Name: Lentilactobacillus otakiensis DSM 19908
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6685

Type strain:

Species: Lentilactobacillus otakiensis

Culture col. no.: DSM 19908, JCM 15040, NRIC 0742, YIT 11163, CIP 110027

Strain history: CIP <- 2009, JCM <- 2007, K. Watanabe, Yakult Central Inst. Microbiol. Res., Tokyo, Japan: strain YIT 11163

NCBI tax ID(s): 481720 (species)

SI-ID 362307

DSM 19908, JCM 15040, CIP 110027

Section

Lentilactobacillus otakiensis DSM 19908 is a microaerophile, mesophilic bacterium that was isolated from non-salted pickle solution used in production of sunki .

mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Lentilactobacillus*
	Species	**Lentilactobacillus otakiensis**
	Full Scientific Name (LPSN)	Lentilactobacillus otakiensis (Watanabe et al. 2009) Zheng et al. 2020

Synonym

Lactobacillus otakiensis

Information on morphological and physiological properties Morphology

[Ref.: #121466]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #121466]	Cell shape	rod-shaped

[Ref.: #121466]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %95.646

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8380]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #8380]

Culture medium growth

[Ref.: #8380]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8380]

Culture medium composition

expand / minimize

[Ref.: #40627]

Culture medium

MEDIUM 20 - for Anaerobic bacteria

[Ref.: #40627]

Culture medium growth

[Ref.: #40627]

Culture medium composition

expand / minimize

[Ref.: #121466]

Culture medium

CIP Medium 20

[Ref.: #121466]

Culture medium growth

[Ref.: #121466]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #121466]

Culture medium

CIP Medium 40

[Ref.: #121466]

Culture medium growth

[Ref.: #121466]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #8380]		growth	30 °C
[Ref.: #40627]		growth	37 °C
[Ref.: #67770]		growth	30 °C
[Ref.: #121466]		growth	15-37 °C
[Ref.: #121466]	no	growth	45 °C

[Ref.: #8380]	Temperature range	mesophilic
[Ref.: #40627]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #121466]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #8380]	Oxygen tolerance	microaerophile
[Ref.: #121466]	Oxygen tolerance	facultative anaerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.996

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #121466]	nitrate ChEBI	-	reduction
[Ref.: #121466]	nitrate ChEBI	+	respiration
[Ref.: #121466]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #121466]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #121466]	alcohol dehydrogenase	+	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	+	3.2.1.31	* from API zym
[Ref.: #121466]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #121466]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #121466]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #121466]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #8380]	14408	-	-	-	-	+	+	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121466]	12301	-	+	-	+	-	+	-	-	-	-	+	+	+	+	+	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8380]	Sample type/isolated from	non-salted pickle solution used in production of sunki (Japanese traditional fermented leaves and stems of red turnips)
[Ref.: #8380]	Geographic location (country and/or sea, region)	Kiso county, nagano prefecture
[Ref.: #8380]	Country	Japan
[Ref.: #8380]	Country ISO 3 Code	JPN
[Ref.: #8380]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Sunki, traditional Japanese pickles
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia

[Ref.: #121466]	Sample type/isolated from	Sunki, non-salted pickle solution
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Vegetable (incl. Grains)
#Engineered	#Food production	#Fermented

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC480801 (>99% sequence identity) for Lentilactobacillus from Microbeatlas

Aquatic Samples	1599
Soil Samples	1041
Animal Samples	7375
Plant Samples	556
Total Samples	10571

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8380]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #121466]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #8380]	Lactobacillus otakiensis gene for 16S rRNA, partial sequence, strain: YIT 11163 (= NRIC 0742, = JCM 15040, = DSM 19908)	AB366386	1566	ENA	481720 tax ID
[Ref.: #67770]	Lactobacillus otakiensis JCM 15040 gene for 16S ribosomal RNA, partial sequence	LC480801	1509	ENA	481720 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Lentilactobacillus otakiensis DSM 19908 = JCM 15040	GCA_000415925	contig	GenBank	1423780 tax ID
[Ref.: #67770]	Lentilactobacillus otakiensis DSM 19908 = JCM 15040	GCA_001311395	contig	GenBank	1423780 tax ID
[Ref.: #67770]	Lentilactobacillus otakiensis DSM 19908 = JCM 15040	GCA_001434145	scaffold	GenBank	1423780 tax ID
[Ref.: #66792]	Lactobacillus otakiensis JCM 15040	1291737.4	wgs	PATRIC	1423780 tax ID	*
[Ref.: #66792]	Lentilactobacillus otakiensis DSM 19908	2663763512	draft	JGI IMG/M	1423780 tax ID	*
[Ref.: #66792]	Lentilactobacillus otakiensis JCM 15040	2728369684	draft	JGI IMG/M	1423780 tax ID	*

[Ref.: #8380]	GC-content	39.6 mol%
[Ref.: #67770]	GC-content	42.4 mol%	genome sequence analysis
[Ref.: #67770]	GC-content	39.6 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Lentilactobacillus otakiensis DSM 19908 = JCM 15040 DSM 19908 NCBI: GCA_001434145.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Lactobacillus otakiensis DSM 19908 = JCM 15040 PATRIC: 1423780.4
[Ref.: #69480]	motile	no	95.95	no
[Ref.: #69480]	gram-positive	yes	97.233	no
[Ref.: #69480]	anaerobic	no	94.385	yes
[Ref.: #69480]	halophile	yes	93.847	no
[Ref.: #69480]	spore-forming	no	94.303	no
[Ref.: #69480]	thermophile	no	99.413	yes
[Ref.: #69480]	glucose-util	yes	86.577	no
[Ref.: #69480]	aerobic	no	94.002	no
[Ref.: #69480]	flagellated	no	98.597	no
[Ref.: #69480]	glucose-ferment	yes	89.004	no

Availability in culture collections External links

[Ref.: #8380]

Culture collection no.

DSM 19908, JCM 15040, NRIC 0742, YIT 11163, CIP 110027

[Ref.: #76159]

SI-ID 362307

Literature:

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Identification, purification, and characterization of a novel amino acid racemase, isoleucine 2-epimerase, from Lactobacillus species.	Mutaguchi Y, Ohmori T, Wakamatsu T, Doi K, Ohshima T	J Bacteriol	10.1128/JB.00709-13	2013	*
Genetics	Draft Genome Sequence of D-Branched-Chain Amino Acid Producer Lactobacillus otakiensis JCM 15040T, Isolated from a Traditional Japanese Pickle.	Doi K, Mori K, Mutaguchi Y, Tashiro K, Fujino Y, Ohmori T, Kuhara S, Ohshima T	Genome Announc	10.1128/genomeA.00546-13	2013	*
Phylogeny	Lactobacillus kisonensis sp. nov., Lactobacillus otakiensis sp. nov., Lactobacillus rapi sp. nov. and Lactobacillus sunkii sp. nov., heterofermentative species isolated from sunki, a traditional Japanese pickle.	Watanabe K, Fujimoto J, Tomii Y, Sasamoto M, Makino H, Kudo Y, Okada S	Int J Syst Evol Microbiol	10.1099/ijs.0.004689-0	2009	*

References

#8380

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19908

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#40627 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#76159

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID362307.1 )

#121466 Collection of Institut Pasteur ; Curators of the CIP; CIP 110027
* These data were automatically processed and therefore are not curated

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Lentilactobacillus otakiensis DSM 19908 = JCM 15040 DSM 19908

Lactobacillus otakiensis DSM 19908 = JCM 15040

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