Legionella worsleiensis (DSM 21907, ATCC 49508, CCUG 44923)

Name: Legionella worsleiensis (DSM 21907, ATCC 49508, CCUG 44923)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6764

Type strain

Strain Designation 1347, 95/83-1347

Culture col. no. DSM 21907 ATCC 49508 CCUG 44923 CIP 105114 NCTC 12377 2 more

NCBI tax ID(s) 45076

Special Collections DSMZ CCUG CIP

History DSMZ <- CCUG <- CIP <- NCTC <- T. Harrison, Lab. Microbiol. Reagents (LMR), Central Public Health Lab., Colindale, London, UK <- P. J. Dennis, PHLS Salisbury, UK; 95/83-1347 CIP <- 1997, NCTC <- 1993, T. Harrison, LMR Colindale <- P.J. Dennis: strain 95/83-1347

Links

Legionella worsleiensis 1347 is a microaerophile, Gram-negative, rod-shaped bacterium that was isolated from cooling tower return flow.

Gram-negative rod-shaped microaerophile genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 361674

ATCC 49508,CIP 105114,NCTC 12377,CCUG 44923,DSM 21907

@ref 20215

Last LPSN update 2026-05-29 08:26:29

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Legionellales

Family Legionellaceae

Genus Legionella

Species Legionella worsleiensis

Full scientific name Legionella worsleiensis Dennis et al. 1993

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
123961	negative	rod-shaped
125439	negative		98.759
125438	negative		98.296

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
15902	BCYE-AGAR (DSMZ Medium 585)	Medium recipe at MediaDive	Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
35175	MEDIUM 23 - for Afipia and Legionella		Distilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
123961	CIP Medium 23	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
15902	positive	growth	37
35175	positive	growth	37
56545	positive	growth	37
123961	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
15902	microaerophile
56545	microaerophile
125439	obligate aerobe	90.76

Spore formation

@ref	Spore formation	Confidence
125439		99.857
125438		91.368

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
123961	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
123961	oxidase	+
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	+		from API zym

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	biotin biosynthesis	100	4 of 4
66794	cardiolipin biosynthesis	100	7 of 7
66794	palmitate biosynthesis	100	22 of 22
66794	kanosamine biosynthesis II	100	2 of 2
66794	adipate degradation	100	2 of 2
66794	formaldehyde oxidation	100	3 of 3
66794	ubiquinone biosynthesis	100	7 of 7
66794	coenzyme A metabolism	100	4 of 4
66794	acetoin degradation	100	3 of 3
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	suberin monomers biosynthesis	100	2 of 2
66794	folate polyglutamylation	100	1 of 1
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	threonine metabolism	90	9 of 10
66794	chorismate metabolism	88.89	8 of 9
66794	NAD metabolism	83.33	15 of 18
66794	vitamin B6 metabolism	81.82	9 of 11
66794	Entner Doudoroff pathway	80	8 of 10
66794	hydrogen production	80	4 of 5
66794	peptidoglycan biosynthesis	80	12 of 15
66794	glycine betaine biosynthesis	80	4 of 5
66794	CO2 fixation in Crenarchaeota	77.78	7 of 9
66794	valine metabolism	77.78	7 of 9
66794	lipid A biosynthesis	77.78	7 of 9
66794	leucine metabolism	76.92	10 of 13
66794	phenylalanine metabolism	76.92	10 of 13
66794	vitamin B1 metabolism	76.92	10 of 13
66794	alanine metabolism	75.86	22 of 29
66794	ppGpp biosynthesis	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	butanoate fermentation	75	3 of 4
66794	glycogen biosynthesis	75	3 of 4
66794	acetate fermentation	75	3 of 4
66794	sulfopterin metabolism	75	3 of 4
66794	CMP-KDO biosynthesis	75	3 of 4
66794	flavin biosynthesis	73.33	11 of 15
66794	lysine metabolism	71.43	30 of 42
66794	tetrahydrofolate metabolism	71.43	10 of 14
66794	pyrimidine metabolism	68.89	31 of 45
66794	(5R)-carbapenem carboxylate biosynthesis	66.67	2 of 3
66794	d-mannose degradation	66.67	6 of 9
66794	serine metabolism	66.67	6 of 9
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	octane oxidation	66.67	2 of 3
66794	aspartate and asparagine metabolism	66.67	6 of 9
66794	glycolysis	64.71	11 of 17
66794	glutathione metabolism	64.29	9 of 14
66794	photosynthesis	64.29	9 of 14
66794	glutamate and glutamine metabolism	64.29	18 of 28
66794	proline metabolism	63.64	7 of 11
66794	pentose phosphate pathway	63.64	7 of 11
66794	purine metabolism	62.77	59 of 94
66794	C4 and CAM-carbon fixation	62.5	5 of 8
66794	gluconeogenesis	62.5	5 of 8
66794	isoleucine metabolism	62.5	5 of 8
66794	propionate fermentation	60	6 of 10
66794	lipoate biosynthesis	60	3 of 5
66794	methylglyoxal degradation	60	3 of 5
66794	starch degradation	60	6 of 10
66794	lipid metabolism	58.06	18 of 31
66794	citric acid cycle	57.14	8 of 14
66794	tryptophan metabolism	55.26	21 of 38
66794	3-phenylpropionate degradation	53.33	8 of 15
66794	non-pathway related	52.63	20 of 38
66794	degradation of aromatic, nitrogen containing compounds	50	6 of 12
66794	phenylmercury acetate degradation	50	1 of 2
66794	ribulose monophosphate pathway	50	1 of 2
66794	ethanol fermentation	50	1 of 2
66794	dTDPLrhamnose biosynthesis	50	4 of 8
66794	heme metabolism	50	7 of 14
66794	tyrosine metabolism	50	7 of 14
66794	selenocysteine biosynthesis	50	3 of 6
66794	ketogluconate metabolism	50	4 of 8
66794	quinate degradation	50	1 of 2
66794	catecholamine biosynthesis	50	2 of 4
66794	urea cycle	46.15	6 of 13
66794	histidine metabolism	44.83	13 of 29
66794	cysteine metabolism	44.44	8 of 18
66794	propanol degradation	42.86	3 of 7
66794	methionine metabolism	42.31	11 of 26
66794	bile acid biosynthesis, neutral pathway	41.18	7 of 17
66794	carotenoid biosynthesis	40.91	9 of 22
66794	D-cycloserine biosynthesis	40	2 of 5
66794	4-hydroxyphenylacetate degradation	40	4 of 10
66794	myo-inositol biosynthesis	40	4 of 10
66794	arachidonate biosynthesis	40	2 of 5
66794	vitamin K metabolism	40	2 of 5
66794	glycine metabolism	40	4 of 10
66794	gallate degradation	40	2 of 5
66794	glycogen metabolism	40	2 of 5
66794	polyamine pathway	39.13	9 of 23
66794	phosphatidylethanolamine bioynthesis	38.46	5 of 13
66794	isoprenoid biosynthesis	38.46	10 of 26
66794	degradation of sugar alcohols	37.5	6 of 16
66794	androgen and estrogen metabolism	37.5	6 of 16
66794	arginine metabolism	37.5	9 of 24
66794	oxidative phosphorylation	37.36	34 of 91
66794	phenol degradation	35	7 of 20
66794	pantothenate biosynthesis	33.33	2 of 6
66794	methane metabolism	33.33	1 of 3
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	arachidonic acid metabolism	33.33	6 of 18
66794	cyanate degradation	33.33	1 of 3
66794	IAA biosynthesis	33.33	1 of 3
66794	enterobactin biosynthesis	33.33	1 of 3
66794	glycolate and glyoxylate degradation	33.33	2 of 6
66794	sulfate reduction	30.77	4 of 13
66794	mevalonate metabolism	28.57	2 of 7
66794	reductive acetyl coenzyme A pathway	28.57	2 of 7
66794	degradation of hexoses	27.78	5 of 18
66794	d-xylose degradation	27.27	3 of 11
66794	metabolism of disaccharids	27.27	3 of 11
66794	cholesterol biosynthesis	27.27	3 of 11
66794	lactate fermentation	25	1 of 4
66794	cyclohexanol degradation	25	1 of 4
66794	toluene degradation	25	1 of 4
66794	degradation of pentoses	25	7 of 28
66794	alginate biosynthesis	25	1 of 4
66794	4-hydroxymandelate degradation	22.22	2 of 9
66794	nitrate assimilation	22.22	2 of 9

