Name: Lentilactobacillus diolivorans JKD6
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 6591

Type strain:

Species: Lentilactobacillus diolivorans

Strain Designation: JKD6

Culture col. no.: DSM 14421, LMG 19667, JCM 12183, CIP 107499, KCTC 13927, NBRC 107869

Strain history: CIP <- 2002, DSMZ <- LMG <- M. Vancanneyt <- J. Krooneman: strain JKD6

NCBI tax ID(s): 1423739 (strain), 179838 (species)

SI-ID 14634

LMG 19667, DSM 14421, KCTC 13927, JCM 12183, CIP 107499

Special Collections

Section

When using BacDive for research please cite our paper

Lentilactobacillus diolivorans JKD6 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from maize silage.

Gram-positive
rod-shaped
facultative anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Lentilactobacillus*
	Species	**Lentilactobacillus diolivorans**
	Full Scientific Name (LPSN)	Lentilactobacillus diolivorans (Krooneman et al. 2002) Zheng et al. 2020

Synonym

Lactobacillus diolivorans

Information on morphological and physiological properties Morphology

[Ref.: #118939]	Gram stain	positive
[Ref.: #125438]	Gram stain	positive Confidence in %92.82

[Ref.: #118939]	Cell shape	rod-shaped

[Ref.: #118939]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5345]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #5345]

Culture medium growth

[Ref.: #5345]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5345]

Culture medium composition

expand / minimize

[Ref.: #33809]

Culture medium

MEDIUM 40- for Lactobacillus and Leuconostoc

[Ref.: #33809]

Culture medium growth

[Ref.: #33809]

Culture medium composition

expand / minimize

[Ref.: #118939]

Culture medium

CIP Medium 40

[Ref.: #118939]

Culture medium growth

[Ref.: #118939]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #5345]		growth	30 °C
[Ref.: #33809]		growth	37 °C
[Ref.: #67770]		growth	30 °C
[Ref.: #118939]		growth	22-37 °C
[Ref.: #118939]	no	growth	10 °C
[Ref.: #118939]	no	growth	15 °C
[Ref.: #118939]	no	growth	45 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #118939]	Oxygen tolerance	facultative anaerobe
[Ref.: #125439]	Oxygen tolerance	obligate aerobe Confidence in %98.7

[Ref.: #125439]

Ability of spore formation

no Confidence in %92.1

[Ref.: #5345]	Murein short key	A11.31
[Ref.: #5345]	Murein types	A4alpha L-Lys-D-Asp

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #118939]	nitrate ChEBI	-	reduction
[Ref.: #118939]	nitrate ChEBI	+	respiration
[Ref.: #118939]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #118939]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #118939]	alcohol dehydrogenase	+	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	+	3.2.1.31	* from API zym
[Ref.: #118939]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #118939]	lysine decarboxylase	+	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #118939]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #118939]	oxidase	-
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #5345]	15117	-	-	-	-	+	+	+	-	-	+	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-
[Ref.: #5345]	15116	-	-	-	-	+	+	+	-	-	+	+	+	+	-	-	-	-	-	-	-	-	+/-	-	-	-	+	-	-	+	-	+	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	+/-	-	+/-	-	+/-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #118939]	10399	-	+	+	+	-	+	+	+	+	-	+	+	+	+	+	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5345]	Sample type/isolated from	maize silage
[Ref.: #5345]	Country	Netherlands
[Ref.: #5345]	Country ISO 3 Code	NLD
[Ref.: #5345]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Maize silage
[Ref.: #67770]	Country	Netherlands
[Ref.: #67770]	Country ISO 3 Code	NLD
[Ref.: #67770]	Continent	Europe

[Ref.: #118939]	Sample type/isolated from	Maize silage
[Ref.: #118939]	Country	Netherlands
[Ref.: #118939]	Country ISO 3 Code	NLD
[Ref.: #118939]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered	#Agriculture	-
#Host	#Plants	#Herbaceous plants (Grass,Crops)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC311745 (>99% sequence identity) for Lactobacillaceae from Microbeatlas

