Liquorilactobacillus satsumensis (DSM 16230, NRIC 0604, CCUG 52399)

Name: Liquorilactobacillus satsumensis (DSM 16230, NRIC 0604, CCUG 52399)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6621

Type strain

Culture col. no. DSM 16230 NRIC 0604 CCUG 52399 JCM 12392 NCIMB 14549 1 more

NCBI tax ID(s) 259059

Special Collections DSMZ CCUG JCM CIP Literature strains

History DSMZ <- A. Endo <- NRIC JCM <-- NRIC 0604 <-- A. Endo. CIP <- 2008, JCM <- A.Endo, Agricult. Univ. Tokyo, Japan <- NRIC, Sochu mash: strain 12392

Links

Liquorilactobacillus satsumensis DSM 16230 is a microaerophile, Gram-positive, motile bacterium that was isolated from shochu mash.

Gram-positive motile rod-shaped microaerophile genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 130993

LMG 22973,DSM 16230,JCM 12392,CCUG 52399,CIP 109882

@ref 20215

Last LPSN update 2026-05-29 08:26:27

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Lactobacillaceae

Genus Liquorilactobacillus

Species Liquorilactobacillus satsumensis

Full scientific name Liquorilactobacillus satsumensis (Endo and Okada 2005) Zheng et al. 2020

Synonyms (1)

Lactobacillus satsumensis

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
31301	positive	1.25 µm	0.7 µm	rod-shaped
122494	positive			rod-shaped

Colony morphology

59539

Incubation period3 days

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
6344	MRS MEDIUM (DSMZ Medium 11)	Medium recipe at MediaDive	Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
37743	MEDIUM 40- for Lactobacillus and Leuconostoc		Distilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
122494	CIP Medium 40	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
6344	positive	growth	30
31301	positive	growth	15-45
31301	positive	optimum	30
37743	positive	growth	37
59539	positive	growth	30-37
67770	positive	growth	30
122494	positive	growth	15-45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
6344	microaerophile
31301	facultative anaerobe
59539	anaerobe
59539	microaerophile
122494	facultative anaerobe
125439	facultative anaerobe	94.257

Halophily

@ref	Salt	Growth	Tested relation	Concentration
31301	NaCl	positive	growth	<5 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	+	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	+	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
31301	28757 ChEBI	fructose	+	carbon source
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
31301	28260 ChEBI	galactose	+	carbon source
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
31301	17234 ChEBI	glucose	+	carbon source
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	+	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	-	builds acid from	from API 50CH acid
31301	29864 ChEBI	mannitol	+	carbon source
31301	37684 ChEBI	mannose	+	carbon source
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
122494	17632 ChEBI	nitrate	-	reduction
122494	17632 ChEBI	nitrate	+	respiration
122494	16301 ChEBI	nitrite	-	reduction
68371	0 ChEBI	Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371	0 ChEBI	Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
31301	17814 ChEBI	salicin	+	carbon source
68371	17814 ChEBI	salicin	+	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
31301	17992 ChEBI	sucrose	+	carbon source
68371	17992 ChEBI	sucrose	+	builds acid from	from API 50CH acid
31301	27082 ChEBI	trehalose	+	carbon source
68371	27082 ChEBI	trehalose	+	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	+	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
122494	15688 ChEBI	acetoin	+

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
122494	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
122494	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	-		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
122494	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
122494	ornithine decarboxylase	-	4.1.1.17
122494	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
122494	-	+	+	-	-	+	-	-	-	-	+	+	-	-	-	+	+	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
6344	-	-	-	-	-	-	-	-	-	-	-	+	+	+	+	-	-	-	+	-	-	-	+	-	+	+	+	-	-	-	-	+	+	-	-	-	-	-	-	-	+	-	+	-	-	-	-	-	-	-
122494	not determinedn.d.	-	-	-	-	-	-	-	-	-	+/-	+	+	+/-	+	+/-	-	-	+	-	-	+/-	+/-	+/-	-	+	+	-	-	-	-	+	+	-	-	-	-	-	-	+/-	+	-	+	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Food production	#Fermented
#Engineered	#Food production	#Beverage

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
6344	shochu mash	Miyazaki pref.	Japan	JPN	Asia
59539	Shochu mash		Japan	JPN	Asia
67770	Mashes of shouchu (traditional Japanese distilled spirits)
122494	Food, Fermented rice					2005

Taxonmaps

69479

Global distribution of 16S sequence LC311746 (>99% sequence identity) for Liquorilactobacillus satsumensis from Microbeatlas

