Legionella gormanii (DSM 16641, ATCC 33297, CCUG 12267)

Name: Legionella gormanii (DSM 16641, ATCC 33297, CCUG 12267)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6708

Type strain

Strain Designation LS-13

Variant Isotype of BacDive ID 6709

Culture col. no. DSM 16641 ATCC 33297 CCUG 12267 CIP 104724 NCTC 11401 1 more

NCBI tax ID(s) 464

Special Collections DSMZ CCUG CIP

History <- ATCC; ATCC 33297 <- G. K. Morris; <- G. W. Gorman; LS-13 CIP <- 1996, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- G.K. Morris: strain LS-13 <- G. Gorman

Links

Legionella gormanii LS-13 is a microaerophile, Gram-negative, rod-shaped bacterium that was isolated from soil from a creek bank.

Gram-negative rod-shaped microaerophile genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 40452

ATCC 33342,ATCC 33297,CCUG 12267,NCTC 11401,CIP 104724,DSM 16641

@ref 20215

Last LPSN update 2026-02-09 11:19:17

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Legionellales

Family Legionellaceae

Genus Legionella

Species Legionella gormanii

Full scientific name Legionella gormanii Morris et al. 1980

Synonyms (1)

Fluoribacter gormanii

BacDive ID	Other strains from **Legionella gormanii** (2)	Type strain
6709	L. gormanii LS-13 (ALLO3), DSM 25296
158045	L. gormanii R55, DSM 109130

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
119712	negative	rod-shaped
125439	negative		99.8
125438	negative		99.494

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
6544	BCYE-AGAR (DSMZ Medium 585)	Medium recipe at MediaDive	Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
38670	MEDIUM 23 - for Afipia and Legionella		Distilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
119712	CIP Medium 23	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
6544	positive	growth	37
38670	positive	growth	37
119712	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
6544	microaerophile
125439	obligate aerobe	98.7

Spore formation

@ref	Spore formation	Confidence
125439		99.7
125438		91.067

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
119712	17632 ChEBI	nitrate	-	reduction
119712	16301 ChEBI	nitrite	-	reduction

Metabolite production

@ref	Chebi-ID	Metabolite	Production
119712	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
6544	catalase	+	1.11.1.6
119712	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
6544	cytochrome-c oxidase	-	1.9.3.1
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
119712	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
119712	urease	-	3.5.1.5
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
119712	-	+	+	+	-	+	+	-	-	-	+	+	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#River (Creek)
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
6544	soil from a creek bank	Atlanta, Georgia	USA	USA	North America
119712	Environment, Soil from a creek bank	Atlanta, Georgia	United States of America	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence Z32639 (>99% sequence identity) for Legionella from Microbeatlas

Aquatic Samples	9
Soil Samples	2
Animal Samples
Plant Samples	1
Total Samples	12

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6544	2	Risk group (German classification) According to TRBA 466
119712	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	48903_B01 assembly for Fluoribacter gormanii NCTC11401	contig	GCA_900450965	464.13	2839705416	464	78.97
66792	ASM164868v1 assembly for Fluoribacter gormanii ATCC 33297	scaffold	GCA_001648685	464.6		464	65.7
124043	ASM146768v1 assembly for Fluoribacter gormanii LS-13	scaffold	GCA_001467685	464.5	2698536327	464	59.56

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Fluoribacter gormanii strain MDC1609 16S ribosomal RNA gene, partial sequence	JF720383	522		464
6544	L.gormanii (LS-13) gene for 16S ribosomal RNA	Z32639	1450		464

GC content

6544

GC-content (mol%)40.7

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Fluoribacter gormanii ATCC 33297
NCBI: GCA_001648685

