Legionella hackeliae (DSM 19214, ATCC 35250, CIP 103844)

Name: Legionella hackeliae (DSM 19214, ATCC 35250, CIP 103844)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6748

Type strain

Culture col. no. DSM 19214 ATCC 35250 CIP 103844 JCM 7563 Lansing 2 2 more

NCBI tax ID(s) 449

Special Collections DSMZ CCUG JCM CIP

History DSMZ <- H. E. Müller <- ATCC <- W. F. Bibb <- B. B. Wentworth <- M. Hackel JCM <-- GIFU 10740 <-- CDC, strain Lansing 2 <-- B. B. Wentworth <-- M. Hackel. CIP <- 1993, R.F. Benson, CDC: strain Lansing 2 <- B.B. Wentworth <- M. Hackel

Links

Legionella hackeliae DSM 19214 is a microaerophile, Gram-negative, rod-shaped bacterium that was isolated from human bronchial biopsy.

Gram-negative rod-shaped microaerophile genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 125847

ATCC 35250,NCTC 11979,CCUG 31232 A,CIP 103844,JCM 7563,DSM 19214

@ref 20215

Last LPSN update 2026-05-29 08:26:29

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Legionellales

Family Legionellaceae

Genus Legionella

Species Legionella hackeliae

Full scientific name Legionella hackeliae Brenner et al. 1985

BacDive ID	Other strains from **Legionella hackeliae** (1)	Type strain
6749	L. hackeliae R 44, 798-PA-H, DSM 25323, ATCC 35999, NCTC ...

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility	Confidence
119759	negative	rod-shaped
125438	negative			98.994

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
7972	BCYE-AGAR (DSMZ Medium 585)	Medium recipe at MediaDive	Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
40810	MEDIUM 23 - for Afipia and Legionella		Distilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
119759	CIP Medium 23	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
7972	positive	growth	37
40810	positive	growth	37
50487	positive	growth	37
67770	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
7972	microaerophile
50487	microaerophile
125439	obligate aerobe	98.39

Spore formation

@ref	Spore formation	Confidence
125439		96.96

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
119759	17632 ChEBI	nitrate	-	reduction
119759	16301 ChEBI	nitrite	-	reduction

Metabolite production

@ref	Chebi-ID	Metabolite	Production
119759	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
119759	catalase	+	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	-		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
119759	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
119759	urease	-	3.5.1.5
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
119759	-	+	-	-	-	+	+	+	-	-	+	+	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Infection	#Patient	#Biopsy
#Host	#Human	-
#Host Body-Site	#Oral cavity and airways	#Airways

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent
7972	human bronchial biopsy	Homo sapiens	Ann Arbor	USA	USA	North America
50487	Human bronchial biopsy	Homo sapiens	Michigan,Ann Arbor	USA	USA	North America
67770	Bronchial biopsy, human pneumonia	Homo sapiens	Ann Arbor, MI	USA	USA	North America
119759	Human, Bronchial biopsy	Homo sapiens	Ann Arbor, Michigan	United States of America	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence LN681225 (>99% sequence identity) for Legionella hackeliae subclade from Microbeatlas

Aquatic Samples	102
Soil Samples	13
Animal Samples	14
Plant Samples	2
Total Samples	131

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
7972	2	Risk group (German classification) According to TRBA 466
119759	2	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	LHA assembly for Legionella hackeliae ATCC35250	complete	GCA_000953655	449.5	2654588001	449	98.94
67770	34347_D01 assembly for Legionella hackeliae NCTC11979	contig	GCA_900452565	449.8	8117540350	449	74.52

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Legionella hackeliae strain MDC1585 16S ribosomal RNA gene, partial sequence	JF720375	525	449
67770	Legionella hackeliae partial 16S rRNA gene, strain Lancing 2	HF558377	1518	449
67770	Legionella hackeliae JCM 7563 gene for 16S ribosomal RNA, partial sequence	LC504040	1467	449
67770	Legionella hackeliae 16S rRNA sequence	M36028	1473	449

GC content

@ref	GC-content (mol%)	Method
67770	40	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: LHA assembly for Legionella hackeliae ATCC35250
BV-BRC: 449.5

