Secundilactobacillus paracollinoides (DSM 15502, JCM 11969, LA2)

Name: Secundilactobacillus paracollinoides (DSM 15502, JCM 11969, LA2)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6596

Type strain

Strain Designation LA2

Culture col. no. DSM 15502 JCM 11969 LA2 VTT E-042481 LMG 22473 2 more

NCBI tax ID(s) 240427

Special Collections DSMZ JCM CIP Literature strains

History DSMZ <- K. Suzuki; LA2 <- N. Yako JCM <-- K. Suzuki LA2. CIP <- 2004, JCM <- 2003, K. Suzuki, Japan: strain LA2

Links

Secundilactobacillus paracollinoides LA2 is a facultative anaerobe, Gram-positive bacterium that was isolated from brewery environment.

Gram-positive facultative anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 130842

DSM 15502,JCM 11969,LMG 22473,CIP 108394,VTT E-042481,CECT 8644

@ref 20215

Last LPSN update 2026-05-29 08:26:26

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Lactobacillaceae

Genus Secundilactobacillus

Species Secundilactobacillus paracollinoides

Full scientific name Secundilactobacillus paracollinoides (Suzuki et al. 2004) Zheng et al. 2020

Synonyms (1)

Lactobacillus paracollinoides

BacDive ID	Other strains from **Secundilactobacillus paracollinoides** (5)	Type strain
6597	S. paracollinoides DSM 20197, ATCC 8291, NCIB 11719, NCTC 4955, ...
161625	S. paracollinoides JCM 15728
161626	S. paracollinoides JCM 15729
161627	S. paracollinoides JCM 15730
161628	S. paracollinoides JCM 15731

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
22931	positive
121550	positive	rod-shaped
125438	positive		92.279

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
5963	MRS MEDIUM (DSMZ Medium 11)	Medium recipe at MediaDive	Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
34957	MEDIUM 40- for Lactobacillus and Leuconostoc		Distilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
121550	CIP Medium 40	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
5963	positive	growth	25
34957	positive	growth	25
67770	positive	growth	25
121550	positive	growth	15-30
121550	negative	growth	10
121550	negative	growth	37
121550	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
121550	facultative anaerobe
125439	obligate aerobe	93.203

