Legionella waltersii (DSM 21908, ATCC 51914, CCUG 44924)

Name: Legionella waltersii (DSM 21908, ATCC 51914, CCUG 44924)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6766

Type strain

Strain Designation 2074-AUS-E

Culture col. no. DSM 21908 ATCC 51914 CCUG 44924 CIP 104965 2074-AUS-E

NCBI tax ID(s) 66969

Special Collections DSMZ CCUG CIP

History DSMZ <- CCUG <- CIP <- R. F. Benson, CDC; 2074-AUS-E <- R. P. Walters CIP <- 1996, R.F. Benson, CDC: strain 2074-AUS-E <- R.P. Walters

Links

Legionella waltersii 2074-AUS-E is a microaerophile, Gram-negative, rod-shaped bacterium that was isolated from drinking water distribution system.

Gram-negative rod-shaped microaerophile genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 361673

NCTC 13017,ATCC 51914,CIP 104965,CCUG 44924,DSM 21908

@ref 20215

Last LPSN update 2026-05-29 08:26:29

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Legionellales

Family Legionellaceae

Genus Legionella

Species Legionella waltersii

Full scientific name Legionella waltersii Benson et al. 1996

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
123904	negative	rod-shaped
125438	negative		97.979
125439	negative		96.674

Colony morphology

15903

Incubation period3-7 days

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
15903	BCYE-AGAR (DSMZ Medium 585)	Medium recipe at MediaDive	Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
39149	MEDIUM 23 - for Afipia and Legionella		Distilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
123904	CIP Medium 23	Medium recipe at CIP
123904	CIP Medium 748	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
15903	positive	growth	30
39149	positive	growth	37
56546	positive	growth	37
123904	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
15903	microaerophile
56546	microaerophile
125439	obligate aerobe	97.616

Spore formation

@ref	Spore formation	Confidence
125439		99.735

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
15903	catalase	+	1.11.1.6
123904	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
15903	cytochrome-c oxidase	-	1.9.3.1
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	+		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
123904	oxidase	+
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	-		from API zym