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.39	no
125439	gram_stain	BacteriaNetⓘ	negative	86.18	no
125439	motility	BacteriaNetⓘ	no	59.80	no
125439	spore_formation	BacteriaNetⓘ	no	96.96	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Legionella hackeliae ATCC35250
NCBI: GCA_000953655.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	98.99	no
125438	anaerobic	anaerobicⓘ	no	95.76	no
125438	aerobic	aerobicⓘ	no	73.64	yes
125438	spore-forming	spore-formingⓘ	no	89.18	no
125438	thermophilic	thermophileⓘ	no	95.49	no
125438	flagellated	motile2+ⓘ	no	58.41	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Draft Genome Sequence of Legionella Species Isolated from Drinking Water in an Italian Industry.	Girolamini L, Salaris S, Orsini M, Pascale MR, Mazzotta M, Grottola A, Cristino S.	Microbiol Resour Announc	10.1128/mra.01152-21	2022
Enzymology	Development and evaluation of Chlamylege, a new commercial test allowing simultaneous detection and identification of Legionella, Chlamydophila pneumoniae, and Mycoplasma pneumoniae in clinical respiratory specimens by multiplex PCR.	Ginevra C, Barranger C, Ros A, Mory O, Stephan JL, Freymuth F, Joannes M, Pozzetto B, Grattard F.	J Clin Microbiol	10.1128/jcm.43.7.3247-3254.2005	2005
Phylogeny	Isolation of Legionella oakridgensis from two patients with pleural effusion living in the same geographical area.	Lo Presti F, Riffard S, Jarraud S, Le Gallou F, Richet H, Vandenesch F, Etienne J.	J Clin Microbiol	10.1128/jcm.38.8.3128-3130.2000	2000
Pathogenicity	Susceptibility of Legionella strains to the chlorinated biocide, monochloramine.	Jakubek D, Guillaume C, Binet M, Leblon G, DuBow M, Le Brun M.	Microbes Environ	10.1264/jsme2.me12205	2013
	Genus-specific epitope on the 60-kilodalton Legionella heat shock protein recognized by a monoclonal antibody.	Steinmetz I, Rheinheimer C, Hubner I, Bitter-Suermann D.	J Clin Microbiol	10.1128/jcm.29.2.346-354.1991	1991
	An acetyltransferase effector conserved across Legionella species targets the eukaryotic eIF3 complex to modulate protein translation.	Syriste L, Patel DT, Stogios PJ, Skarina T, Patel D, Savchenko A.	mBio	10.1128/mbio.03221-23	2024
	Evaluation of five Legionella urinary antigen detection kits including new Ribotest Legionella for simultaneous detection of ribosomal protein L7/L12.	Nakamura A, Fukuda S, Kusuki M, Watari H, Shimura S, Kimura K, Nishi I, Komatsu M, Study of Bacterial Resistance in the Kinki region of japan (SBRK) group.	J Infect Chemother	10.1016/j.jiac.2021.05.019	2021
Genetics	Comparative analysis of Legionella lytica genome identifies specific metabolic traits and virulence factors.	Koper P, Wysokinski J, Zebracki K, Decewicz P, Dziewit L, Kalita M, Palusinska-Szysz M, Mazur A.	Sci Rep	10.1038/s41598-025-90154-5	2025
	Novel induction of broad-spectrum antibiotics by the human pathogen Legionella.	Shin CJ, O'Connor TJ.	mSphere	10.1128/msphere.00120-24	2024
Metabolism	Brain Meta-Transcriptomics from Harbor Seals to Infer the Role of the Microbiome and Virome in a Stranding Event.	Rosales SM, Thurber RV.	PLoS One	10.1371/journal.pone.0143944	2015
	Genome Sequence and Characterization of a Xanthorhodopsin-Containing, Aerobic Anoxygenic Phototrophic Rhodobacter Species, Isolated from Mesophilic Conditions at Yellowstone National Park.	Kyndt JA, Robertson S, Shoffstall IB, Ramaley RF, Meyer TE.	Microorganisms	10.3390/microorganisms10061169	2022
	Dictyostelium transcriptional host cell response upon infection with Legionella.	Farbrother P, Wagner C, Na J, Tunggal B, Morio T, Urushihara H, Tanaka Y, Schleicher M, Steinert M, Eichinger L.	Cell Microbiol	10.1111/j.1462-5822.2005.00633.x	2006
	The Legionella collagen-like protein employs a distinct binding mechanism for the recognition of host glycosaminoglycans.	Rehman S, Antonovic AK, McIntire IE, Zheng H, Cleaver L, Baczynska M, Adams CO, Portlock T, Richardson K, Shaw R, Oregioni A, Mastroianni G, Whittaker SB, Kelly G, Lorenz CD, Fornili A, Cianciotto NP, Garnett JA.	Nat Commun	10.1038/s41467-024-49255-4	2024
	Legionella in two splenectomized patients. Coincidence or causal relationship?	Gorelik O, Lazarovich Z, Boldur I, Almoznino-Sarafian D, Alon I, Modai D, Cohen N.	Infection	10.1007/s15010-004-3005-4	2004
