Name: Legionella cardiaca H63
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6787

Type strain:

Species: Legionella cardiaca

Strain Designation: H63

Culture col. no.: DSM 25049, ATCC BAA-2315, JCM 17854

Strain history: N. Cianciotto H63 <-- Northwestern Memorial Hosp., Chicago, IL, USA.

NCBI tax ID(s): 1071983 (species)

SI-ID 401902

DSM 25049

Section

Legionella cardiaca H63 is a mesophilic, Gram-negative, motile bacterium that was isolated from aortic valve tissue of a patient with native valve endocarditis.

Gram-negative
motile
rod-shaped
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Legionellales*
	Family	*Legionellaceae*
	Genus	*Legionella*
	Species	**Legionella cardiaca**
	Full Scientific Name (LPSN)	Legionella cardiaca Pearce et al. 2012

Information on morphological and physiological properties Morphology

[Ref.: #30583]	Gram stain	negative

[Ref.: #30583]	Cell shape	rod-shaped

[Ref.: #30583]	Motility	yes

[Ref.: #17825]

Incubation period

3-7 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #17825]

Culture medium

BCYE-AGAR (DSMZ Medium 585)

[Ref.: #17825]

Culture medium growth

[Ref.: #17825]

Culture medium link

Medium recipe at MediaDive

[Ref.: #17825]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #17825]	growth	37 °C
[Ref.: #30583]	growth	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #17825]	Temperature range	mesophilic
[Ref.: #30583]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68377]	D-fructose ChEBI	-	builds acid from	* from API NH
[Ref.: #68377]	maltose ChEBI	-	builds acid from	* from API NH
[Ref.: #68377]	ornithine ChEBI	-	degradation	* from API NH
[Ref.: #68377]	sucrose ChEBI	-	builds acid from	* from API NH
[Ref.: #68377]	tryptophan ChEBI	-	energy source	* from API NH
[Ref.: #68377]	urea ChEBI	-	hydrolysis	* from API NH

	Metabolite production	Metabolite	Production
[Ref.: #68377]		indole ChEBI	no	* from API NH

	Physiological tests	Metabolite	Indole test
[Ref.: #68377]		indole ChEBI	-	* from API NH

	Enzyme	Enzyme activity	EC number
[Ref.: #68377]	beta-galactosidase	-	3.2.1.23	* from API NH
[Ref.: #68377]	beta-lactamase	-	3.5.2.6	* from API NH
[Ref.: #17825]	catalase	+	1.11.1.6
[Ref.: #30583]	catalase	+	1.11.1.6
[Ref.: #30583]	cytochrome oxidase	+	1.9.3.1
[Ref.: #17825]	cytochrome-c oxidase	-	1.9.3.1
[Ref.: #30583]	gelatinase	+
[Ref.: #68377]	ornithine decarboxylase	-	4.1.1.17	* from API NH
[Ref.: #68377]	tryptophan deaminase	-	4.1.99.1	* from API NH
[Ref.: #68377]	urease	-	3.5.1.5	* from API NH

API® NH:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Penicillinase productionPEN	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from maltoseMAL	Acid from sucroseSAC	Ornithine decarboxylaseODC	Urease/urea hydrolysisURE	LipaseLIP	Alkaline phosphatasePAL	beta-Galactosidasebeta GAL	Proline arylamidaseProA	gamma-Glutamyl transferaseGGT	Indole productionIND
[Ref.: #17825]	6227	-	-	-	-	-	-	-	+	-	-	+	-	-
[Ref.: #17825]	5965	-	+/-	-	-	-	-	-	-	+	-	-	+/-	-
[Ref.: #17825]	5645	-	-	-	-	-	-	-	-	+	-	-	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #17825]	Sample type/isolated from	aortic valve tissue of a patient with native valve endocarditis
[Ref.: #17825]	Geographic location (country and/or sea, region)	IL, Chicago
[Ref.: #17825]	Country	USA
[Ref.: #17825]	Country ISO 3 Code	USA
[Ref.: #17825]	Continent	North America
[Ref.: #17825]	Geographic location	41.8818°/-87.6232°

[Ref.: #67770]	Sample type/isolated from	Human aortic valve tissue and the causative agent of endocarditis
* marker position based on {}

Isolation sources categories

#Infection	#Patient	-
#Host Body-Site	#Organ	#Heart

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence JF831047 (>99% sequence identity) for Legionella cardiaca from Microbeatlas

Aquatic Samples	96
Soil Samples	23
Animal Samples	16
Plant Samples	10
Total Samples	145

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #17825]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #17825]	Legionella cardiaca strain H63 16S ribosomal RNA gene, partial sequence	JF831047	1423	ENA	1071983 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Legionella cardiaca H63	GCA_029026145	complete	GenBank	1071983 tax ID	*

[Ref.: #17825]	GC-content	41.8 mol%	high performance liquid chromatography (HPLC)
[Ref.: #30583]	GC-content	41.8 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Legionella cardiaca H63 NCBI: GCA_029026145.1
[Ref.: #69480]	motile	no	85.124	no
[Ref.: #69480]	flagellated	no	92.467	no
[Ref.: #69480]	gram-positive	no	97.62	yes
[Ref.: #69480]	anaerobic	no	84.277	no
[Ref.: #69480]	aerobic	no	80.276	no
[Ref.: #69480]	halophile	no	72.785	no
[Ref.: #69480]	spore-forming	no	97.047	no
[Ref.: #69480]	glucose-ferment	yes	70.319	no
[Ref.: #69480]	thermophile	no	98.095	yes
[Ref.: #69480]	glucose-util	yes	80.553	no

Availability in culture collections External links

[Ref.: #17825]

Culture collection no.

DSM 25049, ATCC BAA-2315, JCM 17854

[Ref.: #76257]

SI-ID 401902

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Legionella cardiaca sp. nov., isolated from a case of native valve endocarditis in a human heart.	Pearce MM, Theodoropoulos N, Mandel MJ, Brown E, Reed KD, Cianciotto NP	Int J Syst Evol Microbiol	10.1099/ijs.0.039248-0	2012	*

References

#17825

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 25049

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30583

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26914 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68377

Automatically annotated from API NH .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#76257

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID401902.1 )

#26914

IJSEM 2946 2012 ( DOI 10.1099/ijs.0.039248-0 , PubMed 22286905 )

* These data were automatically processed and therefore are not curated

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