Legionella wadsworthii (DSM 21896, ATCC 33877, CCUG 16415)

Name: Legionella wadsworthii (DSM 21896, ATCC 33877, CCUG 16415)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6746

Type strain

Strain Designation Edelstein Wadsworth 81-716A, 81-716A

Culture col. no. DSM 21896 ATCC 33877 CCUG 16415 CIP 103886 Edelstein Wadsworth 81-716A 5 more

NCBI tax ID(s) 1122170 28088

Special Collections DSMZ CCUG CIP

History <- CCUG; CCUG 16415 <- CDC, Atlanta; 81-716A CIP <- 2000, F. Grimont, Inst. Pasteur, Paris, France <- CNR Legionella, Lyon, France <- P.H. Edelstein: strain Wadsworth 81-716A

Links

Legionella wadsworthii Edelstein Wadsworth 81-716A is a microaerophile, Gram-negative, rod-shaped bacterium that was isolated from Human sputum; patient with pneumonia.

Gram-negative rod-shaped microaerophile genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 40739

DSM 21896,ATCC 33877,NCTC 11532,CCUG 16415,CIP 103886,CCUG 58308

@ref 20215

Last LPSN update 2026-02-09 11:19:18

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Legionellales

Family Legionellaceae

Genus Legionella

Species Legionella wadsworthii

Full scientific name Legionella wadsworthii Edelstein et al. 1983

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
123901	negative	rod-shaped
125438	negative		97.9
125439	negative		99.3

Colony morphology

16025

Incubation period1-2 days

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
16025	BCYE-AGAR (DSMZ Medium 585)	Medium recipe at MediaDive	Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
34430	MEDIUM 23 - for Afipia and Legionella		Distilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
123901	CIP Medium 23	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
16025	positive	growth	37
34430	positive	growth	37
123901	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
16025	microaerophile
125439	obligate aerobe	99.7

Spore formation

@ref	Spore formation	Confidence
125438		91.156
125439		97.6

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
123901	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
123901	oxidase	+
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
123901	-	+	+	+	-	+	+	-	-	-	+	+	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Infection	#Disease	-
#Infection	#Patient	-
#Host	#Human	-
#Host Body Product	#Fluids	#Sputum

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude
16025	Human sputum; patient with pneumonia	Los Angeles, California	USA	USA	North America	34.0522	-118.244 34.0522/-118.244
123901	Human, Sputum

Taxonmaps

69479

Global distribution of 16S sequence Z49738 (>99% sequence identity) for Legionella from Microbeatlas

Aquatic Samples	33
Soil Samples	10
Animal Samples	1
Plant Samples
Total Samples	44

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
16025	2	Risk group (German classification) According to TRBA 466
123901	2	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	42650_E02 assembly for Legionella wadsworthii NCTC11532	contig	GCA_900452925	28088.5	8031876210	28088	78.77
66792	ASM70126v1 assembly for Legionella wadsworthii DSM 21896 = ATCC 33877	scaffold	GCA_000701265	1122170.4	2561511070	1122170	73.97

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	L.wadsworthii gene for ribosomal RNA, small subunit	Z49738	1472	28088
16025	L.wadsworthii (ATCC 33877) gene for 16S rRNA	X73401	1467	28088
124043	Legionella wadsworthii strain MDC1602 16S ribosomal RNA gene, partial sequence.	JF720382	524	28088

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Legionella wadsworthii DSM 21896 = ATCC 33877
NCBI: GCA_000701265