Genetics	Host Adaptation in Legionellales Is 1.9 Ga, Coincident with Eukaryogenesis.	Hugoson E, Guliaev A, Ammunet T, Guy L.	Mol Biol Evol	10.1093/molbev/msac037	2022
Phylogeny	Relapsing life threatening community acquired pneumonia due to rare Legionella species responsive to ceftriaxone and aztreonam.	Zamar Akhtar U, Yousef S, Khalid al-M, Adolphus OS.	Afr J Med Med Sci		1998
	Chemical characterization of lipopolysaccharides from Legionella feeleii, Legionella hackeliae and Legionella jordanis.	Sonesson A, Jantzen E, Tangen T, Zahringer U.	Microbiology (Reading)	10.1099/00221287-140-10-2663	1994
	Structure, Dynamics and Cellular Insight Into Novel Substrates of the Legionella pneumophila Type II Secretion System.	Portlock TJ, Tyson JY, Dantu SC, Rehman S, White RC, McIntire IE, Sewell L, Richardson K, Shaw R, Pandini A, Cianciotto NP, Garnett JA.	Front Mol Biosci	10.3389/fmolb.2020.00112	2020
	Immunologic characterization and specificity of three monoclonal antibodies against the 58-kilodalton protein of Legionella pneumophila.	Sampson JS, Plikaytis BB, Aloisio CH, Carlone GM, Pau CP, Stinson AR.	J Clin Microbiol	10.1128/jcm.29.4.836-841.1991	1991
	Active and adaptive Legionella CRISPR-Cas reveals a recurrent challenge to the pathogen.	Rao C, Guyard C, Pelaz C, Wasserscheid J, Bondy-Denomy J, Dewar K, Ensminger AW.	Cell Microbiol	10.1111/cmi.12586	2016
	[Sources and fate of pathogenic microorganisms in aquatic environments].	Baudart J, Paniel N.	Rev Francoph Lab	10.1016/s1773-035x(14)72362-7	2014
	Coevolution between nonhomologous but functionally similar proteins and their conserved partners in the Legionella pathogenesis system.	Feldman M, Zusman T, Hagag S, Segal G.	Proc Natl Acad Sci U S A	10.1073/pnas.0501850102	2005
	Characterization of the alternative sigma factor sigma54 and the transcriptional regulator FleQ of Legionella pneumophila, which are both involved in the regulation cascade of flagellar gene expression.	Jacobi S, Schade R, Heuner K.	J Bacteriol	10.1128/jb.186.9.2540-2547.2004	2004
Genetics	Comparative analyses of Legionella species identifies genetic features of strains causing Legionnaires' disease.	Gomez-Valero L, Rusniok C, Rolando M, Neou M, Dervins-Ravault D, Demirtas J, Rouy Z, Moore RJ, Chen H, Petty NK, Jarraud S, Etienne J, Steinert M, Heuner K, Gribaldo S, Medigue C, Glockner G, Hartland EL, Buchrieser C.	Genome Biol	10.1186/preaccept-1086350395137407	2014
Enzymology	Rapid method for enumeration of viable Legionella pneumophila and other Legionella spp. in water.	Delgado-Viscogliosi P, Simonart T, Parent V, Marchand G, Dobbelaere M, Pierlot E, Pierzo V, Menard-Szczebara F, Gaudard-Ferveur E, Delabre K, Delattre JM.	Appl Environ Microbiol	10.1128/aem.71.7.4086-4096.2005	2005
	Compilation of small ribosomal subunit RNA structures.	Neefs JM, Van de Peer Y, De Rijk P, Chapelle S, De Wachter R.	Nucleic Acids Res	10.1093/nar/21.13.3025	1993
Phylogeny	Second serogroup of Legionella hackeliae isolated from a patient with pneumonia.	Wilkinson HW, Thacker WL, Steigerwalt AG, Brenner DJ, Ampel NM, Wing EJ	J Clin Microbiol	10.1128/jcm.22.4.488-489.1985	1985
Phylogeny	Legionella indianapolisensis sp. nov., isolated from a patient with pulmonary abscess.	Relich RF, Schmitt BH, Raposo H, Barker L, Blosser SJ, May M.	Int J Infect Dis	10.1016/j.ijid.2018.01.024	2018

References

#7972	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19214
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#40810	; Curators of the CIP;
#50487	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 31232 A
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#119759	Collection of Institut Pasteur ; Curators of the CIP; CIP 103844
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Legionella hackeliae (DSM 19214, ATCC 35250, CIP 103844)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Metabolite production

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000953655

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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