Levilactobacillus acidifarinae (CCUG 50162, DSM 19394, CCM 7240)

Name: Levilactobacillus acidifarinae (CCUG 50162, DSM 19394, CCM 7240)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6661

Type strain

Strain Designation R-19065, R-16065

Culture col. no. CCUG 50162 DSM 19394 CCM 7240 LMG 22200 R-19065 5 more

NCBI tax ID(s) 1423715 267364

Special Collections DSMZ CCUG JCM CIP Literature strains

History DSMZ <- CCM <- M. Vancanneyt, R-19065 <- P. Neysens JCM <-- LMG 22200 <-- M. Vancanneyt R-19065 <-- P. Neysens. CIP <- 2005, CCM <- M. Vancanneyt, LMG <- P Neysens, IMDO, Vrije Univ., Brussels, Belgium: strain R-16065

Links

Levilactobacillus acidifarinae R-19065 is a microaerophile, Gram-positive, rod-shaped bacterium that was isolated from artisanal wheat sourdough.

Gram-positive rod-shaped microaerophile genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 10207

LMG 22200,CCM 7240,CCUG 50162,DSM 19394,JCM 15949,CIP 108702

@ref 20215

Last LPSN update 2026-05-29 08:26:27

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Lactobacillaceae

Genus Levilactobacillus

Species Levilactobacillus acidifarinae

Full scientific name Levilactobacillus acidifarinae (Vancanneyt et al. 2005) Zheng et al. 2020

Synonyms (1)

Lactobacillus acidifarinae

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Confidence
31309	positive	11 µm	1 µm	rod-shaped
115998	positive			rod-shaped
125438					90.5
125438	positive				92.376

Pigmentation

31309

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
8088	PEDIOCOCCUS DAMNOSUS MEDIUM (DSMZ Medium 231)	Medium recipe at MediaDive	Name: PEDIOCOCCUS DAMNOSUS MEDIUM (DSMZ Medium 231) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Yeast extract 5.0 g/l Na-acetate 5.0 g/l K2HPO4 2.0 g/l (NH4)3 citrate 2.0 g/l Tween 80 1.0 g/l Cysteine hydrochloride 0.5 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
38291	MEDIUM 40- for Lactobacillus and Leuconostoc		Distilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
115998	CIP Medium 40	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
8088	positive	growth	30
31309	positive	optimum	7.5
38291	positive	growth	37
58765	positive	growth	37
67770	positive	growth	30
115998	positive	growth	10-37
115998	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
8088	microaerophile
31309	facultative anaerobe
58765	anaerobe
115998	facultative anaerobe
125439	facultative anaerobe	94.196

Spore formation

31309

Spore formationno

Halophily

@ref	Salt	Growth	Tested relation	Concentration
31309	NaCl	positive	growth	<5 %
31309	NaCl	positive	optimum	2.5 %

Observation

31309

Observationaggregates in chains

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
31309	22599 ChEBI	arabinose	+	carbon source
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
31309	28757 ChEBI	fructose	+	carbon source
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
31309	17234 ChEBI	glucose	+	carbon source
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	+	builds acid from	from API 50CH acid
31309	18403 ChEBI	L-arabitol	+	carbon source
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
31309	17306 ChEBI	maltose	+	carbon source
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
31309	29864 ChEBI	mannitol	+	carbon source
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
115998	17632 ChEBI	nitrate	-	reduction
115998	17632 ChEBI	nitrate	+	respiration
115998	16301 ChEBI	nitrite	-	reduction
68371	0 ChEBI	Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371	0 ChEBI	Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
31309	33942 ChEBI	ribose	+	carbon source
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid
31309	18222 ChEBI	xylose	+	carbon source

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
115998	15688 ChEBI	acetoin	-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
115998	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	+	3.2.1.31	from API zym
115998	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	-		from API zym
68382	leucine arylamidase	-	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
115998	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
115998	ornithine decarboxylase	-	4.1.1.17
115998	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
115998	-	+	-	-	-	-	-	-	-	-	+	+	+	+	+	-	+	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
8088	-	-	-	-	+	+	+	-	-	-	+	+	+	-	-	-	-	-	+	-	-	-	+	-	-	+	-	-	+	+/-	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	+	-	+	-	-
115998	not determinedn.d.	-	-	-	+	+/-	+/-	-	-	-	+/-	+	+	-	-	-	-	-	+/-	-	-	-	+	-	-	-	-	-	+	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	+/-	-	+/-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Food production	#Food