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	suberin monomers biosynthesis	100	2 of 2
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	adipate degradation	100	2 of 2
66794	folate polyglutamylation	100	1 of 1
66794	biotin biosynthesis	100	4 of 4
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	ethanol fermentation	100	2 of 2
66794	ppGpp biosynthesis	100	4 of 4
66794	palmitate biosynthesis	95.45	21 of 22
66794	phenylalanine metabolism	92.31	12 of 13
66794	lipid A biosynthesis	88.89	8 of 9
66794	valine metabolism	88.89	8 of 9
66794	CO2 fixation in Crenarchaeota	88.89	8 of 9
66794	ubiquinone biosynthesis	85.71	6 of 7
66794	vitamin B1 metabolism	84.62	11 of 13
66794	Entner Doudoroff pathway	80	8 of 10
66794	glycine betaine biosynthesis	80	4 of 5
66794	threonine metabolism	80	8 of 10
66794	glycogen metabolism	80	4 of 5
66794	peptidoglycan biosynthesis	80	12 of 15
66794	cellulose degradation	80	4 of 5
66794	alanine metabolism	79.31	23 of 29
66794	glutamate and glutamine metabolism	78.57	22 of 28
66794	tetrahydrofolate metabolism	78.57	11 of 14
66794	d-mannose degradation	77.78	7 of 9
66794	chorismate metabolism	77.78	7 of 9
66794	NAD metabolism	77.78	14 of 18
66794	leucine metabolism	76.92	10 of 13
66794	CMP-KDO biosynthesis	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	acetate fermentation	75	3 of 4
66794	butanoate fermentation	75	3 of 4
66794	degradation of aromatic, nitrogen containing compounds	75	9 of 12
66794	sulfopterin metabolism	75	3 of 4
66794	coenzyme A metabolism	75	3 of 4
66794	lysine metabolism	73.81	31 of 42
66794	pyrimidine metabolism	73.33	33 of 45
66794	tyrosine metabolism	71.43	10 of 14
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	tryptophan metabolism	71.05	27 of 38
66794	purine metabolism	70.21	66 of 94
66794	propionate fermentation	70	7 of 10
66794	serine metabolism	66.67	6 of 9
66794	IAA biosynthesis	66.67	2 of 3
66794	formaldehyde oxidation	66.67	2 of 3
66794	octane oxidation	66.67	2 of 3
66794	cyanate degradation	66.67	2 of 3
66794	aspartate and asparagine metabolism	66.67	6 of 9
66794	acetoin degradation	66.67	2 of 3
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	photosynthesis	64.29	9 of 14
66794	citric acid cycle	64.29	9 of 14
66794	heme metabolism	64.29	9 of 14
66794	proline metabolism	63.64	7 of 11
66794	vitamin B6 metabolism	63.64	7 of 11
66794	gluconeogenesis	62.5	5 of 8
66794	C4 and CAM-carbon fixation	62.5	5 of 8
66794	ketogluconate metabolism	62.5	5 of 8
66794	histidine metabolism	62.07	18 of 29
66794	myo-inositol biosynthesis	60	6 of 10
66794	metabolism of amino sugars and derivatives	60	3 of 5
66794	methylglyoxal degradation	60	3 of 5
66794	flavin biosynthesis	60	9 of 15
66794	glycolysis	58.82	10 of 17
66794	non-pathway related	57.89	22 of 38
66794	glutathione metabolism	57.14	8 of 14
66794	propanol degradation	57.14	4 of 7
66794	reductive acetyl coenzyme A pathway	57.14	4 of 7
66794	lipid metabolism	54.84	17 of 31
66794	pentose phosphate pathway	54.55	6 of 11
66794	arginine metabolism	54.17	13 of 24
66794	phosphatidylethanolamine bioynthesis	53.85	7 of 13
66794	polyamine pathway	52.17	12 of 23
66794	oxidative phosphorylation	51.65	47 of 91
66794	kanosamine biosynthesis II	50	1 of 2
66794	cis-vaccenate biosynthesis	50	1 of 2
66794	isoleucine metabolism	50	4 of 8
66794	pantothenate biosynthesis	50	3 of 6
66794	cyclohexanol degradation	50	2 of 4
66794	quinate degradation	50	1 of 2
66794	phenylmercury acetate degradation	50	1 of 2
66794	selenocysteine biosynthesis	50	3 of 6
66794	glycogen biosynthesis	50	2 of 4
66794	methionine metabolism	46.15	12 of 26
66794	ascorbate metabolism	45.45	10 of 22
66794	cysteine metabolism	44.44	8 of 18
66794	degradation of sugar alcohols	43.75	7 of 16
66794	benzoyl-CoA degradation	42.86	3 of 7
66794	mevalonate metabolism	42.86	3 of 7
66794	3-phenylpropionate degradation	40	6 of 15
66794	lipoate biosynthesis	40	2 of 5
66794	bacilysin biosynthesis	40	2 of 5
66794	glycine metabolism	40	4 of 10
66794	isoprenoid biosynthesis	38.46	10 of 26
66794	urea cycle	38.46	5 of 13
66794	phenylpropanoid biosynthesis	38.46	5 of 13
66794	androgen and estrogen metabolism	37.5	6 of 16
66794	dTDPLrhamnose biosynthesis	37.5	3 of 8
66794	degradation of pentoses	35.71	10 of 28
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	methane metabolism	33.33	1 of 3
66794	glycolate and glyoxylate degradation	33.33	2 of 6
66794	enterobactin biosynthesis	33.33	1 of 3
66794	arachidonic acid metabolism	33.33	6 of 18
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	sphingosine metabolism	33.33	2 of 6
66794	bile acid biosynthesis, neutral pathway	29.41	5 of 17
66794	degradation of hexoses	27.78	5 of 18
66794	d-xylose degradation	27.27	3 of 11
66794	metabolism of disaccharids	27.27	3 of 11
66794	alginate biosynthesis	25	1 of 4
66794	toluene degradation	25	1 of 4
66794	catecholamine biosynthesis	25	1 of 4
66794	lactate fermentation	25	1 of 4
66794	nitrate assimilation	22.22	2 of 9
66794	4-hydroxymandelate degradation	22.22	2 of 9

