Lactobacillus kefiranofaciens subsp. kefirgranum (DSM 10550, JCM 8572, CCUG 49353)

Name: Lactobacillus kefiranofaciens subsp. kefirgranum (DSM 10550, JCM 8572, CCUG 49353)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6566

Type strain

Strain Designation 1701

Culture col. no. DSM 10550 JCM 8572 CCUG 49353 ATCC 51647 LMG 19819 11 more

NCBI tax ID(s) 1423828 190906

Special Collections DSMZ CCUG JCM KCTC CIP

History <- JCM <- S. Takizawa, GCL 1701 S. Takizawa GCL 1701. CIP <- 1994, JCM <- GCL: strain 1701

Links

Lactobacillus kefiranofaciens subsp. kefirgranum 1701 is an anaerobe, Gram-positive, rod-shaped bacterium that was isolated from kefir grain.

Gram-positive rod-shaped anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 7932

LMG 15132,CIP 104241,JCM 8572,LMG 19150,CCUG 39467,DSM 10550,LMG 19819,ATCC 51647,NCIMB 13720,CECT 5919,CCUG 49353,KCTC 5086

@ref 20215

Last LPSN update 2026-02-09 11:19:15

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Lactobacillaceae

Genus Lactobacillus

Species Lactobacillus kefiranofaciens subsp. kefirgranum

Full scientific name Lactobacillus kefiranofaciens subsp. kefirgranum (Takizawa et al. 1994) Vancanneyt et al. 2004

Synonyms (1)

Lactobacillus kefirgranum

BacDive ID	Other strains from **Lactobacillus kefiranofaciens subsp. kefirgranum** (11)	Type strain
165821	L. kefiranofaciens subsp. kefirgranum JCM 8570
165822	L. kefiranofaciens subsp. kefirgranum JCM 8571
165970	L. kefiranofaciens subsp. kefirgranum JCM 8940
165971	L. kefiranofaciens subsp. kefirgranum JCM 8941
165972	L. kefiranofaciens subsp. kefirgranum JCM 8942
165973	L. kefiranofaciens subsp. kefirgranum JCM 8943
165974	L. kefiranofaciens subsp. kefirgranum JCM 8944
165975	L. kefiranofaciens subsp. kefirgranum JCM 8945
165976	L. kefiranofaciens subsp. kefirgranum JCM 8946
165977	L. kefiranofaciens subsp. kefirgranum JCM 8947
165978	L. kefiranofaciens subsp. kefirgranum JCM 8948

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
120397	positive	rod-shaped
125438			93.5
125438	positive		94.38

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
3923	R - CW MEDIUM (DSMZ Medium 775)	Medium recipe at MediaDive	Name: R - CW MEDIUM (DSMZ Medium 775) Composition: Yeast extract 5.0 g/l Na-acetate 5.0 g/l KH2PO4 5.0 g/l Trypticase 5.0 g/l Tryptone 5.0 g/l (NH4)2 citrate 2.0 g/l MgSO4 x 7 H2O 0.5 g/l MnSO4 x H2O 0.2 g/l Tween 80 Cheese whey
40101	MEDIUM 40- for Lactobacillus and Leuconostoc		Distilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
120397	CIP Medium 40	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
3923	positive	growth	30
40101	positive	growth	30
58522	positive	growth	30
67770	positive	growth	30
120397	positive	growth	15-37
120397	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
3923	anaerobe
58522	anaerobe
120397	facultative anaerobe
125439	obligate aerobe	98

Spore formation

@ref	Spore formation	Confidence
125438		90.482

Murein

@ref	Murein short key	Type
3923	A11.31	A4alpha L-Lys-D-Asp

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	17057 ChEBI	cellobiose	+	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	+	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
120397	4853 ChEBI	esculin	+	hydrolysis
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
120397	17632 ChEBI	nitrate	-	reduction
120397	17632 ChEBI	nitrate	+	respiration
120397	16301 ChEBI	nitrite	-	reduction
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	+	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
120397	15688 ChEBI	acetoin	-

Enzymes

@ref	Value	Activity	Ec
120397	alcohol dehydrogenase	-	1.1.1.1
120397	catalase	-	1.11.1.6
120397	lysine decarboxylase	-	4.1.1.18
120397	ornithine decarboxylase	-	4.1.1.17
120397	oxidase	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
3923	-	-	-	-	-	-	-	-	-	-	+	+	+	+	-	-	-	-	-	-	-	-	+	+/-	+/-	+	+	+	+	-	-	+/-	+	-	-	+/-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-
120397	not determinedn.d.	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	+	-	-	+	-	+	+/-	+/-	-	-	+	-	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	+/-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Engineered	#Food production

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
3923	kefir grain		Japan	JPN	Asia
58522	Kefir grains from E.C.Hansen's lab.,Copenhagen,DK
67770	Kefir grains
120397	Plant, Kefir grains	Copenhagen	Denmark	DNK	Europe

Taxonmaps

69479

Global distribution of 16S sequence LC096210 (>99% sequence identity) for Lactobacillus kefiranofaciens subclade from Microbeatlas