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	99.70	no
125439	motility	BacteriaNetⓘ	yes	61.30	no
125439	gram_stain	BacteriaNetⓘ	negative	99.80	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.70	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Fluoribacter gormanii ATCC 33297
NCBI: GCA_001648685.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	99.49	no
125438	anaerobic	anaerobicⓘ	no	96.23	yes
125438	aerobic	aerobicⓘ	no	78.59	yes
125438	spore-forming	spore-formingⓘ	no	91.07	no
125438	thermophilic	thermophileⓘ	no	96.50	yes
125438	flagellated	motile2+ⓘ	yes	66.85	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Thermodynamic Study on Biomimetic Legionella gormanii Bacterial Membranes.	Pastuszak K, Palusinska-Szysz M, Wiacek AE, Jurak M.	Molecules	10.3390/molecules29184367	2024
	Unusual Lipid Components of Legionella gormanii Membranes.	Chmiel E, Galuska CE, Koper P, Kowalczyk B, Urbanik-Sypniewska T, Palusinska-Szysz M, Fuchs B.	Metabolites	10.3390/metabo12050418	2022
	Physicochemical Characteristics of Model Membranes Composed of Legionella gormanii Lipids.	Pastuszak K, Chmiel E, Kowalczyk B, Tarasiuk J, Jurak M, Palusinska-Szysz M.	Membranes (Basel)	10.3390/membranes13030356	2023
	Insight into the Mechanism of Interactions between the LL-37 Peptide and Model Membranes of Legionella gormanii Bacteria.	Pastuszak K, Kowalczyk B, Tarasiuk J, Luchowski R, Gruszecki WI, Jurak M, Palusinska-Szysz M.	Int J Mol Sci	10.3390/ijms241512039	2023
Phylogeny	New Insight regarding Legionella Non-Pneumophila Species Identification: Comparison between the Traditional mip Gene Classification Scheme and a Newly Proposed Scheme Targeting the rpoB Gene.	Pascale MR, Salaris S, Mazzotta M, Girolamini L, Fregni Serpini G, Manni L, Grottola A, Cristino S.	Microbiol Spectr	10.1128/spectrum.01161-21	2021
Enzymology	Biochemical properties of beta-lactamase produced by Legionella gormanii.	Fujii T, Sato K, Miyata K, Inoue M, Mitsuhashi S.	Antimicrob Agents Chemother	10.1128/aac.29.5.925	1986
Phylogeny	Legionella confirmation using real-time PCR and SYTO9 is an alternative to current methodology.	Giglio S, Monis PT, Saint CP.	Appl Environ Microbiol	10.1128/aem.71.12.8944-8948.2005	2005
Enzymology	Biochemical characterization of the THIN-B metallo-beta-lactamase of Janthinobacterium lividum.	Docquier JD, Lopizzo T, Liberatori S, Prenna M, Thaller MC, Frere JM, Rossolini GM.	Antimicrob Agents Chemother	10.1128/aac.48.12.4778-4783.2004	2004
Enzymology	Rapid method for enumeration of viable Legionella pneumophila and other Legionella spp. in water.	Delgado-Viscogliosi P, Simonart T, Parent V, Marchand G, Dobbelaere M, Pierlot E, Pierzo V, Menard-Szczebara F, Gaudard-Ferveur E, Delabre K, Delattre JM.	Appl Environ Microbiol	10.1128/aem.71.7.4086-4096.2005	2005
Enzymology	Development and evaluation of Chlamylege, a new commercial test allowing simultaneous detection and identification of Legionella, Chlamydophila pneumoniae, and Mycoplasma pneumoniae in clinical respiratory specimens by multiplex PCR.	Ginevra C, Barranger C, Ros A, Mory O, Stephan JL, Freymuth F, Joannes M, Pozzetto B, Grattard F.	J Clin Microbiol	10.1128/jcm.43.7.3247-3254.2005	2005
Metabolism	Lgt: a family of cytotoxic glucosyltransferases produced by Legionella pneumophila.	Belyi Y, Tabakova I, Stahl M, Aktories K.	J Bacteriol	10.1128/jb.01798-07	2008
	Characterization of the alternative sigma factor sigma54 and the transcriptional regulator FleQ of Legionella pneumophila, which are both involved in the regulation cascade of flagellar gene expression.	Jacobi S, Schade R, Heuner K.	J Bacteriol	10.1128/jb.186.9.2540-2547.2004	2004
