Lactobacillus kitasatonis (CCUG 53874, CIP 108218, DSM 16761)

Name: Lactobacillus kitasatonis (CCUG 53874, CIP 108218, DSM 16761)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6627

Type strain

Strain Designation Ch-J-2-11, S1-99

Culture col. no. CCUG 53874 CIP 108218 DSM 16761 Ch-J-2-11 JCM 1039 4 more

NCBI tax ID(s) 1423767 237446

Special Collections DSMZ CCUG JCM KCTC CIP

History <- JCM <- T. Mitsuoka, strain Ch-J-2-11 T. Mitsuoka Ch-J-2-11. <- JCM <- IPCR, S1-99 <- T Mitsuoka, Ch-J-2-11 CIP <- 2003, JCM <- T. Mitsuoka: strain Ch-J-2-11

Links

Lactobacillus kitasatonis Ch-J-2-11 is a microaerophile, rod-shaped bacterium that was isolated from chicken, intestine.

rod-shaped microaerophile genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 63137

JCM 1039,KCTC 3155,LMG 22685,CIP 108218,LMG 23133,DSM 16761,CCUG 53874,BCRC 17387,CCRC 17387,KCTC 315,BCRC 17512,CCRC 17512

@ref 20215

Last LPSN update 2026-02-09 11:19:16

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Lactobacillaceae

Genus Lactobacillus

Species Lactobacillus kitasatonis

Full scientific name Lactobacillus kitasatonis Mukai et al. 2003

BacDive ID	Other strains from **Lactobacillus kitasatonis** (2)	Type strain
160577	L. kitasatonis Cla-CZ-130, DSM 109052
160943	L. kitasatonis JCM 1038, KCTC 3154, LMG 23132

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
67771		rod-shaped
67771	positive
120450	positive	rod-shaped
125438			91
125438	positive		92.953

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
6496	MRS MEDIUM (DSMZ Medium 11)	Medium recipe at MediaDive	Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
38264	MEDIUM 40- for Lactobacillus and Leuconostoc		Distilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
120450	CIP Medium 40	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
6496	positive	growth	37
38264	positive	growth	37
60044	positive	growth	37
67770	positive	growth	37
67771	positive	growth	37
120450	positive	growth	30-45
120450	negative	growth	10
120450	negative	growth	15
120450	negative	growth	22

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
6496	microaerophile
60044	microaerophile
67771	microaerophile
125439	obligate aerobe	97

Spore formation

@ref	Spore formation	Confidence
67771
125438		91.892

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
120450	15824 ChEBI	D-fructose	-	degradation
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
120450	17634 ChEBI	D-glucose	-	degradation
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
120450	16024 ChEBI	D-mannose	-	degradation
68371	16988 ChEBI	D-ribose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
120450	17716 ChEBI	lactose	-	degradation
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
120450	17306 ChEBI	maltose	-	degradation
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
120450	17632 ChEBI	nitrate	-	reduction
120450	17632 ChEBI	nitrate	+	respiration
120450	16301 ChEBI	nitrite	-	reduction
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
120450	17992 ChEBI	sucrose	-	degradation
68371	17992 ChEBI	sucrose	+	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Antibiotic resistance

@ref	Metabolite	Is sensitive	Is resistant
120450	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
120450	15688 ChEBI	acetoin	-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
120450	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
120450	catalase	-	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	-		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
120450	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
120450	ornithine decarboxylase	-	4.1.1.17
120450	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
120450	-	-	+	-	-	+	-	+	-	-	+	+	+	-	-	+	+	+	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
6496	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	+	-	-	+	-	+/-	+	-	-	+	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-
6496	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	+	-	-	+	-	+	+	-	-	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-
120450	not determinedn.d.	-	-	-	-	-	-	-	-	-	+	+	+	+/-	-	-	-	-	-	-	-	-	+	+/-	+/-	+/-	+/-	+	+	-	-	+	-	-	-	-	+/-	-	-	+	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Birds	#Chicken
#Host Body-Site	#Gastrointestinal tract	#Stomach

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
6496	chicken, intestine	Japan	JPN	Asia
60044	Chicken intestine	Japan	JPN	Asia
67770	Chicken intestine
67771	From chicken intestine	Japan	JPN	Asia
120450	Chicken intestine	Japan	JPN	Asia

Taxonmaps

69479

Global distribution of 16S sequence LC064885 (>99% sequence identity) for Lactobacillus from Microbeatlas

Aquatic Samples	67
Soil Samples	44
Animal Samples	5968
Plant Samples	80
Total Samples	6159

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6496	1	Risk group (German classification) According to TRBA 466
120450	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM143443v1 assembly for Lactobacillus kitasatonis DSM 16761 = JCM 1039	scaffold	GCA_001434435	1423767.3	2660238082	1423767	68.61
67771	ASM61528v1 assembly for Lactobacillus kitasatonis DSM 16761 = JCM 1039	contig	GCA_000615285	1236941.3	2565956592	1423767	65.84

