Name: Limosilactobacillus vaginalis Lac 19
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6558

Type strain:

Species: Limosilactobacillus vaginalis

Strain Designation: Lac 19

Culture col. no.: DSM 5837, ATCC 49540, NCTC 12197, CCUG 31452, JCM 9505, BCRC 14735, CECT 4089, CIP 105932, KCTC 3515, LMG 12891, NCFB 2810, NCIMB 702810

Strain history: NCTC 12197 <-- T. M. Embley Lac 19 <-- W. Bossart.

NCBI tax ID(s): 1633 (species)

Other strains from Limosilactobacillus vaginalis

L. vaginalis Cla-CZ-13, DSM 108982
L. vaginalis JCM 7771

Section

Limosilactobacillus vaginalis Lac 19 is an anaerobe, mesophilic bacterium that was isolated from vaginal swab from patient with trichomoniasis.

anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Limosilactobacillus*
	Species	**Limosilactobacillus vaginalis**
	Full Scientific Name (LPSN)	Limosilactobacillus vaginalis (Embley et al. 1989) Zheng et al. 2020

Synonym

Lactobacillus vaginalis

Information on culture and growth conditions Culture and growth conditions

[Ref.: #2334]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #2334]

Culture medium growth

[Ref.: #2334]

Culture medium link

Medium recipe at MediaDive

[Ref.: #2334]

Culture medium composition

expand / minimize

[Ref.: #38591]

Culture medium

MEDIUM 40- for Lactobacillus and Leuconostoc

[Ref.: #38591]

Culture medium growth

[Ref.: #38591]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #2334]	growth	37 °C
[Ref.: #38591]	growth	37 °C
[Ref.: #50607]	growth	37 °C
[Ref.: #50607]	growth	37-42 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #2334]	Temperature range	mesophilic
[Ref.: #38591]	Temperature range	mesophilic
[Ref.: #50607]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #50607]	Oxygen tolerance	anaerobe
[Ref.: #50607]	Oxygen tolerance	microaerophile

[Ref.: #2334]	Murein short key	A21.04
[Ref.: #2334]	Murein types	A4ß L-Orn-D-Asp

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68377]	D-fructose ChEBI	+	builds acid from	* from API NH, API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68377]	D-glucose ChEBI	+	builds acid from	* from API NH, API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68377]	maltose ChEBI	+	builds acid from	* from API NH, API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68377]	ornithine ChEBI	-	degradation	* from API NH
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68377]	sucrose ChEBI	-	builds acid from	* from API NH
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68377]	tryptophan ChEBI	-	energy source	* from API NH
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68377]	urea ChEBI	-	hydrolysis	* from API NH
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite
[Ref.: #68377]		indole ChEBI	* from API NH

	Physiological tests	Metabolite	Indole test
[Ref.: #68377]		indole ChEBI	-	* from API NH

	Enzyme	Enzyme activity	EC number
[Ref.: #68377]	alkaline phosphatase	-	3.1.3.1	* from API NH
[Ref.: #68377]	beta-galactosidase	-	3.2.1.23	* from API NH
[Ref.: #68377]	beta-lactamase	-	3.5.2.6	* from API NH
[Ref.: #68377]	gamma-glutamyltransferase	-	2.3.2.2	* from API NH
[Ref.: #68377]	lipase	+		* from API NH
[Ref.: #68377]	ornithine decarboxylase	-	4.1.1.17	* from API NH
[Ref.: #68377]	proline-arylamidase	-	3.4.11.5	* from API NH
[Ref.: #68377]	tryptophan deaminase	-	4.1.99.1	* from API NH
[Ref.: #68377]	urease	-	3.5.1.5	* from API NH

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #50607]

Fatty acid	Percentage	ECL
C18:1 ω9c	33.3	17.769
C16:0	23.7	16
C19:0 CYCLO ω9c	15.7	18.87
C18:1 ω7c /12t/9t	15.5	17.824
C14:0	7.4	14
C16:1 ω7c	4.4	15.819

