Name: Companilactobacillus bobalius 203
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6579

Type strain:

Species: Companilactobacillus bobalius

Strain Designation: 203, Lb 203

Culture col. no.: DSM 19674, CECT 7310, JCM 16180, CIP 109926, KACC 16343, LMG 25239, NBRC 107149

Strain history: CIP <- 2008, CECT, Lactobacillus bobalius <- R. Manes <- A.M. Rodas, Valencia Univ., Valencia, Spain: strain 203

NCBI tax ID(s): 1423788 (strain), 2801451 (species)

SI-ID 360273

CECT 7310, DSM 19674, CIP 109926, LMG 25239, JCM 16180

Section

Companilactobacillus bobalius 203 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from Spanish Bobal grape must.

Gram-positive
rod-shaped
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Companilactobacillus*
	Species	**Companilactobacillus bobalius**
	Full Scientific Name (LPSN)	Companilactobacillus bobalius (Mañes-Lázaro et al. 2008) Zheng et al. 2020

Synonym

Lactobacillus bobalius

Information on morphological and physiological properties Morphology

[Ref.: #32562]	Gram stain	positive
[Ref.: #121566]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #32562]	Cell length	1.65-3.41 µm

[Ref.: #32562]	Cell width	0.71-1.03 µm

[Ref.: #32562]	Cell shape	rod-shaped
[Ref.: #121566]	Cell shape	rod-shaped

[Ref.: #32562]	Motility	no
[Ref.: #121566]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %95.879

[Ref.: #32562]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8248]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #8248]

Culture medium growth

[Ref.: #8248]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8248]

Culture medium composition

expand / minimize

[Ref.: #33686]

Culture medium

MEDIUM 40- for Lactobacillus and Leuconostoc

[Ref.: #33686]

Culture medium growth

[Ref.: #33686]

Culture medium composition

expand / minimize

[Ref.: #121566]

Culture medium

CIP Medium 40

[Ref.: #121566]

Culture medium growth

[Ref.: #121566]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #8248]		growth	28 °C
[Ref.: #32562]		growth	15-45 °C
[Ref.: #32562]		optimum	15-45 °C
[Ref.: #33686]		growth	37 °C
[Ref.: #67770]		growth	28 °C
[Ref.: #121566]		growth	25-37 °C
[Ref.: #121566]	no	growth	15 °C
[Ref.: #121566]	no	growth	45 °C

[Ref.: #8248]	Temperature range	mesophilic
[Ref.: #33686]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #121566]	Temperature range	mesophilic
[Ref.: #121566]	Temperature range	psychrophilic
[Ref.: #121566]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #32562]			optimum	3.3-8
[Ref.: #32562]			growth	3.3-8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #8248]	Oxygen tolerance	microaerophile
[Ref.: #32562]	Oxygen tolerance	anaerobe
[Ref.: #121566]	Oxygen tolerance	facultative anaerobe

[Ref.: #32562]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.995

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #32562]		NaCl		growth	5	%
[Ref.: #32562]		NaCl		optimum	5	%

[Ref.: #8248]	Murein short key	A11.31
[Ref.: #8248]	Murein types	A4alpha L-Lys-D-Asp

[Ref.: #32562]

Observation

aggregates in chains

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #32562]	citrate ChEBI	+	carbon source
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #32562]	esculin ChEBI	+	hydrolysis
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #32562]	malate ChEBI	+	carbon source
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #121566]	nitrate ChEBI	-	reduction
[Ref.: #121566]	nitrate ChEBI	+	respiration
[Ref.: #121566]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #121566]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #121566]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #121566]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #121566]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #121566]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #121566]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #121566]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #121566]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #8248]	14400	-	-	-	-	-	+	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	+	+	+	+	+	+	+	-	-	+	+	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	+	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121566]	12224	-	+	-	-	-	-	-	-	-	-	+	+	+	-	-	+	+	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8248]	Sample type/isolated from	Spanish Bobal grape must
[Ref.: #8248]	Geographic location (country and/or sea, region)	Cuevas de Utiel
[Ref.: #8248]	Country	Spain
[Ref.: #8248]	Country ISO 3 Code	ESP
[Ref.: #8248]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Spanish Bobal grape must

