Name: Legionella gormanii LS-13
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6708

Type strain:

Species: Legionella gormanii

Strain Designation: LS-13

Variant: Isotype of BacDive ID 6709

Culture col. no.: DSM 16641, ATCC 33297, CCUG 12267, CIP 104724, NCTC 11401

Strain history: CIP <- 1996, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- G.K. Morris: strain LS-13 <- G. Gorman

NCBI tax ID(s): 464 (species)

SI-ID 40452

ATCC 33342, ATCC 33297, CCUG 12267, NCTC 11401, CIP 104724, DSM 16641

Other strains from Legionella gormanii

L. gormanii LS-13 (ALLO3), DSM 25296
L. gormanii R55, DSM 109130

Section

Legionella gormanii LS-13 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from soil from a creek bank.

Gram-negative
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Legionellales*
	Family	*Legionellaceae*
	Genus	*Legionella*
	Species	**Legionella gormanii**
	Full Scientific Name (LPSN)	Legionella gormanii Morris et al. 1980

Synonym

Fluoribacter gormanii

Information on morphological and physiological properties Morphology

[Ref.: #119712]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.995

[Ref.: #119712]	Cell shape	rod-shaped

[Ref.: #119712]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6544]

Culture medium

BCYE-AGAR (DSMZ Medium 585)

[Ref.: #6544]

Culture medium growth

[Ref.: #6544]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6544]

Culture medium composition

expand / minimize

[Ref.: #38670]

Culture medium

MEDIUM 23 - for Afipia and Legionella

[Ref.: #38670]

Culture medium growth

[Ref.: #38670]

Culture medium composition

expand / minimize

[Ref.: #119712]

Culture medium

CIP Medium 23

[Ref.: #119712]

Culture medium growth

[Ref.: #119712]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #6544]	growth	37 °C
[Ref.: #38670]	growth	37 °C
[Ref.: #119712]	growth	37 °C

[Ref.: #6544]	Temperature range	mesophilic
[Ref.: #38670]	Temperature range	mesophilic
[Ref.: #119712]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #6544]

Oxygen tolerance

microaerophile

[Ref.: #69481]	Ability of spore formation	no Confidence in %99
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.949

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #119712]		nitrate ChEBI	-	reduction
[Ref.: #119712]		nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #119712]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #6544]	catalase	+	1.11.1.6
[Ref.: #119712]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #6544]	cytochrome-c oxidase	-	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #119712]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #119712]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #119712]	8152	-	+	+	+	-	+	+	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6544]	Sample type/isolated from	soil from a creek bank
[Ref.: #6544]	Geographic location (country and/or sea, region)	Atlanta, Georgia
[Ref.: #6544]	Country	USA
[Ref.: #6544]	Country ISO 3 Code	USA
[Ref.: #6544]	Continent	North America

[Ref.: #119712]	Sample type/isolated from	Environment, Soil from a creek bank
[Ref.: #119712]	Geographic location (country and/or sea, region)	Atlanta, Georgia
[Ref.: #119712]	Country	United States of America
[Ref.: #119712]	Country ISO 3 Code	USA
[Ref.: #119712]	Continent	North America
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#River (Creek)
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence Z32639 (>99% sequence identity) for Legionella from Microbeatlas

Aquatic Samples	9
Soil Samples	2
Animal Samples
Plant Samples	1
Total Samples	12

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6544]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #119712]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6544]	L.gormanii (LS-13) gene for 16S ribosomal RNA	Z32639	1450	ENA	464 tax ID
[Ref.: #20218]	Fluoribacter gormanii strain MDC1609 16S ribosomal RNA gene, partial sequence	JF720383	522	ENA	464 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Fluoribacter gormanii NCTC11401	GCA_900450965	contig	GenBank	464 tax ID	*
[Ref.: #66792]	Fluoribacter gormanii ATCC 33297	GCA_001648685	scaffold	GenBank	464 tax ID	*
[Ref.: #66792]	Fluoribacter gormanii strain ATCC 33297	464.6	wgs	PATRIC	464 tax ID	*
[Ref.: #66792]	Fluoribacter gormanii strain LS-13	464.5	wgs	PATRIC	464 tax ID	*
[Ref.: #66792]	Fluoribacter gormanii strain NCTC11401	464.13	wgs	PATRIC	464 tax ID	*
[Ref.: #66792]	Fluoribacter gormanii NCTC 11401	2839705416	draft	JGI IMG/M	464 tax ID	*

[Ref.: #6544]

GC-content

40.7 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Fluoribacter gormanii strain NCTC11401 PATRIC: 464.13
[Ref.: #69481]	spore-forming	no	99	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Fluoribacter gormanii ATCC 33297 NCBI: GCA_001648685.1
[Ref.: #69480]	flagellated	no	71.392	no
[Ref.: #69480]	gram-positive	no	98.528	no
[Ref.: #69480]	anaerobic	no	98.625	yes
[Ref.: #69480]	halophile	no	89.078	no
[Ref.: #69480]	spore-forming	no	93.259	no
[Ref.: #69480]	glucose-util	yes	64.506	no
[Ref.: #69480]	motile	yes	88.239	no
[Ref.: #69480]	aerobic	no	91.587	no
[Ref.: #69480]	thermophile	no	99.045	yes
[Ref.: #69480]	glucose-ferment	no	87.319	no

Availability in culture collections External links

[Ref.: #6544]

Culture collection no.

DSM 16641, ATCC 33297, CCUG 12267, CIP 104724, NCTC 11401

[Ref.: #76182]

SI-ID 40452

Literature:

	Title	Authors	Journal	DOI	Year
Genetics	Sequence and genome context analysis of a new molecular class D beta-lactamase gene from Legionella pneumophila.	Avison MB, Simm AM	J Antimicrob Chemother	10.1093/jac/dkf135	2002	*
Enzymology	Characterization of OXA-29 from Legionella (Fluoribacter) gormanii: molecular class D beta-lactamase with unusual properties.	Franceschini N, Boschi L, Pollini S, Herman R, Perilli M, Galleni M, Frere JM, Amicosante G, Rossolini GM	Antimicrob Agents Chemother	10.1128/AAC.45.12.3509-3516.2001	2001	*
Enzymology	Biochemical characterization of the FEZ-1 metallo-beta-lactamase of Legionella gormanii ATCC 33297T produced in Escherichia coli.	Mercuri PS, Bouillenne F, Boschi L, Lamotte-Brasseur J, Amicosante G, Devreese B, van Beeumen J, Frere JM, Rossolini GM, Galleni M	Antimicrob Agents Chemother	10.1128/AAC.45.4.1254-1262.2001	2001	*
Enzymology	The Legionella (Fluoribacter) gormanii metallo-beta-lactamase: a new member of the highly divergent lineage of molecular-subclass B3 beta-lactamases.	Boschi L, Mercuri PS, Riccio ML, Amicosante G, Galleni M, Frere JM, Rossolini GM	Antimicrob Agents Chemother	10.1128/AAC.44.6.1538-1543.2000	2000	*
	Distribution of Secretion Systems in the Genus Legionella and Its Correlation with Pathogenicity.	Qin T, Zhou H, Ren H, Liu W	Front Microbiol	10.3389/fmicb.2017.00388	2017	*

References

#6544

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16641

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#38670 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#76182

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID40452.1 )

#119712 Collection of Institut Pasteur ; Curators of the CIP; CIP 104724
* These data were automatically processed and therefore are not curated

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Search for species Legionella gormanii in external resources:
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Fluoribacter gormanii strain NCTC11401

Fluoribacter gormanii ATCC 33297

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