Apilactobacillus ozensis (DSM 23829, JCM 17196, Mizu2-1)

Name: Apilactobacillus ozensis (DSM 23829, JCM 17196, Mizu2-1)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6699

Type strain

Strain Designation Mizu2-1

Culture col. no. DSM 23829 JCM 17196 Mizu2-1 LMG 29536

NCBI tax ID(s) 866801

Special Collections DSMZ JCM

Links

Apilactobacillus ozensis Mizu2-1 is a microaerophile, mesophilic prokaryote that was isolated from Inula ciliaris var. glandulosa, a chrysanthemum.

microaerophile mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 379062

LMG 29536,DSM 23829,JCM 17196

@ref 20215

Last LPSN update 2025-12-16 09:48:23

Domain Bacillati

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Lactobacillaceae

Genus Apilactobacillus

Species Apilactobacillus ozensis

Full scientific name Apilactobacillus ozensis (Kawasaki et al. 2011) Zheng et al. 2020

Synonyms (1)

Lactobacillus ozensis

BacDive ID	Other strains from **Apilactobacillus ozensis** (4)	Type strain
161963	A. ozensis JCM 17197
161964	A. ozensis JCM 17198
161965	A. ozensis JCM 17199
161966	A. ozensis JCM 17200

Morphology

Cell morphology

@ref	Motility	Confidence	Gram stain
125438		91.5
125438		92.777	positive

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
17392	MRS MEDIUM (DSMZ Medium 11)		Medium recipe at MediaDive	Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
17392	positive	growth	30	mesophilic
67770	positive	growth	30	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
17392	microaerophile
125439	obligate aerobe	98.5

Spore formation

@ref	Spore formation	Confidence
125438		90.759

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	-	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	4853 ChEBI	esculin	-	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	-	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	-	builds acid from	from API 50CH acid
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	ppGpp biosynthesis	100	4 of 4
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	formaldehyde oxidation	100	3 of 3
66794	coenzyme A metabolism	100	4 of 4
66794	ethanol fermentation	100	2 of 2
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	teichoic acid biosynthesis	100	1 of 1
66794	acetoin degradation	100	3 of 3
66794	palmitate biosynthesis	100	22 of 22
66794	folate polyglutamylation	100	1 of 1
66794	threonine metabolism	90	9 of 10
66794	peptidoglycan biosynthesis	80	12 of 15
66794	glycine betaine biosynthesis	80	4 of 5
66794	chorismate metabolism	77.78	7 of 9
66794	aspartate and asparagine metabolism	77.78	7 of 9
66794	phenylalanine metabolism	76.92	10 of 13
66794	C4 and CAM-carbon fixation	75	6 of 8
66794	acetate fermentation	75	3 of 4
66794	purine metabolism	72.34	68 of 94
66794	NAD metabolism	72.22	13 of 18
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	pyrimidine metabolism	68.89	31 of 45
66794	valine metabolism	66.67	6 of 9
66794	cyanate degradation	66.67	2 of 3
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	photosynthesis	64.29	9 of 14
66794	ketogluconate metabolism	62.5	5 of 8
66794	gluconeogenesis	62.5	5 of 8
66794	glycogen metabolism	60	3 of 5
66794	degradation of sugar alcohols	56.25	9 of 16
66794	serine metabolism	55.56	5 of 9
66794	CO2 fixation in Crenarchaeota	55.56	5 of 9
66794	non-pathway related	55.26	21 of 38
66794	pentose phosphate pathway	54.55	6 of 11
66794	glycolysis	52.94	9 of 17
66794	butanoate fermentation	50	2 of 4
66794	lactate fermentation	50	2 of 4
66794	tetrahydrofolate metabolism	50	7 of 14
66794	glycolate and glyoxylate degradation	50	3 of 6
66794	suberin monomers biosynthesis	50	1 of 2
66794	glutamate and glutamine metabolism	50	14 of 28
66794	sulfopterin metabolism	50	2 of 4
66794	vitamin B1 metabolism	46.15	6 of 13
66794	oxidative phosphorylation	46.15	42 of 91
66794	d-mannose degradation	44.44	4 of 9
66794	ubiquinone biosynthesis	42.86	3 of 7
66794	propanol degradation	42.86	3 of 7
66794	mevalonate metabolism	42.86	3 of 7
66794	reductive acetyl coenzyme A pathway	42.86	3 of 7
66794	methionine metabolism	42.31	11 of 26
66794	flavin biosynthesis	40	6 of 15
66794	propionate fermentation	40	4 of 10
66794	Entner Doudoroff pathway	40	4 of 10
66794	arachidonate biosynthesis	40	2 of 5
66794	leucine metabolism	38.46	5 of 13
66794	lysine metabolism	38.1	16 of 42
66794	alanine metabolism	37.93	11 of 29
66794	isoleucine metabolism	37.5	3 of 8
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	37.5	3 of 8
66794	proline metabolism	36.36	4 of 11
66794	tyrosine metabolism	35.71	5 of 14
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	degradation of aromatic, nitrogen containing compounds	33.33	4 of 12
66794	selenocysteine biosynthesis	33.33	2 of 6
66794	arginine metabolism	33.33	8 of 24
66794	IAA biosynthesis	33.33	1 of 3
66794	octane oxidation	33.33	1 of 3
66794	sphingosine metabolism	33.33	2 of 6
66794	degradation of pentoses	32.14	9 of 28
66794	isoprenoid biosynthesis	30.77	8 of 26
66794	glycine metabolism	30	3 of 10
66794	lipid metabolism	29.03	9 of 31
66794	tryptophan metabolism	28.95	11 of 38
66794	cyclohexanol degradation	25	1 of 4
66794	carnitine metabolism	25	2 of 8
66794	glycogen biosynthesis	25	1 of 4
66794	urea cycle	23.08	3 of 13
66794	lipid A biosynthesis	22.22	2 of 9
66794	degradation of hexoses	22.22	4 of 18
66794	cysteine metabolism	22.22	4 of 18
66794	nitrate assimilation	22.22	2 of 9
66794	citric acid cycle	21.43	3 of 14
66794	glutathione metabolism	21.43	3 of 14