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
123904	-	+	-	+	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Freshwater

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
15903	drinking water distribution system	Adelaide	Australia	AUS	Australia and Oceania
56546	Drinking water distribution system	Adelaide	Australia	AUS	Australia and Oceania
123904	Environment, Drinking water distribution system	Adelaide	Australia	AUS	Australia and Oceania

Taxonmaps

69479

Global distribution of 16S sequence AF122886 (>99% sequence identity) for Legionella waltersii from Microbeatlas

Aquatic Samples	141
Soil Samples	96
Animal Samples	45
Plant Samples	23
Total Samples	305

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
15903	1	Risk group (German classification) According to TRBA 466
123904	2	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	Leg_waltersii_2074-AUS-E.v1 assembly for Legionella waltersii 2074-AUS-E	contig	GCA_900640055	66969.8	8110540826	66969	62.17
66792	ASM146808v1 assembly for Legionella waltersii ATCC 51914	scaffold	GCA_001468085	66969.6	2663762636	66969	51.97

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
15903	Legionella waltersii strain 2074-AUS-E 16S ribosomal RNA gene, partial sequence	AF122886	1447		66969

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Leg_waltersii_2074-AUS-E.v1 assembly for Legionella waltersii 2074-AUS-E
NCBI: GCA_900640055

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	97.62	no
125439	gram_stain	BacteriaNetⓘ	negative	96.67	no
125439	motility	BacteriaNetⓘ	no	68.95	no
125439	spore_formation	BacteriaNetⓘ	no	99.74	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Legionella waltersii ATCC 51914
NCBI: GCA_001468085.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	97.98	no
125438	anaerobic	anaerobicⓘ	no	97.09	yes
125438	aerobic	aerobicⓘ	no	67.39	yes
125438	spore-forming	spore-formingⓘ	no	89.99	no
125438	thermophilic	thermophileⓘ	no	92.47	yes
125438	flagellated	motile2+ⓘ	no	59.44	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Validation of MICA Legionella for Enumeration of Legionella pneumophila in Sanitary Waters and Cooling Tower Waters: AOAC Performance Tested MethodSM 032201.	Passot F, Peslier S, Benzinger MJ, Blackburn J, Thompson W, Bastin B, Dumont A, Dukan S.	J AOAC Int	10.1093/jaoacint/qsac150	2023
Enzymology	Development and evaluation of Chlamylege, a new commercial test allowing simultaneous detection and identification of Legionella, Chlamydophila pneumoniae, and Mycoplasma pneumoniae in clinical respiratory specimens by multiplex PCR.	Ginevra C, Barranger C, Ros A, Mory O, Stephan JL, Freymuth F, Joannes M, Pozzetto B, Grattard F.	J Clin Microbiol	10.1128/jcm.43.7.3247-3254.2005	2005
Phylogeny	Legionella waltersii sp. nov. and an unnamed Legionella genomospecies isolated from water in Australia.	Benson RF, Thacker WL, Daneshvar MI, Brenner DJ	Int J Syst Bacteriol	10.1099/00207713-46-3-631	1996

References

#15903	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21908
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#39149	; Curators of the CIP;
#56546	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 44924
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#123904	Collection of Institut Pasteur ; Curators of the CIP; CIP 104965
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

About us

Legionella waltersii (DSM 21908, ATCC 51914, CCUG 44924)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Enzymes

Pathway annotation

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_900640055

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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