Levilactobacillus brevis (DSM 6235, CCM 7932, D 13)

Name: Levilactobacillus brevis (DSM 6235, CCM 7932, D 13)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6654

Type strain

Strain Designation D 13

Culture col. no. DSM 6235 CCM 7932 D 13 CIP 103474

NCBI tax ID(s) 1580

Special Collections DSMZ CIP

History DSMZ <- W. Back, D 13. (Lactobacillus frigidus) CIP <- 1992, DSM <- W. Back: strain D 13, Lactobacillus frigidus

Links

Levilactobacillus brevis D 13 is a facultative anaerobe, Gram-positive, rod-shaped bacterium that was isolated from spoilt beer.

Gram-positive rod-shaped facultative anaerobe Bacteria

Name and taxonomic classification

SI-ID 8909

LMG 16322,PCM 2570,CIP 103474,NCIMB 13697,CECT 5172,KACC 10553,DSM 6235

@ref 20215

Last LPSN update 2026-05-29 08:26:27

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Lactobacillaceae

Genus Levilactobacillus

Species Levilactobacillus brevis

Full scientific name Levilactobacillus brevis (Orla-Jensen 1919) Zheng et al. 2020

Synonyms (2)

"Betabacterium breve"
Lactobacillus brevis

BacDive ID	Other strains from **Levilactobacillus brevis** (22)	Type strain
6655	L. brevis 14, Bb14, Bb 14, DSM 20054, ATCC 14869, NCDO ... (type strain)
6650	L. brevis 5 N, DSM 1267, NCIB 8836
6651	L. brevis DSM 1268, NCIB 8561
6652	L. brevis DSM 1269, NCIB 8562
6653	L. brevis No. 2, DSM 2647
6656	L. brevis 269Y, CCUG 21531, DSM 20556, ATCC 8287, CCM ...
139935	L. brevis FAL 25/53, DSM 13201
161016	L. brevis JCM 1065, LMG 12022
161017	L. brevis JCM 1066, NCTC 6107
162006	L. brevis JCM 17312
164748	L. brevis JCM 34252
165586	L. brevis JCM 7731
165597	L. brevis JCM 7753
165601	L. brevis JCM 7757
165602	L. brevis JCM 7758
165610	L. brevis JCM 7775
171318	L. brevis 370, CRBIP24.156
171319	L. brevis 63 AC, CRBIP24.61
171320	L. brevis 158-73, CRBIP24.71
171321	L. brevis CRBIP24.92, Br 23
171322	L. brevis CRBIP24.93, Br 391
175190	L. brevis HUN084, AG48, DSM 115837

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
117043	positive	rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
2551	MRS MEDIUM (DSMZ Medium 11)	Medium recipe at MediaDive	Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
41669	MEDIUM 40- for Lactobacillus and Leuconostoc		Distilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
117043	CIP Medium 72	Medium recipe at CIP
117043	CIP Medium 40	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
2551	positive	growth	30
41669	positive	growth	37
117043	positive	growth	10-37
117043	negative	growth	45

Physiology and metabolism

Oxygen tolerance

117043

Oxygen tolerancefacultative anaerobe

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	-	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	+	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	+	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
117043	17632 ChEBI	nitrate	-	reduction
117043	17632 ChEBI	nitrate	+	respiration
117043	16301 ChEBI	nitrite	-	reduction
68371	0 ChEBI	Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
117043	15688 ChEBI	acetoin	-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
117043	alcohol dehydrogenase	+	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	+	3.2.1.31	from API zym
117043	catalase	-	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
117043	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
117043	ornithine decarboxylase	-	4.1.1.17
117043	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
117043	-	-	+	+	-	+	+	+	-	-	+	+	+	+	+	+	+	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
2551	-	-	-	-	+	+	+	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
2551	-	-	-	-	+	+	+	-	-	-	+	+	+	-	-	-	-	-	-	-	-	+	+	-	-	+	-	-	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+/-	-	-
2551	-	-	-	-	+	+	+/-	-	-	-	+/-	+	+/-	-	-	-	-	-	-	-	-	-	+/-	-	-	+	-	-	+/-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
2551	-	-	-	-	+	+	+	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-
2551	-	-	-	-	+	+	+	-	-	-	+	+	+	-	-	-	-	-	-	-	+	+	-	-	-	+	-	-	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+/-	+/-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Food production	#Fermented
#Environmental	#Microbial community	-
#Engineered	#Food production	#Beverage

Isolation

@ref	Sample type
2551	spoilt beer
117043	Other, Spoilt beer

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
2551	1	Risk group (German classification) According to TRBA 466
117043	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Combined acid hydrolysis and fermentation improves bioactivity of citrus flavonoids in vitro and in vivo.	Konig A, Sadova N, Dornmayr M, Schwarzinger B, Neuhauser C, Stadlbauer V, Wallner M, Woischitzschlager J, Muller A, Tona R, Kofel D, Weghuber J.	Commun Biol	10.1038/s42003-023-05424-7	2023
Enzymology	Inactivation kinetics of beer spoilage bacteria (Lactobacillus brevis, Lactobacillus casei, and Pediococcus damnosus) during acid washing of brewing yeast.	Munford ARG, Chaves RD, Sant'Ana AS	Food Microbiol	10.1016/j.fm.2020.103513	2020
	Correction to: Modeling the inactivation of Lactobacillus brevis DSM 6235 and retaining the viability of brewing pitching yeast submitted to acid and chlorine washing.	Munford ARG, Chaves RD, Granato D, Sant'Ana AS	Appl Microbiol Biotechnol	10.1007/s00253-020-10738-1	2020
Pathogenicity	Modeling the inactivation of Lactobacillus brevis DSM 6235 and retaining the viability of brewing pitching yeast submitted to acid and chlorine washing.	Munford ARG, Chaves RD, Granato D, Sant'Ana AS	Appl Microbiol Biotechnol	10.1007/s00253-020-10534-x	2020

References

#2551	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 6235
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#41669	; Curators of the CIP;
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#117043	Collection of Institut Pasteur ; Curators of the CIP; CIP 103474
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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About us

Levilactobacillus brevis (DSM 6235, CCM 7932, D 13)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Metabolite utilization

Metabolite tests

Enzymes

API zym

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome-based predictions

Literature

Literature

References

BacDive

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