Aquatic Samples	146
Soil Samples	80
Animal Samples	4348
Plant Samples	59
Total Samples	4633

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
3923	1	Risk group (German classification) According to TRBA 466
120397	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
124043	ASM3953562v1 assembly for Lactobacillus kefiranofaciens subsp. kefirgranum JCM 8572	scaffold	GCA_039535625			190906	62.09
67770	ASM143419v1 assembly for Lactobacillus kefiranofaciens subsp. kefirgranum DSM 10550 = JCM 8572	scaffold	GCA_001434195	1423828.4	2675903544	1423828	50.58
66792	ASM131133v1 assembly for Lactobacillus kefiranofaciens subsp. kefirgranum DSM 10550 = JCM 8572	contig	GCA_001311335	1423828.3	2728369681	1423828

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Lactobacillus kefirgranum ATCC 51647 16S ribosomal RNA gene, partial sequence	AF429516	505	190906
20218	Lactobacillus kefirgranum strain ATCC 51647 16S ribosomal RNA gene, partial sequence; 16S-23S intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	AF429603	513	190906
20218	Lactobacillus kefirgranum 16S rRNA gene, strain CECT 5919T	AJ575742	1541	190906
20218	Lactobacillus kefiranofaciens subsp. kefirgranum partial 16S rRNA gene, type strain JCM 8572T	FR683093	1448	190906
20218	Lactobacillus kefiranofaciens subsp. kefirgranum partial 16S rRNA gene, strain type strain:DSM 10550	AM113782	1551	190906
20218	Lactobacillus kefiranofaciens subsp. kefirgranum gene for 16S rRNA, partial sequence, strain: JCM 8572	AB289186	652	190906
20218	Lactobacillus kefiranofaciens subsp. kefirgranum 16S rRNA gene, strain LMG 15132	AJ575261	1516	190905
67770	Lactobacillus kefiranofaciens subsp. kefirgranum gene for 16S ribosomal RNA, partial sequence, strain: JCM 8572	LC096210	1503	190906

GC content

@ref	GC-content (mol%)	Method
3923	38.1	thermal denaturation, midpoint method (Tm)
67770	37.5

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Lactobacillus kefiranofaciens subsp. kefirgranum DSM 10550 = JCM 8572
NCBI: GCA_001434195

