Name: Latilactobacillus sakei subsp. carnosus R60
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6603

Type strain:

Species: Latilactobacillus sakei subsp. carnosus

Strain Designation: R60

Culture col. no.: CIP 109872, DSM 15740, CCUG 41580

Strain history: CIP <- 2008, CCUG, Lactobacillus curvatus melibiosus <- 1998, G. Klein, FUB, Berlin, Germany: strain 41580

NCBI tax ID(s): 214325 (subspecies)

Other strains from Latilactobacillus sakei subsp. carnosus

L. sakei subsp. carnosus R 14b/a, DSM 15831, CCUG 31331, ... (type strain)

Section

Latilactobacillus sakei subsp. carnosus R60 is a microaerophile, mesophilic, Gram-positive bacterium of the family Lactobacillaceae.

Gram-positive
rod-shaped
mesophilic
microaerophile
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Latilactobacillus*
	Species	**Latilactobacillus sakei subsp. carnosus**
	Full Scientific Name (LPSN)	Latilactobacillus sakei subsp. carnosus (Torriani et al. 1996) Zheng et al. 2020

	Synonym	Lactobacillus sake subsp. carnosus
	Synonym	Lactobacillus curvatus subsp. melibiosus
	Synonym	Lactobacillus sakei subsp. carnosus

Information on morphological and physiological properties Morphology

[Ref.: #122354]	Gram stain	positive

[Ref.: #122354]	Cell shape	rod-shaped

[Ref.: #122354]	Motility	no

[Ref.: #55131]

Incubation period

1 day

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6092]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #6092]

Culture medium growth

[Ref.: #6092]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6092]

Culture medium composition

expand / minimize

[Ref.: #41041]

Culture medium

MEDIUM 40- for Lactobacillus and Leuconostoc

[Ref.: #41041]

Culture medium growth

[Ref.: #41041]

Culture medium composition

expand / minimize

	Kind of temperature		Temperature
[Ref.: #6092]		growth	30 °C
[Ref.: #41041]		growth	37 °C
[Ref.: #122354]		growth	15-37 °C
[Ref.: #122354]	no	growth	45 °C

[Ref.: #6092]	Temperature range	mesophilic
[Ref.: #41041]	Temperature range	mesophilic
[Ref.: #122354]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #6092]	Oxygen tolerance	microaerophile
[Ref.: #55131]	Oxygen tolerance	microaerophile
[Ref.: #122354]	Oxygen tolerance	facultative anaerobe

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #122354]	nitrate ChEBI	-	reduction
[Ref.: #122354]	nitrate ChEBI	+	respiration
[Ref.: #122354]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #122354]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #122354]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #122354]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #122354]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #122354]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #122354]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #55131]

Fatty acid	Percentage	ECL
C16:0	32.4	16
C18:1 ω7c /12t/9t	16.2	17.824
C18:2 ω6,9c/C18:0 ANTE	15.4	17.724
C18:0	13.5	18
C18:1 ω9c	13	17.769
C16:1 ω7c	4.4	15.819
C19:0 CYCLO ω9c	2.8	18.87
C14:0	2.2	14

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #122354]	11677	not determinedn.d.	-	-	-	-	+/-	-	-	-	-	+/-	+/-	+/-	+/-	-	-	-	-	-	-	-	-	+/-	-	-	-	-	+/-	-	-	+/-	+/-	+/-	-	-	-	-	-	-	+/-	-	-	-	-	-	-	-	+/-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #122354]	12179	-	+	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6092]	Country	Germany
[Ref.: #6092]	Country ISO 3 Code	DEU
[Ref.: #6092]	Continent	Europe

[Ref.: #122354]	Country	Italy
[Ref.: #122354]	Country ISO 3 Code	ITA
[Ref.: #122354]	Continent	Europe
[Ref.: #122354]	Isolation date	1996
* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6092]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #122354]	Biosafety level	1	Risk group (French classification)

Availability in culture collections External links

[Ref.: #6092]

Culture collection no.

CIP 109872, DSM 15740, CCUG 41580

References

#6092

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15740

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#41041 ; Curators of the CIP;
#55131 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 41580

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#122354 Collection of Institut Pasteur ; Curators of the CIP; CIP 109872
* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Latilactobacillus sakei subsp. carnosus in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF