Lentilactobacillus diolivorans (DSM 14421, LMG 19667, JKD6)

Name: Lentilactobacillus diolivorans (DSM 14421, LMG 19667, JKD6)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6591

Type strain

Strain Designation JKD6

Culture col. no. DSM 14421 LMG 19667 JKD6 NBRC 107869 KCTC 13927 2 more

NCBI tax ID(s) 1423739 179838

Special Collections DSMZ JCM KCTC CIP

History DSMZ <- LMG <- M. Vancanneyt <- J. Krooneman; JKD6 JCM <-- LMG 19667 <-- M. Vancanneyt R-6806 <-- J. Krooneman JKD6. CIP <- 2002, DSMZ <- LMG <- M. Vancanneyt <- J. Krooneman: strain JKD6

Links

Lentilactobacillus diolivorans JKD6 is a facultative anaerobe, Gram-positive, rod-shaped bacterium that was isolated from maize silage.

Gram-positive rod-shaped facultative anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 14634

LMG 19667,DSM 14421,NBRC:107869,KCTC 13927,JCM 12183,CIP 107499

@ref 20215

Last LPSN update 2026-05-29 08:26:26

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Lactobacillaceae

Genus Lentilactobacillus

Species Lentilactobacillus diolivorans

Full scientific name Lentilactobacillus diolivorans (Krooneman et al. 2002) Zheng et al. 2020

Synonyms (1)

Lactobacillus diolivorans

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility	Confidence
118939	positive	rod-shaped
125438	positive			92.82

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
5345	MRS MEDIUM (DSMZ Medium 11)	Medium recipe at MediaDive	Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
33809	MEDIUM 40- for Lactobacillus and Leuconostoc		Distilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
118939	CIP Medium 40	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
5345	positive	growth	30
33809	positive	growth	37
67770	positive	growth	30
118939	positive	growth	22-37
118939	negative	growth	10
118939	negative	growth	15
118939	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
118939	facultative anaerobe
125439	obligate aerobe	99.056

Spore formation

@ref	Spore formation	Confidence
125439		97.995

Murein

@ref	Murein short key	Type
5345	A11.31	A4alpha L-Lys-D-Asp

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	+	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	-	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	+	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	+	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	+	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	+	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	+	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	-	builds acid from	from API 50CH acid
118939	17632 ChEBI	nitrate	-	reduction
118939	17632 ChEBI	nitrate	+	respiration
118939	16301 ChEBI	nitrite	-	reduction
68371	0 ChEBI	Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
118939	15688 ChEBI	acetoin	-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
118939	alcohol dehydrogenase	+	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	+	3.2.1.31	from API zym
118939	catalase	-	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
118939	lysine decarboxylase	+	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
118939	ornithine decarboxylase	-	4.1.1.17
118939	oxidase	-
68382	trypsin	+	3.4.21.4	from API zym
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
118939	-	+	+	+	-	+	+	+	+	-	+	+	+	+	+	+	+	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
5345	-	-	-	-	+	+	+	-	-	+	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-
5345	-	-	-	-	+	+	+	-	-	+	+	+	+	-	-	-	-	-	-	-	-	+/-	-	-	-	+	-	-	+	-	+	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	+/-	-	+/-	-	+/-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Agriculture	-
#Host	#Plants	#Herbaceous plants (Grass,Crops)

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
5345	maize silage	Netherlands	NLD	Europe
67770	Maize silage	Netherlands	NLD	Europe
118939	Maize silage	Netherlands	NLD	Europe

Taxonmaps

69479

Global distribution of 16S sequence LC311745 (>99% sequence identity) for Lactobacillaceae from Microbeatlas

Aquatic Samples	383
Soil Samples	317
Animal Samples	4598
Plant Samples	344
Total Samples	5642

