCA_000756815

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	94.57	no
125439	gram_stain	BacteriaNetⓘ	negative	95.30	no
125439	motility	BacteriaNetⓘ	yes	52.76	no
125439	spore_formation	BacteriaNetⓘ	no	99.89	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Legionella massiliensis LegA
PATRIC: 1034943.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	98.50	yes
125438	anaerobic	anaerobicⓘ	no	95.65	no
125438	spore-forming	spore-formingⓘ	no	93.45	no
125438	aerobic	aerobicⓘ	no	63.80	no
125438	thermophilic	thermophileⓘ	no	93.91	no
125438	flagellated	motile2+ⓘ	no	54.20	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Legionella tunisiensis sp. nov. and Legionella massiliensis sp. nov., isolated from environmental water samples.	Campocasso A, Boughalmi M, Fournous G, Raoult D, La Scola B	Int J Syst Evol Microbiol	10.1099/ijs.0.037853-0	2012

References

#17777	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 24804
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#26848	IJSEM 3003 2012 ( DOI 10.1099/ijs.0.037853-0 , PubMed 22307511 )
#30516	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26848
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Legionella massiliensis (DSM 24804, CSUR P146, LegA)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Pathway annotation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000756815

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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