Name: Levilactobacillus spicheri DSM 15429
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6595

Type strain:

Species: Levilactobacillus spicheri

Culture col. no.: DSM 15429, LMG 21871, LTH 5753, JCM 15956, BCRC 80775, CIP 108581, NBRC 107155

Strain history: CIP <- 2004, C. Hertel, Hohenheim Univ., Stuttgart, Germany: strain LTH 5753

NCBI tax ID(s): 1423805 (strain), 216463 (species)

SI-ID 127684

LMG 21871, DSM 15429, JCM 15956, CIP 108581

Section

Levilactobacillus spicheri DSM 15429 is a microaerophile, mesophilic bacterium that was isolated from rice sourdough.

mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Levilactobacillus*
	Species	**Levilactobacillus spicheri**
	Full Scientific Name (LPSN)	Levilactobacillus spicheri (Meroth et al. 2004) Zheng et al. 2020

Synonym

Lactobacillus spicheri

Information on morphological and physiological properties Morphology

[Ref.: #123260]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #123260]	Cell shape	rod-shaped

[Ref.: #123260]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %97.439

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5817]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #5817]

Culture medium growth

[Ref.: #5817]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5817]

Culture medium composition

expand / minimize

[Ref.: #40026]

Culture medium

MEDIUM 40- for Lactobacillus and Leuconostoc

[Ref.: #40026]

Culture medium growth

[Ref.: #40026]

Culture medium composition

expand / minimize

[Ref.: #123260]

Culture medium

CIP Medium 40

[Ref.: #123260]

Culture medium growth

[Ref.: #123260]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #5817]		growth	30 °C
[Ref.: #40026]		growth	30 °C
[Ref.: #67770]		growth	28 °C
[Ref.: #123260]		growth	15-37 °C
[Ref.: #123260]	no	growth	10 °C
[Ref.: #123260]	no	growth	45 °C

[Ref.: #5817]	Temperature range	mesophilic
[Ref.: #40026]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #123260]	Temperature range	psychrophilic
[Ref.: #123260]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #5817]

Oxygen tolerance

microaerophile

[Ref.: #69481]	Ability of spore formation	no Confidence in %93
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.999

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #123260]	nitrate ChEBI	-	reduction
[Ref.: #123260]	nitrate ChEBI	-	respiration
[Ref.: #123260]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #123260]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #123260]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #123260]	alcohol dehydrogenase	+	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #123260]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #123260]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #123260]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #123260]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #5817]	14321	-	-	-	-	+	+	+	-	-	+	+	+	+	-	-	-	-	-	-	-	-	+/-	+/-	-	-	+	-	-	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	+
[Ref.: #123260]	11329	not determinedn.d.	-	-	-	+	+	+	-	-	+	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #123260]	11302	-	-	-	-	-	+	-	-	-	-	+	+	+	+	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5817]	Sample type/isolated from	rice sourdough
[Ref.: #5817]	Country	Germany
[Ref.: #5817]	Country ISO 3 Code	DEU
[Ref.: #5817]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Rice sourdough

[Ref.: #123260]	Sample type/isolated from	Food, Rice sourdough
[Ref.: #123260]	Country	Germany
[Ref.: #123260]	Country ISO 3 Code	DEU
[Ref.: #123260]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	-
#Host	#Plants	#Herbaceous plants (Grass,Crops)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC480813 (>99% sequence identity) for Levilactobacillus spicheri subclade from Microbeatlas

Aquatic Samples	91
Soil Samples	55
Animal Samples	666
Plant Samples	104
Total Samples	916

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5817]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #123260]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5817]	Lactobacillus spicheri 16S rRNA gene, type strain LTH 5753T	AJ534844	1536	ENA	216463 tax ID
[Ref.: #67770]	Lactobacillus spicheri JCM 15956 gene for 16S ribosomal RNA, partial sequence	LC480813	1504	ENA	216463 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Levilactobacillus spicheri DSM 15429	GCA_001435095	scaffold	GenBank	1423805 tax ID
[Ref.: #67770]	Levilactobacillus spicheri NBRC 107155	GCA_007991955	contig	GenBank	216463 tax ID
[Ref.: #66792]	Lactobacillus spicheri DSM 15429	1423805.4	wgs	PATRIC	1423805 tax ID	*
[Ref.: #66792]	Lactobacillus spicheri strain NBRC 107155	216463.4	wgs	PATRIC	216463 tax ID	*
[Ref.: #66792]	Levilactobacillus spicheri DSM 15429	2700989146	draft	JGI IMG/M	1423805 tax ID	*

[Ref.: #5817]	GC-content	55.5 mol%
[Ref.: #67770]	GC-content	55.9 mol%	genome sequence analysis
[Ref.: #67770]	GC-content	55 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Lactobacillus spicheri strain NBRC 107155 PATRIC: 216463.4
[Ref.: #69481]	spore-forming	no	93	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Lactobacillus spicheri DSM 15429 PATRIC: 1423805.4
[Ref.: #69480]	motile	no	93.774	no
[Ref.: #69480]	gram-positive	yes	97.95	no
[Ref.: #69480]	anaerobic	no	89.604	yes
[Ref.: #69480]	halophile	yes	91.352	no
[Ref.: #69480]	spore-forming	no	93.54	no
[Ref.: #69480]	thermophile	no	99.445	yes
[Ref.: #69480]	glucose-util	yes	91.745	no
[Ref.: #69480]	aerobic	no	96.4	no
[Ref.: #69480]	flagellated	no	97.786	no
[Ref.: #69480]	glucose-ferment	yes	82.852	no

Availability in culture collections External links

[Ref.: #5817]

Culture collection no.

DSM 15429, LMG 21871, LTH 5753, JCM 15956, BCRC 80775, CIP 108581, NBRC 107155

[Ref.: #76069]

SI-ID 127684

Literature:

	Title	Authors	Journal	DOI	Year
	Effect of Rice Flour Fermentation with Lactobacillus spicheri DSM 15429 on the Nutritional Features of Gluten-Free Muffins.	Chis MS, Paucean A, Man SM, Bonta V, Pop A, Stan L, Beldean Tatar BV, Pop CR, Muresan V, Muste S	Foods	10.3390/foods9060822	2020	*
	Textural and Sensory Features Changes of Gluten Free Muffins Based on Rice Sourdough Fermented with Lactobacillus spicheri DSM 15429.	Chis MS, Paucean A, Man SM, Muresan V, Socaci SA, Pop A, Stan L, Rusu B, Muste S	Foods	10.3390/foods9030363	2020	*

References

#5817

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15429

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#40026 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#76069

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID127684.1 )

#123260 Collection of Institut Pasteur ; Curators of the CIP; CIP 108581
* These data were automatically processed and therefore are not curated

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Lactobacillus spicheri strain NBRC 107155

Lactobacillus spicheri DSM 15429

Change proposal

Change proposal