Spore formation

@ref	Spore formation	Confidence
22931
125439		97.293

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
22931	16808 ChEBI	2-dehydro-D-gluconate	-	builds acid from
22932	16808 ChEBI	2-dehydro-D-gluconate	-	builds acid from
22931	58143 ChEBI	5-dehydro-D-gluconate	-	builds acid from
22932	58143 ChEBI	5-dehydro-D-gluconate	-	builds acid from
22931	27613 ChEBI	amygdalin	-	builds acid from
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
22931	18305 ChEBI	arbutin	-	builds acid from
22932	18305 ChEBI	arbutin	-	builds acid from
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
22932	17057 ChEBI	cellobiose	-	builds acid from
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
22931	17108 ChEBI	D-arabinose	-	builds acid from
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
22931	18333 ChEBI	D-arabitol	-	builds acid from
22932	18333 ChEBI	D-arabitol	-	builds acid from
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
22931	15824 ChEBI	D-fructose	-	builds acid from
22932	15824 ChEBI	D-fructose	-	builds acid from
68371	15824 ChEBI	D-fructose	-	builds acid from	from API 50CH acid
22931	28847 ChEBI	D-fucose	-	builds acid from
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
22931	17634 ChEBI	D-glucose	+	builds acid from
22931	62318 ChEBI	D-lyxose	-	builds acid from
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
22932	16899 ChEBI	D-mannitol	-	builds acid from
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
22931	16024 ChEBI	D-mannose	-	builds acid from
22932	16024 ChEBI	D-mannose	-	builds acid from
68371	16024 ChEBI	D-mannose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
22931	17317 ChEBI	D-sorbose	-	builds acid from
22931	16443 ChEBI	D-tagatose	-	builds acid from
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
22931	65327 ChEBI	D-xylose	+	builds acid from
22931	17113 ChEBI	erythritol	-	builds acid from
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
22932	4853 ChEBI	esculin	+	builds acid from
68371	4853 ChEBI	esculin	-	builds acid from	from API 50CH acid
22931	16813 ChEBI	galactitol	-	builds acid from
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
22931	28066 ChEBI	gentiobiose	-	builds acid from
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
22931	24265 ChEBI	gluconate	-	builds acid from
22932	24265 ChEBI	gluconate	-	builds acid from
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
22931	17754 ChEBI	glycerol	-	builds acid from
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
22931	28087 ChEBI	glycogen	-	builds acid from
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
22931	15443 ChEBI	inulin	-	builds acid from
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
22931	30849 ChEBI	L-arabinose	+/-	builds acid from
22931	18403 ChEBI	L-arabitol	-	builds acid from
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
22931	18287 ChEBI	L-fucose	-	builds acid from
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
22931	65328 ChEBI	L-xylose	-	builds acid from
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
22931	17716 ChEBI	lactose	-	builds acid from
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
22931	17306 ChEBI	maltose	+	builds acid from
22931	29864 ChEBI	mannitol	-	builds acid from
22931	6731 ChEBI	melezitose	-	builds acid from
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
22931	28053 ChEBI	melibiose	+	builds acid from
22932	28053 ChEBI	melibiose	+	builds acid from
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
22931	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
22931	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from
22932	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
22931	37657 ChEBI	methyl D-glucoside	-	builds acid from
22931	17268 ChEBI	myo-inositol	-	builds acid from
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
22931	506227 ChEBI	N-acetyl-D-glucosamine	-	builds acid from
22932	506227 ChEBI	N-acetyl-D-glucosamine	-	builds acid from
68371	59640 ChEBI	N-acetylglucosamine	-	builds acid from	from API 50CH acid
121550	17632 ChEBI	nitrate	-	reduction
121550	16301 ChEBI	nitrite	-	reduction
68371	0 ChEBI	Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371	0 ChEBI	Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
22931	16634 ChEBI	raffinose	-	builds acid from
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
22931	26546 ChEBI	rhamnose	-	builds acid from
22931	15963 ChEBI	ribitol	-	builds acid from
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
22931	33942 ChEBI	ribose	+	builds acid from
22931	17814 ChEBI	salicin	-	builds acid from
22932	17814 ChEBI	salicin	-	builds acid from
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
22931	30911 ChEBI	sorbitol	-	builds acid from
22931	28017 ChEBI	starch	-	builds acid from
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
22931	17992 ChEBI	sucrose	-	builds acid from
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
22931	27082 ChEBI	trehalose	-	builds acid from
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
22931	17151 ChEBI	xylitol	-	builds acid from
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
121550	15688 ChEBI	acetoin	-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
121550	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
22932	alpha-galactosidase	+	3.2.1.22
68382	alpha-galactosidase	+	3.2.1.22	from API zym
22932	alpha-glucosidase	+	3.2.1.20
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
22932	beta-glucosidase	+	3.2.1.21
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
22931	catalase	-	1.11.1.6
121550	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	-		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
121550	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
121550	ornithine decarboxylase	-	4.1.1.17
121550	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
121550	-	-	+	-	-	+	+	-	-	-	-	+	+	+	-	+	+	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
5963	-	-	-	-	+	+	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
5963	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
121550	not determinedn.d.	-	-	-	+/-	+/-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Engineered	#Food production

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
5963	brewery environment	Japan	JPN	Asia
67770	Brewery environment
121550	Beer	Japan	JPN	Asia

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
5963	1	Risk group (German classification) According to TRBA 466
121550	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM143511v1 assembly for Secundilactobacillus paracollinoides DSM 15502 = JCM 11969	scaffold	GCA_001435115	1423785.4	2671180263	1423785	22.98
67770	ASM131113v1 assembly for Secundilactobacillus paracollinoides DSM 15502 = JCM 11969	contig	GCA_001311135	1423785.3	2728369671	1423785	18.5

