Name: Lactobacillus equicursoris DI70
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 6693

Type strain:

Species: Lactobacillus equicursoris

Strain Designation: DI70

Culture col. no.: DSM 19284, CIP 110162, JCM 14600, LMG 30910

Strain history: CIP <- 2010, DSMZ <- H. Morita and H. Shiono, Azabu Univ., Sagamihara, Japan: strain DI70

NCBI tax ID(s): 1293597 (strain), 420645 (species)

SI-ID 366446

DSM 19284, JCM 14600, CIP 110162

Special Collections

Other strains from Lactobacillus equicursoris

Section

When using BacDive for research please cite our paper

Lactobacillus equicursoris DI70 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from healthy thoroughbred racehorse.

Gram-positive
rod-shaped
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Lactobacillus*
	Species	**Lactobacillus equicursoris**
	Full Scientific Name (LPSN)	Lactobacillus equicursoris Morita et al. 2010

Information on morphological and physiological properties Morphology

[Ref.: #29241]	Gram stain	positive
[Ref.: #119118]	Gram stain	positive
[Ref.: #125438]	Gram stain	positive Confidence in %93.713

[Ref.: #29241]	Cell length	20 µm

[Ref.: #29241]	Cell width	0.75 µm

[Ref.: #29241]	Cell shape	rod-shaped
[Ref.: #119118]	Cell shape	rod-shaped

[Ref.: #29241]	Motility	no
[Ref.: #119118]	Motility	no
[Ref.: #125438]	Motility	no Confidence in %91

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7996]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #7996]

Culture medium growth

[Ref.: #7996]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7996]

Culture medium composition

expand / minimize

[Ref.: #37923]

Culture medium

MEDIUM 40- for Lactobacillus and Leuconostoc

[Ref.: #37923]

Culture medium growth

[Ref.: #37923]

Culture medium composition

expand / minimize

[Ref.: #119118]

Culture medium

CIP Medium 40

[Ref.: #119118]

Culture medium growth

[Ref.: #119118]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #7996]	growth	37 °C
[Ref.: #29241]	growth	30-45 °C
[Ref.: #29241]	optimum	35 °C
[Ref.: #37923]	growth	37 °C
[Ref.: #67770]	growth	37 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #7996]	Oxygen tolerance	microaerophile
[Ref.: #29241]	Oxygen tolerance	anaerobe
[Ref.: #119118]	Oxygen tolerance	facultative anaerobe

[Ref.: #125439]

Ability of spore formation

no Confidence in %98.6

[Ref.: #7996]	Murein short key	A11.31
[Ref.: #7996]	Murein types	A4alpha L-Lys-D-Asp

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #29241]	cellobiose ChEBI	+	carbon source
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #29241]	fructose ChEBI	+	carbon source
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #29241]	galactose ChEBI	+	carbon source
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #29241]	glucose ChEBI	+	carbon source
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #29241]	lactose ChEBI	+	carbon source
[Ref.: #29241]	mannitol ChEBI	+	carbon source
[Ref.: #29241]	mannose ChEBI	+	carbon source
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #119118]	nitrate ChEBI	-	reduction
[Ref.: #119118]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #29241]	raffinose ChEBI	+	carbon source
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #29241]	salicin ChEBI	+	carbon source
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #119118]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #119118]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #119118]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #119118]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #7996]	15191	-	-	-	-	-	-	-	-	-	-	+	+	+	+	-	-	-	-	-	-	-	-	+	+	+	+	+	+	+	+	-	+	-	-	-	+	+	-	-	+	-	-	-	-	-	-	-	-	-	-
[Ref.: #119118]	11710	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #119118]	12401	-	+	+	-	-	-	-	-	-	-	+	+	+	+	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7996]	Sample type/isolated from	healthy thoroughbred racehorse
[Ref.: #7996]	Geographic location (country and/or sea, region)	Shiga
[Ref.: #7996]	Country	Japan
[Ref.: #7996]	Country ISO 3 Code	JPN
[Ref.: #7996]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Feces of a thoroughbred racehorse
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia

[Ref.: #119118]	Sample type/isolated from	Thoroughbred racehorse, faeces
[Ref.: #119118]	Geographic location (country and/or sea, region)	Shiga
[Ref.: #119118]	Country	Japan
[Ref.: #119118]	Country ISO 3 Code	JPN
[Ref.: #119118]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host

#Mammals

#Equidae (Horse)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC480805 (>99% sequence identity) for Lactobacillus from Microbeatlas

Aquatic Samples	2848
Soil Samples	1469
Animal Samples	38003
Plant Samples	749
Total Samples	43069

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7996]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #119118]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7996]	Lactobacillus equicursoris gene for 16S ribosomal RNA, partial sequence	AB290830	1486	GenBank ENA	1293597 tax ID
[Ref.: #67770]	Lactobacillus equicursoris JCM 14600 gene for 16S ribosomal RNA, partial sequence	LC480805	1481	GenBank ENA	1293597 tax ID
[Ref.: #124043]	Lactobacillus equicursoris partial 16S rRNA gene, type strain CIP 110162T	FR775894	1548	GenBank ENA	1293597 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Lactobacillus equicursoris DSM 19284 = JCM 14600 = CIP 110162	GCA_001311765	contig	GenBank ENA	1293597 tax ID
[Ref.: #67770]	Lactobacillus equicursoris DSM 19284 = JCM 14600 = CIP 110162 type strain:CIP 110162	GCA_000312645	contig	GenBank ENA	1293597 tax ID
[Ref.: #67770]	Lactobacillus equicursoris DSM 19284 = JCM 14600 = CIP 110162	GCA_001434815	scaffold	GenBank ENA	1293597 tax ID
[Ref.: #66792]	Lactobacillus equicursoris DSM 19284 = JCM 14600 = CIP 110162	1293597.3	wgs	PATRIC	1293597 tax ID	*
[Ref.: #66792]	Lactobacillus equicursoris CIP 110162	2537562121	draft	JGI IMG/M	1293597 tax ID	*
[Ref.: #66792]	Lactobacillus equicursoris DSM 19284	2663763010	draft	JGI IMG/M	1293597 tax ID	*

[Ref.: #7996]	GC-content	48.3 mol%
[Ref.: #67770]	GC-content	48.3 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67770]	GC-content	47.7 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Model	Trait	Prediction	Confidence in %	In training data
	Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Lactobacillus equicursoris CIP 110162 PATRIC: 1211775.3
[Ref.: #125438]	gram-positive	gram-positiveⓘ	yes	93.713	no
[Ref.: #125438]	anaerobic	anaerobicⓘ	no	75.5	no
[Ref.: #125438]	spore-forming	spore-formingⓘ	no	85.493	no
[Ref.: #125438]	aerobic	aerobicⓘ	no	96.694	yes
[Ref.: #125438]	thermophile	thermophilicⓘ	no	96.5	yes
[Ref.: #125438]	motile2+	flagellatedⓘ	no	91	yes

	Model	Trait	Prediction	Confidence in %
	Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Lactobacillus equicursoris DSM 19284 = JCM 14600 = CIP 110162 NCBI: GCA_001434815
[Ref.: #125439]	BacteriaNet	oxygen_toleranceⓘ	anaerobe	89.5
[Ref.: #125439]	BacteriaNet	gram_stainⓘ	negative	87.9
[Ref.: #125439]	BacteriaNet	motilityⓘ	no	73.1
[Ref.: #125439]	BacteriaNet	spore_formationⓘ	no	98.6

Availability in culture collections External links

[Ref.: #7996]

Culture collection no.

DSM 19284, CIP 110162, JCM 14600, LMG 30910

[Ref.: #76167]

SI-ID 366446

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Lactobacillus porci sp. nov., isolated from small intestine of a swine.	Kim JS, Choe H, Kim KM, Lee YR, Rhee MS, Park DS	Int J Syst Evol Microbiol	10.1099/ijsem.0.002949	2018	*
Genetics	Draft Genome Sequences of Lactobacillus equicursoris CIP 110162T and Lactobacillus sp. Strain CRBIP 24.137, Isolated from Thoroughbred Racehorse Feces and Human Urine, Respectively.	Cousin S, Loux V, Ma L, Creno S, Clermont D, Bizet C, Bouchier C	Genome Announc	10.1128/genomeA.00663-13	2013	*
Phylogeny	Lactobacillus equicursoris sp. nov., isolated from the faeces of a thoroughbred racehorse.	Morita H, Shimazu M, Shiono H, Toh H, Nakajima F, Akita H, Takagi M, Takami H, Murakami M, Masaoka T, Tanabe S, Hattori M	Int J Syst Evol Microbiol	10.1099/ijs.0.009290-0	2009	*

References

#7996

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19284

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#29241

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25661 (see below)

#37923 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#76167

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID366446.1 )

#119118 Collection of Institut Pasteur ; Curators of the CIP; CIP 110162

#124043

Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .

#125438

Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )

#125439

Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .

#25661

IJSEM 109 2010 ( DOI 10.1099/ijs.0.009290-0 , PubMed 19648335 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Lactobacillus equicursoris in external resources:
SILVA
BRENDA
PANGAEA
GBIF

Lactobacillus equicursoris CIP 110162

Lactobacillus equicursoris DSM 19284 = JCM 14600 = CIP 110162

Change proposal

Change proposal