Levilactobacillus spicheri (DSM 15429, LMG 21871, LTH 5753)

Name: Levilactobacillus spicheri (DSM 15429, LMG 21871, LTH 5753)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6595

Type strain

Culture col. no. DSM 15429 LMG 21871 LTH 5753 NBRC 107155 CIP 108581 2 more

NCBI tax ID(s) 1423805 216463

Special Collections DSMZ JCM CIP

History <- C. B. Meroth, Univ. Hohenheim 2004, C. Hertel, Hohenheim Univ., Stuttgart, Germany: strain LTH 5753 LMG 21871 <-- DSM 15429 <-- C. Meroth LTH 5753. CIP <- 2004, C. Hertel, Hohenheim Univ., Stuttgart, Germany: strain LTH 5753

Links

Levilactobacillus spicheri DSM 15429 is a microaerophile, mesophilic, Gram-positive prokaryote that was isolated from rice sourdough.

Gram-positive rod-shaped microaerophile mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 127684

NBRC:107155,LMG 21871,DSM 15429,JCM 15956,CIP 108581

@ref 20215

Last LPSN update 2025-12-16 09:48:22

Domain Bacillati

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Lactobacillaceae

Genus Levilactobacillus

Species Levilactobacillus spicheri

Full scientific name Levilactobacillus spicheri (Meroth et al. 2004) Zheng et al. 2020

Synonyms (1)

Lactobacillus spicheri

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility	Confidence
123260	positive	rod-shaped
125438	positive			94.29

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
5817	MRS MEDIUM (DSMZ Medium 11)	Medium recipe at MediaDive	Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
40026	MEDIUM 40- for Lactobacillus and Leuconostoc		Distilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
123260	CIP Medium 40	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
5817	positive	growth	30	mesophilic
40026	positive	growth	30	mesophilic
67770	positive	growth	28	mesophilic
123260	positive	growth	15-37
123260	negative	growth	10
123260	negative	growth	45

Physiology and metabolism

Oxygen tolerance

5817

Oxygen tolerancemicroaerophile

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	+	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	-	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	+	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	+	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	+	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	+	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	+	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	+	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
123260	17632 ChEBI	nitrate	-	reduction
123260	17632 ChEBI	nitrate	-	respiration
123260	16301 ChEBI	nitrite	-	reduction
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	+	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Antibiotic resistance

@ref	Metabolite	Is sensitive	Is resistant
123260	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
123260	15688 ChEBI	acetoin	-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
123260	alcohol dehydrogenase	+	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
123260	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	-		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
123260	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
123260	ornithine decarboxylase	-	4.1.1.17
123260	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
123260	-	-	-	-	-	+	-	-	-	-	+	+	+	+	-	+	+	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
5817	-	-	-	-	+	+	+	-	-	+	+	+	+	-	-	-	-	-	-	-	-	+/-	+/-	-	-	+	-	-	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	+
123260	not determinedn.d.	-	-	-	+	+	+	-	-	+	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Food production	-
#Host	#Plants	#Herbaceous plants (Grass,Crops)

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
5817	rice sourdough	Germany	DEU	Europe
67770	Rice sourdough
123260	Food, Rice sourdough	Germany	DEU	Europe

Taxonmaps

69479

Global distribution of 16S sequence LC480813 (>99% sequence identity) for Levilactobacillus spicheri subclade from Microbeatlas

Aquatic Samples	91
Soil Samples	55
Animal Samples	666
Plant Samples	104
Total Samples	916

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
5817	1	Risk group (German classification) According to TRBA 466
123260	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM143509v1 assembly for Levilactobacillus spicheri DSM 15429	scaffold	GCA_001435095	1423805.4	2700989146	1423805	66.71
67770	ASM799195v1 assembly for Levilactobacillus spicheri NBRC 107155	contig	GCA_007991955	216463.4		216463	56.28

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
5817	Lactobacillus spicheri 16S rRNA gene, type strain LTH 5753T	AJ534844	1536	216463
67770	Lactobacillus spicheri JCM 15956 gene for 16S ribosomal RNA, partial sequence	LC480813	1504	216463
124043	Lactobacillus spicheri gene for 16S rRNA, partial sequence, strain: NBRC 107155.	AB626069	1491	216463
124043	Lactobacillus spicheri strain CIP 108581 16S-23S ribosomal RNA intergenic spacer and 23S ribosomal RNA gene, partial sequence.	EU161580	802	216463

GC content

@ref	GC-content (mol%)	Method
5817	55.5
67770	55.9	genome sequence analysis
67770	55	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Levilactobacillus spicheri DSM 15429
NCBI: GCA_001435095