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Lactobacillus paracollinoides gene for 16S-23S rRNA intergenic spacer, partial sequence, 300 bp fragment, strain: DSM 15502	AB117956	296	240427
20218	Lactobacillus paracollinoides gene for 16S-23S rRNA intergenic spacer, partial sequence, 500 bp fragment, strain: DSM 15502	AB117958	519	240427
20218	Lactobacillus paracollinoides partial 16S rRNA gene, type strain DSM 15502T	AJ786665	1531	240427
20218	Lactobacillus paracollinoides gene for 16S rRNA, partial sequence, strain: JCM 11969	AB289231	608	240427
20218	Lactobacillus paracollinoides gene for 16S ribosomal RNA, partial sequence, strain: JCM 11969	AB370878	1489	240427
20218	Lactobacillus paracollinoides gene for 16S ribosomal RNA, partial sequence, strain: JCM 11969	AB473623	1525	240427
20218	Lactobacillus sp. AB No.74 16S rRNA gene	E16651	1524	1591
67770	Lactobacillus paracollinoides gene for 16S ribosomal RNA, partial sequence	LC483558	1510	240427

GC content

@ref	GC-content (mol%)	Method
5963	44.8
67770	46.8	genome sequence analysis
67770	44.8	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM143511v1 assembly for Secundilactobacillus paracollinoides DSM 15502 = JCM 11969
BV-BRC: 1423785.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	93.20	no
125439	gram_stain	BacteriaNetⓘ	positive	68.46	no
125439	motility	BacteriaNetⓘ	no	74.79	no
125439	spore_formation	BacteriaNetⓘ	no	97.29	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Lactobacillus paracollinoides DSM 15502 = JCM 11969
PATRIC: 1423785.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	92.28	yes
125438	anaerobic	anaerobicⓘ	no	82.39	yes
125438	spore-forming	spore-formingⓘ	no	78.49	yes
125438	aerobic	aerobicⓘ	no	94.78	yes
125438	thermophilic	thermophileⓘ	no	95.99	no
125438	flagellated	motile2+ⓘ	no	87.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	A Novel Glutamyl (Aspartyl)-Specific Aminopeptidase A from Lactobacillus delbrueckii with Promising Properties for Application.	Stressler T, Ewert J, Merz M, Funk J, Claassen W, Lutz-Wahl S, Schmidt H, Kuhn A, Fischer L.	PLoS One	10.1371/journal.pone.0152139	2016
Phylogeny	Taxonomic note "Lactobacillus pastorianus" (Van Laer, 1892) a former synonym for Lactobacillus paracollinoides.	Ehrmann MA, Vogel RF	Syst Appl Microbiol	10.1016/j.syapm.2004.09.007	2005
Phylogeny	Longispora urticae sp. nov., isolated from rhizosphere soil of Urtica urens L., and emended descriptions of the species Longisporaalbida and Longisporafulva.	Piao C, Jin L, Zhao J, Liu C, Zhao Y, Wang X, Xiang W.	Int J Syst Evol Microbiol	10.1099/ijsem.0.002288	2017
Phylogeny	Lactobacillus silagei sp. nov., isolated from orchardgrass silage.	Tohno M, Kitahara M, Irisawa T, Masuda T, Uegaki R, Ohkuma M, Tajima K	Int J Syst Evol Microbiol	10.1099/ijs.0.053124-0	2013
Phylogeny	Lactobacillus paracollinoides sp. nov., isolated from brewery environments.	Suzuki K, Funahashi W, Koyanagi M, Yamashita H	Int J Syst Evol Microbiol	10.1099/ijs.0.02722-0	2004

References

#5963	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15502
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#22931	Koji Suzuki, Wataru Funahashi, Masahiro Koyanagi, Hiroshi Yamashita: Lactobacillus paracollinoides sp. nov., isolated from brewery environments. IJSEM 54: 115 - 117 2004 ( DOI 10.1099/ijs.0.02722-0 , PubMed 14742467 )
#22932	Masanori Tohno, Maki Kitahara, Tomohiro Irisawa, Takaharu Masuda, Ryuichi Uegaki, Moriya Ohkuma, Kiyoshi Tajima: Lactobacillus silagei sp. nov., isolated from orchardgrass silage. IJSEM 63: 4613 - 4618 2013 ( DOI 10.1099/ijs.0.053124-0 , PubMed 23919960 )
#34957	; Curators of the CIP;
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#121550	Collection of Institut Pasteur ; Curators of the CIP; CIP 108394
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Secundilactobacillus paracollinoides (DSM 15502, JCM 11969, LA2)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Metabolite tests

Enzymes

API zym

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_001435115

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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