Name: Legionella hackeliae DSM 19214
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6748

Type strain:

Species: Legionella hackeliae

Culture col. no.: DSM 19214, ATCC 35250, CIP 103844, JCM 7563, Lansing 2, NCTC 11979, CCUG 31232

Strain history: GIFU 10740 <-- CDC, strain Lansing 2 <-- B. B. Wentworth <-- M. Hackel.

NCBI tax ID(s): 449 (species)

Other strains from Legionella hackeliae

L. hackeliae R 44, 798-PA-H, DSM 25323, ATCC ...

Section

Legionella hackeliae DSM 19214 is a microaerophile, mesophilic bacterium that was isolated from human bronchial biopsy.

mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Legionellales*
	Family	*Legionellaceae*
	Genus	*Legionella*
	Species	**Legionella hackeliae**
	Full Scientific Name (PNU)	Legionella hackeliae Brenner et al. 1985

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.999

[Ref.: #69480]	Motility	yes Confidence in %96.543

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7972]

Culture medium

BCYE-AGAR (DSMZ Medium 585)

[Ref.: #7972]

Culture medium growth

[Ref.: #7972]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #7972]

Culture medium composition

expand / minimize

[Ref.: #40810]

Culture medium

MEDIUM 23 - for Afipia and Legionella

[Ref.: #40810]

Culture medium growth

[Ref.: #40810]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #7972]	growth	37 °C
[Ref.: #40810]	growth	37 °C
[Ref.: #50487]	growth	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #7972]	Temperature range	mesophilic
[Ref.: #40810]	Temperature range	mesophilic
[Ref.: #50487]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #7972]	Oxygen tolerance	microaerophile
[Ref.: #50487]	Oxygen tolerance	microaerophile

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.993

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7972]	Sample type/isolated from	human bronchial biopsy
[Ref.: #7972]	Geographic location (country and/or sea, region)	Ann Arbor
[Ref.: #7972]	Country	USA
[Ref.: #7972]	Country ISO 3 Code	USA
[Ref.: #7972]	Continent	North America

[Ref.: #50487]	Sample type/isolated from	Human bronchial biopsy
[Ref.: #50487]	Geographic location (country and/or sea, region)	Michigan,Ann Arbor
[Ref.: #50487]	Country	USA
[Ref.: #50487]	Country ISO 3 Code	USA
[Ref.: #50487]	Continent	North America

[Ref.: #67770]	Sample type/isolated from	Bronchial biopsy, human pneumonia
[Ref.: #67770]	Geographic location (country and/or sea, region)	Ann Arbor, MI
[Ref.: #67770]	Country	USA
[Ref.: #67770]	Country ISO 3 Code	USA
[Ref.: #67770]	Continent	North America
* marker position based on {}

Isolation sources categories

#Infection	#Patient	#Biopsy
#Host	#Human	-
#Host Body-Site	#Oral cavity and airways	#Airways

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LN681225 (>99% sequence identity) for Legionella hackeliae subclade from Microbeatlas

Aquatic Samples	102
Soil Samples	13
Animal Samples	14
Plant Samples	2
Total Samples	131

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7972]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Legionella hackeliae JCM 7563 gene for 16S ribosomal RNA, partial sequence	LC504040	1467	ENA	449 tax ID
[Ref.: #67770]	Legionella hackeliae partial 16S rRNA gene, strain Lancing 2	HF558377	1518	ENA	449 tax ID
[Ref.: #7972]	Legionella hackeliae genome assembly LHA, chromosome : I	LN681225	3440975	ENA	449 tax ID
[Ref.: #67770]	Legionella hackeliae 16S rRNA sequence	M36028	1473	ENA	449 tax ID
[Ref.: #20218]	Legionella hackeliae strain MDC1585 16S ribosomal RNA gene, partial sequence	JF720375	525	ENA	449 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Legionella hackeliae genome assembly LHA, chromosome : I	LN681225		ENA	449 tax ID
[Ref.: #67770]	Legionella hackeliae NCTC11979	GCA_900452565	contig	GenBank	449 tax ID
[Ref.: #66792]	Legionella hackeliae ATCC35250	GCA_000953655	complete	GenBank	449 tax ID	*
[Ref.: #66792]	Legionella hackeliae strain ATCC35250	449.5	complete	PATRIC	449 tax ID	*
[Ref.: #66792]	Legionella hackeliae strain NCTC11979	449.8	wgs	PATRIC	449 tax ID	*
[Ref.: #66792]	Legionella hackeliae ATCC35250	2654588001	complete	JGI IMG/M	449 tax ID	*

[Ref.: #67770]

GC-content

40 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Legionella hackeliae ATCC35250 NCBI: GCA_000953655.1
[Ref.: #69480]	spore-forming	no	99.993	yes
[Ref.: #69480]	anaerobic	no	96.895	yes
[Ref.: #69480]	gram-positive	no	99.999	no
[Ref.: #69480]	thermophile	no	99.831	yes
[Ref.: #69480]	motile	yes	96.543	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Legionella hackeliae ATCC35250 NCBI: GCA_000953655.1
[Ref.: #69481]	spore-forming	no	100	no

Availability in culture collections External links

[Ref.: #7972]

Culture collection no.

DSM 19214, ATCC 35250, CIP 103844, JCM 7563, Lansing 2, NCTC 11979, CCUG 31232

[Ref.: #20218]

Associated Passport(s) in StrainInfo

718986, 120856, 152346, 183038, 120857, 120858

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Second serogroup of Legionella hackeliae isolated from a patient with pneumonia.	Wilkinson HW, Thacker WL, Steigerwalt AG, Brenner DJ, Ampel NM, Wing EJ	J Clin Microbiol	10.1128/jcm.22.4.488-489.1985	1985	*

References

#7972

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19214

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#40810 ; Curators of the CIP;
#50487 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 31232 A

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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Legionella hackeliae ATCC35250

Legionella hackeliae ATCC35250

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