Name: Lactobacillus kefiranofaciens subsp. kefirgranum 1701
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 6566

Type strain:

Species: Lactobacillus kefiranofaciens subsp. kefirgranum

Strain Designation: 1701

Culture col. no.: DSM 10550, JCM 8572, CCUG 49353, ATCC 51647, LMG 19819, CIP 104241, LMG 15132, GCL 1701, CCUG 39467, CECT 5919, CGMCC 1.3398, CGMCC 1.3689, KCTC 5086, LMG 19150, NCIMB 13720

Strain history: CIP <- 1994, JCM <- GCL: strain 1701

NCBI tax ID(s): 1423828 (strain), 190906 (subspecies)

SI-ID 7932

LMG 15132, CIP 104241, JCM 8572, LMG 19150, CCUG 39467, DSM 10550, LMG 19819, ATCC 51647, NCIMB 13720, CECT 5919, CCUG 49353, KCTC 5086

Special Collections

Other strains from Lactobacillus kefiranofaciens subsp. kefirgranum

Section

Lactobacillus kefiranofaciens subsp. kefirgranum 1701 is an anaerobe, Gram-positive bacterium that was isolated from kefir grain.

Gram-positive
anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Lactobacillus*
	Species	**Lactobacillus kefiranofaciens subsp. kefirgranum**
	Full Scientific Name (LPSN)	Lactobacillus kefiranofaciens subsp. kefirgranum (Takizawa et al. 1994) Vancanneyt et al. 2004

Synonym

Lactobacillus kefirgranum

Information on morphological and physiological properties Morphology

[Ref.: #120397]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %94.38

[Ref.: #120397]	Cell shape	rod-shaped

[Ref.: #120397]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %93.5

Information on culture and growth conditions Culture and growth conditions

[Ref.: #3923]

Culture medium

R - CW MEDIUM (DSMZ Medium 775)

[Ref.: #3923]

Culture medium growth

[Ref.: #3923]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3923]

Culture medium composition

expand / minimize

[Ref.: #40101]

Culture medium

MEDIUM 40- for Lactobacillus and Leuconostoc

[Ref.: #40101]

Culture medium growth

[Ref.: #40101]

Culture medium composition

expand / minimize

[Ref.: #120397]

Culture medium

CIP Medium 40

[Ref.: #120397]

Culture medium growth

[Ref.: #120397]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #3923]		growth	30 °C
[Ref.: #40101]		growth	30 °C
[Ref.: #58522]		growth	30 °C
[Ref.: #67770]		growth	30 °C
[Ref.: #120397]		growth	15-37 °C
[Ref.: #120397]	no	growth	45 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #3923]	Oxygen tolerance	anaerobe
[Ref.: #58522]	Oxygen tolerance	anaerobe
[Ref.: #120397]	Oxygen tolerance	facultative anaerobe

[Ref.: #69480]	Ability of spore formation	no Confidence in %90.482
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

[Ref.: #3923]	Murein short key	A11.31
[Ref.: #3923]	Murein types	A4alpha L-Lys-D-Asp

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	cellobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #120397]	esculin ChEBI	+	hydrolysis
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #120397]	nitrate ChEBI	-	reduction
[Ref.: #120397]	nitrate ChEBI	+	respiration
[Ref.: #120397]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #120397]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #120397]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #120397]	catalase	-	1.11.1.6
[Ref.: #120397]	lysine decarboxylase	-	4.1.1.18
[Ref.: #120397]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #120397]	oxidase	-

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #3923]	14283	-	-	-	-	-	-	-	-	-	-	+	+	+	+	-	-	-	-	-	-	-	-	+	+/-	+/-	+	+	+	+	-	-	+/-	+	-	-	+/-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-
[Ref.: #120397]	9546	not determinedn.d.	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	+	-	-	+	-	+	+/-	+/-	-	-	+	-	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	+/-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #3923]	Sample type/isolated from	kefir grain
[Ref.: #3923]	Country	Japan
[Ref.: #3923]	Country ISO 3 Code	JPN
[Ref.: #3923]	Continent	Asia

[Ref.: #58522]	Sample type/isolated from	Kefir grains from E.C.Hansen's lab.,Copenhagen,DK

[Ref.: #67770]	Sample type/isolated from	Kefir grains

[Ref.: #120397]	Sample type/isolated from	Plant, Kefir grains
[Ref.: #120397]	Geographic location (country and/or sea, region)	Copenhagen
[Ref.: #120397]	Country	Denmark
[Ref.: #120397]	Country ISO 3 Code	DNK
[Ref.: #120397]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered

