Legionella sainthelensi (DSM 19231, ATCC 35248, CCUG 29672)

Name: Legionella sainthelensi (DSM 19231, ATCC 35248, CCUG 29672)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6744

Type strain

Strain Designation MSH-4, Mt. St. Helens 4, strain 4

Culture col. no. DSM 19231 ATCC 35248 CCUG 29672 CIP 103885 NCTC 11988 3 more

NCBI tax ID(s) 1408445 28087

Special Collections DSMZ CCUG CIP

History DSMZ <- H. E. Müller <- ATCC <- W. F. Bibb; MSH-4 <- J. Campbell; strain 4 CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- W.F. Bibb: strain MSH-4 <- J. Campbell: strain 4

Links

Legionella sainthelensi MSH-4 is a microaerophile, Gram-negative, rod-shaped bacterium that was isolated from spring water.

Gram-negative rod-shaped microaerophile genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 40945

ATCC 35248,NCTC 11988,CCUG 29672,CIP 103885,DSM 19231

@ref 20215

Last LPSN update 2026-05-29 08:26:29

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Legionellales

Family Legionellaceae

Genus Legionella

Species Legionella sainthelensi

Full scientific name Legionella sainthelensi Campbell et al. 1984

BacDive ID	Other strains from **Legionella sainthelensi** (1)	Type strain
6745	L. sainthelensi 1489-CA-H, DSM 25322, ATCC 49322, CIP 105115, ...

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
122351	negative	rod-shaped
125438	negative		98.985
125439	negative		94.833

Colony morphology

7980

Incubation period3-7 days

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
7980	BCYE-AGAR (DSMZ Medium 585)	Medium recipe at MediaDive	Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
34085	MEDIUM 23 - for Afipia and Legionella		Distilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
122351	CIP Medium 23	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
7980	positive	growth	37
34085	positive	growth	37
49708	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
7980	microaerophile
49708	microaerophile
125439	obligate aerobe	98.204

Spore formation

@ref	Spore formation	Confidence
125439		98.921

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
7980	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
7980	cytochrome-c oxidase	-	1.9.3.1
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	-		from API zym
68382	leucine arylamidase	-	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
122351	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Spring

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Sampling date
7980	spring water	Mt. St. Helens	USA	USA	North America
49708	Stream water					1981
122351	Environment, Spring water	Mount Saint Helens, Washington	United States of America	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence Z49734 (>99% sequence identity) for Legionella from Microbeatlas

Aquatic Samples	1090
Soil Samples	142
Animal Samples	151
Plant Samples	31
Total Samples	1414

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
7980	2	Risk group (German classification) According to TRBA 466
122351	2	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	52451_A01 assembly for Legionella sainthelensi NCTC11988	complete	GCA_900637685	28087.10	2848179527	28087	93.39
66792	ASM62168v1 assembly for Legionella sainthelensi ATCC 35248 = DSM 19231	scaffold	GCA_000621685	1408445.3	2558309002	1408445	64.67
66792	Leg_sainthelensi_MSH-4.v1 assembly for Legionella sainthelensi MSH-4	contig	GCA_900640185	28087.11	8115343376	28087	62.08
124043	ASM146810v1 assembly for Legionella sainthelensi Mt.St.Helens-4	scaffold	GCA_001468105	28087.4	2663762905	28087	58.82

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Legionella sainthelensi strain MDC1588 16S ribosomal RNA gene, partial sequence	JF720399	524	28087
20218	L.sainthelensi gene for ribosomal RNA, small subunit	Z49734	1405	28087
7980	L.sainthelensi (ATCC 35248) gene for 16S rRNA	X73399	1467	28087

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: 52451_A01 assembly for Legionella sainthelensi NCTC11988
NCBI: GCA_900637685

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.20	no
125439	gram_stain	BacteriaNetⓘ	negative	94.83	no
125439	motility	BacteriaNetⓘ	yes	43.37	no
125439	spore_formation	BacteriaNetⓘ	no	98.92	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Legionella sainthelensi NCTC11988
NCBI: GCA_900637685.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	98.99	no
125438	anaerobic	anaerobicⓘ	no	97.95	yes
125438	aerobic	aerobicⓘ	no	75.72	yes
125438	spore-forming	spore-formingⓘ	no	89.53	no
125438	thermophilic	thermophileⓘ	no	96.94	yes
125438	flagellated	motile2+ⓘ	no	55.53	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Complete Genome Sequence of Legionella sainthelensi Isolated from a Patient with Legionnaires' Disease.	Slow S, Anderson T, Biggs P, Kennedy M, Murdoch D, Cree S.	Genome Announc	10.1128/genomea.01588-17	2018
Phylogeny	Development of quantitative PCR assays targeting the 16S rRNA genes of Enterococcus spp. and their application to the identification of enterococcus species in environmental samples.	Ryu H, Henson M, Elk M, Toledo-Hernandez C, Griffith J, Blackwood D, Noble R, Gourmelon M, Glassmeyer S, Santo Domingo JW.	Appl Environ Microbiol	10.1128/aem.02802-12	2013
Phylogeny	Legionella confirmation using real-time PCR and SYTO9 is an alternative to current methodology.	Giglio S, Monis PT, Saint CP.	Appl Environ Microbiol	10.1128/aem.71.12.8944-8948.2005	2005
Enzymology	Development and evaluation of Chlamylege, a new commercial test allowing simultaneous detection and identification of Legionella, Chlamydophila pneumoniae, and Mycoplasma pneumoniae in clinical respiratory specimens by multiplex PCR.	Ginevra C, Barranger C, Ros A, Mory O, Stephan JL, Freymuth F, Joannes M, Pozzetto B, Grattard F.	J Clin Microbiol	10.1128/jcm.43.7.3247-3254.2005	2005
Phylogeny	Isolation of Legionella oakridgensis from two patients with pleural effusion living in the same geographical area.	Lo Presti F, Riffard S, Jarraud S, Le Gallou F, Richet H, Vandenesch F, Etienne J.	J Clin Microbiol	10.1128/jcm.38.8.3128-3130.2000	2000
Biotechnology	Detection of Salmonella spp. in oysters by PCR.	Bej AK, Mahbubani MH, Boyce MJ, Atlas RM.	Appl Environ Microbiol	10.1128/aem.60.1.368-373.1994	1994
Genetics	NCTC3000: a century of bacterial strain collecting leads to a rich genomic data resource.	Dicks J, Fazal MA, Oliver K, Grayson NE, Turnbull JD, Bane E, Burnett E, Deheer-Graham A, Holroyd N, Kaushal D, Keane J, Langridge G, Lomax J, McGregor H, Picton S, Quail M, Singh D, Tracey A, Korlach J, Russell JE, Alexander S, Parkhill J.	Microb Genom	10.1099/mgen.0.000976	2023
Phylogeny	Legionella sainthelensi: a new species of Legionella isolated from water near Mt. St. Helens.	Campbell J, Bibb WF, Lambert MA, Eng S, Steigerwalt AG, Allard J, Moss CW, Brenner DJ	Appl Environ Microbiol	10.1128/aem.47.2.369-373.1984	1984

References

#7980	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19231
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#34085	; Curators of the CIP;
#49708	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 29672
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#122351	Collection of Institut Pasteur ; Curators of the CIP; CIP 103885
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Legionella sainthelensi (DSM 19231, ATCC 35248, CCUG 29672)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_900637685

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media