Name: Fructilactobacillus florum F9-1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6696

Type strain:

Species: Fructilactobacillus florum

Strain Designation: F9-1

Culture col. no.: DSM 22689, JCM 16035, NRIC 0771

Strain history: A. Endo F9-1.

NCBI tax ID(s): 640331 (species)

SI-ID 371014

DSM 22689, JCM 16035, LMG 26406

Other strains from Fructilactobacillus florum

F. florum JCM 16036
F. florum JCM 16037

Section

Fructilactobacillus florum F9-1 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from peony .

Gram-positive
rod-shaped
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Fructilactobacillus*
	Species	**Fructilactobacillus florum**
	Full Scientific Name (LPSN)	Fructilactobacillus florum (Endo et al. 2010) Zheng et al. 2020

Synonym

Lactobacillus florum

Information on morphological and physiological properties Morphology

[Ref.: #29704]	Gram stain	positive

[Ref.: #29704]	Cell length	1.5-7 µm

[Ref.: #29704]	Cell width	0.8 µm

[Ref.: #29704]	Cell shape	rod-shaped

[Ref.: #29704]	Motility	no

[Ref.: #29704]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16464]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #16464]

Culture medium growth

[Ref.: #16464]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16464]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #16464]	growth	30 °C
[Ref.: #29704]	growth	15 °C
[Ref.: #29704]	optimum	15 °C
[Ref.: #67770]	growth	30 °C

[Ref.: #16464]	Temperature range	mesophilic
[Ref.: #29704]	Temperature range	psychrophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #29704]			optimum	7
[Ref.: #29704]			growth	04-08

Information on physiology and metabolism Physiology and metabolism

[Ref.: #16464]	Oxygen tolerance	microaerophile
[Ref.: #29704]	Oxygen tolerance	facultative anaerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #29704]		NaCl		growth	5	%
[Ref.: #29704]		NaCl		optimum	5	%

[Ref.: #29704]

Observation

aggregates in chains

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #29704]	fructose ChEBI	+	carbon source
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #29704]	glucose ChEBI	+	carbon source
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #29704]		catalase	+	1.11.1.6

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #16464]	14751	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16464]	Sample type/isolated from	peony (Paeonia suffruticosa)
[Ref.: #16464]	Host species	Paeonia suffruticosa
[Ref.: #16464]	Geographic location (country and/or sea, region)	Western Cape, Stellenbosch
[Ref.: #16464]	Country	South Africa
[Ref.: #16464]	Country ISO 3 Code	ZAF
[Ref.: #16464]	Continent	Africa

[Ref.: #67770]	Sample type/isolated from	Peony and bietou flowers
[Ref.: #67770]	Geographic location (country and/or sea, region)	Stellenbosch and Hermanus area, Western Cape
[Ref.: #67770]	Country	South Africa
[Ref.: #67770]	Country ISO 3 Code	ZAF
[Ref.: #67770]	Continent	Africa
* marker position based on {}

Isolation sources categories

#Host

#Plants

#Shrub (Scrub)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC483564 (>99% sequence identity) for Fructilactobacillus florum subclade from Microbeatlas

Aquatic Samples	473
Soil Samples	76
Animal Samples	1951
Plant Samples	101
Total Samples	2601

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16464]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Lactobacillus florum gene for 16S ribosomal RNA, partial sequence	LC483564	1516	ENA	640331 tax ID
[Ref.: #16464]	Lactobacillus florum gene for 16S rRNA, partial sequence	AB498045	1507	ENA	1423745 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Fructilactobacillus florum DSM 22689 = JCM 16035	GCA_001436645	contig	GenBank	1423745 tax ID
[Ref.: #67770]	Fructilactobacillus florum DSM 22689 = JCM 16035	GCA_001311215	contig	GenBank	1423745 tax ID
[Ref.: #66792]	Lactobacillus florum DSM 22689 = JCM 16035	1423745.4	wgs	PATRIC	1423745 tax ID	*
[Ref.: #66792]	Lactobacillus florum DSM 22689 = JCM 16035	1423745.3	wgs	PATRIC	1423745 tax ID	*
[Ref.: #66792]	Fructilactobacillus florum DSM 22689	2657244926	draft	JGI IMG/M	1423745 tax ID	*
[Ref.: #66792]	Fructilactobacillus florum JCM 16035	2728369675	draft	JGI IMG/M	1423745 tax ID	*

[Ref.: #16464]	GC-content	42 mol%	high performance liquid chromatography (HPLC)
[Ref.: #29704]	GC-content	42 mol%
[Ref.: #67770]	GC-content	41.1 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Lactobacillus florum DSM 22689 = JCM 16035 PATRIC: 1423745.4
[Ref.: #69480]	motile	no	96.364	yes
[Ref.: #69480]	gram-positive	yes	95.301	no
[Ref.: #69480]	anaerobic	no	90.284	yes
[Ref.: #69480]	halophile	yes	89.917	no
[Ref.: #69480]	spore-forming	no	97.41	no
[Ref.: #69480]	glucose-util	yes	88.806	yes
[Ref.: #69480]	thermophile	no	98.31	yes
[Ref.: #69480]	aerobic	no	95.439	yes
[Ref.: #69480]	flagellated	no	98.809	yes
[Ref.: #69480]	glucose-ferment	yes	80.221	no

Availability in culture collections External links

[Ref.: #16464]

Culture collection no.

DSM 22689, JCM 16035, NRIC 0771

[Ref.: #76170]

SI-ID 371014

Literature:

	Title	Authors	Journal	DOI	Year
Phylogeny	Lactobacillus ixorae sp. nov., isolated from a flower (West-Indian jasmine).	Techo S, Miyashita M, Shibata C, Tanaka N, Wisetkhan P, Visessanguan W, Tanasupawat S	Int J Syst Evol Microbiol	10.1099/ijsem.0.001547	2016	*
Phylogeny	Identification and characterization of Lactobacillus florum strains isolated from South African grape and wine samples.	Mtshali PS, Divol B, du Toit M	Int J Food Microbiol	10.1016/j.ijfoodmicro.2011.10.023	2011	*
Phylogeny	Lactobacillus florum sp. nov., a fructophilic species isolated from flowers.	Endo A, Futagawa-Endo Y, Sakamoto M, Kitahara M, Dicks LMT	Int J Syst Evol Microbiol	10.1099/ijs.0.019067-0	2009	*
	Apple Pomace as a Sustainable Substrate in Sourdough Fermentation.	Martau GA, Teleky BE, Ranga F, Pop ID, Vodnar DC	Front Microbiol	10.3389/fmicb.2021.742020	2021	*
	Biofunctional soy-based sourdough for improved rheological properties during storage.	Teleky BE, Martau GA, Ranga F, Pop ID, Vodnar DC	Sci Rep	10.1038/s41598-022-22551-z	2022	*

References

#16464

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22689

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#29704

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26087 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#76170

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID371014.1 )

#26087

IJSEM 2478 2010 ( DOI 10.1099/ijs.0.019067-0 , PubMed 19965998 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Fructilactobacillus florum in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF

Lactobacillus florum DSM 22689 = JCM 16035

Change proposal

Change proposal