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Lactobacillus kitasatonis gene for 16S rRNA, partial sequence, strain: JCM 1039	AB289190	648	237446
6496	Lactobacillus kitasatonis gene for 16S ribosomal RNA, partial sequence	AB107638	1506	237446
67770	Lactobacillus kitasatonis gene for 16S ribosomal RNA, partial sequence, strain: JCM 1039	LC064885	1464	237446
124043	Lactobacillus kitasatonis partial 16S rRNA gene, type strain DSM 16761T	FR683090	1445	237446
124043	Lactobacillus kitasatonis strain JCM 1039 16S ribosomal RNA gene, partial sequence.	MN587975	980	237446

GC content

@ref	GC-content (mol%)	Method
6496	37
67771	37.5	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Lactobacillus kitasatonis DSM 16761 = JCM 1039
NCBI: GCA_001434435

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	97.00	no
125439	gram_stain	BacteriaNetⓘ	positive	69.10	no
125439	motility	BacteriaNetⓘ	yes	54.60	no
125439	spore_formation	BacteriaNetⓘ	yes	57.80	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Lactobacillus kitasatonis DSM 16761 = JCM 1039
PATRIC: 1423767.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	92.95	yes
125438	anaerobic	anaerobicⓘ	no	79.95	yes
125438	spore-forming	spore-formingⓘ	no	91.89	no
125438	aerobic	aerobicⓘ	no	97.71	yes
125438	thermophilic	thermophileⓘ	no	95.00	yes
125438	flagellated	motile2+ⓘ	no	91.00	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
	Susceptibility of Lactobacillaceae Strains to Aminoglycoside Antibiotics in the Light of EFSA Guidelines.	Dec M, Herman-Ostrzyzek K, Zomer A, Urban-Chmiel R.	Life (Basel)	10.3390/life15050732	2025
	The S-layer Associated Serine Protease Homolog PrtX Impacts Cell Surface-Mediated Microbe-Host Interactions of Lactobacillus acidophilus NCFM.	Johnson BR, O'Flaherty S, Goh YJ, Carroll I, Barrangou R, Klaenhammer TR.	Front Microbiol	10.3389/fmicb.2017.01185	2017
Metabolism	Functional Analysis of an S-Layer-Associated Fibronectin-Binding Protein in Lactobacillus acidophilus NCFM.	Hymes JP, Johnson BR, Barrangou R, Klaenhammer TR.	Appl Environ Microbiol	10.1128/aem.00024-16	2016
	Restriction of growth and biofilm formation of ESKAPE pathogens by caprine gut-derived probiotic bacteria.	Saini P, Ayyanna R, Kumar R, Bhowmick SK, Bhaskar V, Dey B.	Front Microbiol	10.3389/fmicb.2024.1428808	2024
	Microbiota-driven antitumour immunity mediated by dendritic cell migration.	Lin NY, Fukuoka S, Koyama S, Motooka D, Tourlousse DM, Shigeno Y, Matsumoto Y, Yamano H, Murotomi K, Tamaki H, Irie T, Sugiyama E, Kumagai S, Itahashi K, Tanegashima T, Fujimaki K, Ito S, Shindo M, Tsuji T, Wake H, Watanabe K, Maeda Y, Enokida T, Tahara M, Yamashita R, Fujisawa T, Nomura M, Kawazoe A, Goto K, Doi T, Shitara K, Mano H, Sekiguchi Y, Nakamura S, Benno Y, Nishikawa H.	Nature	10.1038/s41586-025-09249-8	2025
Phylogeny	Lactobacillus curtus sp. nov., isolated from beer in Finland.	Asakawa Y, Takesue N, Asano S, Shimotsu S, Iijima K, Suzuki K, Motoyama Y, Aizawa M.	Int J Syst Evol Microbiol	10.1099/ijsem.0.002220	2017
Phylogeny	Paenibacillus tarimensis sp. nov., isolated from sand in Xinjiang, China.	Wang M, Yang M, Zhou G, Luo X, Zhang L, Tang Y, Fang C.	Int J Syst Evol Microbiol	10.1099/ijs.0.65445-0	2008
Phylogeny	Lactobacillus kitasatonis sp. nov., from chicken intestine.	Mukai T, Arihara K, Ikeda A, Nomura K, Suzuki F, Ohori H	Int J Syst Evol Microbiol	10.1099/ijs.0.02815-0	2003

References

#6496	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16761
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#38264	; Curators of the CIP;
#60044	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 53874
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771	Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#120450	Collection of Institut Pasteur ; Curators of the CIP; CIP 108218
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Lactobacillus kitasatonis (CCUG 53874, CIP 108218, DSM 16761)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Antibiotic resistance

Metabolite tests

Enzymes

API zym

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_001434435

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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