Cite this strain

Lacticaseibacillus paracasei subsp. paracasei (DSM 20312, ATCC 27092, S)

BacDive ID 6555

Type strain

Strain Designation S

Culture col. no. DSM 20312 ATCC 27092 S

NCBI tax ID(s) 47714

Special Collections DSMZ

History <- K.O. Stetter <- K. Watanabe, strain S (Lactobacillus casei)

Links

Lacticaseibacillus paracasei subsp. paracasei S is a mesophilic prokaryote that was isolated from lactic acid beverage "Yakult".

mesophilic genome sequence

Name and taxonomic classification

SI-ID 38807

ATCC 27092,CECT 4583,CCRC 14023,BCRC 14023,DSM 20312

@ref 20215

Last LPSN update 2026-02-09 11:19:15

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Lactobacillaceae

Genus Lacticaseibacillus

Species Lacticaseibacillus paracasei subsp. paracasei

Full scientific name Lacticaseibacillus paracasei subsp. paracasei (Collins et al. 1989) Zheng et al. 2020

Synonyms (1)

Lactobacillus paracasei subsp. paracasei

BacDive ID	Other strains from **Lacticaseibacillus paracasei subsp. paracasei** (19)	Type strain
6548	L. paracasei subsp. paracasei RO94, DSM 5622, ATCC 25302, NCDO 151, CCUG ... (type strain)
6545	L. paracasei subsp. paracasei No. 59, DSM 2649
6546	L. paracasei subsp. paracasei DSM 4905, ATCC 11578
6549	L. paracasei subsp. paracasei A22/73, DSM 8741, NCFB 2976
6550	L. paracasei subsp. paracasei A112/73, DSM 8742, NCFB 2977
6551	L. paracasei subsp. paracasei 85 E, DSM 20006, JCM 1163, KCTC 3166
6552	L. paracasei subsp. paracasei 40, DSM 20008, ATCC 25598, NCDO 2743, NCIB ...
6553	L. paracasei subsp. paracasei C 17, CCUG 34680, DSM 20020, ATCC 27216, ...
6554	L. paracasei subsp. paracasei 41, DSM 20207, NCDO 1977, JCM 1161
6556	L. paracasei subsp. paracasei 28, DSM 20244
6557	L. paracasei subsp. paracasei 9664, A 13, BU 240, Ep 300, Epsilon, PBR ...
6659	L. paracasei subsp. paracasei B41, DSM 23505
160021	L. paracasei subsp. paracasei GCRL 163, DSM 111790
160981	L. paracasei subsp. paracasei JCM 1053, IAM 1043
161128	L. paracasei subsp. paracasei JCM 1109
161129	L. paracasei subsp. paracasei JCM 1111
163406	L. paracasei subsp. paracasei JCM 2769
165742	L. paracasei subsp. paracasei JCM 8131, NCFB 205, NCIMB 2378, NCTC 2378
165743	L. paracasei subsp. paracasei JCM 8133, NCDO 1202

Morphology

Cell morphology

@ref	Motility	Confidence	Gram stain
125438		91.5
125438		92.404	positive

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
8591	MRS MEDIUM (DSMZ Medium 11)		Medium recipe at MediaDive	Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
8591	positive	growth	30	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	96.1

Spore formation

@ref	Spore formation	Confidence
125439		92.3

Murein

@ref	Murein short key	Type
8591	A11.31	A4alpha L-Lys-D-Asp

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	18305 ChEBI	arbutin	+	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	+	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	+	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	+	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	+	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	+	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	+	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	+	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	+	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	+	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	+	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	+	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	+	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
8591	-	-	-	-	-	+	-	-	+	-	+	+	+	+	+	-	-	-	+	+	-	-	+	+/-	+	+	+	+	+	-	-	-	+	-	-	-	-	-	-	+	+	-	+	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Food production	#Dairy product
#Engineered	#Food production	#Fermented
#Engineered	#Food production	#Beverage

Isolation

8591

Sample typelactic acid beverage "Yakult"

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Biosafety level	Biosafety level comment
8591	yes, in single cases	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	NCBI tax ID	Score
66792	ASM2365696v1 assembly for Lacticaseibacillus paracasei ATCC 27092	contig	GCA_023656965	1597.1018	1597	50.45

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Lacticaseibacillus paracasei ATCC 27092
NCBI: GCA_023656965