#Food production

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC096210 (>99% sequence identity) for Lactobacillus kefiranofaciens subclade from Microbeatlas

Aquatic Samples	146
Soil Samples	80
Animal Samples	4348
Plant Samples	59
Total Samples	4633

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #3923]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120397]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Lactobacillus kefiranofaciens subsp. kefirgranum gene for 16S ribosomal RNA, partial sequence, strain: JCM 8572	LC096210	1503	GenBank ENA	190906 tax ID
[Ref.: #20218]	Lactobacillus kefirgranum ATCC 51647 16S ribosomal RNA gene, partial sequence	AF429516	505	GenBank ENA	190906 tax ID	*
[Ref.: #20218]	Lactobacillus kefirgranum strain ATCC 51647 16S ribosomal RNA gene, partial sequence; 16S-23S intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	AF429603	513	GenBank ENA	190906 tax ID	*
[Ref.: #20218]	Lactobacillus kefirgranum 16S rRNA gene, strain CECT 5919T	AJ575742	1541	GenBank ENA	190906 tax ID	*
[Ref.: #20218]	Lactobacillus kefiranofaciens subsp. kefirgranum partial 16S rRNA gene, type strain JCM 8572T	FR683093	1448	GenBank ENA	190906 tax ID	*
[Ref.: #20218]	Lactobacillus kefiranofaciens subsp. kefirgranum partial 16S rRNA gene, strain type strain:DSM 10550	AM113782	1551	GenBank ENA	190906 tax ID	*
[Ref.: #20218]	Lactobacillus kefiranofaciens subsp. kefirgranum gene for 16S rRNA, partial sequence, strain: JCM 8572	AB289186	652	GenBank ENA	190906 tax ID	*
[Ref.: #20218]	Lactobacillus kefiranofaciens subsp. kefirgranum 16S rRNA gene, strain LMG 15132	AJ575261	1516	GenBank ENA	190905 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Lactobacillus kefiranofaciens subsp. kefirgranum DSM 10550 = JCM 8572	GCA_001434195	scaffold	GenBank ENA	1423828 tax ID
[Ref.: #66792]	Lactobacillus kefiranofaciens subsp. kefirgranum DSM 10550 = JCM 8572	GCA_001311335	contig	GenBank ENA	1423828 tax ID	*
[Ref.: #66792]	Lactobacillus kefiranofaciens subsp. kefirgranum DSM 10550 = JCM 8572	1423828.4	wgs	PATRIC	1423828 tax ID	*
[Ref.: #66792]	Lactobacillus kefiranofaciens subsp. kefirgranum DSM 10550 = JCM 8572	1423828.3	wgs	PATRIC	1423828 tax ID	*
[Ref.: #66792]	Lactobacillus kefiranofaciens kefirgranum DSM 10550	2675903544	draft	JGI IMG/M	1423828 tax ID	*
[Ref.: #66792]	Lactobacillus kefiranofaciens kefirgranum JCM 8572	2728369681	draft	JGI IMG/M	1423828 tax ID	*

[Ref.: #3923]	GC-content	38.1 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67770]	GC-content	37.5 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Lactobacillus kefiranofaciens subsp. kefirgranum DSM 10550 = JCM 8572 PATRIC: 1423828.3
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Lactobacillus kefiranofaciens subsp. kefirgranum DSM 10550 = JCM 8572 PATRIC: 1423828.4
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	94.38	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	69.212	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	90.482	no
[Ref.: #69480]	aerobic	aerobicⓘ	no	97.591	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	97	no
[Ref.: #69480]	flagellated	motile2+ⓘ	no	93.5	no

Availability in culture collections External links

[Ref.: #3923]

Culture collection no.

DSM 10550, JCM 8572, CCUG 49353, ATCC 51647, LMG 19819, CIP 104241, LMG 15132, GCL 1701, CCUG 39467, CECT 5919, CGMCC 1.3398, CGMCC 1.3689, KCTC 5086, LMG 19150, NCIMB 13720

[Ref.: #76038]	SI-ID 7932	*
[Ref.: #76039]	SI-ID 312267	*
[Ref.: #76040]	SI-ID 312358	*

References

#3923

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 10550

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#40101 ; Curators of the CIP;
#58522 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 49353

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#76038

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID7932.1 )

#76039

#76040

#120397 Collection of Institut Pasteur ; Curators of the CIP; CIP 104241
* These data were automatically processed and therefore are not curated

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Lactobacillus kefiranofaciens subsp. kefirgranum DSM 10550 = JCM 8572

Lactobacillus kefiranofaciens subsp. kefirgranum DSM 10550 = JCM 8572

Change proposal

Change proposal