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.70	no
125439	gram_stain	BacteriaNetⓘ	negative	99.30	no
125439	motility	BacteriaNetⓘ	no	60.80	no
125439	spore_formation	BacteriaNetⓘ	no	97.60	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Legionella wadsworthii DSM 21896 = ATCC 33877 ATCC 33877
NCBI: GCA_000701265.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	97.90	no
125438	anaerobic	anaerobicⓘ	no	96.70	no
125438	aerobic	aerobicⓘ	no	73.17	yes
125438	spore-forming	spore-formingⓘ	no	91.16	no
125438	thermophilic	thermophileⓘ	no	96.49	yes
125438	flagellated	motile2+ⓘ	no	59.17	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Rapid Detection and Differentiation of Legionella pneumophila and Non-Legionella pneumophila Species by Using Recombinase Polymerase Amplification Combined With EuNPs-Based Lateral Flow Immunochromatography.	Du J, Ma B, Li J, Wang Y, Dou T, Xu S, Zhang M.	Front Chem	10.3389/fchem.2021.815189	2021
	Rapid PCR Detection of Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum.	Cunningham SA, Mandrekar JN, Rosenblatt JE, Patel R.	Int J Bacteriol	10.1155/2013/168742	2013
Phylogeny	Legionella confirmation using real-time PCR and SYTO9 is an alternative to current methodology.	Giglio S, Monis PT, Saint CP.	Appl Environ Microbiol	10.1128/aem.71.12.8944-8948.2005	2005
Enzymology	Development and evaluation of Chlamylege, a new commercial test allowing simultaneous detection and identification of Legionella, Chlamydophila pneumoniae, and Mycoplasma pneumoniae in clinical respiratory specimens by multiplex PCR.	Ginevra C, Barranger C, Ros A, Mory O, Stephan JL, Freymuth F, Joannes M, Pozzetto B, Grattard F.	J Clin Microbiol	10.1128/jcm.43.7.3247-3254.2005	2005
Phylogeny	High diversity and abundance of Legionella spp. in a pristine river and impact of seasonal and anthropogenic effects.	Parthuisot N, West NJ, Lebaron P, Baudart J.	Appl Environ Microbiol	10.1128/aem.00188-10	2010
Metabolism	Targeting species-specific low-affinity 16S rRNA binding sites by using peptide nucleic acids for detection of Legionellae in biofilms.	Wilks SA, Keevil CW.	Appl Environ Microbiol	10.1128/aem.02918-05	2006
Phylogeny	Clinical laboratory differentiation of Legionellaceae family members with pigment production and fluorescence on media supplemented with aromatic substrates.	Vickers RM, Yu VL.	J Clin Microbiol	10.1128/jcm.19.5.583-587.1984	1984
Metabolism	Photoreactivation of UV-irradiated Legionella pneumophila and other Legionella species.	Knudson GB.	Appl Environ Microbiol	10.1128/aem.49.4.975-980.1985	1985
Biotechnology	Detection of Salmonella spp. in oysters by PCR.	Bej AK, Mahbubani MH, Boyce MJ, Atlas RM.	Appl Environ Microbiol	10.1128/aem.60.1.368-373.1994	1994
	DNA probe specific for Legionella pneumophila.	Grimont PA, Grimont F, Desplaces N, Tchen P.	J Clin Microbiol	10.1128/jcm.21.3.431-437.1985	1985
Enzymology	Legionella sainthelensi: a new species of Legionella isolated from water near Mt. St. Helens.	Campbell J, Bibb WF, Lambert MA, Eng S, Steigerwalt AG, Allard J, Moss CW, Brenner DJ.	Appl Environ Microbiol	10.1128/aem.47.2.369-373.1984	1984
	Genetic structure of populations of Legionella pneumophila.	Selander RK, McKinney RM, Whittam TS, Bibb WF, Brenner DJ, Nolte FS, Pattison PE.	J Bacteriol	10.1128/jb.163.3.1021-1037.1985	1985
Metabolism	Non-canonical activation of the ER stress sensor ATF6 by Legionella pneumophila effectors.	Ibe NU, Subramanian A, Mukherjee S.	Life Sci Alliance	10.26508/lsa.202101247	2021
Genetics	Genomic characterization and assessment of pathogenic potential of Legionella spp. isolates from environmental monitoring.	Svetlicic E, Jaen-Luchoro D, Klobucar RS, Jers C, Kazazic S, Franjevic D, Klobucar G, Shelton BG, Mijakovic I.	Front Microbiol	10.3389/fmicb.2022.1091964	2022
Phylogeny	Rapid detection and typing of pathogenic nonpneumophila Legionella spp. isolates using a multiplex real-time PCR assay.	Benitez AJ, Winchell JM.	Diagn Microbiol Infect Dis	10.1016/j.diagmicrobio.2016.01.007	2016
Enzymology	Presence and Persistence of Viable, Clinically Relevant Legionella pneumophila Bacteria in Garden Soil in the Netherlands.	van Heijnsbergen E, van Deursen A, Bouwknegt M, Bruin JP, de Roda Husman AM, Schalk JA.	Appl Environ Microbiol	10.1128/aem.00595-16	2016
Pathogenicity	Role of 16S rRNA Helix 44 in Ribosomal Resistance to Hygromycin B.	Pfister P, Risch M, Brodersen DE, Bottger EC.	Antimicrob Agents Chemother	10.1128/aac.47.5.1496-1502.2003	2003
Genetics	Comparative analyses of Legionella species identifies genetic features of strains causing Legionnaires' disease.	Gomez-Valero L, Rusniok C, Rolando M, Neou M, Dervins-Ravault D, Demirtas J, Rouy Z, Moore RJ, Chen H, Petty NK, Jarraud S, Etienne J, Steinert M, Heuner K, Gribaldo S, Medigue C, Glockner G, Hartland EL, Buchrieser C.	Genome Biol	10.1186/preaccept-1086350395137407	2014
Enzymology	Determination of catalase, peroxidase, and superoxide dismutase within the genus Legionella.	Pine L, Hoffman PS, Malcolm GB, Benson RF, Keen MG.	J Clin Microbiol	10.1128/jcm.20.3.421-429.1984	1984
Phylogeny	Molecular characterization of Legionella populations present within slow sand filters used for fungal plant pathogen suppression in horticultural crops.	Calvo-Bado LA, Morgan JA, Sergeant M, Pettitt TR, Whipps JM.	Appl Environ Microbiol	10.1128/aem.69.1.533-541.2003	2003
Pathogenicity	Legionella wadsworthii species nova: a cause of human pneumonia.	Edelstein PH, Brenner DJ, Moss CW, Steigerwalt AG, Francis EM, George WL	Ann Intern Med	10.7326/0003-4819-97-6-809	1982
Phylogeny	Characterization of a Novel Species of Legionella Isolated from a Healthcare Facility: Legionella resiliens sp. nov.	Cristino S, Pascale MR, Marino F, Derelitto C, Salaris S, Orsini M, Squarzoni S, Grottola A, Girolamini L.	Pathogens	10.3390/pathogens13030250	2024

References

#16025	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21896
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#34430	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#123901	Collection of Institut Pasteur ; Curators of the CIP; CIP 103886
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Legionella wadsworthii (DSM 21896, ATCC 33877, CCUG 16415)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_900452925

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

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