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
17392	-	-	-	-	-	-	-	-	-	-	-	+	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Plants	#Herbaceous plants (Grass,Crops)

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent
17392	Inula ciliaris var. glandulosa, a chrysanthemum	Inula ciliaris var. glandulosa	Gunma prefecture, Oze National Park	Japan	JPN	Asia
67770	Mountain flowers		Oze National Park	Japan	JPN	Asia

Taxonmaps

69479

Global distribution of 16S sequence LC519864 (>99% sequence identity) for Apilactobacillus ozensis subclade from Microbeatlas

Aquatic Samples	88
Soil Samples	51
Animal Samples	2085
Plant Samples	199
Total Samples	2423

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
17392	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM143599v1 assembly for Apilactobacillus ozensis DSM 23829 = JCM 17196	scaffold	GCA_001435995	1423781.4	2660238120	1423781	68.6
67770	ASM131148v1 assembly for Apilactobacillus ozensis DSM 23829 = JCM 17196	contig	GCA_001311485	1423781.3	2675903667	1423781	27.72

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
17392	Lactobacillus ozensis gene for 16S rRNA, partial sequence, strain: Mizu2-1	AB572588	1541		1423781
67770	Lactobacillus ozensis JCM 17196 gene for 16S rRNA, partial sequence	LC519864	1511		866801

GC content

@ref	GC-content (mol%)	Method
17392	41	high performance liquid chromatography (HPLC)
67770	31.9	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Apilactobacillus ozensis DSM 23829 = JCM 17196
NCBI: GCA_001435995

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	74.90	no
125439	motility	BacteriaNetⓘ	yes	53.20	no
125439	gram_stain	BacteriaNetⓘ	positive	73.00	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.50	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Lactobacillus ozensis DSM 23829 = JCM 17196
PATRIC: 1423781.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	92.78	no
125438	anaerobic	anaerobicⓘ	no	84.24	yes
125438	spore-forming	spore-formingⓘ	no	90.76	no
125438	aerobic	aerobicⓘ	no	88.30	yes
125438	thermophilic	thermophileⓘ	no	91.56	no
125438	flagellated	motile2+ⓘ	no	91.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Molecular characterization and bioinformatics studies of a lipase from Bacillus thermoamylovorans BHK67.	Sharma A, Meena KR, Kanwar SS	Int J Biol Macromol	10.1016/j.ijbiomac.2017.10.092	2017
Phylogeny	Lactobacillus kosoi sp. nov., a fructophilic species isolated from koso, a Japanese sugar-vegetable fermented beverage.	Chiou TY, Suda W, Oshima K, Hattori M, Matsuzaki C, Yamamoto K, Takahashi T	Antonie Van Leeuwenhoek	10.1007/s10482-018-1019-7	2018
Phylogeny	Lactobacillus ozensis sp. nov., isolated from mountain flowers.	Kawasaki S, Kurosawa K, Miyazaki M, Sakamoto M, Ohkuma M, Niimura Y	Int J Syst Evol Microbiol	10.1099/ijs.0.027847-0	2010

References

#17392	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23829
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Apilactobacillus ozensis (DSM 23829, JCM 17196, Mizu2-1)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Pathway annotation

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_001435995

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media