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
123961	-	+	+	+	-	+	+	-	-	-	+	+	-	-	-	-	-	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
123961	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Industrial	#Cooling tower

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
15902	cooling tower return flow	Worsley	United Kingdom	GBR	Europe
56545	Cooling tower return flow	Worsley	United Kingdom	GBR	Europe
123961	Cooling tower return flow	Worsley	United Kingdom	GBR	Europe

Taxonmaps

69479

Global distribution of 16S sequence Z49739 (>99% sequence identity) for Legionella worsleiensis from Microbeatlas

Aquatic Samples	24
Soil Samples	3
Animal Samples	3
Plant Samples	0
Total Samples	30

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
15902	1	Risk group (German classification) According to TRBA 466
123961	2	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	52465_G01 assembly for Legionella worsleiensis NCTC12377	contig	GCA_900453045	45076.7	2808606675	45076	77.19
66792	ASM146753v1 assembly for Legionella worsleiensis ATCC 49508	scaffold	GCA_001467535	45076.6	2660238685	45076	63.94

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
15902	L.worsliensis gene for ribosomal RNA, small subunit	Z49739	1415		45076
124043	Legionella worsleiensis strain MDC1604 16S ribosomal RNA gene, partial sequence.	JF720415	524		45076

GC content

15902

GC-content (mol%)41

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: 52465_G01 assembly for Legionella worsleiensis NCTC12377
NCBI: GCA_900453045

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	90.76	no
125439	gram_stain	BacteriaNetⓘ	negative	98.76	no
125439	motility	BacteriaNetⓘ	yes	47.03	no
125439	spore_formation	BacteriaNetⓘ	no	99.86	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Legionella worsleiensis ATCC 49508
NCBI: GCA_001467535.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	98.30	no
125438	anaerobic	anaerobicⓘ	no	95.44	no
125438	aerobic	aerobicⓘ	no	78.86	no
125438	spore-forming	spore-formingⓘ	no	91.37	no
125438	thermophilic	thermophileⓘ	no	91.80	yes
125438	flagellated	motile2+ⓘ	yes	56.58	no

Literature

References

#15902	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21907
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#35175	; Curators of the CIP;
#56545	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 44923
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#123961	Collection of Institut Pasteur ; Curators of the CIP; CIP 105114
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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