[Ref.: #121566]	Sample type/isolated from	Bobal grape must
[Ref.: #121566]	Country	Spain
[Ref.: #121566]	Country ISO 3 Code	ESP
[Ref.: #121566]	Continent	Europe
[Ref.: #121566]	Isolation date	1997
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Beverage
#Host	#Plants	#Shrub (Scrub)
#Host Body-Site	#Plant	#Fruit (Seed)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY681134 (>99% sequence identity) for Companilactobacillus paralimentarius from Microbeatlas

Aquatic Samples	52
Soil Samples	72
Animal Samples	569
Plant Samples	45
Total Samples	738

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8248]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #121566]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #8248]	Lactobacillus paralimentarius strain 203 16S ribosomal RNA gene, complete sequence	AY681134	1527	ENA	83526 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Companilactobacillus bobalius KACC 16343	GCA_002179915	contig	GenBank	2801451 tax ID
[Ref.: #67770]	Companilactobacillus bobalius DSM 19674	GCA_001434855	scaffold	GenBank	1423788 tax ID
[Ref.: #67770]	Companilactobacillus paralimentarius NBRC 107149	GCA_007991895	contig	GenBank	83526 tax ID
[Ref.: #66792]	Lactobacillus paralimentarius DSM 19674	1423788.3	wgs	PATRIC	1423788 tax ID	*
[Ref.: #66792]	Lactobacillus paralimentarius strain KACC 16343	83526.4	wgs	PATRIC	83526 tax ID	*
[Ref.: #66792]	Lactobacillus paralimentarius strain NBRC 107149	83526.11	wgs	PATRIC	83526 tax ID	*

[Ref.: #8248]	GC-content	36.8 mol%	high performance liquid chromatography (HPLC)
[Ref.: #8248]	GC-content	34 mol%
[Ref.: #67770]	GC-content	35.3 mol%	genome sequence analysis
[Ref.: #67770]	GC-content	34.03 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Lactobacillus paralimentarius strain NBRC 107149 PATRIC: 83526.11
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Lactobacillus paralimentarius DSM 19674 PATRIC: 1423788.3
[Ref.: #69480]	motile	no	95.57	yes
[Ref.: #69480]	gram-positive	yes	94.581	no
[Ref.: #69480]	anaerobic	no	85.427	no
[Ref.: #69480]	halophile	yes	90.815	no
[Ref.: #69480]	spore-forming	no	94.01	yes
[Ref.: #69480]	glucose-util	yes	91.161	no
[Ref.: #69480]	aerobic	no	96.367	yes
[Ref.: #69480]	flagellated	no	98.209	yes
[Ref.: #69480]	glucose-ferment	yes	89.886	no
[Ref.: #69480]	thermophile	no	99.699	yes

Availability in culture collections External links

[Ref.: #8248]

Culture collection no.

DSM 19674, CECT 7310, JCM 16180, CIP 109926, KACC 16343, LMG 25239, NBRC 107149

[Ref.: #76053]

SI-ID 360273

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Reclassification of Lactobacillus kimchii and Lactobacillus bobalius as later subjective synonyms of Lactobacillus paralimentarius.	Pang H, Kitahara M, Tan Z, Wang Y, Qin G, Ohkuma M, Cai Y	Int J Syst Evol Microbiol	10.1099/ijs.0.035329-0	2011	*
Phylogeny	Lactobacillus bobalius sp. nov., a lactic acid bacterium isolated from Spanish Bobal grape must.	Manes-Lazaro R, Ferrer S, Rodas AM, Urdiain M, Pardo I	Int J Syst Evol Microbiol	10.1099/ijs.0.65695-0	2008	*

References

#8248

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19674

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#32562

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28778 (see below)

#33686 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#76053

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID360273.1 )

#121566 Collection of Institut Pasteur ; Curators of the CIP; CIP 109926

#28778

IJSEM 2699 2008 ( DOI 10.1099/ijs.0.65695-0 , PubMed 19060043 )

* These data were automatically processed and therefore are not curated

Change proposal

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Lactobacillus paralimentarius strain NBRC 107149

Lactobacillus paralimentarius DSM 19674

Change proposal

Change proposal