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	92.30	no
125439	motility	BacteriaNetⓘ	no	64.30	no
125439	gram_stain	BacteriaNetⓘ	positive	85.10	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	96.10	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Lacticaseibacillus paracasei ATCC 27092
NCBI: GCA_023656965.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	92.40	no
125438	anaerobic	anaerobicⓘ	no	87.82	no
125438	aerobic	aerobicⓘ	no	93.35	no
125438	spore-forming	spore-formingⓘ	no	79.64	no
125438	thermophilic	thermophileⓘ	no	95.50	no
125438	flagellated	motile2+ⓘ	no	91.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Highlighting Lactic Acid Bacteria in Beverages: Diversity, Fermentation, Challenges, and Future Perspectives.	Al-Kharousi ZS.	Foods	10.3390/foods14122043	2025
	What Makes Lupins Less Palatable to Consumers? Can the Sensory Quality of Lupin be Improved and Commercialized?	Wanniarachchi PC, Shea G, Mocerino M, Bennett SJ, Bhattarai RR, Coorey R.	Compr Rev Food Sci Food Saf	10.1111/1541-4337.70265	2025
	Combined acid hydrolysis and fermentation improves bioactivity of citrus flavonoids in vitro and in vivo.	Konig A, Sadova N, Dornmayr M, Schwarzinger B, Neuhauser C, Stadlbauer V, Wallner M, Woischitzschlager J, Muller A, Tona R, Kofel D, Weghuber J.	Commun Biol	10.1038/s42003-023-05424-7	2023
	Legumes and Legume-Based Beverages Fermented with Lactic Acid Bacteria as a Potential Carrier of Probiotics and Prebiotics.	Cichonska P, Ziarno M.	Microorganisms	10.3390/microorganisms10010091	2021
	Phage PL-1 endolysin and osmotic stress as tools to enhance heterologous protein display in lactic acid bacteria platforms	Gordillo TB, Jastrebow IG, De Rossi MC, Da Silva Lima CH, Bockor SS, Allievi MC, Do Porto DF, Palomino MM.	International Journal of Biological Macromolecules.		2025
	Phage PL-1 endolysin and osmotic stress as tools to enhance heterologous protein display in lactic acid bacteria platforms.	Gordillo TB, Jastrebow IG, De Rossi MC, Da Silva Lima CH, Bockor SS, Allievi MC, Do Porto DF, Palomino MM.	Int J Biol Macromol	10.1016/j.ijbiomac.2025.145886	2025
Genetics	Utilization of Native CRISPR-Cas9 System for Expression of Glucagon-like Peptide-1 in Lacticaseibacillus paracasei.	Zheng M, Zhang S, Wang Y, Xie N, Wang X, Lv J, Pang X, Li X.	Foods	10.3390/foods14101785	2025
Genetics	Lacticaseibacillus paracasei AD22 Stress Response in Brined White Cheese Matrix: In Vitro Probiotic Profiles and Molecular Characterization.	Dishan A, Gonulalan Z.	Probiotics Antimicrob Proteins	10.1007/s12602-024-10216-4	2025
	Metabolite profiling and bioactivity guided fractionation of Lactobacillaceae and rice bran postbiotics for antimicrobial-resistant Salmonella Typhimurium growth suppression.	Nealon NJ, Worcester CR, Boyer SM, Haberecht HB, Ryan EP.	Front Microbiol	10.3389/fmicb.2024.1362266	2024
	Metabolomics of Rice Bran Differentially Impacted by Fermentation With Six Probiotics Demonstrates Key Nutrient Changes for Enhancing Gut Health.	Seyoum Y, Humblot C, Baxter BA, Nealon NJ, Weber AM, Ryan EP.	Front Nutr	10.3389/fnut.2021.795334	2021
Pathogenicity	The Complex Role of Lactic Acid Bacteria in Food Detoxification.	Petrova P, Arsov A, Tsvetanova F, Parvanova-Mancheva T, Vasileva E, Tsigoriyna L, Petrov K.	Nutrients	10.3390/nu14102038	2022
	Molecular patterns from a human gut-derived Lactobacillus strain suppress pathogenic infiltration of leukocytes into the central nervous system.	Sanchez JMS, Doty DJ, DePaula-Silva AB, Brown DG, Bell R, Klag KA, Truong A, Libbey JE, Round JL, Fujinami RS.	J Neuroinflammation	10.1186/s12974-020-01959-2	2020
Metabolism	Lactobacillus paracasei metabolism of rice bran reveals metabolome associated with Salmonella Typhimurium growth reduction.	Nealon NJ, Worcester CR, Ryan EP	J Appl Microbiol	10.1111/jam.13459	2017
Phylogeny	Isolation and phenotypic characterization of Lactobacillus casei and Lactobacillus paracasei bacteriophage-resistant mutants.	Capra ML, Mercanti DJ, Rossetti LC, Reinheimer JA, Quiberoni A	J Appl Microbiol	10.1111/j.1365-2672.2011.05056.x	2011
Phylogeny	Widely distributed lysogeny in probiotic lactobacilli represents a potentially high risk for the fermentative dairy industry.	Mercanti DJ, Carminati D, Reinheimer JA, Quiberoni A	Int J Food Microbiol	10.1016/j.ijfoodmicro.2010.11.009	2010
Phylogeny	Comparative analysis of the genes encoding 23S-5S rRNA intergenic spacer regions of Lactobacillus casei-related strains.	Chen H, Lim CK, Lee YK, Chan YN	Int J Syst Evol Microbiol	10.1099/00207713-50-2-471	2000
	Use of Selected Lactic Acid Bacteria for the Fermentation of Legume-Based Water Extracts.	Demarinis C, Verni M, Pinto L, Rizzello CG, Baruzzi F	Foods	10.3390/foods11213346	2022

References

#8591	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20312
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68371	Automatically annotated from API 50CH acid .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Lacticaseibacillus paracasei subsp. paracasei (DSM 20312, ATCC 27092, S)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Murein

Metabolite utilization

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_023656965

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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