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	63.40	no
125439	motility	BacteriaNetⓘ	no	53.40	no
125439	gram_stain	BacteriaNetⓘ	positive	82.70	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.00	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Lactobacillus kefiranofaciens subsp. kefirgranum DSM 10550 = JCM 8572
PATRIC: 1423828.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	94.38	no
125438	anaerobic	anaerobicⓘ	no	69.21	no
125438	spore-forming	spore-formingⓘ	no	90.48	no
125438	aerobic	aerobicⓘ	no	97.59	no
125438	thermophilic	thermophileⓘ	no	97.00	no
125438	flagellated	motile2+ⓘ	no	93.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Comparative Genomics Analysis of Habitat Adaptation by Lactobacillus kefiranofaciens.	Luo R, Liu C, Li Y, Liu Q, Su X, Peng Q, Lei X, Li W, Menghe B, Bao Q, Liu W.	Foods	10.3390/foods12081606	2023
Biotechnology	A glimpse of antimicrobial resistance gene diversity in kefir and yoghurt.	Toth AG, Csabai I, Maroti G, Jerzsele A, Dubecz A, Patai AV, Judge MF, Nagy SA, Makrai L, Banyai K, Szita G, Solymosi N.	Sci Rep	10.1038/s41598-020-80444-5	2020
Genetics	Microbial Succession and Flavor Production in the Fermented Dairy Beverage Kefir.	Walsh AM, Crispie F, Kilcawley K, O'Sullivan O, O'Sullivan MG, Claesson MJ, Cotter PD.	mSystems	10.1128/msystems.00052-16	2016
	Analysis of Health Benefits Conferred by Lactobacillus Species from Kefir.	Slattery C, Cotter PD, O'Toole PW.	Nutrients	10.3390/nu11061252	2019
	Amplicon Sequencing of the slpH Locus Permits Culture-Independent Strain Typing of Lactobacillus helveticus in Dairy Products.	Moser A, Wuthrich D, Bruggmann R, Eugster-Meier E, Meile L, Irmler S.	Front Microbiol	10.3389/fmicb.2017.01380	2017
Genetics	Unveiling the Brazilian kefir microbiome: discovery of a novel Lactobacillus kefiranofaciens (LkefirU) genome and in silico prospection of bioactive peptides with potential anti-Alzheimer properties.	Silva MH, Batista LL, Malta SM, Santos ACC, Mendes-Silva AP, Bonetti AM, Ueira-Vieira C, Dos Santos AR.	BMC Genomics	10.1186/s12864-024-10695-3	2024
	Verticle transmission of unique bacterial strains from mother to infant via consuming natural kefir	Tunay RT, Kok Tas T.	Int Dairy J		2022
	Effect of Milk and Water Kefir Grains on the Nutritional Profile and Antioxidant Capacity of Fermented Almond Milk.	La Torre C, Caputo P, Fazio A.	Molecules	10.3390/molecules30030698	2025
Pathogenicity	Modulation of the Gut-Lung Axis by Water Kefir and Kefiran and Their Impact on Toll-like Receptor 3-Mediated Respiratory Immunity.	Dentice Maidana S, Arganaraz Aybar JN, Albarracin L, Imamura Y, Arellano-Arriagada L, Namai F, Suda Y, Nishiyama K, Villena J, Kitazawa H.	Biomolecules	10.3390/biom14111457	2024
Phylogeny	Subspecies Classification and Comparative Genomic Analysis of Lactobacillus kefiranofaciens HL1 and M1 for Potential Niche-Specific Genes and Pathways.	Wang SY, Chen YP, Huang RF, Wu YL, Ho ST, Li KY, Watanabe K, Chen MJ.	Microorganisms	10.3390/microorganisms10081637	2022
	Therapeutic effects of kefir grain Lactobacillus-derived extracellular vesicles in mice with 2,4,6-trinitrobenzene sulfonic acid-induced inflammatory bowel disease.	Seo MK, Park EJ, Ko SY, Choi EW, Kim S.	J Dairy Sci	10.3168/jds.2018-15014	2018
	Bioactive Compounds from Kefir and Their Potential Benefits on Health: A Systematic Review and Meta-Analysis.	Vieira CP, Rosario AILS, Lelis CA, Rekowsky BSS, Carvalho APA, Rosario DKA, Elias TA, Costa MP, Foguel D, Conte-Junior CA.	Oxid Med Cell Longev	10.1155/2021/9081738	2021
	Lactobacillus kefiranofaciens: From Isolation and Taxonomy to Probiotic Properties and Applications.	Georgalaki M, Zoumpopoulou G, Anastasiou R, Kazou M, Tsakalidou E.	Microorganisms	10.3390/microorganisms9102158	2021
	The Many Faces of Kefir Fermented Dairy Products: Quality Characteristics, Flavour Chemistry, Nutritional Value, Health Benefits, and Safety.	Farag MA, Jomaa SA, El-Wahed AA, El-Seedi AHR.	Nutrients	10.3390/nu12020346	2020
	New cocoa pulp-based kefir beverages: Microbiological, chemical composition and sensory analysis	Puerari C, MagalhAPSes KT, Schwan RF.	Food Res Int	10.1016/j.foodres.2012.06.005	2012
	Identification of Potential Bioactive Peptides in Sheep Milk Kefir through Peptidomic Analysis at Different Fermentation Times.	Dalabasmaz S, de la Torre EP, Gensberger-Reigl S, Pischetsrieder M, Rodriguez-Ortega MJ.	Foods	10.3390/foods12152974	2023
	Effect of administering kefir on the changes in fecal microbiota and symptoms of inflammatory bowel disease: A randomized controlled trial.	Yilmaz I, Dolar ME, Ozpinar H.	Turk J Gastroenterol	10.5152/tjg.2018.18227	2019
Biotechnology	Immunofluorescence microscopic studies on distribution of Lactobacillus kefiranofaciens and Lactobacillus kefir in kefir grains.	Arihara K, Toba T, Adachi S.	Int J Food Microbiol	10.1016/0168-1605(90)90047-9	1990
	Effects of Kefir on the Cardiac Autonomic Tones and Baroreflex Sensitivity in Spontaneously Hypertensive Rats.	Klippel BF, Duemke LB, Leal MA, Friques AG, Dantas EM, Dalvi RF, Gava AL, Pereira TM, Andrade TU, Meyrelles SS, Campagnaro BP, Vasquez EC.	Front Physiol	10.3389/fphys.2016.00211	2016
	Mechanistic Insights Into Probiotic Properties of Lactic Acid Bacteria Associated With Ethnic Fermented Dairy Products.	Ghosh T, Beniwal A, Semwal A, Navani NK.	Front Microbiol	10.3389/fmicb.2019.00502	2019
	Precision Postbiotics and Mental Health: the Management of Post-COVID-19 Complications.	Pandey M, Bhati A, Priya K, Sharma KK, Singhal B.	Probiotics Antimicrob Proteins	10.1007/s12602-021-09875-4	2022
Phylogeny	Reclassification of Lactobacillus kefirgranum Takizawa et al. 1994 as Lactobacillus kefiranofaciens subsp. kefirgranum subsp. nov. and emended description of L. kefiranofaciens Fujisawa et al. 1988.	Vancanneyt M, Mengaud J, Cleenwerck I, Vanhonacker K, Hoste B, Dawyndt P, Degivry MC, Ringuet D, Janssens D, Swings J.	Int J Syst Evol Microbiol	10.1099/ijs.0.02912-0	2004

References

#3923	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 10550
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#40101	; Curators of the CIP;
#58522	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 49353
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#120397	Collection of Institut Pasteur ; Curators of the CIP; CIP 104241
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Lactobacillus kefiranofaciens subsp. kefirgranum (DSM 10550, JCM 8572, CCUG 49353)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Murein

Metabolite utilization

Metabolite tests

Enzymes

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_039535625

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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