Lactobacillus brevis 14, Bb14 | Type strain | DSM 20054, ATCC 14869, NCDO 1749, WDCM 00099, CCUG 30670

Strain identifier

BacDive ID: 6655

Type strain:

Species: Lactobacillus brevis

Strain Designation: 14, Bb14

Culture col. no.: DSM 20054, ATCC 14869, NCDO 1749, WDCM 00099, CCUG 30670

Section

Name and taxonomic classification
Morphology and physiology
Culture and growth conditions
Isolation, sampling and environmental information
Application and interaction
Molecular biology
Strain availability

External links

Information on the name and the taxonomic classification.Name and taxonomic classification

[Ref.: #8485]	Domain	Bacteria
[Ref.: #8485]	Phylum	Firmicutes
[Ref.: #8485]	Class	Bacilli
[Ref.: #8485]	Order	Lactobacillales
[Ref.: #8485]	Family	Lactobacillaceae
[Ref.: #8485]	Genus	Lactobacillus
[Ref.: #8485]	Species	Lactobacillus brevis
[Ref.: #8485]	Full Scientific Name	Lactobacillus brevis (Orla-Jensen 1919) Bergey et al. 1934
[Ref.: #8485]	Strain Designation	14, Bb14
[Ref.: #8485]	Type strain

		Prokaryotic Nomenclature Up-to-date (PNU)	Taxonomical status	Literature reference
	Last PNU update	19-11-2019 (DD-MM-YYYY)
[Ref.: #20215]	Domain	Bacteria
[Ref.: #20215]	Phylum	Firmicutes
[Ref.: #20215]	Class	Bacilli		Int. J. Syst. Bacteriol. 38:220
[Ref.: #20215]	Family	Lactobacillaceae		Int. J. Syst. Bacteriol. 30:225
[Ref.: #20215]	Genus	Lactobacillus	genus (AL)	Int. J. Syst. Bacteriol. 30:225
[Ref.: #20215]	Species	Lactobacillus brevis	comb. nov. (AL)	Int. J. Syst. Bacteriol. 30:225
[Ref.: #20215]	Full Scientific Name	Lactobacillus brevis (Orla-Jensen 1919) Bergey et al. 1934

Information on morphological and physiological propertiesMorphology and physiology

[Ref.: #22929]

Gram stain

positive

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #22929]		catalase	-	1.11.1.6

Fatty acid profile

Type of FA analysis

whole cell analysis

Incubation medium

Agar/liquid

Incubation temperature (°C)

Incubation time (d)

Incubation pH

Incubation oxygen condition

Treatment/condition

Software version

Library/peak naming table

System

Instrument

Method/protocol

CCUG

Cutoff value

Reference

[Ref.: #50207]

Fatty acid	Percentage	ECL
14:0	5.4	14
16:0	18.8	16
16:1 ω7c	11.7	15.819
16:1 ω7c/15:0 ISO 2OH	10	15.85
17:0 CYCLO	5.4	16.888
18:1 ω7c /12t/9t	17.3	17.824
18:2 ω6,9c/18:0 ANTE	4.7	17.724
19:0 CYCLO ω8c	20.3	18.9
19:0 CYCLO ω9c	6.4	18.87

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #8485]	4212	-	-	-	-	+	+	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #8485]	4211	-	-	-	-	+	+	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-
[Ref.: #8485]	4210	-	-	-	-	+	+	+	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	+	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+
[Ref.: #8485]	4209	-	-	-	-	+	+	+	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	+	-	-	+	-	-	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	+
[Ref.: #8485]	4208	-	-	-	-	+	+	+	-	-	-	+	+	+	-	-	-	-	-	-	-	-	+/-	+/-	-	-	-	-	-	+	+/-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+/-	-	+/-