Aquatic Samples	383
Soil Samples	317
Animal Samples	4598
Plant Samples	344
Total Samples	5642

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5345]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #118939]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Lactobacillus diolivorans gene for 16S ribosomal RNA, partial sequence, strain: JCM 12183	LC311745	1511	GenBank ENA	179838 tax ID
[Ref.: #5345]	Lactobacillus sp. JKD6 16S ribosomal RNA gene, complete sequence	AF264701	1491	GenBank ENA	179838 tax ID
[Ref.: #124043]	Lactobacillus diolivorans gene for 16S rRNA, partial sequence, strain: JCM 12183.	AB289097	671	GenBank ENA	179838 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Lentilactobacillus diolivorans DSM 14421	GCA_001434255	scaffold	GenBank ENA	1423739 tax ID
[Ref.: #67770]	Lentilactobacillus diolivorans NBRC 107869	GCA_007992335	contig	GenBank ENA	179838 tax ID
[Ref.: #66792]	Lactobacillus diolivorans DSM 14421	1423739.3	wgs	PATRIC	1423739 tax ID	*
[Ref.: #66792]	Lactobacillus diolivorans strain NBRC 107869	179838.8	wgs	PATRIC	179838 tax ID	*
[Ref.: #66792]	Lentilactobacillus diolivorans DSM 14421	2657245078	draft	JGI IMG/M	1423739 tax ID	*

[Ref.: #5345]	GC-content	39.6 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67770]	GC-content	40 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Model	Trait	Prediction	Confidence in %	In training data
	Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Lactobacillus diolivorans DSM 14421 PATRIC: 1423739.3
[Ref.: #125438]	gram-positive	gram-positiveⓘ	yes	92.82	no
[Ref.: #125438]	anaerobic	anaerobicⓘ	no	83.368	yes
[Ref.: #125438]	spore-forming	spore-formingⓘ	no	80.956	no
[Ref.: #125438]	aerobic	aerobicⓘ	no	91.457	yes
[Ref.: #125438]	thermophile	thermophilicⓘ	no	95.002	yes
[Ref.: #125438]	motile2+	flagellatedⓘ	no	88.5	no

	Model	Trait	Prediction	Confidence in %
	Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Lentilactobacillus diolivorans DSM 14421 NCBI: GCA_001434255
[Ref.: #125439]	BacteriaNet	spore_formationⓘ	no	92.1
[Ref.: #125439]	BacteriaNet	motilityⓘ	no	61.9
[Ref.: #125439]	BacteriaNet	gram_stainⓘ	positive	75.6
[Ref.: #125439]	BacteriaNet	oxygen_toleranceⓘ	obligate aerobe	98.7

Availability in culture collections External links

[Ref.: #5345]

Culture collection no.

DSM 14421, LMG 19667, JCM 12183, CIP 107499, KCTC 13927, NBRC 107869

[Ref.: #76065]

SI-ID 14634

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Lactobacillus curieae sp. nov., isolated from stinky tofu brine.	Lei X, Sun G, Xie J, Wei D	Int J Syst Evol Microbiol	10.1099/ijs.0.041830-0	2012	*
Phylogeny	Lactobacillus diolivorans sp. nov., a 1,2-propanediol-degrading bacterium isolated from aerobically stable maize silage.	Krooneman J, Faber F, Alderkamp AC, Elferink SJHWO, Driehuis F, Cleenwerck I, Swings J, Gottschal JC, Vancanneyt M	Int J Syst Evol Microbiol	10.1099/00207713-52-2-639	2002	*

References

#5345

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 14421

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#33809 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#76065

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID14634.1 )

#118939 Collection of Institut Pasteur ; Curators of the CIP; CIP 107499

#124043

Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .

#125438

Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )

#125439

Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Lentilactobacillus diolivorans in external resources:
SILVA
BRENDA
PANGAEA
GBIF

Lactobacillus diolivorans DSM 14421

Lentilactobacillus diolivorans DSM 14421

Change proposal

Change proposal