Name: Levilactobacillus brevis D 13
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6654

Type strain:

Species: Levilactobacillus brevis

Strain Designation: D 13

Culture col. no.: DSM 6235, CCM 7932, CIP 103474

Strain history: CIP <- 1992, DSM <- W. Back: strain D 13, Lactobacillus frigidus

NCBI tax ID(s): 1580 (species)

SI-ID 8909

LMG 16322, CIP 103474, NCIMB 13697, CECT 5172, KACC 10553, DSM 6235

Other strains from Levilactobacillus brevis

L. brevis 14, Bb14, Bb 14, DSM 20054, ATCC ... (type strain)
L. brevis 5 N, DSM 1267, NCIB 8836
L. brevis DSM 1268, NCIB 8561
L. brevis DSM 1269, NCIB 8562
L. brevis No. 2, DSM 2647
L. brevis 269Y, CCUG 21531, DSM 20556, ATCC ...
L. brevis FAL 25/53, DSM 13201
L. brevis JCM 1065, LMG 12022
L. brevis JCM 1066, NCTC 6107
L. brevis JCM 17312
L. brevis JCM 34252
L. brevis JCM 7731
L. brevis JCM 7753
L. brevis JCM 7757
L. brevis JCM 7758
L. brevis JCM 7775
L. brevis 370, CRBIP24.156
L. brevis 63 AC, CRBIP24.61
L. brevis 158-73, CRBIP24.71
L. brevis CRBIP24.92, Br 23
L. brevis CRBIP24.93, Br 391

Section

Levilactobacillus brevis D 13 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from spoilt beer.

Gram-positive
rod-shaped
facultative anaerobe
mesophilic
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Levilactobacillus*
	Species	**Levilactobacillus brevis**
	Full Scientific Name (LPSN)	Levilactobacillus brevis (Orla-Jensen 1919) Zheng et al. 2020

	Synonym	Lactobacillus brevis
	Synonym	"Betabacterium* breve"*

Information on morphological and physiological properties Morphology

[Ref.: #117043]	Gram stain	positive

[Ref.: #117043]	Cell shape	rod-shaped

[Ref.: #117043]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #2551]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #2551]

Culture medium growth

[Ref.: #2551]

Culture medium link

Medium recipe at MediaDive

[Ref.: #2551]

Culture medium composition

expand / minimize

[Ref.: #41669]

Culture medium

MEDIUM 40- for Lactobacillus and Leuconostoc

[Ref.: #41669]

Culture medium growth

[Ref.: #41669]

Culture medium composition

expand / minimize

[Ref.: #117043]

Culture medium

CIP Medium 72

[Ref.: #117043]

Culture medium growth

[Ref.: #117043]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #117043]

Culture medium

CIP Medium 40

[Ref.: #117043]

Culture medium growth

[Ref.: #117043]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #2551]		growth	30 °C
[Ref.: #41669]		growth	37 °C
[Ref.: #117043]		growth	10-37 °C
[Ref.: #117043]	no	growth	45 °C

[Ref.: #2551]	Temperature range	mesophilic
[Ref.: #41669]	Temperature range	mesophilic
[Ref.: #117043]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #117043]

Oxygen tolerance

facultative anaerobe

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #117043]	nitrate ChEBI	-	reduction
[Ref.: #117043]	nitrate ChEBI	+	respiration
[Ref.: #117043]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #117043]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #117043]	alcohol dehydrogenase	+	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	+	3.2.1.31	* from API zym
[Ref.: #117043]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #117043]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #117043]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #117043]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #2551]	14250	-	-	-	-	+	+	+	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #2551]	14249	-	-	-	-	+	+	+	-	-	-	+	+	+	-	-	-	-	-	-	-	-	+	+	-	-	+	-	-	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+/-	-	-
[Ref.: #2551]	14248	-	-	-	-	+	+	+/-	-	-	-	+/-	+	+/-	-	-	-	-	-	-	-	-	-	+/-	-	-	+	-	-	+/-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #2551]	14247	-	-	-	-	+	+	+	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-
[Ref.: #2551]	14246	-	-	-	-	+	+	+	-	-	-	+	+	+	-	-	-	-	-	-	-	+	+	-	-	-	+	-	-	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+/-	+/-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #117043]	7549	-	-	+	+	-	+	+	+	-	-	+	+	+	+	+	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #2551]	Sample type/isolated from	spoilt beer

[Ref.: #117043]	Sample type/isolated from	Other, Spoilt beer
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Fermented
#Environmental	#Microbial community	-
#Engineered	#Food production	#Beverage

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #2551]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #117043]	Biosafety level	1	Risk group (French classification)

Availability in culture collections External links

[Ref.: #2551]

Culture collection no.

DSM 6235, CCM 7932, CIP 103474

[Ref.: #76128]

SI-ID 8909

Literature:

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Inactivation kinetics of beer spoilage bacteria (Lactobacillus brevis, Lactobacillus casei, and Pediococcus damnosus) during acid washing of brewing yeast.	Munford ARG, Chaves RD, Sant'Ana AS	Food Microbiol	10.1016/j.fm.2020.103513	2020	*
Pathogenicity	Modeling the inactivation of Lactobacillus brevis DSM 6235 and retaining the viability of brewing pitching yeast submitted to acid and chlorine washing.	Munford ARG, Chaves RD, Granato D, Sant'Ana AS	Appl Microbiol Biotechnol	10.1007/s00253-020-10534-x	2020	*
	Correction to: Modeling the inactivation of Lactobacillus brevis DSM 6235 and retaining the viability of brewing pitching yeast submitted to acid and chlorine washing.	Munford ARG, Chaves RD, Granato D, Sant'Ana AS	Appl Microbiol Biotechnol	10.1007/s00253-020-10738-1	2020	*

References

#2551

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 6235

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#41669 ; Curators of the CIP;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#76128

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID8909.1 )

#117043 Collection of Institut Pasteur ; Curators of the CIP; CIP 103474
* These data were automatically processed and therefore are not curated

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