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #22929]	D-Adonitol	-	builds acid from
[Ref.: #22929]	D-Arabitol	-	builds acid from
[Ref.: #22929]	D-Fructose	+	builds acid from
[Ref.: #22929]	D-Galactose	+	builds acid from
[Ref.: #22929]	D-Mannitol	-	builds acid from
[Ref.: #22929]	D-Ribose	+	builds acid from
[Ref.: #22929]	D-Xylose	+	builds acid from
[Ref.: #22929]	Esculin	+	builds acid from
[Ref.: #22929]	L-Arabinose	+	builds acid from
[Ref.: #22929]	L-Arabitol	-	builds acid from
[Ref.: #22929]	Lactose	-	hydrolysis
[Ref.: #22929]	Maltose	+	builds acid from
[Ref.: #22929]	Melibiose	+/-	builds acid from
[Ref.: #22929]	Methyl alpha-D-glucopyranoside	+	builds acid from
[Ref.: #22929]	Methyl beta-D-xylopyranoside	-	builds acid from
[Ref.: #22929]	N-Acetyl-D-glucosamine	+/-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

[Ref.: #8485]	Murein short key	A11.31
[Ref.: #8485]	Murein types	A4alpha L-Lys-D-Asp

[Ref.: #50207]

Oxygen tolerance

anaerobe

Information on culture and growth conditionsCulture and growth conditions

[Ref.: #8485]

Culture medium

MRS MEDIUM (DSMZ Medium 11), 30°C, microaerophilic

[Ref.: #8485]

Culture medium growth

[Ref.: #8485]

Culture medium link

medium recipe provided by DSMZ

[Ref.: #41832]

Culture medium

MEDIUM 40- for Lactobacillus and Leuconostoc

[Ref.: #41832]

Culture medium growth

[Ref.: #41832]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #8485]	growth	30 ̊C
[Ref.: #41832]	growth	37 ̊C
[Ref.: #50207]	growth	30 ̊C

[Ref.: #8485]	Temperature range	mesophilic
[Ref.: #41832]	Temperature range	mesophilic
[Ref.: #50207]	Temperature range	mesophilic

Information on isolation source, the sampling and environmental conditionsIsolation, sampling and environmental information

[Ref.: #8485]

Sample type/isolated from

faeces

[Ref.: #50207]

Sample type/isolated from

Feces

Isolation sources categories

#Host Body Product

#Gastrointestinal tract

#Feces (Stool)

What are isolation sources categories?

* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hostsApplication and interaction

[Ref.: #8485]

Biosafety level

Risk group (German classification)

Information on genomic background e.g. entries in nucleic sequence databassMolecular biology

[Ref.: #8485]