Phylogeny	Legionella bozemanii serogroup 2: a new etiological agent.	Tang PW, Toma S, Moss CW, Steigerwalt AG, Cooligan TG, Brenner DJ.	J Clin Microbiol	10.1128/jcm.19.1.30-33.1984	1984
	DNA probe specific for Legionella pneumophila.	Grimont PA, Grimont F, Desplaces N, Tchen P.	J Clin Microbiol	10.1128/jcm.21.3.431-437.1985	1985
Enzymology	Legionella sainthelensi: a new species of Legionella isolated from water near Mt. St. Helens.	Campbell J, Bibb WF, Lambert MA, Eng S, Steigerwalt AG, Allard J, Moss CW, Brenner DJ.	Appl Environ Microbiol	10.1128/aem.47.2.369-373.1984	1984
Phylogeny	Serological and genotypic diversity among serogroup 5- reacting environmental Legionella isolates.	Garrity GM, Elder EM, Davis B, Vickers RM, Brown A.	J Clin Microbiol	10.1128/jcm.15.4.646-653.1982	1982
	Genus-specific epitope on the 60-kilodalton Legionella heat shock protein recognized by a monoclonal antibody.	Steinmetz I, Rheinheimer C, Hubner I, Bitter-Suermann D.	J Clin Microbiol	10.1128/jcm.29.2.346-354.1991	1991
	Cross-reactions between Legionella pneumophila (serogroup 1) and twenty-eight other bacterial species, including other members of the family Legionellaceae.	Collins MT, Espersen F, Hoiby N, Cho SN, Friis-Moller A, Reif JS.	Infect Immun	10.1128/iai.39.3.1441-1456.1983	1983
	Distribution of Secretion Systems in the Genus Legionella and Its Correlation with Pathogenicity.	Qin T, Zhou H, Ren H, Liu W	Front Microbiol	10.3389/fmicb.2017.00388	2017
Genetics	Sequence and genome context analysis of a new molecular class D beta-lactamase gene from Legionella pneumophila.	Avison MB, Simm AM	J Antimicrob Chemother	10.1093/jac/dkf135	2002
Enzymology	Characterization of OXA-29 from Legionella (Fluoribacter) gormanii: molecular class D beta-lactamase with unusual properties.	Franceschini N, Boschi L, Pollini S, Herman R, Perilli M, Galleni M, Frere JM, Amicosante G, Rossolini GM	Antimicrob Agents Chemother	10.1128/AAC.45.12.3509-3516.2001	2001
Enzymology	Biochemical characterization of the FEZ-1 metallo-beta-lactamase of Legionella gormanii ATCC 33297T produced in Escherichia coli.	Mercuri PS, Bouillenne F, Boschi L, Lamotte-Brasseur J, Amicosante G, Devreese B, van Beeumen J, Frere JM, Rossolini GM, Galleni M	Antimicrob Agents Chemother	10.1128/AAC.45.4.1254-1262.2001	2001
Enzymology	The Legionella (Fluoribacter) gormanii metallo-beta-lactamase: a new member of the highly divergent lineage of molecular-subclass B3 beta-lactamases.	Boschi L, Mercuri PS, Riccio ML, Amicosante G, Galleni M, Frere JM, Rossolini GM	Antimicrob Agents Chemother	10.1128/AAC.44.6.1538-1543.2000	2000
Enzymology	Legionella gormanii sp. nov.	Morris GK, Steigerwalt A, Feeley JC, Wong ES, Martin WT, Patton CM, Brenner DJ.	J Clin Microbiol	10.1128/jcm.12.5.718-721.1980	1980

References

#6544	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16641
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#38670	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#119712	Collection of Institut Pasteur ; Curators of the CIP; CIP 104724
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Legionella gormanii (DSM 16641, ATCC 33297, CCUG 12267)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Metabolite production

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_900450965

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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