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
5345	1	Risk group (German classification) According to TRBA 466
118939	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM143425v1 assembly for Lentilactobacillus diolivorans DSM 14421	scaffold	GCA_001434255	1423739.3	2657245078	1423739	53.57
67770	ASM799233v1 assembly for Lentilactobacillus diolivorans NBRC 107869	contig	GCA_007992335	179838.8		179838	44.02

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
5345	Lactobacillus sp. JKD6 16S ribosomal RNA gene, complete sequence	AF264701	1491	179838
67770	Lactobacillus diolivorans gene for 16S ribosomal RNA, partial sequence, strain: JCM 12183	LC311745	1511	179838
124043	Lactobacillus diolivorans gene for 16S rRNA, partial sequence, strain: JCM 12183.	AB289097	671	179838

GC content

@ref	GC-content (mol%)	Method
5345	39.6	high performance liquid chromatography (HPLC)
67770	40	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM143425v1 assembly for Lentilactobacillus diolivorans DSM 14421
BV-BRC: 1423739.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.06	no
125439	gram_stain	BacteriaNetⓘ	positive	79.34	no
125439	motility	BacteriaNetⓘ	no	81.05	no
125439	spore_formation	BacteriaNetⓘ	no	98.00	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Lactobacillus diolivorans DSM 14421
PATRIC: 1423739.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	92.82	no
125438	anaerobic	anaerobicⓘ	no	83.37	yes
125438	spore-forming	spore-formingⓘ	no	80.96	no
125438	aerobic	aerobicⓘ	no	91.46	yes
125438	thermophilic	thermophileⓘ	no	95.00	yes
125438	flagellated	motile2+ⓘ	no	88.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Isolation and characterization of Lentilactobacillus diolivorans: a high n-propanol-producing microorganism from Baijiu brewing.	Zhang E, Gao R, Zhu K, Liu P, Liao W, Yang T, Liang X, Yang M, Ming Y, Miao L.	Front Microbiol	10.3389/fmicb.2025.1624097	2025
	Optimization of 1,3-propanediol production from fermentation of crude glycerol by immobilized Bacillus pumilus.	Pinyaphong P, La-Up A.	Heliyon	10.1016/j.heliyon.2024.e35349	2024
Transcriptome	Bacterial Tolerance to 1-Butanol and 2-Butanol: Quantitative Assessment and Transcriptomic Response.	Arsov A, Petrova P, Gerginova M, Tsigoriyna L, Armenova N, Ignatova I, Petrov K.	Int J Mol Sci	10.3390/ijms252413336	2024
	Bacterial Degradation of Antinutrients in Foods: The Genomic Insight.	Arsov A, Tsigoriyna L, Batovska D, Armenova N, Mu W, Zhang W, Petrov K, Petrova P.	Foods	10.3390/foods13152408	2024
Metabolism	Enhancement of 1,3-propanediol production from industrial by-product by Lactobacillus reuteri CH53.	Ju JH, Wang D, Heo SY, Kim MS, Seo JW, Kim YM, Kim DH, Kang SA, Kim CH, Oh BR.	Microb Cell Fact	10.1186/s12934-019-1275-x	2020
Phylogeny	Lactobacillus curieae sp. nov., isolated from stinky tofu brine.	Lei X, Sun G, Xie J, Wei D	Int J Syst Evol Microbiol	10.1099/ijs.0.041830-0	2012
Phylogeny	Lactobacillus diolivorans sp. nov., a 1,2-propanediol-degrading bacterium isolated from aerobically stable maize silage.	Krooneman J, Faber F, Alderkamp AC, Elferink SJHWO, Driehuis F, Cleenwerck I, Swings J, Gottschal JC, Vancanneyt M	Int J Syst Evol Microbiol	10.1099/00207713-52-2-639	2002

References

#5345	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 14421
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#33809	; Curators of the CIP;
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#118939	Collection of Institut Pasteur ; Curators of the CIP; CIP 107499
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Lentilactobacillus diolivorans (DSM 14421, LMG 19667, JKD6)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Murein

Metabolite utilization

Metabolite tests

Enzymes

API zym

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_001434255

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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