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	63.70	no
125439	motility	BacteriaNetⓘ	yes	50.80	no
125439	gram_stain	BacteriaNetⓘ	variable	71.50	no
125439	oxygen_tolerance	BacteriaNetⓘ	microaerophile	86.60	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Lactobacillus spicheri DSM 15429
PATRIC: 1423805.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	94.29	no
125438	anaerobic	anaerobicⓘ	no	84.45	yes
125438	spore-forming	spore-formingⓘ	no	80.32	no
125438	aerobic	aerobicⓘ	no	94.23	no
125438	thermophilic	thermophileⓘ	no	94.50	no
125438	flagellated	motile2+ⓘ	no	87.63	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Coarse-grained model of serial dilution dynamics in synthetic human gut microbiome.	Mahajan T, Maslov S.	PLoS Comput Biol	10.1371/journal.pcbi.1013222	2025
Metabolism	Display of fungi xylanase on Escherichia coli cell surface and use of the enzyme in xylan biodegradation.	Qu W, Xue Y, Ding Q.	Curr Microbiol	10.1007/s00284-015-0781-2	2015
	Safety and efficacy of a feed additive consisting of Lentilactobacillus diolivorans (formerly Lactobacillus diolivorans) DSM 33625 as a silage additive for all animal species (Lactosan GmbH & Co.KG).	EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP), Bampidis V, Azimonti G, de Lourdes Bastos M, Christensen H, Dusemund B, Fasmon Durjava M, Kouba M, Lopez-Alonso M, Lopez Puente S, Marcon F, Mayo B, Pechova A, Petkova M, Ramos F, Sanz Y, Villa RE, Woutersen R, Anguita M, Brozzi R, Ortuno J, Garcia-Cazorla Y.	EFSA J	10.2903/j.efsa.2023.7820	2023
	Draft Genome Sequence of Bifidobacterium lemurum DSM 28807^T Isolated from the Gastrointestinal Tracts of Ring-Tailed Lemurs (Lemur catta).	Toh H, Matsubara T, Tomida S, Mimura I, Arakawa K, Kikusui T, Morita H.	Genome Announc	10.1128/genomea.01656-16	2017
	Substrate recognition mode of a glycoside hydrolase family 42 beta-galactosidase from Bifidobacterium longum subspecies infantis (BiBga42A) revealed by crystallographic and mutational analyses.	Gotoh A, Hidaka M, Sakurama H, Nishimoto M, Kitaoka M, Sakanaka M, Fushinobu S, Katayama T.	Microbiome Res Rep	10.20517/mrr.2023.14	2023
	Phenotypic and Safety Assessment of the Cheese Strain Lactiplantibacillus plantarum LL441, and Sequence Analysis of its Complete Genome and Plasmidome.	Florez AB, Vazquez L, Rodriguez J, Mayo B.	Int J Mol Sci	10.3390/ijms24010605	2022
	High-level expression of the xylanase from Thermomyces lanuginosus in Escherichia coli	Yin E, Le Y, Pei J, Shao W, Yang Q.	World J Microbiol Biotechnol	10.1007/s11274-007-9469-5	2008
Metabolism	Evaluation of nitric oxide production by lactobacilli.	Xu J, Verstraete W.	Appl Microbiol Biotechnol	10.1007/s002530100616	2001
Genetics	Tracking the Taxonomy of the Genus Bifidobacterium Based on a Phylogenomic Approach.	Lugli GA, Milani C, Duranti S, Mancabelli L, Mangifesta M, Turroni F, Viappiani A, van Sinderen D, Ventura M.	Appl Environ Microbiol	10.1128/aem.02249-17	2018
Genetics	Comparative genomic and phylogenomic analyses of the Bifidobacteriaceae family.	Lugli GA, Milani C, Turroni F, Duranti S, Mancabelli L, Mangifesta M, Ferrario C, Modesto M, Mattarelli P, Jiri K, van Sinderen D, Ventura M.	BMC Genomics	10.1186/s12864-017-3955-4	2017
	Application of GFAT as a novel selection marker to mediate gene expression.	Wu G, Sun Y, Qu W, Huang Y, Lu L, Li L, Shao W.	PLoS One	10.1371/journal.pone.0017082	2011
	Effect of Rice Flour Fermentation with Lactobacillus spicheri DSM 15429 on the Nutritional Features of Gluten-Free Muffins.	Chis MS, Paucean A, Man SM, Bonta V, Pop A, Stan L, Beldean Tatar BV, Pop CR, Muresan V, Muste S	Foods	10.3390/foods9060822	2020
	Textural and Sensory Features Changes of Gluten Free Muffins Based on Rice Sourdough Fermented with Lactobacillus spicheri DSM 15429.	Chis MS, Paucean A, Man SM, Muresan V, Socaci SA, Pop A, Stan L, Rusu B, Muste S	Foods	10.3390/foods9030363	2020
Phylogeny	Nonomuraea mangrovi sp. nov., an actinomycete isolated from mangrove soil.	Huang H, Liu M, Zhong W, Mo K, Zhu J, Zou X, Hu Y, Bao S.	Int J Syst Evol Microbiol	10.1099/ijsem.0.002954	2018
Phylogeny	Nonomuraea rhizophila sp. nov., an actinomycete isolated from rhizosphere soil.	Zhao GZ, Li J, Huang HY, Zhu WY, Xu LH, Li WJ.	Int J Syst Evol Microbiol	10.1099/ijs.0.028050-0	2011

References

#5817	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15429
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#40026	; Curators of the CIP;
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#123260	Collection of Institut Pasteur ; Curators of the CIP; CIP 108581
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Levilactobacillus spicheri (DSM 15429, LMG 21871, LTH 5753)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Metabolite utilization

Antibiotic resistance

Metabolite tests

Enzymes

API zym

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_001435095

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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