Aquatic Samples	70
Soil Samples	88
Animal Samples	3288
Plant Samples	120
Total Samples	3566

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6344	1	Risk group (German classification) According to TRBA 466
122494	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM143519v1 assembly for Liquorilactobacillus satsumensis DSM 16230 = JCM 12392	scaffold	GCA_001435195	1423801.4	2671180250	1423801	53.72
67770	ASM131204v1 assembly for Liquorilactobacillus satsumensis DSM 16230 = JCM 12392	contig	GCA_001312045	1423801.3	2728369706	1423801	0

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
6344	Lactobacillus satsumensis gene for 16S rRNA, partial sequence, strain:NRIC 0604	AB154519	1556	259059
67770	Lactobacillus satsumensis gene for 16S ribosomal RNA, partial sequence, strain: JCM 12392	LC311746	1497	259059
124043	Lactobacillus satsumensis gene for 16S rRNA, partial sequence, strain: JCM 12392.	AB289300	652	259059

GC content

@ref	GC-content (mol%)	Method
6344	40.2
31301	40
67770	39.9	genome sequence analysis
67770	40	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM143519v1 assembly for Liquorilactobacillus satsumensis DSM 16230 = JCM 12392
BV-BRC: 1423801.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	94.26	no
125439	gram_stain	BacteriaNetⓘ	variable	55.57	no
125439	motility	BacteriaNetⓘ	yes	54.49	no
125439	spore_formation	BacteriaNetⓘ	yes	46.78	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Lactobacillus satsumensis DSM 16230 = JCM 12392
PATRIC: 1423801.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	89.66	yes
125438	anaerobic	anaerobicⓘ	no	85.97	no
125438	spore-forming	spore-formingⓘ	no	73.32	no
125438	aerobic	aerobicⓘ	no	94.27	yes
125438	thermophilic	thermophileⓘ	no	95.90	yes
125438	flagellated	motile2+ⓘ	yes	57.96	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Comprehensive structural characterization of water-soluble and water-insoluble homoexopolysaccharides from seven lactic acid bacteria	Ernst L, Offermann H, Werner A, Wefers D.	Carbohydrate polymers.		2024
	Comprehensive structural characterization of water-soluble and water-insoluble homoexopolysaccharides from seven lactic acid bacteria.	Ernst L, Offermann H, Werner A, Wefers D.	Carbohydr Polym	10.1016/j.carbpol.2023.121417	2024
	Adhesive Interactions Between Lactic Acid Bacteria and beta-Lactoglobulin: Specificity and Impact on Bacterial Location in Whey Protein Isolate.	Gomand F, Borges F, Guerin J, El-Kirat-Chatel S, Francius G, Dumas D, Burgain J, Gaiani C.	Front Microbiol	10.3389/fmicb.2019.01512	2019
	Liquorilactobacillus satsumensis from water kefir yields alpha-glucan polysaccharides with prebiotic and synbiotic qualities	Tan LL, Ngiam JJ, Sim ESZ, Conway PL, Loo SCJ.	Carbohydrate polymers.		2022
	Liquorilactobacillus satsumensis from water kefir yields alpha-glucan polysaccharides with prebiotic and synbiotic qualities.	Tan LL, Ngiam JJ, Sim ESZ, Conway PL, Loo SCJ.	Carbohydr Polym	10.1016/j.carbpol.2022.119515	2022
	Quality and shelf life assessment of a new beverage produced from water kefir grains and red pitaya	Bueno RS, Ressutte JB, Hata NNY, Henrique-Bana FC, Guergoletto KB, de Oliveira AG, Spinosa WA.	Lebensm Wiss Technol	10.1016/j.lwt.2020.110770	2021
	Exploration adhesion properties of Liquorilactobacillus and Lentilactobacillus isolated from two different sources of tepache kefir grains.	Oviedo-Leon JF, Cornejo-Mazon M, Ortiz-Hernandez R, Torres-Ramirez N, Hernandez-Sanchez H, Castro-Rodriguez DC.	PLoS One	10.1371/journal.pone.0297900	2024
	Microbial Diversity and Volatile Flavor Changes during Gayangju Fermentation, a Traditional Korean House Rice Wine.	Song YR, Lim BU, Baik SH.	Foods	10.3390/foods11172604	2022
	Identification of Key Parameters Inducing Microbial Modulation during Backslopped Kombucha Fermentation.	Daval C, Tran T, Verdier F, Martin A, Alexandre H, Grandvalet C, Tourdot-Marechal R.	Foods	10.3390/foods13081181	2024