GC-content

42.7 mol%

Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence database	Sequence accession description	Sequence accession number	Sequence type	Sequence length (bp)	Associated NCBI tax ID
[Ref.: #8485]	whole genome shotgun sequence, Whole Genome Shotgun (WGS) Sequence INSDC	Lactobacillus brevis ATCC 14869 = DSM 20054, whole genome shotgun sequencing project	AZCP00000000		11	649758
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Lactobacillus brevis malolactic enzyme (mle) gene, partial cds	AF098778		533	649758	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Lactobacillus brevis clone L44 16S ribosomal RNA gene, partial sequence	AF404709	16S	506	649758	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Lactobacillus brevis clone L44 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	AF405353	16S	525	649758	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Lactobacillus brevis clone L44 HSP60 gene, partial cds	AF405388		552	649758	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Lactobacillus brevis partial mRNA for putative elongation factor Tu (tuf gene), strain ATCC 14869	AJ418932		761	649758	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Lactobacillus brevis intergenic spacer and partial 23S rRNA gene, strain ATCC 14869	AJ616223		715	649758	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Lactobacillus brevis surface layer protein SlpB (slpB) gene, complete cds	AY040846		1748	649758	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Lactobacillus brevis surface layer protein SlpC (slpC) gene, complete cds	AY040847		1525	649758	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Lactobacillus brevis surface layer protein SlpD (slpD) gene, complete cds	AY040848		1499	649758	*
[Ref.: #20218]	Marker Gene (DDBJ Direct submission)	Lactobacillus brevis gene for 16S ribosomal RNA, partial sequence	D31681	16S	202	649758	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Lactobacillus brevis strain ATCC 14869 RecA gene, partial cds	DQ080023		453	649758	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Lactobacillus brevis strain ATCC 14869 16S-23S ribosomal RNA intergenic spacer and 23S ribosomal RNA gene, partial sequence	EU161594	16S	767	649758	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Lactobacillus brevis strain ATCC 14869 RecA (recA) gene, partial cds	EU188855		627	649758	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Lactobacillus brevis strain ATCC 14869 16S ribosomal RNA gene, partial sequence	EU194349	16S	1430	649758	*
[Ref.: #20218]	INSDC Sequence	Lactobacillus brevis ATCC 14869 16S ribosomal RNA gene, partial sequence	M58810	16S	1569	649758	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Lactobacillus brevis strain DSM 20054 RpoB protein (rpoB) gene, partial cds	AF531267		169	649758	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Lactobacillus brevis partial 23S rRNA gene, strain DSM20054, 5' end	AJ542558		361	649758	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Lactobacillus brevis radh gene for R-specific alcohol dehydrogenase	AJ544275		759	649758	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Lactobacillus brevis partial gnd gene for 6-phosphogluconate dehydrogenase	AJ582124		1350	649758	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Lactobacillus brevis strain DSM 20054 GroEL (hsp60) gene, partial cds	AY424329		506	649758	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Lactobacillus brevis strain DSM 20054 16S ribosomal RNA gene, partial sequence	EF468097	16S	487	649758	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Lactobacillus brevis ATCC 14869 beta-xylosidase (xynB1) gene, complete cds	KF305639		1683	649758	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Lactobacillus brevis ATCC 14869 arabinofuranosidase (abf3) gene, complete cds	KF305640		981	649758	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Lactobacillus brevis ATCC 14869 beta-xylosidase 2 (xynB2) gene, complete cds	KF305641		1623	649758	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Lactobacillus brevis alpha-L-arabinofuranosidase 1 (abf1) gene, complete cds	HM363023		1491	649758	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Lactobacillus brevis alpha-L-arabinofuranosidase 2 (abf2) gene, complete cds	HM363024		1506	649758	*
[Ref.: #20218]	Marker Gene (DDBJ Direct submission)	Lactobacillus brevis gene for 16S rRNA, partial sequence, strain: JCM 1059	AB289046	16S	663	1580	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Lactobacillus brevis strain JCM 1059 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	AY582720	16S	343	1580	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Lactobacillus brevis pheS gene for phenylalanyl-tRNA synthase alpha subunit, type strain LMG 6906T	AM087680		455	1580	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Lactobacillus brevis rpoA gene for RNA polymerase alpha subunit, type strain LMG 6906T	AM087777		571	1580	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Lactobacillus brevis partial recA gene for recombinase A, strain LMG 7944T	AJ621625		600	1580	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	L.brevis 16S rRNA gene	X61134	16S	1509	649758	*

Availability in culture collectionsStrain availability

[Ref.: #8485]

Culture collection no.

DSM 20054, ATCC 14869, NCDO 1749, WDCM 00099, CCUG 30670

[Ref.: #8485]

Strain history

<- ATCC <- P.A. Hansen, Bb14 <- Roy. Techn. Coll., Copenhagen <- S. Orla-Jensen, 14 (Betabacterium breve)

[Ref.: #20218]

Associated Passport(s) in StrainInfo

13214, 13212, 137342, 866281

References

#8485

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20054

#20215

Leibniz Institute DSMZ D.Gleim, M.Kracht, N.Weiss et. al.: Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since .

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#22929

Thi Phuong Nam Bui, Yeon-Ju Kim, Jun-Gyo In, Deok-Chun Yang: Lactobacillus koreensis sp. nov., isolated from the traditional Korean food kimchi. IJSEM 61: 772 - 776 2011 ( DOI 10.1099/ijs.0.021386-0 , PubMed 20435754 )

#41832 ; Curators of the CIP;
#50207 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 30670
* These References are textmined

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