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #2334]	7896	-	-	-	-	-	-	-	-	-	-	+	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #2334]	7895	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	+	+	+	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #2334]	7894	-	-	-	-	-	-	-	-	-	-	+	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API® NH:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Penicillinase productionPEN	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from maltoseMAL	Acid from sucroseSAC	Ornithine decarboxylaseODC	Urease/urea hydrolysisURE	LipaseLIP	Alkaline phosphatasePAL	beta-Galactosidasebeta GAL	Proline arylamidaseProA	gamma-Glutamyl transferaseGGT	Indole productionIND
[Ref.: #50607]	1431	-	+	+	+	-	-	-	+	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #2334]	Sample type/isolated from	vaginal swab from patient with trichomoniasis

[Ref.: #50607]	Sample type/isolated from	Human vagina,patient with trichomoniasis

[Ref.: #67770]	Sample type/isolated from	Vagina of patients suffering from trichomonaiasis
* marker position based on {}

Isolation sources categories

#Infection	#Disease	-
#Infection	#Patient	-
#Infection	#Patient	#Swab
#Host Body-Site	#Urogenital tract	#Vagina

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC096217 (>99% sequence identity) for Limosilactobacillus from Microbeatlas

Aquatic Samples	719
Soil Samples	1438
Animal Samples	55246
Plant Samples	398
Total Samples	57801

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #2334]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Lactobacillus vaginalis gene for 16S ribosomal RNA, partial sequence, strain: JCM 9505	LC096217	1513	ENA	1633 tax ID
[Ref.: #20218]	Lactobacillus vaginalis strain ATCC49540 16S ribosomal RNA gene, partial sequence	AF243177	1541	ENA	1633 tax ID	*
[Ref.: #20218]	Lactobacillus vaginalis strain DSM 5837 16S-23S ribosomal RNA intergenic spacer and 23S ribosomal RNA gene, partial sequence	EU161598	796	ENA	1633 tax ID	*
[Ref.: #20218]	Lactobacillus vaginalis gene for 16S rRNA, partial sequence, strain: JCM 9505	AB289311	648	ENA	1633 tax ID	*
[Ref.: #20218]	Lactobacillus vaginalis 16S ribosomal RNA, partial sequence; 16S/23S intergenic spacer region, complete sequence; and 23S ribosomal RNA partial sequence	AF182731	672	ENA	1633 tax ID	*
[Ref.: #20218]	L.vaginalis 16S rRNA gene	X61136	1488	ENA	1633 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Limosilactobacillus vaginalis DSM 5837 = ATCC 49540 DSM 5837	GCA_001435915	scaffold	GenBank	1423814 tax ID
[Ref.: #67770]	Limosilactobacillus vaginalis DSM 5837 = ATCC 49540 ATCC 49540	GCA_000159435	scaffold	GenBank	1423814 tax ID
[Ref.: #66792]	Lactobacillus vaginalis ATCC 49540	525366.3	wgs	PATRIC	1423814 tax ID	*
[Ref.: #66792]	Lactobacillus vaginalis DSM 5837 = ATCC 49540	1423814.6	wgs	PATRIC	1423814 tax ID	*
[Ref.: #66792]	Lactobacillus vaginalis DSM 5837 = ATCC 49540 strain SRR9217392-mag-bin.4	1423814.24	wgs	PATRIC	1423814 tax ID	*
[Ref.: #66792]	Limosilactobacillus vaginalis ATCC 49540	643886050	draft	JGI IMG/M	1423814 tax ID	*

[Ref.: #2334]	GC-content	39.0 mol%
[Ref.: #67770]	GC-content	40.5 mol%	genome sequence analysis
[Ref.: #67770]	GC-content	39 mol%	thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #2334]

Culture collection no.

DSM 5837, ATCC 49540, NCTC 12197, CCUG 31452, JCM 9505, BCRC 14735, CECT 4089, CIP 105932, KCTC 3515, LMG 12891, NCFB 2810, NCIMB 702810

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Limosilactobacillus urinaemulieris sp. nov. and Limosilactobacillus portuensis sp. nov. isolated from urine of healthy women.	Ksiezarek M, Ribeiro TG, Rocha J, Grosso F, Perovic SU, Peixe L	Int J Syst Evol Microbiol	10.1099/ijsem.0.004726	2021	*
Phylogeny	The genome of the Lactobacillus sanfranciscensis temperate phage EV3.	Ehrmann MA, Angelov A, Picozzi C, Foschino R, Vogel RF	BMC Res Notes	10.1186/1756-0500-6-514	2013	*

References

#2334

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 5837

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#38591 ; Curators of the CIP;
#50607 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 31452

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68377

Automatically annotated from API NH .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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