Biotechnology	Characterization of strictly lytic phages infecting Oenococcus oeni from Merlot wines and proposal of a new genus.	Barchi Y, Oviedo-Hernandez F, Chaib A, Philippe C, Claisse O, Cambillau C, Goulet A, Marrec CL.	Microbiol Spectr	10.1128/spectrum.02588-24	2025
	Potential Probiotic Strains From Milk and Water Kefir Grains in Singapore-Use for Defense Against Enteric Bacterial Pathogens.	Tan LL, Tan CH, Ng NKJ, Tan YH, Conway PL, Loo SCJ.	Front Microbiol	10.3389/fmicb.2022.857720	2022
	The core microbiomes and associated metabolic potential of water kefir as revealed by pan multi-omics.	Breselge S, Skibinska I, Yin X, Brennan L, Kilcawley K, Cotter PD.	Commun Biol	10.1038/s42003-025-07808-3	2025
	Exploration of Lactiplantibacillus fabifermentans and Furfurilactobacillus rossiae as potential cocoa fermentation starters.	Korcari D, Ricci G, Fanton A, Emide D, Barbiroli A, Fortina MG.	J Appl Microbiol	10.1111/jam.15687	2022
	Characterization and insight mechanism of an acid-adapted beta-Glucosidase from Lactobacillus paracasei and its application in bioconversion of glycosides.	Xie Y, Yan X, Li C, Wang S, Jia L.	Front Bioeng Biotechnol	10.3389/fbioe.2024.1334695	2024
	Unveiling the microbiome during post-partum uterine infection: a deep shotgun sequencing approach to characterize the dairy cow uterine microbiome.	Basbas C, Garzon A, Schlesener C, van Heule M, Profeta R, Weimer BC, Silva-Del-Rio N, Byrne BA, Karle B, Aly SS, Lima FS, Pereira RV.	Anim Microbiome	10.1186/s42523-023-00281-5	2023
	Microbial communities of a variety of 75 homemade fermented vegetables.	Thierry A, Madec MN, Chuat V, Bage AS, Picard O, Grondin C, Rue O, Mariadassou M, Marche L, Valence F.	Front Microbiol	10.3389/fmicb.2023.1323424	2023
Phylogeny	Bacterial Populations in International Artisanal Kefirs.	Sindi A, Badsha MB, Unlu G.	Microorganisms	10.3390/microorganisms8091318	2020
Phylogeny	Lactobacillus sucicola sp. nov., a motile lactic acid bacterium isolated from oak tree (Quercus sp.) sap.	Irisawa T, Okada S	Int J Syst Evol Microbiol	10.1099/ijs.0.006478-0	2009
Phylogeny	Lactobacillus aquaticus sp. nov., isolated from a Korean freshwater pond.	Manes-Lazaro R, Song J, Pardo I, Cho JC, Ferrer S	Int J Syst Evol Microbiol	10.1099/ijs.0.008276-0	2009
Phylogeny	Lactobacillus oeni sp. nov., from wine.	Manes-Lazaro R, Ferrer S, Rossello-Mora R, Pardo I	Int J Syst Evol Microbiol	10.1099/ijs.0.007567-0	2009
Phylogeny	Lactobacillus ceti sp. nov., isolated from beaked whales (Ziphius cavirostris).	Vela AI, Fernandez A, Espinosa de los Monteros A, Goyache J, Herraez P, Tames B, Cruz F, Dominguez L, Fernandez-Garayzabal JF	Int J Syst Evol Microbiol	10.1099/ijs.0.65372-0	2008
Phylogeny	Lactobacillus satsumensis sp. nov., isolated from mashes of shochu, a traditional Japanese distilled spirit made from fermented rice and other starchy materials.	Endo A, Okada S	Int J Syst Evol Microbiol	10.1099/ijs.0.63248-0	2005

References

#6344	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16230
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#27619	IJSEM 83 2005 ( DOI 10.1099/ijs.0.63248-0 , PubMed 15653857 )
#31301	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27619
#37743	; Curators of the CIP;
#59539	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 52399
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#122494	Collection of Institut Pasteur ; Curators of the CIP; CIP 109882
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Liquorilactobacillus satsumensis (DSM 16230, NRIC 0604, CCUG 52399)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Halophily

Metabolite utilization

Metabolite tests

Enzymes

API zym

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_001435195

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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