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent	Isolation date
8088	artisanal wheat sourdough	Belgium	BEL	Europe
58765	Wheat sourdough	Belgium	BEL	Europe
67770	Wheat sourdough
115998	Food, Wheat sourdough	Belgium	BEL	Europe	2003

Taxonmaps

69479

Global distribution of 16S sequence LC483563 (>99% sequence identity) for Levilactobacillus from Microbeatlas

Aquatic Samples	158
Soil Samples	49
Animal Samples	2008
Plant Samples	170
Total Samples	2385

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
8088	1	Risk group (German classification) According to TRBA 466
115998	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
124043	ASM4243319v1 assembly for Levilactobacillus acidifarinae CCM 7240	scaffold	GCA_042433195			267364	74.1
67770	ASM143483v1 assembly for Levilactobacillus acidifarinae DSM 19394 = JCM 15949	scaffold	GCA_001434835	1423715.3	2700989134	1423715	71.37
67770	ASM799197v1 assembly for Levilactobacillus acidifarinae NBRC 107156	contig	GCA_007991975	267364.3		267364	68.99

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
8088	Lactobacillus acidifarinae 16S rRNA gene, type strain LMG 22200	AJ632158	1518	267364
67770	Lactobacillus acidifarinae gene for 16S ribosomal RNA, partial sequence	LC483563	1508	267364
124043	Lactobacillus acidifarinae gene for 16S rRNA, partial sequence, strain: NBRC 107156.	AB626070	1491	267364
124043	Lactobacillus acidifarinae strain CCM 7240 16S ribosomal RNA gene, partial sequence.	MT760124	1411	267364
124043	Lactobacillus acidifarinae strain CIP 108702 16S-23S ribosomal RNA intergenic spacer and 23S ribosomal RNA gene, partial sequence.	EU161581	791	267364

GC content

@ref	GC-content (mol%)	Method
8088	51
67770	51	high performance liquid chromatography (HPLC)
67770	51.5	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM4243319v1 assembly for Levilactobacillus acidifarinae CCM 7240
NCBI: GCA_042433195

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	94.20	no
125439	gram_stain	BacteriaNetⓘ	variable	54.20	no
125439	motility	BacteriaNetⓘ	no	60.86	no
125439	spore_formation	BacteriaNetⓘ	yes	36.59	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Lactobacillus acidifarinae DSM 19394
PATRIC: 1423715.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	92.38	yes
125438	anaerobic	anaerobicⓘ	no	84.42	no
125438	spore-forming	spore-formingⓘ	no	78.42	yes
125438	aerobic	aerobicⓘ	no	97.16	yes
125438	thermophilic	thermophileⓘ	no	95.00	yes
125438	flagellated	motile2+ⓘ	no	90.50	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Complete genome sequence of Levilactobacillus acidifarinae type strain JCM 15949 (DSM 19394).	Oshiro M, Nakamura K, Sk R, Tashiro Y, Shiwa Y.	Microbiol Resour Announc	10.1128/mra.00374-25	2025
Genetics	Genus-Wide Assessment of Antibiotic Resistance in Lactobacillus spp.	Campedelli I, Mathur H, Salvetti E, Clarke S, Rea MC, Torriani S, Ross RP, Hill C, O'Toole PW.	Appl Environ Microbiol	10.1128/aem.01738-18	2019
Phylogeny	Lactobacillus acidifarinae sp. nov. and Lactobacillus zymae sp. nov., from wheat sourdoughs.	Vancanneyt M, Neysens P, De Wachter M, Engelbeen K, Snauwaert C, Cleenwerck I, Van der Meulen R, Hoste B, Tsakalidou E, De Vuyst L, Swings J	Int J Syst Evol Microbiol	10.1099/ijs.0.63274-0	2005

References

#8088	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19394
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#27624	IJSEM 615 2005 ( DOI 10.1099/ijs.0.63274-0 , PubMed 15774633 )
#31309	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27624
#38291	; Curators of the CIP;
#58765	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 50162
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#115998	Collection of Institut Pasteur ; Curators of the CIP; CIP 108702
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Levilactobacillus acidifarinae (CCUG 50162, DSM 19394, CCM 7240)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite tests

Enzymes

API zym

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_042433195

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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